Job ID = 6528084 SRX = SRX4375871 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:11:35 prefetch.2.10.7: 1) Downloading 'SRR7506525'... 2020-06-29T14:11:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:13:48 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:13:48 prefetch.2.10.7: 1) 'SRR7506525' was downloaded successfully Read 22261061 spots for SRR7506525/SRR7506525.sra Written 22261061 spots for SRR7506525/SRR7506525.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:39 22261061 reads; of these: 22261061 (100.00%) were unpaired; of these: 1835109 (8.24%) aligned 0 times 15536852 (69.79%) aligned exactly 1 time 4889100 (21.96%) aligned >1 times 91.76% overall alignment rate Time searching: 00:06:39 Overall time: 00:06:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3403720 / 20425952 = 0.1666 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:32:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4375871/SRX4375871.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4375871/SRX4375871.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4375871/SRX4375871.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4375871/SRX4375871.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:32:01: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:32:01: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:32:06: 1000000 INFO @ Mon, 29 Jun 2020 23:32:12: 2000000 INFO @ Mon, 29 Jun 2020 23:32:17: 3000000 INFO @ Mon, 29 Jun 2020 23:32:23: 4000000 INFO @ Mon, 29 Jun 2020 23:32:28: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:32:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4375871/SRX4375871.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4375871/SRX4375871.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4375871/SRX4375871.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4375871/SRX4375871.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:32:31: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:32:31: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:32:34: 6000000 INFO @ Mon, 29 Jun 2020 23:32:37: 1000000 INFO @ Mon, 29 Jun 2020 23:32:40: 7000000 INFO @ Mon, 29 Jun 2020 23:32:43: 2000000 INFO @ Mon, 29 Jun 2020 23:32:46: 8000000 INFO @ Mon, 29 Jun 2020 23:32:49: 3000000 INFO @ Mon, 29 Jun 2020 23:32:52: 9000000 INFO @ Mon, 29 Jun 2020 23:32:56: 4000000 INFO @ Mon, 29 Jun 2020 23:32:58: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:33:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4375871/SRX4375871.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4375871/SRX4375871.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4375871/SRX4375871.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4375871/SRX4375871.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:33:01: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:33:01: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:33:02: 5000000 INFO @ Mon, 29 Jun 2020 23:33:04: 11000000 INFO @ Mon, 29 Jun 2020 23:33:07: 1000000 INFO @ Mon, 29 Jun 2020 23:33:08: 6000000 INFO @ Mon, 29 Jun 2020 23:33:10: 12000000 INFO @ Mon, 29 Jun 2020 23:33:13: 2000000 INFO @ Mon, 29 Jun 2020 23:33:14: 7000000 INFO @ Mon, 29 Jun 2020 23:33:17: 13000000 INFO @ Mon, 29 Jun 2020 23:33:19: 3000000 INFO @ Mon, 29 Jun 2020 23:33:20: 8000000 INFO @ Mon, 29 Jun 2020 23:33:23: 14000000 INFO @ Mon, 29 Jun 2020 23:33:25: 4000000 INFO @ Mon, 29 Jun 2020 23:33:26: 9000000 INFO @ Mon, 29 Jun 2020 23:33:29: 15000000 INFO @ Mon, 29 Jun 2020 23:33:31: 5000000 INFO @ Mon, 29 Jun 2020 23:33:33: 10000000 INFO @ Mon, 29 Jun 2020 23:33:35: 16000000 INFO @ Mon, 29 Jun 2020 23:33:37: 6000000 INFO @ Mon, 29 Jun 2020 23:33:39: 11000000 INFO @ Mon, 29 Jun 2020 23:33:41: 17000000 INFO @ Mon, 29 Jun 2020 23:33:41: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:33:41: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:33:41: #1 total tags in treatment: 17022232 INFO @ Mon, 29 Jun 2020 23:33:41: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:33:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:33:42: #1 tags after filtering in treatment: 17022232 INFO @ Mon, 29 Jun 2020 23:33:42: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:33:42: #1 finished! INFO @ Mon, 29 Jun 2020 23:33:42: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:33:42: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:33:43: 7000000 INFO @ Mon, 29 Jun 2020 23:33:43: #2 number of paired peaks: 87 WARNING @ Mon, 29 Jun 2020 23:33:43: Too few paired peaks (87) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:33:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4375871/SRX4375871.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4375871/SRX4375871.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4375871/SRX4375871.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4375871/SRX4375871.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:33:45: 12000000 INFO @ Mon, 29 Jun 2020 23:33:49: 8000000 INFO @ Mon, 29 Jun 2020 23:33:51: 13000000 INFO @ Mon, 29 Jun 2020 23:33:55: 9000000 INFO @ Mon, 29 Jun 2020 23:33:57: 14000000 INFO @ Mon, 29 Jun 2020 23:34:00: 10000000 INFO @ Mon, 29 Jun 2020 23:34:04: 15000000 INFO @ Mon, 29 Jun 2020 23:34:06: 11000000 INFO @ Mon, 29 Jun 2020 23:34:10: 16000000 INFO @ Mon, 29 Jun 2020 23:34:12: 12000000 INFO @ Mon, 29 Jun 2020 23:34:16: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:34:16: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:34:16: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:34:16: #1 total tags in treatment: 17022232 INFO @ Mon, 29 Jun 2020 23:34:16: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:34:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:34:16: #1 tags after filtering in treatment: 17022232 INFO @ Mon, 29 Jun 2020 23:34:16: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:34:16: #1 finished! INFO @ Mon, 29 Jun 2020 23:34:16: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:34:16: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:34:18: #2 number of paired peaks: 87 WARNING @ Mon, 29 Jun 2020 23:34:18: Too few paired peaks (87) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:34:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4375871/SRX4375871.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4375871/SRX4375871.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4375871/SRX4375871.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4375871/SRX4375871.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:34:18: 13000000 INFO @ Mon, 29 Jun 2020 23:34:24: 14000000 INFO @ Mon, 29 Jun 2020 23:34:30: 15000000 INFO @ Mon, 29 Jun 2020 23:34:35: 16000000 INFO @ Mon, 29 Jun 2020 23:34:41: 17000000 INFO @ Mon, 29 Jun 2020 23:34:41: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:34:41: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:34:41: #1 total tags in treatment: 17022232 INFO @ Mon, 29 Jun 2020 23:34:41: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:34:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:34:41: #1 tags after filtering in treatment: 17022232 INFO @ Mon, 29 Jun 2020 23:34:41: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:34:41: #1 finished! INFO @ Mon, 29 Jun 2020 23:34:41: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:34:41: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:34:43: #2 number of paired peaks: 87 WARNING @ Mon, 29 Jun 2020 23:34:43: Too few paired peaks (87) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:34:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4375871/SRX4375871.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4375871/SRX4375871.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4375871/SRX4375871.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4375871/SRX4375871.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。