Job ID = 10714554 sra ファイルのダウンロード中... Completed: 1718913K bytes transferred in 132 seconds (106659K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 89331692 spots for /home/okishinya/chipatlas/results/dm3/SRX4047177/SRR7126158.sra Written 89331692 spots for /home/okishinya/chipatlas/results/dm3/SRX4047177/SRR7126158.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:38:38 89331692 reads; of these: 89331692 (100.00%) were unpaired; of these: 4557442 (5.10%) aligned 0 times 61691128 (69.06%) aligned exactly 1 time 23083122 (25.84%) aligned >1 times 94.90% overall alignment rate Time searching: 00:38:38 Overall time: 00:38:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 36 files... [bam_rmdupse_core] 37547508 / 84774250 = 0.4429 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 03 Jun 2018 14:24:06: # Command line: callpeak -t SRX4047177.bam -f BAM -g dm -n SRX4047177.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4047177.20 # format = BAM # ChIP-seq file = ['SRX4047177.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 03 Jun 2018 14:24:06: #1 read tag files... INFO @ Sun, 03 Jun 2018 14:24:06: #1 read treatment tags... INFO @ Sun, 03 Jun 2018 14:24:06: # Command line: callpeak -t SRX4047177.bam -f BAM -g dm -n SRX4047177.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4047177.10 # format = BAM # ChIP-seq file = ['SRX4047177.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 03 Jun 2018 14:24:06: #1 read tag files... INFO @ Sun, 03 Jun 2018 14:24:06: #1 read treatment tags... INFO @ Sun, 03 Jun 2018 14:24:06: # Command line: callpeak -t SRX4047177.bam -f BAM -g dm -n SRX4047177.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4047177.05 # format = BAM # ChIP-seq file = ['SRX4047177.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 03 Jun 2018 14:24:06: #1 read tag files... INFO @ Sun, 03 Jun 2018 14:24:06: #1 read treatment tags... INFO @ Sun, 03 Jun 2018 14:24:14: 1000000 INFO @ Sun, 03 Jun 2018 14:24:14: 1000000 INFO @ Sun, 03 Jun 2018 14:24:14: 1000000 INFO @ Sun, 03 Jun 2018 14:24:21: 2000000 INFO @ Sun, 03 Jun 2018 14:24:21: 2000000 INFO @ Sun, 03 Jun 2018 14:24:21: 2000000 INFO @ Sun, 03 Jun 2018 14:24:29: 3000000 INFO @ Sun, 03 Jun 2018 14:24:29: 3000000 INFO @ Sun, 03 Jun 2018 14:24:29: 3000000 INFO @ Sun, 03 Jun 2018 14:24:36: 4000000 INFO @ Sun, 03 Jun 2018 14:24:36: 4000000 INFO @ Sun, 03 Jun 2018 14:24:36: 4000000 INFO @ Sun, 03 Jun 2018 14:24:43: 5000000 INFO @ Sun, 03 Jun 2018 14:24:43: 5000000 INFO @ Sun, 03 Jun 2018 14:24:43: 5000000 INFO @ Sun, 03 Jun 2018 14:24:50: 6000000 INFO @ Sun, 03 Jun 2018 14:24:50: 6000000 INFO @ Sun, 03 Jun 2018 14:24:50: 6000000 INFO @ Sun, 03 Jun 2018 14:24:56: 7000000 INFO @ Sun, 03 Jun 2018 14:24:56: 7000000 INFO @ Sun, 03 Jun 2018 14:24:56: 7000000 INFO @ Sun, 03 Jun 2018 14:25:03: 8000000 INFO @ Sun, 03 Jun 2018 14:25:03: 8000000 INFO @ Sun, 03 Jun 2018 14:25:03: 8000000 INFO @ Sun, 03 Jun 2018 14:25:09: 9000000 INFO @ Sun, 03 Jun 2018 14:25:09: 9000000 INFO @ Sun, 03 Jun 2018 14:25:09: 9000000 INFO @ Sun, 03 Jun 2018 14:25:16: 10000000 INFO @ Sun, 03 Jun 2018 14:25:16: 10000000 INFO @ Sun, 03 Jun 2018 14:25:16: 10000000 INFO @ Sun, 03 Jun 2018 14:25:22: 11000000 INFO @ Sun, 03 Jun 2018 14:25:22: 11000000 INFO @ Sun, 03 Jun 2018 14:25:23: 11000000 INFO @ Sun, 03 Jun 2018 14:25:29: 12000000 INFO @ Sun, 03 Jun 2018 14:25:29: 12000000 INFO @ Sun, 03 Jun 2018 14:25:29: 12000000 INFO @ Sun, 03 Jun 2018 14:25:35: 13000000 INFO @ Sun, 03 Jun 2018 14:25:35: 13000000 INFO @ Sun, 03 Jun 2018 14:25:36: 13000000 INFO @ Sun, 03 Jun 2018 14:25:41: 14000000 INFO @ Sun, 03 Jun 2018 14:25:41: 14000000 INFO @ Sun, 03 Jun 2018 14:25:42: 14000000 INFO @ Sun, 03 Jun 2018 14:25:47: 15000000 INFO @ Sun, 03 Jun 2018 14:25:47: 15000000 INFO @ Sun, 03 Jun 2018 14:25:48: 15000000 INFO @ Sun, 03 Jun 2018 14:25:53: 16000000 INFO @ Sun, 03 Jun 2018 14:25:54: 16000000 INFO @ Sun, 03 Jun 2018 14:25:54: 16000000 INFO @ Sun, 03 Jun 2018 14:26:00: 17000000 INFO @ Sun, 03 Jun 2018 14:26:00: 17000000 INFO @ Sun, 03 Jun 2018 14:26:00: 17000000 INFO @ Sun, 03 Jun 2018 14:26:06: 18000000 INFO @ Sun, 03 Jun 2018 14:26:06: 18000000 INFO @ Sun, 03 Jun 2018 14:26:06: 18000000 INFO @ Sun, 03 Jun 2018 14:26:11: 19000000 INFO @ Sun, 03 Jun 2018 14:26:12: 19000000 INFO @ Sun, 03 Jun 2018 14:26:13: 19000000 INFO @ Sun, 03 Jun 2018 14:26:17: 20000000 INFO @ Sun, 03 Jun 2018 14:26:18: 20000000 INFO @ Sun, 03 Jun 2018 14:26:19: 20000000 INFO @ Sun, 03 Jun 2018 14:26:23: 21000000 INFO @ Sun, 03 Jun 2018 14:26:25: 21000000 INFO @ Sun, 03 Jun 2018 14:26:26: 21000000 INFO @ Sun, 03 Jun 2018 14:26:29: 22000000 INFO @ Sun, 03 Jun 2018 14:26:31: 22000000 INFO @ Sun, 03 Jun 2018 14:26:32: 22000000 INFO @ Sun, 03 Jun 2018 14:26:35: 23000000 INFO @ Sun, 03 Jun 2018 14:26:38: 23000000 INFO @ Sun, 03 Jun 2018 14:26:39: 23000000 INFO @ Sun, 03 Jun 2018 14:26:41: 24000000 INFO @ Sun, 03 Jun 2018 14:26:44: 24000000 INFO @ Sun, 03 Jun 2018 14:26:45: 24000000 INFO @ Sun, 03 Jun 2018 14:26:47: 25000000 INFO @ Sun, 03 Jun 2018 14:26:50: 25000000 INFO @ Sun, 03 Jun 2018 14:26:52: 25000000 INFO @ Sun, 03 Jun 2018 14:26:53: 26000000 INFO @ Sun, 03 Jun 2018 14:26:57: 26000000 INFO @ Sun, 03 Jun 2018 14:26:58: 26000000 INFO @ Sun, 03 Jun 2018 14:26:59: 27000000 INFO @ Sun, 03 Jun 2018 14:27:03: 27000000 INFO @ Sun, 03 Jun 2018 14:27:04: 28000000 INFO @ Sun, 03 Jun 2018 14:27:05: 27000000 INFO @ Sun, 03 Jun 2018 14:27:09: 28000000 INFO @ Sun, 03 Jun 2018 14:27:10: 29000000 INFO @ Sun, 03 Jun 2018 14:27:11: 28000000 INFO @ Sun, 03 Jun 2018 14:27:16: 29000000 INFO @ Sun, 03 Jun 2018 14:27:16: 30000000 INFO @ Sun, 03 Jun 2018 14:27:18: 29000000 INFO @ Sun, 03 Jun 2018 14:27:22: 31000000 INFO @ Sun, 03 Jun 2018 14:27:22: 30000000 INFO @ Sun, 03 Jun 2018 14:27:24: 30000000 INFO @ Sun, 03 Jun 2018 14:27:28: 32000000 INFO @ Sun, 03 Jun 2018 14:27:29: 31000000 INFO @ Sun, 03 Jun 2018 14:27:31: 31000000 INFO @ Sun, 03 Jun 2018 14:27:34: 33000000 INFO @ Sun, 03 Jun 2018 14:27:35: 32000000 INFO @ Sun, 03 Jun 2018 14:27:38: 32000000 INFO @ Sun, 03 Jun 2018 14:27:40: 34000000 INFO @ Sun, 03 Jun 2018 14:27:42: 33000000 INFO @ Sun, 03 Jun 2018 14:27:44: 33000000 INFO @ Sun, 03 Jun 2018 14:27:46: 35000000 INFO @ Sun, 03 Jun 2018 14:27:48: 34000000 INFO @ Sun, 03 Jun 2018 14:27:51: 34000000 INFO @ Sun, 03 Jun 2018 14:27:52: 36000000 INFO @ Sun, 03 Jun 2018 14:27:55: 35000000 INFO @ Sun, 03 Jun 2018 14:27:57: 35000000 INFO @ Sun, 03 Jun 2018 14:27:58: 37000000 INFO @ Sun, 03 Jun 2018 14:28:01: 36000000 INFO @ Sun, 03 Jun 2018 14:28:04: 36000000 INFO @ Sun, 03 Jun 2018 14:28:04: 38000000 INFO @ Sun, 03 Jun 2018 14:28:08: 37000000 INFO @ Sun, 03 Jun 2018 14:28:10: 39000000 INFO @ Sun, 03 Jun 2018 14:28:11: 37000000 INFO @ Sun, 03 Jun 2018 14:28:14: 38000000 INFO @ Sun, 03 Jun 2018 14:28:16: 40000000 INFO @ Sun, 03 Jun 2018 14:28:17: 38000000 INFO @ Sun, 03 Jun 2018 14:28:21: 39000000 INFO @ Sun, 03 Jun 2018 14:28:23: 41000000 INFO @ Sun, 03 Jun 2018 14:28:24: 39000000 INFO @ Sun, 03 Jun 2018 14:28:28: 40000000 INFO @ Sun, 03 Jun 2018 14:28:29: 42000000 INFO @ Sun, 03 Jun 2018 14:28:32: 40000000 INFO @ Sun, 03 Jun 2018 14:28:35: 43000000 INFO @ Sun, 03 Jun 2018 14:28:35: 41000000 INFO @ Sun, 03 Jun 2018 14:28:39: 41000000 INFO @ Sun, 03 Jun 2018 14:28:42: 44000000 INFO @ Sun, 03 Jun 2018 14:28:43: 42000000 INFO @ Sun, 03 Jun 2018 14:28:46: 42000000 INFO @ Sun, 03 Jun 2018 14:28:48: 45000000 INFO @ Sun, 03 Jun 2018 14:28:50: 43000000 INFO @ Sun, 03 Jun 2018 14:28:54: 43000000 INFO @ Sun, 03 Jun 2018 14:28:54: 46000000 INFO @ Sun, 03 Jun 2018 14:28:57: 44000000 INFO @ Sun, 03 Jun 2018 14:29:01: 47000000 INFO @ Sun, 03 Jun 2018 14:29:01: 44000000 INFO @ Sun, 03 Jun 2018 14:29:03: #1 tag size is determined as 50 bps INFO @ Sun, 03 Jun 2018 14:29:03: #1 tag size = 50 INFO @ Sun, 03 Jun 2018 14:29:03: #1 total tags in treatment: 47226742 INFO @ Sun, 03 Jun 2018 14:29:03: #1 user defined the maximum tags... INFO @ Sun, 03 Jun 2018 14:29:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 03 Jun 2018 14:29:04: #1 tags after filtering in treatment: 47226742 INFO @ Sun, 03 Jun 2018 14:29:04: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 03 Jun 2018 14:29:04: #1 finished! INFO @ Sun, 03 Jun 2018 14:29:04: #2 Build Peak Model... INFO @ Sun, 03 Jun 2018 14:29:04: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 03 Jun 2018 14:29:04: 45000000 INFO @ Sun, 03 Jun 2018 14:29:08: #2 number of paired peaks: 0 WARNING @ Sun, 03 Jun 2018 14:29:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 03 Jun 2018 14:29:08: Process for pairing-model is terminated! cat: SRX4047177.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4047177.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4047177.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4047177.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sun, 03 Jun 2018 14:29:08: 45000000 INFO @ Sun, 03 Jun 2018 14:29:11: 46000000 INFO @ Sun, 03 Jun 2018 14:29:16: 46000000 INFO @ Sun, 03 Jun 2018 14:29:19: 47000000 INFO @ Sun, 03 Jun 2018 14:29:21: #1 tag size is determined as 50 bps INFO @ Sun, 03 Jun 2018 14:29:21: #1 tag size = 50 INFO @ Sun, 03 Jun 2018 14:29:21: #1 total tags in treatment: 47226742 INFO @ Sun, 03 Jun 2018 14:29:21: #1 user defined the maximum tags... INFO @ Sun, 03 Jun 2018 14:29:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 03 Jun 2018 14:29:22: #1 tags after filtering in treatment: 47226742 INFO @ Sun, 03 Jun 2018 14:29:22: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 03 Jun 2018 14:29:22: #1 finished! INFO @ Sun, 03 Jun 2018 14:29:22: #2 Build Peak Model... INFO @ Sun, 03 Jun 2018 14:29:22: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 03 Jun 2018 14:29:23: 47000000 INFO @ Sun, 03 Jun 2018 14:29:25: #2 number of paired peaks: 0 WARNING @ Sun, 03 Jun 2018 14:29:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 03 Jun 2018 14:29:25: Process for pairing-model is terminated! cat: SRX4047177.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4047177.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4047177.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4047177.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sun, 03 Jun 2018 14:29:25: #1 tag size is determined as 50 bps INFO @ Sun, 03 Jun 2018 14:29:25: #1 tag size = 50 INFO @ Sun, 03 Jun 2018 14:29:25: #1 total tags in treatment: 47226742 INFO @ Sun, 03 Jun 2018 14:29:25: #1 user defined the maximum tags... INFO @ Sun, 03 Jun 2018 14:29:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 03 Jun 2018 14:29:26: #1 tags after filtering in treatment: 47226742 INFO @ Sun, 03 Jun 2018 14:29:26: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 03 Jun 2018 14:29:26: #1 finished! INFO @ Sun, 03 Jun 2018 14:29:26: #2 Build Peak Model... INFO @ Sun, 03 Jun 2018 14:29:26: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 03 Jun 2018 14:29:29: #2 number of paired peaks: 0 WARNING @ Sun, 03 Jun 2018 14:29:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 03 Jun 2018 14:29:29: Process for pairing-model is terminated! cat: SRX4047177.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4047177.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4047177.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4047177.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。