Job ID = 1295702 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 33,788,440 reads read : 33,788,440 reads written : 33,788,440 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:32 33788440 reads; of these: 33788440 (100.00%) were unpaired; of these: 1171461 (3.47%) aligned 0 times 24233409 (71.72%) aligned exactly 1 time 8383570 (24.81%) aligned >1 times 96.53% overall alignment rate Time searching: 00:12:32 Overall time: 00:12:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 4936263 / 32616979 = 0.1513 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 15:33:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX403441/SRX403441.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX403441/SRX403441.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX403441/SRX403441.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX403441/SRX403441.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 15:33:25: #1 read tag files... INFO @ Mon, 03 Jun 2019 15:33:25: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 15:33:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX403441/SRX403441.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX403441/SRX403441.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX403441/SRX403441.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX403441/SRX403441.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 15:33:25: #1 read tag files... INFO @ Mon, 03 Jun 2019 15:33:25: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 15:33:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX403441/SRX403441.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX403441/SRX403441.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX403441/SRX403441.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX403441/SRX403441.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 15:33:26: #1 read tag files... INFO @ Mon, 03 Jun 2019 15:33:26: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 15:33:34: 1000000 INFO @ Mon, 03 Jun 2019 15:33:35: 1000000 INFO @ Mon, 03 Jun 2019 15:33:35: 1000000 INFO @ Mon, 03 Jun 2019 15:33:42: 2000000 INFO @ Mon, 03 Jun 2019 15:33:45: 2000000 INFO @ Mon, 03 Jun 2019 15:33:45: 2000000 INFO @ Mon, 03 Jun 2019 15:33:50: 3000000 INFO @ Mon, 03 Jun 2019 15:33:55: 3000000 INFO @ Mon, 03 Jun 2019 15:33:55: 3000000 INFO @ Mon, 03 Jun 2019 15:33:58: 4000000 INFO @ Mon, 03 Jun 2019 15:34:05: 4000000 INFO @ Mon, 03 Jun 2019 15:34:05: 4000000 INFO @ Mon, 03 Jun 2019 15:34:06: 5000000 INFO @ Mon, 03 Jun 2019 15:34:14: 6000000 INFO @ Mon, 03 Jun 2019 15:34:15: 5000000 INFO @ Mon, 03 Jun 2019 15:34:15: 5000000 INFO @ Mon, 03 Jun 2019 15:34:21: 7000000 INFO @ Mon, 03 Jun 2019 15:34:24: 6000000 INFO @ Mon, 03 Jun 2019 15:34:24: 6000000 INFO @ Mon, 03 Jun 2019 15:34:29: 8000000 INFO @ Mon, 03 Jun 2019 15:34:34: 7000000 INFO @ Mon, 03 Jun 2019 15:34:34: 7000000 INFO @ Mon, 03 Jun 2019 15:34:37: 9000000 INFO @ Mon, 03 Jun 2019 15:34:43: 8000000 INFO @ Mon, 03 Jun 2019 15:34:43: 8000000 INFO @ Mon, 03 Jun 2019 15:34:45: 10000000 INFO @ Mon, 03 Jun 2019 15:34:52: 11000000 INFO @ Mon, 03 Jun 2019 15:34:52: 9000000 INFO @ Mon, 03 Jun 2019 15:34:52: 9000000 INFO @ Mon, 03 Jun 2019 15:35:00: 12000000 INFO @ Mon, 03 Jun 2019 15:35:02: 10000000 INFO @ Mon, 03 Jun 2019 15:35:02: 10000000 INFO @ Mon, 03 Jun 2019 15:35:07: 13000000 INFO @ Mon, 03 Jun 2019 15:35:11: 11000000 INFO @ Mon, 03 Jun 2019 15:35:11: 11000000 INFO @ Mon, 03 Jun 2019 15:35:15: 14000000 INFO @ Mon, 03 Jun 2019 15:35:20: 12000000 INFO @ Mon, 03 Jun 2019 15:35:20: 12000000 INFO @ Mon, 03 Jun 2019 15:35:23: 15000000 INFO @ Mon, 03 Jun 2019 15:35:29: 13000000 INFO @ Mon, 03 Jun 2019 15:35:29: 13000000 INFO @ Mon, 03 Jun 2019 15:35:31: 16000000 INFO @ Mon, 03 Jun 2019 15:35:38: 17000000 INFO @ Mon, 03 Jun 2019 15:35:39: 14000000 INFO @ Mon, 03 Jun 2019 15:35:39: 14000000 INFO @ Mon, 03 Jun 2019 15:35:46: 18000000 INFO @ Mon, 03 Jun 2019 15:35:48: 15000000 INFO @ Mon, 03 Jun 2019 15:35:48: 15000000 INFO @ Mon, 03 Jun 2019 15:35:54: 19000000 INFO @ Mon, 03 Jun 2019 15:35:57: 16000000 INFO @ Mon, 03 Jun 2019 15:35:57: 16000000 INFO @ Mon, 03 Jun 2019 15:36:01: 20000000 INFO @ Mon, 03 Jun 2019 15:36:06: 17000000 INFO @ Mon, 03 Jun 2019 15:36:06: 17000000 INFO @ Mon, 03 Jun 2019 15:36:09: 21000000 INFO @ Mon, 03 Jun 2019 15:36:16: 18000000 INFO @ Mon, 03 Jun 2019 15:36:16: 18000000 INFO @ Mon, 03 Jun 2019 15:36:17: 22000000 INFO @ Mon, 03 Jun 2019 15:36:24: 23000000 INFO @ Mon, 03 Jun 2019 15:36:25: 19000000 INFO @ Mon, 03 Jun 2019 15:36:25: 19000000 INFO @ Mon, 03 Jun 2019 15:36:32: 24000000 INFO @ Mon, 03 Jun 2019 15:36:34: 20000000 INFO @ Mon, 03 Jun 2019 15:36:35: 20000000 INFO @ Mon, 03 Jun 2019 15:36:40: 25000000 INFO @ Mon, 03 Jun 2019 15:36:43: 21000000 INFO @ Mon, 03 Jun 2019 15:36:45: 21000000 INFO @ Mon, 03 Jun 2019 15:36:48: 26000000 INFO @ Mon, 03 Jun 2019 15:36:52: 22000000 INFO @ Mon, 03 Jun 2019 15:36:54: 22000000 INFO @ Mon, 03 Jun 2019 15:36:55: 27000000 INFO @ Mon, 03 Jun 2019 15:37:01: #1 tag size is determined as 36 bps INFO @ Mon, 03 Jun 2019 15:37:01: #1 tag size = 36 INFO @ Mon, 03 Jun 2019 15:37:01: #1 total tags in treatment: 27680716 INFO @ Mon, 03 Jun 2019 15:37:01: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 15:37:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 15:37:01: #1 tags after filtering in treatment: 27680716 INFO @ Mon, 03 Jun 2019 15:37:01: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 15:37:01: #1 finished! INFO @ Mon, 03 Jun 2019 15:37:01: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 15:37:01: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 15:37:01: 23000000 INFO @ Mon, 03 Jun 2019 15:37:04: 23000000 INFO @ Mon, 03 Jun 2019 15:37:04: #2 number of paired peaks: 25 WARNING @ Mon, 03 Jun 2019 15:37:04: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 15:37:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX403441/SRX403441.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX403441/SRX403441.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX403441/SRX403441.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX403441/SRX403441.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 15:37:10: 24000000 INFO @ Mon, 03 Jun 2019 15:37:13: 24000000 INFO @ Mon, 03 Jun 2019 15:37:19: 25000000 INFO @ Mon, 03 Jun 2019 15:37:22: 25000000 INFO @ Mon, 03 Jun 2019 15:37:28: 26000000 INFO @ Mon, 03 Jun 2019 15:37:32: 26000000 INFO @ Mon, 03 Jun 2019 15:37:37: 27000000 INFO @ Mon, 03 Jun 2019 15:37:41: 27000000 INFO @ Mon, 03 Jun 2019 15:37:43: #1 tag size is determined as 36 bps INFO @ Mon, 03 Jun 2019 15:37:43: #1 tag size = 36 INFO @ Mon, 03 Jun 2019 15:37:43: #1 total tags in treatment: 27680716 INFO @ Mon, 03 Jun 2019 15:37:43: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 15:37:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 15:37:44: #1 tags after filtering in treatment: 27680716 INFO @ Mon, 03 Jun 2019 15:37:44: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 15:37:44: #1 finished! INFO @ Mon, 03 Jun 2019 15:37:44: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 15:37:44: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 15:37:46: #2 number of paired peaks: 25 WARNING @ Mon, 03 Jun 2019 15:37:46: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 15:37:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX403441/SRX403441.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX403441/SRX403441.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX403441/SRX403441.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX403441/SRX403441.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 15:37:47: #1 tag size is determined as 36 bps INFO @ Mon, 03 Jun 2019 15:37:47: #1 tag size = 36 INFO @ Mon, 03 Jun 2019 15:37:47: #1 total tags in treatment: 27680716 INFO @ Mon, 03 Jun 2019 15:37:47: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 15:37:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 15:37:47: #1 tags after filtering in treatment: 27680716 INFO @ Mon, 03 Jun 2019 15:37:47: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 15:37:47: #1 finished! INFO @ Mon, 03 Jun 2019 15:37:47: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 15:37:47: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 15:37:50: #2 number of paired peaks: 25 WARNING @ Mon, 03 Jun 2019 15:37:50: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 15:37:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX403441/SRX403441.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX403441/SRX403441.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX403441/SRX403441.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX403441/SRX403441.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。