Job ID = 6528088 SRX = SRX450797 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:10:50 prefetch.2.10.7: 1) Downloading 'SRR1145614'... 2020-06-29T14:10:50 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:16:10 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:16:10 prefetch.2.10.7: 1) 'SRR1145614' was downloaded successfully Read 24024711 spots for SRR1145614/SRR1145614.sra Written 24024711 spots for SRR1145614/SRR1145614.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:09 24024711 reads; of these: 24024711 (100.00%) were unpaired; of these: 1180231 (4.91%) aligned 0 times 20324214 (84.60%) aligned exactly 1 time 2520266 (10.49%) aligned >1 times 95.09% overall alignment rate Time searching: 00:11:09 Overall time: 00:11:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5198215 / 22844480 = 0.2275 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:55:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX450797/SRX450797.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX450797/SRX450797.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX450797/SRX450797.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX450797/SRX450797.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:55:07: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:55:07: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:55:14: 1000000 INFO @ Mon, 29 Jun 2020 23:55:22: 2000000 INFO @ Mon, 29 Jun 2020 23:55:29: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:55:36: 4000000 INFO @ Mon, 29 Jun 2020 23:55:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX450797/SRX450797.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX450797/SRX450797.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX450797/SRX450797.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX450797/SRX450797.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:55:37: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:55:37: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:55:44: 5000000 INFO @ Mon, 29 Jun 2020 23:55:44: 1000000 INFO @ Mon, 29 Jun 2020 23:55:51: 2000000 INFO @ Mon, 29 Jun 2020 23:55:52: 6000000 INFO @ Mon, 29 Jun 2020 23:55:59: 3000000 INFO @ Mon, 29 Jun 2020 23:55:59: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:56:06: 4000000 INFO @ Mon, 29 Jun 2020 23:56:07: 8000000 INFO @ Mon, 29 Jun 2020 23:56:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX450797/SRX450797.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX450797/SRX450797.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX450797/SRX450797.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX450797/SRX450797.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:56:07: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:56:07: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:56:13: 5000000 INFO @ Mon, 29 Jun 2020 23:56:15: 9000000 INFO @ Mon, 29 Jun 2020 23:56:15: 1000000 INFO @ Mon, 29 Jun 2020 23:56:21: 6000000 INFO @ Mon, 29 Jun 2020 23:56:23: 10000000 INFO @ Mon, 29 Jun 2020 23:56:24: 2000000 INFO @ Mon, 29 Jun 2020 23:56:28: 7000000 INFO @ Mon, 29 Jun 2020 23:56:31: 11000000 INFO @ Mon, 29 Jun 2020 23:56:32: 3000000 INFO @ Mon, 29 Jun 2020 23:56:35: 8000000 INFO @ Mon, 29 Jun 2020 23:56:40: 12000000 INFO @ Mon, 29 Jun 2020 23:56:40: 4000000 INFO @ Mon, 29 Jun 2020 23:56:42: 9000000 INFO @ Mon, 29 Jun 2020 23:56:48: 13000000 INFO @ Mon, 29 Jun 2020 23:56:48: 5000000 INFO @ Mon, 29 Jun 2020 23:56:50: 10000000 INFO @ Mon, 29 Jun 2020 23:56:56: 14000000 INFO @ Mon, 29 Jun 2020 23:56:57: 6000000 INFO @ Mon, 29 Jun 2020 23:56:57: 11000000 INFO @ Mon, 29 Jun 2020 23:57:04: 12000000 INFO @ Mon, 29 Jun 2020 23:57:05: 15000000 INFO @ Mon, 29 Jun 2020 23:57:05: 7000000 INFO @ Mon, 29 Jun 2020 23:57:11: 13000000 INFO @ Mon, 29 Jun 2020 23:57:13: 16000000 INFO @ Mon, 29 Jun 2020 23:57:13: 8000000 INFO @ Mon, 29 Jun 2020 23:57:19: 14000000 INFO @ Mon, 29 Jun 2020 23:57:22: 17000000 INFO @ Mon, 29 Jun 2020 23:57:22: 9000000 INFO @ Mon, 29 Jun 2020 23:57:26: 15000000 INFO @ Mon, 29 Jun 2020 23:57:27: #1 tag size is determined as 100 bps INFO @ Mon, 29 Jun 2020 23:57:27: #1 tag size = 100 INFO @ Mon, 29 Jun 2020 23:57:27: #1 total tags in treatment: 17646265 INFO @ Mon, 29 Jun 2020 23:57:27: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:57:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:57:27: #1 tags after filtering in treatment: 17646265 INFO @ Mon, 29 Jun 2020 23:57:27: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:57:27: #1 finished! INFO @ Mon, 29 Jun 2020 23:57:27: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:57:27: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:57:28: #2 number of paired peaks: 17 WARNING @ Mon, 29 Jun 2020 23:57:28: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:57:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX450797/SRX450797.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450797/SRX450797.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450797/SRX450797.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450797/SRX450797.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:57:30: 10000000 INFO @ Mon, 29 Jun 2020 23:57:33: 16000000 INFO @ Mon, 29 Jun 2020 23:57:37: 11000000 INFO @ Mon, 29 Jun 2020 23:57:41: 17000000 INFO @ Mon, 29 Jun 2020 23:57:44: 12000000 INFO @ Mon, 29 Jun 2020 23:57:46: #1 tag size is determined as 100 bps INFO @ Mon, 29 Jun 2020 23:57:46: #1 tag size = 100 INFO @ Mon, 29 Jun 2020 23:57:46: #1 total tags in treatment: 17646265 INFO @ Mon, 29 Jun 2020 23:57:46: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:57:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:57:46: #1 tags after filtering in treatment: 17646265 INFO @ Mon, 29 Jun 2020 23:57:46: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:57:46: #1 finished! INFO @ Mon, 29 Jun 2020 23:57:46: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:57:46: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:57:47: #2 number of paired peaks: 17 WARNING @ Mon, 29 Jun 2020 23:57:47: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:57:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX450797/SRX450797.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450797/SRX450797.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450797/SRX450797.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450797/SRX450797.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:57:52: 13000000 INFO @ Mon, 29 Jun 2020 23:57:59: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:58:06: 15000000 INFO @ Mon, 29 Jun 2020 23:58:14: 16000000 INFO @ Mon, 29 Jun 2020 23:58:22: 17000000 INFO @ Mon, 29 Jun 2020 23:58:27: #1 tag size is determined as 100 bps INFO @ Mon, 29 Jun 2020 23:58:27: #1 tag size = 100 INFO @ Mon, 29 Jun 2020 23:58:27: #1 total tags in treatment: 17646265 INFO @ Mon, 29 Jun 2020 23:58:27: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:58:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:58:27: #1 tags after filtering in treatment: 17646265 INFO @ Mon, 29 Jun 2020 23:58:27: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:58:27: #1 finished! INFO @ Mon, 29 Jun 2020 23:58:27: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:58:27: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:58:29: #2 number of paired peaks: 17 WARNING @ Mon, 29 Jun 2020 23:58:29: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:58:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX450797/SRX450797.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450797/SRX450797.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450797/SRX450797.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450797/SRX450797.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。