Job ID = 1295698 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 34,612,851 reads read : 34,612,851 reads written : 34,612,851 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:19 34612851 reads; of these: 34612851 (100.00%) were unpaired; of these: 1345641 (3.89%) aligned 0 times 25105622 (72.53%) aligned exactly 1 time 8161588 (23.58%) aligned >1 times 96.11% overall alignment rate Time searching: 00:11:19 Overall time: 00:11:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5544351 / 33267210 = 0.1667 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 15:31:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX403440/SRX403440.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX403440/SRX403440.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX403440/SRX403440.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX403440/SRX403440.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 15:31:37: #1 read tag files... INFO @ Mon, 03 Jun 2019 15:31:37: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 15:31:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX403440/SRX403440.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX403440/SRX403440.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX403440/SRX403440.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX403440/SRX403440.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 15:31:37: #1 read tag files... INFO @ Mon, 03 Jun 2019 15:31:37: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 15:31:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX403440/SRX403440.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX403440/SRX403440.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX403440/SRX403440.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX403440/SRX403440.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 15:31:37: #1 read tag files... INFO @ Mon, 03 Jun 2019 15:31:37: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 15:31:45: 1000000 INFO @ Mon, 03 Jun 2019 15:31:46: 1000000 INFO @ Mon, 03 Jun 2019 15:31:46: 1000000 INFO @ Mon, 03 Jun 2019 15:31:51: 2000000 INFO @ Mon, 03 Jun 2019 15:31:53: 2000000 INFO @ Mon, 03 Jun 2019 15:31:54: 2000000 INFO @ Mon, 03 Jun 2019 15:31:57: 3000000 INFO @ Mon, 03 Jun 2019 15:32:01: 3000000 INFO @ Mon, 03 Jun 2019 15:32:02: 3000000 INFO @ Mon, 03 Jun 2019 15:32:04: 4000000 INFO @ Mon, 03 Jun 2019 15:32:08: 4000000 INFO @ Mon, 03 Jun 2019 15:32:09: 4000000 INFO @ Mon, 03 Jun 2019 15:32:10: 5000000 INFO @ Mon, 03 Jun 2019 15:32:16: 5000000 INFO @ Mon, 03 Jun 2019 15:32:16: 6000000 INFO @ Mon, 03 Jun 2019 15:32:16: 5000000 INFO @ Mon, 03 Jun 2019 15:32:22: 7000000 INFO @ Mon, 03 Jun 2019 15:32:23: 6000000 INFO @ Mon, 03 Jun 2019 15:32:24: 6000000 INFO @ Mon, 03 Jun 2019 15:32:29: 8000000 INFO @ Mon, 03 Jun 2019 15:32:31: 7000000 INFO @ Mon, 03 Jun 2019 15:32:31: 7000000 INFO @ Mon, 03 Jun 2019 15:32:35: 9000000 INFO @ Mon, 03 Jun 2019 15:32:38: 8000000 INFO @ Mon, 03 Jun 2019 15:32:38: 8000000 INFO @ Mon, 03 Jun 2019 15:32:41: 10000000 INFO @ Mon, 03 Jun 2019 15:32:45: 9000000 INFO @ Mon, 03 Jun 2019 15:32:45: 9000000 INFO @ Mon, 03 Jun 2019 15:32:47: 11000000 INFO @ Mon, 03 Jun 2019 15:32:53: 10000000 INFO @ Mon, 03 Jun 2019 15:32:53: 10000000 INFO @ Mon, 03 Jun 2019 15:32:54: 12000000 INFO @ Mon, 03 Jun 2019 15:33:00: 13000000 INFO @ Mon, 03 Jun 2019 15:33:00: 11000000 INFO @ Mon, 03 Jun 2019 15:33:01: 11000000 INFO @ Mon, 03 Jun 2019 15:33:06: 14000000 INFO @ Mon, 03 Jun 2019 15:33:08: 12000000 INFO @ Mon, 03 Jun 2019 15:33:08: 12000000 INFO @ Mon, 03 Jun 2019 15:33:13: 15000000 INFO @ Mon, 03 Jun 2019 15:33:15: 13000000 INFO @ Mon, 03 Jun 2019 15:33:16: 13000000 INFO @ Mon, 03 Jun 2019 15:33:19: 16000000 INFO @ Mon, 03 Jun 2019 15:33:23: 14000000 INFO @ Mon, 03 Jun 2019 15:33:23: 14000000 INFO @ Mon, 03 Jun 2019 15:33:25: 17000000 INFO @ Mon, 03 Jun 2019 15:33:31: 15000000 INFO @ Mon, 03 Jun 2019 15:33:31: 15000000 INFO @ Mon, 03 Jun 2019 15:33:32: 18000000 INFO @ Mon, 03 Jun 2019 15:33:38: 19000000 INFO @ Mon, 03 Jun 2019 15:33:39: 16000000 INFO @ Mon, 03 Jun 2019 15:33:39: 16000000 INFO @ Mon, 03 Jun 2019 15:33:44: 20000000 INFO @ Mon, 03 Jun 2019 15:33:46: 17000000 INFO @ Mon, 03 Jun 2019 15:33:46: 17000000 INFO @ Mon, 03 Jun 2019 15:33:50: 21000000 INFO @ Mon, 03 Jun 2019 15:33:54: 18000000 INFO @ Mon, 03 Jun 2019 15:33:54: 18000000 INFO @ Mon, 03 Jun 2019 15:33:57: 22000000 INFO @ Mon, 03 Jun 2019 15:34:01: 19000000 INFO @ Mon, 03 Jun 2019 15:34:01: 19000000 INFO @ Mon, 03 Jun 2019 15:34:03: 23000000 INFO @ Mon, 03 Jun 2019 15:34:08: 20000000 INFO @ Mon, 03 Jun 2019 15:34:09: 20000000 INFO @ Mon, 03 Jun 2019 15:34:09: 24000000 INFO @ Mon, 03 Jun 2019 15:34:16: 25000000 INFO @ Mon, 03 Jun 2019 15:34:16: 21000000 INFO @ Mon, 03 Jun 2019 15:34:16: 21000000 INFO @ Mon, 03 Jun 2019 15:34:22: 26000000 INFO @ Mon, 03 Jun 2019 15:34:23: 22000000 INFO @ Mon, 03 Jun 2019 15:34:25: 22000000 INFO @ Mon, 03 Jun 2019 15:34:28: 27000000 INFO @ Mon, 03 Jun 2019 15:34:30: 23000000 INFO @ Mon, 03 Jun 2019 15:34:32: 23000000 INFO @ Mon, 03 Jun 2019 15:34:33: #1 tag size is determined as 36 bps INFO @ Mon, 03 Jun 2019 15:34:33: #1 tag size = 36 INFO @ Mon, 03 Jun 2019 15:34:33: #1 total tags in treatment: 27722859 INFO @ Mon, 03 Jun 2019 15:34:33: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 15:34:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 15:34:34: #1 tags after filtering in treatment: 27722859 INFO @ Mon, 03 Jun 2019 15:34:34: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 15:34:34: #1 finished! INFO @ Mon, 03 Jun 2019 15:34:34: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 15:34:34: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 15:34:36: #2 number of paired peaks: 23 WARNING @ Mon, 03 Jun 2019 15:34:36: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 15:34:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX403440/SRX403440.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX403440/SRX403440.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX403440/SRX403440.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX403440/SRX403440.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 15:34:38: 24000000 INFO @ Mon, 03 Jun 2019 15:34:40: 24000000 INFO @ Mon, 03 Jun 2019 15:34:46: 25000000 INFO @ Mon, 03 Jun 2019 15:34:47: 25000000 INFO @ Mon, 03 Jun 2019 15:34:53: 26000000 INFO @ Mon, 03 Jun 2019 15:34:55: 26000000 INFO @ Mon, 03 Jun 2019 15:35:00: 27000000 INFO @ Mon, 03 Jun 2019 15:35:02: 27000000 INFO @ Mon, 03 Jun 2019 15:35:05: #1 tag size is determined as 36 bps INFO @ Mon, 03 Jun 2019 15:35:05: #1 tag size = 36 INFO @ Mon, 03 Jun 2019 15:35:05: #1 total tags in treatment: 27722859 INFO @ Mon, 03 Jun 2019 15:35:05: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 15:35:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 15:35:06: #1 tags after filtering in treatment: 27722859 INFO @ Mon, 03 Jun 2019 15:35:06: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 15:35:06: #1 finished! INFO @ Mon, 03 Jun 2019 15:35:06: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 15:35:06: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 15:35:08: #1 tag size is determined as 36 bps INFO @ Mon, 03 Jun 2019 15:35:08: #1 tag size = 36 INFO @ Mon, 03 Jun 2019 15:35:08: #1 total tags in treatment: 27722859 INFO @ Mon, 03 Jun 2019 15:35:08: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 15:35:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 15:35:08: #2 number of paired peaks: 23 WARNING @ Mon, 03 Jun 2019 15:35:08: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 15:35:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX403440/SRX403440.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX403440/SRX403440.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX403440/SRX403440.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX403440/SRX403440.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 15:35:08: #1 tags after filtering in treatment: 27722859 INFO @ Mon, 03 Jun 2019 15:35:08: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 15:35:08: #1 finished! INFO @ Mon, 03 Jun 2019 15:35:08: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 15:35:08: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 15:35:10: #2 number of paired peaks: 23 WARNING @ Mon, 03 Jun 2019 15:35:10: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 15:35:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX403440/SRX403440.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX403440/SRX403440.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX403440/SRX403440.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX403440/SRX403440.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。