Job ID = 2590499 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 14,878,199 reads read : 29,756,398 reads written : 14,878,199 reads 0-length : 14,878,199 spots read : 15,054,011 reads read : 30,108,022 reads written : 15,054,011 reads 0-length : 15,054,011 spots read : 15,134,321 reads read : 30,268,642 reads written : 15,134,321 reads 0-length : 15,134,321 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:18:13 45066531 reads; of these: 45066531 (100.00%) were unpaired; of these: 1300424 (2.89%) aligned 0 times 31547527 (70.00%) aligned exactly 1 time 12218580 (27.11%) aligned >1 times 97.11% overall alignment rate Time searching: 00:18:13 Overall time: 00:18:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 5425302 / 43766107 = 0.1240 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 22:45:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981726/SRX3981726.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981726/SRX3981726.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981726/SRX3981726.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981726/SRX3981726.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:45:38: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:45:38: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:45:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981726/SRX3981726.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981726/SRX3981726.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981726/SRX3981726.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981726/SRX3981726.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:45:39: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:45:39: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:45:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981726/SRX3981726.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981726/SRX3981726.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981726/SRX3981726.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981726/SRX3981726.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:45:40: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:45:40: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:45:45: 1000000 INFO @ Mon, 12 Aug 2019 22:45:46: 1000000 INFO @ Mon, 12 Aug 2019 22:45:47: 1000000 INFO @ Mon, 12 Aug 2019 22:45:52: 2000000 INFO @ Mon, 12 Aug 2019 22:45:53: 2000000 INFO @ Mon, 12 Aug 2019 22:45:53: 2000000 INFO @ Mon, 12 Aug 2019 22:45:58: 3000000 INFO @ Mon, 12 Aug 2019 22:45:59: 3000000 INFO @ Mon, 12 Aug 2019 22:45:59: 3000000 INFO @ Mon, 12 Aug 2019 22:46:04: 4000000 INFO @ Mon, 12 Aug 2019 22:46:05: 4000000 INFO @ Mon, 12 Aug 2019 22:46:06: 4000000 INFO @ Mon, 12 Aug 2019 22:46:11: 5000000 INFO @ Mon, 12 Aug 2019 22:46:11: 5000000 INFO @ Mon, 12 Aug 2019 22:46:12: 5000000 INFO @ Mon, 12 Aug 2019 22:46:17: 6000000 INFO @ Mon, 12 Aug 2019 22:46:17: 6000000 INFO @ Mon, 12 Aug 2019 22:46:19: 6000000 INFO @ Mon, 12 Aug 2019 22:46:23: 7000000 INFO @ Mon, 12 Aug 2019 22:46:24: 7000000 INFO @ Mon, 12 Aug 2019 22:46:25: 7000000 INFO @ Mon, 12 Aug 2019 22:46:30: 8000000 INFO @ Mon, 12 Aug 2019 22:46:30: 8000000 INFO @ Mon, 12 Aug 2019 22:46:32: 8000000 INFO @ Mon, 12 Aug 2019 22:46:36: 9000000 INFO @ Mon, 12 Aug 2019 22:46:37: 9000000 INFO @ Mon, 12 Aug 2019 22:46:38: 9000000 INFO @ Mon, 12 Aug 2019 22:46:42: 10000000 INFO @ Mon, 12 Aug 2019 22:46:43: 10000000 INFO @ Mon, 12 Aug 2019 22:46:44: 10000000 INFO @ Mon, 12 Aug 2019 22:46:48: 11000000 INFO @ Mon, 12 Aug 2019 22:46:50: 11000000 INFO @ Mon, 12 Aug 2019 22:46:51: 11000000 INFO @ Mon, 12 Aug 2019 22:46:54: 12000000 INFO @ Mon, 12 Aug 2019 22:46:56: 12000000 INFO @ Mon, 12 Aug 2019 22:46:57: 12000000 INFO @ Mon, 12 Aug 2019 22:47:00: 13000000 INFO @ Mon, 12 Aug 2019 22:47:02: 13000000 INFO @ Mon, 12 Aug 2019 22:47:04: 13000000 INFO @ Mon, 12 Aug 2019 22:47:06: 14000000 INFO @ Mon, 12 Aug 2019 22:47:10: 14000000 INFO @ Mon, 12 Aug 2019 22:47:10: 14000000 INFO @ Mon, 12 Aug 2019 22:47:12: 15000000 INFO @ Mon, 12 Aug 2019 22:47:17: 15000000 INFO @ Mon, 12 Aug 2019 22:47:17: 15000000 INFO @ Mon, 12 Aug 2019 22:47:18: 16000000 INFO @ Mon, 12 Aug 2019 22:47:24: 16000000 INFO @ Mon, 12 Aug 2019 22:47:24: 16000000 INFO @ Mon, 12 Aug 2019 22:47:24: 17000000 INFO @ Mon, 12 Aug 2019 22:47:30: 17000000 INFO @ Mon, 12 Aug 2019 22:47:30: 18000000 INFO @ Mon, 12 Aug 2019 22:47:30: 17000000 INFO @ Mon, 12 Aug 2019 22:47:37: 19000000 INFO @ Mon, 12 Aug 2019 22:47:37: 18000000 INFO @ Mon, 12 Aug 2019 22:47:37: 18000000 INFO @ Mon, 12 Aug 2019 22:47:43: 20000000 INFO @ Mon, 12 Aug 2019 22:47:43: 19000000 INFO @ Mon, 12 Aug 2019 22:47:43: 19000000 INFO @ Mon, 12 Aug 2019 22:47:49: 21000000 INFO @ Mon, 12 Aug 2019 22:47:49: 20000000 INFO @ Mon, 12 Aug 2019 22:47:50: 20000000 INFO @ Mon, 12 Aug 2019 22:47:55: 22000000 INFO @ Mon, 12 Aug 2019 22:47:56: 21000000 INFO @ Mon, 12 Aug 2019 22:47:56: 21000000 INFO @ Mon, 12 Aug 2019 22:48:01: 23000000 INFO @ Mon, 12 Aug 2019 22:48:02: 22000000 INFO @ Mon, 12 Aug 2019 22:48:03: 22000000 INFO @ Mon, 12 Aug 2019 22:48:07: 24000000 INFO @ Mon, 12 Aug 2019 22:48:09: 23000000 INFO @ Mon, 12 Aug 2019 22:48:09: 23000000 INFO @ Mon, 12 Aug 2019 22:48:13: 25000000 INFO @ Mon, 12 Aug 2019 22:48:15: 24000000 INFO @ Mon, 12 Aug 2019 22:48:16: 24000000 INFO @ Mon, 12 Aug 2019 22:48:19: 26000000 INFO @ Mon, 12 Aug 2019 22:48:22: 25000000 INFO @ Mon, 12 Aug 2019 22:48:23: 25000000 INFO @ Mon, 12 Aug 2019 22:48:25: 27000000 INFO @ Mon, 12 Aug 2019 22:48:28: 26000000 INFO @ Mon, 12 Aug 2019 22:48:30: 26000000 INFO @ Mon, 12 Aug 2019 22:48:31: 28000000 INFO @ Mon, 12 Aug 2019 22:48:35: 27000000 INFO @ Mon, 12 Aug 2019 22:48:37: 27000000 INFO @ Mon, 12 Aug 2019 22:48:37: 29000000 INFO @ Mon, 12 Aug 2019 22:48:41: 28000000 INFO @ Mon, 12 Aug 2019 22:48:43: 28000000 INFO @ Mon, 12 Aug 2019 22:48:44: 30000000 INFO @ Mon, 12 Aug 2019 22:48:48: 29000000 INFO @ Mon, 12 Aug 2019 22:48:49: 29000000 INFO @ Mon, 12 Aug 2019 22:48:50: 31000000 INFO @ Mon, 12 Aug 2019 22:48:54: 30000000 INFO @ Mon, 12 Aug 2019 22:48:56: 30000000 INFO @ Mon, 12 Aug 2019 22:48:56: 32000000 INFO @ Mon, 12 Aug 2019 22:49:01: 31000000 INFO @ Mon, 12 Aug 2019 22:49:02: 31000000 INFO @ Mon, 12 Aug 2019 22:49:02: 33000000 INFO @ Mon, 12 Aug 2019 22:49:07: 32000000 INFO @ Mon, 12 Aug 2019 22:49:09: 34000000 INFO @ Mon, 12 Aug 2019 22:49:09: 32000000 INFO @ Mon, 12 Aug 2019 22:49:14: 33000000 INFO @ Mon, 12 Aug 2019 22:49:15: 35000000 INFO @ Mon, 12 Aug 2019 22:49:15: 33000000 INFO @ Mon, 12 Aug 2019 22:49:20: 34000000 INFO @ Mon, 12 Aug 2019 22:49:21: 36000000 INFO @ Mon, 12 Aug 2019 22:49:22: 34000000 INFO @ Mon, 12 Aug 2019 22:49:27: 35000000 INFO @ Mon, 12 Aug 2019 22:49:27: 37000000 INFO @ Mon, 12 Aug 2019 22:49:29: 35000000 INFO @ Mon, 12 Aug 2019 22:49:33: 38000000 INFO @ Mon, 12 Aug 2019 22:49:33: 36000000 INFO @ Mon, 12 Aug 2019 22:49:36: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:49:36: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:49:36: #1 total tags in treatment: 38340805 INFO @ Mon, 12 Aug 2019 22:49:36: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:49:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:49:36: 36000000 INFO @ Mon, 12 Aug 2019 22:49:36: #1 tags after filtering in treatment: 38340805 INFO @ Mon, 12 Aug 2019 22:49:36: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:49:36: #1 finished! INFO @ Mon, 12 Aug 2019 22:49:36: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:49:36: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:49:40: #2 number of paired peaks: 4 WARNING @ Mon, 12 Aug 2019 22:49:40: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:49:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981726/SRX3981726.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981726/SRX3981726.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981726/SRX3981726.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981726/SRX3981726.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 22:49:40: 37000000 INFO @ Mon, 12 Aug 2019 22:49:43: 37000000 INFO @ Mon, 12 Aug 2019 22:49:47: 38000000 INFO @ Mon, 12 Aug 2019 22:49:49: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:49:49: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:49:49: #1 total tags in treatment: 38340805 INFO @ Mon, 12 Aug 2019 22:49:49: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:49:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:49:50: 38000000 INFO @ Mon, 12 Aug 2019 22:49:50: #1 tags after filtering in treatment: 38340805 INFO @ Mon, 12 Aug 2019 22:49:50: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:49:50: #1 finished! INFO @ Mon, 12 Aug 2019 22:49:50: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:49:50: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:49:53: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:49:53: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:49:53: #1 total tags in treatment: 38340805 INFO @ Mon, 12 Aug 2019 22:49:53: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:49:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:49:53: #2 number of paired peaks: 4 WARNING @ Mon, 12 Aug 2019 22:49:53: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:49:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981726/SRX3981726.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) INFO @ Mon, 12 Aug 2019 22:49:53: #1 tags after filtering in treatment: 38340805 INFO @ Mon, 12 Aug 2019 22:49:53: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:49:53: #1 finished! INFO @ Mon, 12 Aug 2019 22:49:53: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:49:53: #2 looking for paired plus/minus strand peaks... rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981726/SRX3981726.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981726/SRX3981726.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981726/SRX3981726.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 22:49:56: #2 number of paired peaks: 4 WARNING @ Mon, 12 Aug 2019 22:49:56: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:49:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981726/SRX3981726.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981726/SRX3981726.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981726/SRX3981726.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981726/SRX3981726.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。