Job ID = 5720725 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 32,671,823 reads read : 32,671,823 reads written : 32,671,823 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:44 32671823 reads; of these: 32671823 (100.00%) were unpaired; of these: 1213664 (3.71%) aligned 0 times 27535644 (84.28%) aligned exactly 1 time 3922515 (12.01%) aligned >1 times 96.29% overall alignment rate Time searching: 00:07:44 Overall time: 00:07:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 9439209 / 31458159 = 0.3001 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 01:28:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4158197/SRX4158197.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4158197/SRX4158197.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4158197/SRX4158197.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4158197/SRX4158197.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 01:28:28: #1 read tag files... INFO @ Thu, 16 Apr 2020 01:28:28: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 01:28:34: 1000000 INFO @ Thu, 16 Apr 2020 01:28:39: 2000000 INFO @ Thu, 16 Apr 2020 01:28:44: 3000000 INFO @ Thu, 16 Apr 2020 01:28:50: 4000000 INFO @ Thu, 16 Apr 2020 01:28:55: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 01:28:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4158197/SRX4158197.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4158197/SRX4158197.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4158197/SRX4158197.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4158197/SRX4158197.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 01:28:58: #1 read tag files... INFO @ Thu, 16 Apr 2020 01:28:58: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 01:29:01: 6000000 INFO @ Thu, 16 Apr 2020 01:29:04: 1000000 INFO @ Thu, 16 Apr 2020 01:29:07: 7000000 INFO @ Thu, 16 Apr 2020 01:29:10: 2000000 INFO @ Thu, 16 Apr 2020 01:29:13: 8000000 INFO @ Thu, 16 Apr 2020 01:29:16: 3000000 INFO @ Thu, 16 Apr 2020 01:29:19: 9000000 INFO @ Thu, 16 Apr 2020 01:29:22: 4000000 INFO @ Thu, 16 Apr 2020 01:29:24: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 01:29:28: 5000000 INFO @ Thu, 16 Apr 2020 01:29:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX4158197/SRX4158197.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX4158197/SRX4158197.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX4158197/SRX4158197.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX4158197/SRX4158197.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 01:29:28: #1 read tag files... INFO @ Thu, 16 Apr 2020 01:29:28: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 01:29:30: 11000000 INFO @ Thu, 16 Apr 2020 01:29:34: 6000000 INFO @ Thu, 16 Apr 2020 01:29:34: 1000000 INFO @ Thu, 16 Apr 2020 01:29:36: 12000000 INFO @ Thu, 16 Apr 2020 01:29:40: 7000000 INFO @ Thu, 16 Apr 2020 01:29:40: 2000000 INFO @ Thu, 16 Apr 2020 01:29:42: 13000000 INFO @ Thu, 16 Apr 2020 01:29:46: 8000000 INFO @ Thu, 16 Apr 2020 01:29:46: 3000000 INFO @ Thu, 16 Apr 2020 01:29:48: 14000000 INFO @ Thu, 16 Apr 2020 01:29:52: 9000000 INFO @ Thu, 16 Apr 2020 01:29:52: 4000000 INFO @ Thu, 16 Apr 2020 01:29:54: 15000000 INFO @ Thu, 16 Apr 2020 01:29:58: 10000000 INFO @ Thu, 16 Apr 2020 01:29:58: 5000000 INFO @ Thu, 16 Apr 2020 01:29:59: 16000000 INFO @ Thu, 16 Apr 2020 01:30:04: 11000000 INFO @ Thu, 16 Apr 2020 01:30:04: 6000000 INFO @ Thu, 16 Apr 2020 01:30:05: 17000000 INFO @ Thu, 16 Apr 2020 01:30:10: 12000000 INFO @ Thu, 16 Apr 2020 01:30:10: 7000000 INFO @ Thu, 16 Apr 2020 01:30:11: 18000000 INFO @ Thu, 16 Apr 2020 01:30:15: 13000000 INFO @ Thu, 16 Apr 2020 01:30:16: 8000000 INFO @ Thu, 16 Apr 2020 01:30:17: 19000000 INFO @ Thu, 16 Apr 2020 01:30:21: 14000000 INFO @ Thu, 16 Apr 2020 01:30:22: 9000000 INFO @ Thu, 16 Apr 2020 01:30:23: 20000000 INFO @ Thu, 16 Apr 2020 01:30:27: 15000000 INFO @ Thu, 16 Apr 2020 01:30:28: 10000000 INFO @ Thu, 16 Apr 2020 01:30:29: 21000000 INFO @ Thu, 16 Apr 2020 01:30:33: 16000000 INFO @ Thu, 16 Apr 2020 01:30:34: 11000000 INFO @ Thu, 16 Apr 2020 01:30:35: 22000000 INFO @ Thu, 16 Apr 2020 01:30:35: #1 tag size is determined as 50 bps INFO @ Thu, 16 Apr 2020 01:30:35: #1 tag size = 50 INFO @ Thu, 16 Apr 2020 01:30:35: #1 total tags in treatment: 22018950 INFO @ Thu, 16 Apr 2020 01:30:35: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 01:30:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 01:30:36: #1 tags after filtering in treatment: 22018950 INFO @ Thu, 16 Apr 2020 01:30:36: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 16 Apr 2020 01:30:36: #1 finished! INFO @ Thu, 16 Apr 2020 01:30:36: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 01:30:36: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 01:30:37: #2 number of paired peaks: 75 WARNING @ Thu, 16 Apr 2020 01:30:37: Too few paired peaks (75) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 16 Apr 2020 01:30:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4158197/SRX4158197.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4158197/SRX4158197.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4158197/SRX4158197.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4158197/SRX4158197.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 01:30:39: 17000000 INFO @ Thu, 16 Apr 2020 01:30:40: 12000000 INFO @ Thu, 16 Apr 2020 01:30:45: 18000000 INFO @ Thu, 16 Apr 2020 01:30:45: 13000000 INFO @ Thu, 16 Apr 2020 01:30:51: 19000000 INFO @ Thu, 16 Apr 2020 01:30:51: 14000000 INFO @ Thu, 16 Apr 2020 01:30:57: 20000000 INFO @ Thu, 16 Apr 2020 01:30:57: 15000000 INFO @ Thu, 16 Apr 2020 01:31:03: 21000000 INFO @ Thu, 16 Apr 2020 01:31:03: 16000000 INFO @ Thu, 16 Apr 2020 01:31:08: 22000000 INFO @ Thu, 16 Apr 2020 01:31:08: 17000000 INFO @ Thu, 16 Apr 2020 01:31:09: #1 tag size is determined as 50 bps INFO @ Thu, 16 Apr 2020 01:31:09: #1 tag size = 50 INFO @ Thu, 16 Apr 2020 01:31:09: #1 total tags in treatment: 22018950 INFO @ Thu, 16 Apr 2020 01:31:09: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 01:31:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 01:31:09: #1 tags after filtering in treatment: 22018950 INFO @ Thu, 16 Apr 2020 01:31:09: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 16 Apr 2020 01:31:09: #1 finished! INFO @ Thu, 16 Apr 2020 01:31:09: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 01:31:09: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 01:31:10: #2 number of paired peaks: 75 WARNING @ Thu, 16 Apr 2020 01:31:10: Too few paired peaks (75) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 16 Apr 2020 01:31:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4158197/SRX4158197.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4158197/SRX4158197.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4158197/SRX4158197.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4158197/SRX4158197.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 01:31:14: 18000000 INFO @ Thu, 16 Apr 2020 01:31:20: 19000000 INFO @ Thu, 16 Apr 2020 01:31:25: 20000000 INFO @ Thu, 16 Apr 2020 01:31:31: 21000000 INFO @ Thu, 16 Apr 2020 01:31:37: 22000000 INFO @ Thu, 16 Apr 2020 01:31:37: #1 tag size is determined as 50 bps INFO @ Thu, 16 Apr 2020 01:31:37: #1 tag size = 50 INFO @ Thu, 16 Apr 2020 01:31:37: #1 total tags in treatment: 22018950 INFO @ Thu, 16 Apr 2020 01:31:37: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 01:31:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 01:31:37: #1 tags after filtering in treatment: 22018950 INFO @ Thu, 16 Apr 2020 01:31:37: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 16 Apr 2020 01:31:37: #1 finished! INFO @ Thu, 16 Apr 2020 01:31:37: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 01:31:37: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 01:31:38: #2 number of paired peaks: 75 WARNING @ Thu, 16 Apr 2020 01:31:38: Too few paired peaks (75) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 16 Apr 2020 01:31:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX4158197/SRX4158197.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4158197/SRX4158197.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4158197/SRX4158197.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX4158197/SRX4158197.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。