Job ID = 6528092 SRX = SRX4510349 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:23:48 prefetch.2.10.7: 1) Downloading 'SRR7647399'... 2020-06-29T14:23:48 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:26:18 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:26:18 prefetch.2.10.7: 1) 'SRR7647399' was downloaded successfully 2020-06-29T14:26:18 prefetch.2.10.7: 'SRR7647399' has 0 unresolved dependencies Read 24091206 spots for SRR7647399/SRR7647399.sra Written 24091206 spots for SRR7647399/SRR7647399.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:07 24091206 reads; of these: 24091206 (100.00%) were unpaired; of these: 2432374 (10.10%) aligned 0 times 18946013 (78.64%) aligned exactly 1 time 2712819 (11.26%) aligned >1 times 89.90% overall alignment rate Time searching: 00:11:07 Overall time: 00:11:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3632026 / 21658832 = 0.1677 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:56:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4510349/SRX4510349.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4510349/SRX4510349.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4510349/SRX4510349.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4510349/SRX4510349.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:56:58: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:56:58: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:57:05: 1000000 INFO @ Mon, 29 Jun 2020 23:57:11: 2000000 INFO @ Mon, 29 Jun 2020 23:57:17: 3000000 INFO @ Mon, 29 Jun 2020 23:57:24: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:57:30: 5000000 INFO @ Mon, 29 Jun 2020 23:57:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4510349/SRX4510349.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4510349/SRX4510349.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4510349/SRX4510349.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4510349/SRX4510349.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:57:30: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:57:30: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:57:37: 6000000 INFO @ Mon, 29 Jun 2020 23:57:37: 1000000 INFO @ Mon, 29 Jun 2020 23:57:44: 7000000 INFO @ Mon, 29 Jun 2020 23:57:44: 2000000 INFO @ Mon, 29 Jun 2020 23:57:51: 8000000 INFO @ Mon, 29 Jun 2020 23:57:51: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:57:57: 9000000 INFO @ Mon, 29 Jun 2020 23:57:58: 4000000 INFO @ Mon, 29 Jun 2020 23:57:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4510349/SRX4510349.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4510349/SRX4510349.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4510349/SRX4510349.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4510349/SRX4510349.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:57:58: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:57:58: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:58:04: 10000000 INFO @ Mon, 29 Jun 2020 23:58:05: 5000000 INFO @ Mon, 29 Jun 2020 23:58:05: 1000000 INFO @ Mon, 29 Jun 2020 23:58:11: 11000000 INFO @ Mon, 29 Jun 2020 23:58:12: 6000000 INFO @ Mon, 29 Jun 2020 23:58:13: 2000000 INFO @ Mon, 29 Jun 2020 23:58:18: 12000000 INFO @ Mon, 29 Jun 2020 23:58:19: 7000000 INFO @ Mon, 29 Jun 2020 23:58:20: 3000000 INFO @ Mon, 29 Jun 2020 23:58:25: 13000000 INFO @ Mon, 29 Jun 2020 23:58:26: 8000000 INFO @ Mon, 29 Jun 2020 23:58:27: 4000000 INFO @ Mon, 29 Jun 2020 23:58:33: 14000000 INFO @ Mon, 29 Jun 2020 23:58:33: 9000000 INFO @ Mon, 29 Jun 2020 23:58:34: 5000000 INFO @ Mon, 29 Jun 2020 23:58:39: 15000000 INFO @ Mon, 29 Jun 2020 23:58:40: 10000000 INFO @ Mon, 29 Jun 2020 23:58:41: 6000000 INFO @ Mon, 29 Jun 2020 23:58:47: 11000000 INFO @ Mon, 29 Jun 2020 23:58:47: 16000000 INFO @ Mon, 29 Jun 2020 23:58:48: 7000000 INFO @ Mon, 29 Jun 2020 23:58:53: 12000000 INFO @ Mon, 29 Jun 2020 23:58:53: 17000000 INFO @ Mon, 29 Jun 2020 23:58:55: 8000000 INFO @ Mon, 29 Jun 2020 23:59:00: 13000000 INFO @ Mon, 29 Jun 2020 23:59:00: 18000000 INFO @ Mon, 29 Jun 2020 23:59:01: #1 tag size is determined as 101 bps INFO @ Mon, 29 Jun 2020 23:59:01: #1 tag size = 101 INFO @ Mon, 29 Jun 2020 23:59:01: #1 total tags in treatment: 18026806 INFO @ Mon, 29 Jun 2020 23:59:01: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:59:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:59:01: #1 tags after filtering in treatment: 18026806 INFO @ Mon, 29 Jun 2020 23:59:01: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:59:01: #1 finished! INFO @ Mon, 29 Jun 2020 23:59:01: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:59:01: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:59:02: 9000000 INFO @ Mon, 29 Jun 2020 23:59:02: #2 number of paired peaks: 57 WARNING @ Mon, 29 Jun 2020 23:59:02: Too few paired peaks (57) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:59:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4510349/SRX4510349.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4510349/SRX4510349.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4510349/SRX4510349.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4510349/SRX4510349.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:59:07: 14000000 INFO @ Mon, 29 Jun 2020 23:59:08: 10000000 INFO @ Mon, 29 Jun 2020 23:59:14: 15000000 INFO @ Mon, 29 Jun 2020 23:59:15: 11000000 INFO @ Mon, 29 Jun 2020 23:59:21: 16000000 INFO @ Mon, 29 Jun 2020 23:59:22: 12000000 INFO @ Mon, 29 Jun 2020 23:59:27: 17000000 INFO @ Mon, 29 Jun 2020 23:59:28: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:59:34: 18000000 INFO @ Mon, 29 Jun 2020 23:59:34: #1 tag size is determined as 101 bps INFO @ Mon, 29 Jun 2020 23:59:34: #1 tag size = 101 INFO @ Mon, 29 Jun 2020 23:59:34: #1 total tags in treatment: 18026806 INFO @ Mon, 29 Jun 2020 23:59:34: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:59:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:59:35: #1 tags after filtering in treatment: 18026806 INFO @ Mon, 29 Jun 2020 23:59:35: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:59:35: #1 finished! INFO @ Mon, 29 Jun 2020 23:59:35: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:59:35: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:59:35: 14000000 INFO @ Mon, 29 Jun 2020 23:59:36: #2 number of paired peaks: 57 WARNING @ Mon, 29 Jun 2020 23:59:36: Too few paired peaks (57) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:59:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4510349/SRX4510349.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4510349/SRX4510349.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4510349/SRX4510349.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4510349/SRX4510349.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:59:42: 15000000 INFO @ Mon, 29 Jun 2020 23:59:48: 16000000 INFO @ Mon, 29 Jun 2020 23:59:54: 17000000 INFO @ Tue, 30 Jun 2020 00:00:01: 18000000 INFO @ Tue, 30 Jun 2020 00:00:01: #1 tag size is determined as 101 bps INFO @ Tue, 30 Jun 2020 00:00:01: #1 tag size = 101 INFO @ Tue, 30 Jun 2020 00:00:01: #1 total tags in treatment: 18026806 INFO @ Tue, 30 Jun 2020 00:00:01: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:00:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:00:01: #1 tags after filtering in treatment: 18026806 INFO @ Tue, 30 Jun 2020 00:00:01: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:00:01: #1 finished! INFO @ Tue, 30 Jun 2020 00:00:01: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:00:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:00:02: #2 number of paired peaks: 57 WARNING @ Tue, 30 Jun 2020 00:00:02: Too few paired peaks (57) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:00:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4510349/SRX4510349.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4510349/SRX4510349.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4510349/SRX4510349.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4510349/SRX4510349.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。