Job ID = 11171359 sra ファイルのダウンロード中... Completed: 1029271K bytes transferred in 13 seconds (622636K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 72500328 spots for /home/okishinya/chipatlas/results/dm3/SRX4315034/SRR7444495.sra Written 72500328 spots for /home/okishinya/chipatlas/results/dm3/SRX4315034/SRR7444495.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:20:44 72500328 reads; of these: 72500328 (100.00%) were unpaired; of these: 8681 (0.01%) aligned 0 times 65330115 (90.11%) aligned exactly 1 time 7161532 (9.88%) aligned >1 times 99.99% overall alignment rate Time searching: 00:20:45 Overall time: 00:20:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 7165733 / 72491647 = 0.0988 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 08 Sep 2018 14:48:27: # Command line: callpeak -t SRX4315034.bam -f BAM -g dm -n SRX4315034.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4315034.20 # format = BAM # ChIP-seq file = ['SRX4315034.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 14:48:27: #1 read tag files... INFO @ Sat, 08 Sep 2018 14:48:27: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 14:48:27: # Command line: callpeak -t SRX4315034.bam -f BAM -g dm -n SRX4315034.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4315034.05 # format = BAM # ChIP-seq file = ['SRX4315034.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 14:48:27: #1 read tag files... INFO @ Sat, 08 Sep 2018 14:48:27: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 14:48:27: # Command line: callpeak -t SRX4315034.bam -f BAM -g dm -n SRX4315034.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4315034.10 # format = BAM # ChIP-seq file = ['SRX4315034.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 08 Sep 2018 14:48:27: #1 read tag files... INFO @ Sat, 08 Sep 2018 14:48:27: #1 read treatment tags... INFO @ Sat, 08 Sep 2018 14:48:34: 1000000 INFO @ Sat, 08 Sep 2018 14:48:34: 1000000 INFO @ Sat, 08 Sep 2018 14:48:34: 1000000 INFO @ Sat, 08 Sep 2018 14:48:40: 2000000 INFO @ Sat, 08 Sep 2018 14:48:40: 2000000 INFO @ Sat, 08 Sep 2018 14:48:41: 2000000 INFO @ Sat, 08 Sep 2018 14:48:47: 3000000 INFO @ Sat, 08 Sep 2018 14:48:47: 3000000 INFO @ Sat, 08 Sep 2018 14:48:48: 3000000 INFO @ Sat, 08 Sep 2018 14:48:53: 4000000 INFO @ Sat, 08 Sep 2018 14:48:54: 4000000 INFO @ Sat, 08 Sep 2018 14:48:54: 4000000 INFO @ Sat, 08 Sep 2018 14:49:00: 5000000 INFO @ Sat, 08 Sep 2018 14:49:01: 5000000 INFO @ Sat, 08 Sep 2018 14:49:01: 5000000 INFO @ Sat, 08 Sep 2018 14:49:07: 6000000 INFO @ Sat, 08 Sep 2018 14:49:07: 6000000 INFO @ Sat, 08 Sep 2018 14:49:08: 6000000 INFO @ Sat, 08 Sep 2018 14:49:13: 7000000 INFO @ Sat, 08 Sep 2018 14:49:14: 7000000 INFO @ Sat, 08 Sep 2018 14:49:15: 7000000 INFO @ Sat, 08 Sep 2018 14:49:20: 8000000 INFO @ Sat, 08 Sep 2018 14:49:21: 8000000 INFO @ Sat, 08 Sep 2018 14:49:22: 8000000 INFO @ Sat, 08 Sep 2018 14:49:27: 9000000 INFO @ Sat, 08 Sep 2018 14:49:28: 9000000 INFO @ Sat, 08 Sep 2018 14:49:28: 9000000 INFO @ Sat, 08 Sep 2018 14:49:33: 10000000 INFO @ Sat, 08 Sep 2018 14:49:35: 10000000 INFO @ Sat, 08 Sep 2018 14:49:35: 10000000 INFO @ Sat, 08 Sep 2018 14:49:40: 11000000 INFO @ Sat, 08 Sep 2018 14:49:41: 11000000 INFO @ Sat, 08 Sep 2018 14:49:42: 11000000 INFO @ Sat, 08 Sep 2018 14:49:47: 12000000 INFO @ Sat, 08 Sep 2018 14:49:48: 12000000 INFO @ Sat, 08 Sep 2018 14:49:49: 12000000 INFO @ Sat, 08 Sep 2018 14:49:53: 13000000 INFO @ Sat, 08 Sep 2018 14:49:55: 13000000 INFO @ Sat, 08 Sep 2018 14:49:56: 13000000 INFO @ Sat, 08 Sep 2018 14:50:00: 14000000 INFO @ Sat, 08 Sep 2018 14:50:02: 14000000 INFO @ Sat, 08 Sep 2018 14:50:02: 14000000 INFO @ Sat, 08 Sep 2018 14:50:07: 15000000 INFO @ Sat, 08 Sep 2018 14:50:09: 15000000 INFO @ Sat, 08 Sep 2018 14:50:09: 15000000 INFO @ Sat, 08 Sep 2018 14:50:13: 16000000 INFO @ Sat, 08 Sep 2018 14:50:16: 16000000 INFO @ Sat, 08 Sep 2018 14:50:16: 16000000 INFO @ Sat, 08 Sep 2018 14:50:20: 17000000 INFO @ Sat, 08 Sep 2018 14:50:23: 17000000 INFO @ Sat, 08 Sep 2018 14:50:23: 17000000 INFO @ Sat, 08 Sep 2018 14:50:27: 18000000 INFO @ Sat, 08 Sep 2018 14:50:30: 18000000 INFO @ Sat, 08 Sep 2018 14:50:30: 18000000 INFO @ Sat, 08 Sep 2018 14:50:33: 19000000 INFO @ Sat, 08 Sep 2018 14:50:37: 19000000 INFO @ Sat, 08 Sep 2018 14:50:37: 19000000 INFO @ Sat, 08 Sep 2018 14:50:40: 20000000 INFO @ Sat, 08 Sep 2018 14:50:43: 20000000 INFO @ Sat, 08 Sep 2018 14:50:44: 20000000 INFO @ Sat, 08 Sep 2018 14:50:47: 21000000 INFO @ Sat, 08 Sep 2018 14:50:50: 21000000 INFO @ Sat, 08 Sep 2018 14:50:51: 21000000 INFO @ Sat, 08 Sep 2018 14:50:54: 22000000 INFO @ Sat, 08 Sep 2018 14:50:57: 22000000 INFO @ Sat, 08 Sep 2018 14:50:58: 22000000 INFO @ Sat, 08 Sep 2018 14:51:00: 23000000 INFO @ Sat, 08 Sep 2018 14:51:04: 23000000 INFO @ Sat, 08 Sep 2018 14:51:04: 23000000 INFO @ Sat, 08 Sep 2018 14:51:07: 24000000 INFO @ Sat, 08 Sep 2018 14:51:11: 24000000 INFO @ Sat, 08 Sep 2018 14:51:11: 24000000 INFO @ Sat, 08 Sep 2018 14:51:14: 25000000 INFO @ Sat, 08 Sep 2018 14:51:18: 25000000 INFO @ Sat, 08 Sep 2018 14:51:18: 25000000 INFO @ Sat, 08 Sep 2018 14:51:20: 26000000 INFO @ Sat, 08 Sep 2018 14:51:25: 26000000 INFO @ Sat, 08 Sep 2018 14:51:25: 26000000 INFO @ Sat, 08 Sep 2018 14:51:27: 27000000 INFO @ Sat, 08 Sep 2018 14:51:32: 27000000 INFO @ Sat, 08 Sep 2018 14:51:32: 27000000 INFO @ Sat, 08 Sep 2018 14:51:34: 28000000 INFO @ Sat, 08 Sep 2018 14:51:39: 28000000 INFO @ Sat, 08 Sep 2018 14:51:39: 28000000 INFO @ Sat, 08 Sep 2018 14:51:40: 29000000 INFO @ Sat, 08 Sep 2018 14:51:45: 29000000 INFO @ Sat, 08 Sep 2018 14:51:46: 29000000 INFO @ Sat, 08 Sep 2018 14:51:47: 30000000 INFO @ Sat, 08 Sep 2018 14:51:52: 30000000 INFO @ Sat, 08 Sep 2018 14:51:52: 30000000 INFO @ Sat, 08 Sep 2018 14:51:53: 31000000 INFO @ Sat, 08 Sep 2018 14:51:59: 31000000 INFO @ Sat, 08 Sep 2018 14:51:59: 31000000 INFO @ Sat, 08 Sep 2018 14:52:00: 32000000 INFO @ Sat, 08 Sep 2018 14:52:06: 32000000 INFO @ Sat, 08 Sep 2018 14:52:06: 32000000 INFO @ Sat, 08 Sep 2018 14:52:07: 33000000 INFO @ Sat, 08 Sep 2018 14:52:13: 33000000 INFO @ Sat, 08 Sep 2018 14:52:13: 33000000 INFO @ Sat, 08 Sep 2018 14:52:14: 34000000 INFO @ Sat, 08 Sep 2018 14:52:20: 34000000 INFO @ Sat, 08 Sep 2018 14:52:20: 34000000 INFO @ Sat, 08 Sep 2018 14:52:21: 35000000 INFO @ Sat, 08 Sep 2018 14:52:27: 35000000 INFO @ Sat, 08 Sep 2018 14:52:27: 35000000 INFO @ Sat, 08 Sep 2018 14:52:28: 36000000 INFO @ Sat, 08 Sep 2018 14:52:34: 36000000 INFO @ Sat, 08 Sep 2018 14:52:34: 36000000 INFO @ Sat, 08 Sep 2018 14:52:34: 37000000 INFO @ Sat, 08 Sep 2018 14:52:41: 37000000 INFO @ Sat, 08 Sep 2018 14:52:41: 37000000 INFO @ Sat, 08 Sep 2018 14:52:41: 38000000 INFO @ Sat, 08 Sep 2018 14:52:47: 38000000 INFO @ Sat, 08 Sep 2018 14:52:48: 39000000 INFO @ Sat, 08 Sep 2018 14:52:48: 38000000 INFO @ Sat, 08 Sep 2018 14:52:54: 39000000 INFO @ Sat, 08 Sep 2018 14:52:55: 40000000 INFO @ Sat, 08 Sep 2018 14:52:55: 39000000 INFO @ Sat, 08 Sep 2018 14:53:01: 40000000 INFO @ Sat, 08 Sep 2018 14:53:01: 41000000 INFO @ Sat, 08 Sep 2018 14:53:02: 40000000 INFO @ Sat, 08 Sep 2018 14:53:08: 41000000 INFO @ Sat, 08 Sep 2018 14:53:08: 42000000 INFO @ Sat, 08 Sep 2018 14:53:09: 41000000 INFO @ Sat, 08 Sep 2018 14:53:14: 42000000 INFO @ Sat, 08 Sep 2018 14:53:15: 43000000 INFO @ Sat, 08 Sep 2018 14:53:16: 42000000 INFO @ Sat, 08 Sep 2018 14:53:21: 43000000 INFO @ Sat, 08 Sep 2018 14:53:22: 44000000 INFO @ Sat, 08 Sep 2018 14:53:23: 43000000 INFO @ Sat, 08 Sep 2018 14:53:28: 44000000 INFO @ Sat, 08 Sep 2018 14:53:29: 45000000 INFO @ Sat, 08 Sep 2018 14:53:30: 44000000 INFO @ Sat, 08 Sep 2018 14:53:35: 45000000 INFO @ Sat, 08 Sep 2018 14:53:35: 46000000 INFO @ Sat, 08 Sep 2018 14:53:37: 45000000 INFO @ Sat, 08 Sep 2018 14:53:41: 46000000 INFO @ Sat, 08 Sep 2018 14:53:42: 47000000 INFO @ Sat, 08 Sep 2018 14:53:44: 46000000 INFO @ Sat, 08 Sep 2018 14:53:48: 47000000 INFO @ Sat, 08 Sep 2018 14:53:49: 48000000 INFO @ Sat, 08 Sep 2018 14:53:51: 47000000 INFO @ Sat, 08 Sep 2018 14:53:55: 48000000 INFO @ Sat, 08 Sep 2018 14:53:56: 49000000 INFO @ Sat, 08 Sep 2018 14:53:57: 48000000 INFO @ Sat, 08 Sep 2018 14:54:01: 49000000 INFO @ Sat, 08 Sep 2018 14:54:03: 50000000 INFO @ Sat, 08 Sep 2018 14:54:04: 49000000 INFO @ Sat, 08 Sep 2018 14:54:08: 50000000 INFO @ Sat, 08 Sep 2018 14:54:09: 51000000 INFO @ Sat, 08 Sep 2018 14:54:11: 50000000 INFO @ Sat, 08 Sep 2018 14:54:15: 51000000 INFO @ Sat, 08 Sep 2018 14:54:16: 52000000 INFO @ Sat, 08 Sep 2018 14:54:18: 51000000 INFO @ Sat, 08 Sep 2018 14:54:22: 52000000 INFO @ Sat, 08 Sep 2018 14:54:23: 53000000 INFO @ Sat, 08 Sep 2018 14:54:25: 52000000 INFO @ Sat, 08 Sep 2018 14:54:29: 53000000 INFO @ Sat, 08 Sep 2018 14:54:30: 54000000 INFO @ Sat, 08 Sep 2018 14:54:33: 53000000 INFO @ Sat, 08 Sep 2018 14:54:36: 54000000 INFO @ Sat, 08 Sep 2018 14:54:37: 55000000 INFO @ Sat, 08 Sep 2018 14:54:40: 54000000 INFO @ Sat, 08 Sep 2018 14:54:42: 55000000 INFO @ Sat, 08 Sep 2018 14:54:44: 56000000 INFO @ Sat, 08 Sep 2018 14:54:47: 55000000 INFO @ Sat, 08 Sep 2018 14:54:49: 56000000 INFO @ Sat, 08 Sep 2018 14:54:50: 57000000 INFO @ Sat, 08 Sep 2018 14:54:53: 56000000 INFO @ Sat, 08 Sep 2018 14:54:56: 57000000 INFO @ Sat, 08 Sep 2018 14:54:57: 58000000 INFO @ Sat, 08 Sep 2018 14:55:00: 57000000 INFO @ Sat, 08 Sep 2018 14:55:02: 58000000 INFO @ Sat, 08 Sep 2018 14:55:04: 59000000 INFO @ Sat, 08 Sep 2018 14:55:07: 58000000 INFO @ Sat, 08 Sep 2018 14:55:09: 59000000 INFO @ Sat, 08 Sep 2018 14:55:11: 60000000 INFO @ Sat, 08 Sep 2018 14:55:14: 59000000 INFO @ Sat, 08 Sep 2018 14:55:16: 60000000 INFO @ Sat, 08 Sep 2018 14:55:17: 61000000 INFO @ Sat, 08 Sep 2018 14:55:21: 60000000 INFO @ Sat, 08 Sep 2018 14:55:22: 61000000 INFO @ Sat, 08 Sep 2018 14:55:24: 62000000 INFO @ Sat, 08 Sep 2018 14:55:28: 61000000 INFO @ Sat, 08 Sep 2018 14:55:29: 62000000 INFO @ Sat, 08 Sep 2018 14:55:31: 63000000 INFO @ Sat, 08 Sep 2018 14:55:35: 62000000 INFO @ Sat, 08 Sep 2018 14:55:36: 63000000 INFO @ Sat, 08 Sep 2018 14:55:37: 64000000 INFO @ Sat, 08 Sep 2018 14:55:42: 63000000 INFO @ Sat, 08 Sep 2018 14:55:43: 64000000 INFO @ Sat, 08 Sep 2018 14:55:43: 65000000 INFO @ Sat, 08 Sep 2018 14:55:46: #1 tag size is determined as 73 bps INFO @ Sat, 08 Sep 2018 14:55:46: #1 tag size = 73 INFO @ Sat, 08 Sep 2018 14:55:46: #1 total tags in treatment: 65325914 INFO @ Sat, 08 Sep 2018 14:55:46: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 14:55:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 14:55:47: #1 tags after filtering in treatment: 65325914 INFO @ Sat, 08 Sep 2018 14:55:47: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 14:55:47: #1 finished! INFO @ Sat, 08 Sep 2018 14:55:47: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 14:55:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 14:55:50: 64000000 INFO @ Sat, 08 Sep 2018 14:55:50: 65000000 INFO @ Sat, 08 Sep 2018 14:55:52: #2 number of paired peaks: 0 WARNING @ Sat, 08 Sep 2018 14:55:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 14:55:52: Process for pairing-model is terminated! cat: SRX4315034.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 10 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4315034.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4315034.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4315034.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 14:55:53: #1 tag size is determined as 73 bps INFO @ Sat, 08 Sep 2018 14:55:53: #1 tag size = 73 INFO @ Sat, 08 Sep 2018 14:55:53: #1 total tags in treatment: 65325914 INFO @ Sat, 08 Sep 2018 14:55:53: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 14:55:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 14:55:54: #1 tags after filtering in treatment: 65325914 INFO @ Sat, 08 Sep 2018 14:55:54: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 14:55:54: #1 finished! INFO @ Sat, 08 Sep 2018 14:55:54: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 14:55:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 14:55:56: 65000000 INFO @ Sat, 08 Sep 2018 14:55:59: #1 tag size is determined as 73 bps INFO @ Sat, 08 Sep 2018 14:55:59: #1 tag size = 73 INFO @ Sat, 08 Sep 2018 14:55:59: #1 total tags in treatment: 65325914 INFO @ Sat, 08 Sep 2018 14:55:59: #1 user defined the maximum tags... INFO @ Sat, 08 Sep 2018 14:55:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 08 Sep 2018 14:55:59: #2 number of paired peaks: 0 WARNING @ Sat, 08 Sep 2018 14:55:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 14:55:59: Process for pairing-model is terminated! cat: SRX4315034.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4315034.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4315034.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4315034.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 08 Sep 2018 14:56:00: #1 tags after filtering in treatment: 65325914 INFO @ Sat, 08 Sep 2018 14:56:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 08 Sep 2018 14:56:00: #1 finished! INFO @ Sat, 08 Sep 2018 14:56:00: #2 Build Peak Model... INFO @ Sat, 08 Sep 2018 14:56:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 08 Sep 2018 14:56:04: #2 number of paired peaks: 0 WARNING @ Sat, 08 Sep 2018 14:56:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 08 Sep 2018 14:56:04: Process for pairing-model is terminated! cat: SRX4315034.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4315034.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4315034.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4315034.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。