Job ID = 6528087 SRX = SRX450793 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:07:05 prefetch.2.10.7: 1) Downloading 'SRR1145610'... 2020-06-29T14:07:05 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:11:09 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:11:09 prefetch.2.10.7: 1) 'SRR1145610' was downloaded successfully Read 38801502 spots for SRR1145610/SRR1145610.sra Written 38801502 spots for SRR1145610/SRR1145610.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:56 38801502 reads; of these: 38801502 (100.00%) were unpaired; of these: 17227005 (44.40%) aligned 0 times 19092399 (49.21%) aligned exactly 1 time 2482098 (6.40%) aligned >1 times 55.60% overall alignment rate Time searching: 00:05:56 Overall time: 00:05:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 10413943 / 21574497 = 0.4827 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:33:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX450793/SRX450793.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX450793/SRX450793.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX450793/SRX450793.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX450793/SRX450793.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:33:47: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:33:47: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:33:53: 1000000 INFO @ Mon, 29 Jun 2020 23:33:59: 2000000 INFO @ Mon, 29 Jun 2020 23:34:05: 3000000 INFO @ Mon, 29 Jun 2020 23:34:10: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:34:16: 5000000 INFO @ Mon, 29 Jun 2020 23:34:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX450793/SRX450793.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX450793/SRX450793.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX450793/SRX450793.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX450793/SRX450793.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:34:17: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:34:17: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:34:23: 6000000 INFO @ Mon, 29 Jun 2020 23:34:24: 1000000 INFO @ Mon, 29 Jun 2020 23:34:29: 7000000 INFO @ Mon, 29 Jun 2020 23:34:30: 2000000 INFO @ Mon, 29 Jun 2020 23:34:35: 8000000 INFO @ Mon, 29 Jun 2020 23:34:37: 3000000 INFO @ Mon, 29 Jun 2020 23:34:41: 9000000 INFO @ Mon, 29 Jun 2020 23:34:43: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:34:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX450793/SRX450793.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX450793/SRX450793.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX450793/SRX450793.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX450793/SRX450793.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:34:47: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:34:47: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:34:47: 10000000 INFO @ Mon, 29 Jun 2020 23:34:49: 5000000 INFO @ Mon, 29 Jun 2020 23:34:54: 1000000 INFO @ Mon, 29 Jun 2020 23:34:54: 11000000 INFO @ Mon, 29 Jun 2020 23:34:55: #1 tag size is determined as 41 bps INFO @ Mon, 29 Jun 2020 23:34:55: #1 tag size = 41 INFO @ Mon, 29 Jun 2020 23:34:55: #1 total tags in treatment: 11160554 INFO @ Mon, 29 Jun 2020 23:34:55: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:34:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:34:55: #1 tags after filtering in treatment: 11160554 INFO @ Mon, 29 Jun 2020 23:34:55: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:34:55: #1 finished! INFO @ Mon, 29 Jun 2020 23:34:55: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:34:55: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:34:56: 6000000 INFO @ Mon, 29 Jun 2020 23:34:56: #2 number of paired peaks: 40 WARNING @ Mon, 29 Jun 2020 23:34:56: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:34:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX450793/SRX450793.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450793/SRX450793.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450793/SRX450793.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450793/SRX450793.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:35:00: 2000000 INFO @ Mon, 29 Jun 2020 23:35:02: 7000000 INFO @ Mon, 29 Jun 2020 23:35:06: 3000000 INFO @ Mon, 29 Jun 2020 23:35:08: 8000000 INFO @ Mon, 29 Jun 2020 23:35:13: 4000000 INFO @ Mon, 29 Jun 2020 23:35:14: 9000000 INFO @ Mon, 29 Jun 2020 23:35:19: 5000000 INFO @ Mon, 29 Jun 2020 23:35:20: 10000000 INFO @ Mon, 29 Jun 2020 23:35:25: 6000000 INFO @ Mon, 29 Jun 2020 23:35:27: 11000000 INFO @ Mon, 29 Jun 2020 23:35:28: #1 tag size is determined as 41 bps INFO @ Mon, 29 Jun 2020 23:35:28: #1 tag size = 41 INFO @ Mon, 29 Jun 2020 23:35:28: #1 total tags in treatment: 11160554 INFO @ Mon, 29 Jun 2020 23:35:28: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:35:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:35:28: #1 tags after filtering in treatment: 11160554 INFO @ Mon, 29 Jun 2020 23:35:28: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:35:28: #1 finished! INFO @ Mon, 29 Jun 2020 23:35:28: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:35:28: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:35:29: #2 number of paired peaks: 40 WARNING @ Mon, 29 Jun 2020 23:35:29: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:35:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX450793/SRX450793.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450793/SRX450793.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450793/SRX450793.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450793/SRX450793.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:35:31: 7000000 INFO @ Mon, 29 Jun 2020 23:35:37: 8000000 INFO @ Mon, 29 Jun 2020 23:35:42: 9000000 INFO @ Mon, 29 Jun 2020 23:35:48: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:35:54: 11000000 INFO @ Mon, 29 Jun 2020 23:35:55: #1 tag size is determined as 41 bps INFO @ Mon, 29 Jun 2020 23:35:55: #1 tag size = 41 INFO @ Mon, 29 Jun 2020 23:35:55: #1 total tags in treatment: 11160554 INFO @ Mon, 29 Jun 2020 23:35:55: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:35:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:35:55: #1 tags after filtering in treatment: 11160554 INFO @ Mon, 29 Jun 2020 23:35:55: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:35:55: #1 finished! INFO @ Mon, 29 Jun 2020 23:35:55: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:35:55: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:35:56: #2 number of paired peaks: 40 WARNING @ Mon, 29 Jun 2020 23:35:56: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:35:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX450793/SRX450793.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450793/SRX450793.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450793/SRX450793.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX450793/SRX450793.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。