Job ID = 2590498 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 12,567,631 reads read : 25,135,262 reads written : 12,567,631 reads 0-length : 12,567,631 spots read : 12,732,519 reads read : 25,465,038 reads written : 12,732,519 reads 0-length : 12,732,519 spots read : 12,757,691 reads read : 25,515,382 reads written : 12,757,691 reads 0-length : 12,757,691 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:24 38057841 reads; of these: 38057841 (100.00%) were unpaired; of these: 1054812 (2.77%) aligned 0 times 26582525 (69.85%) aligned exactly 1 time 10420504 (27.38%) aligned >1 times 97.23% overall alignment rate Time searching: 00:14:25 Overall time: 00:14:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4268333 / 37003029 = 0.1154 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 22:37:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981725/SRX3981725.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981725/SRX3981725.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981725/SRX3981725.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981725/SRX3981725.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:37:19: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:37:19: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:37:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981725/SRX3981725.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981725/SRX3981725.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981725/SRX3981725.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981725/SRX3981725.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:37:20: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:37:20: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:37:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981725/SRX3981725.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981725/SRX3981725.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981725/SRX3981725.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981725/SRX3981725.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:37:21: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:37:21: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:37:27: 1000000 INFO @ Mon, 12 Aug 2019 22:37:27: 1000000 INFO @ Mon, 12 Aug 2019 22:37:28: 1000000 INFO @ Mon, 12 Aug 2019 22:37:33: 2000000 INFO @ Mon, 12 Aug 2019 22:37:34: 2000000 INFO @ Mon, 12 Aug 2019 22:37:35: 2000000 INFO @ Mon, 12 Aug 2019 22:37:40: 3000000 INFO @ Mon, 12 Aug 2019 22:37:41: 3000000 INFO @ Mon, 12 Aug 2019 22:37:41: 3000000 INFO @ Mon, 12 Aug 2019 22:37:47: 4000000 INFO @ Mon, 12 Aug 2019 22:37:48: 4000000 INFO @ Mon, 12 Aug 2019 22:37:48: 4000000 INFO @ Mon, 12 Aug 2019 22:37:54: 5000000 INFO @ Mon, 12 Aug 2019 22:37:55: 5000000 INFO @ Mon, 12 Aug 2019 22:37:55: 5000000 INFO @ Mon, 12 Aug 2019 22:38:00: 6000000 INFO @ Mon, 12 Aug 2019 22:38:02: 6000000 INFO @ Mon, 12 Aug 2019 22:38:02: 6000000 INFO @ Mon, 12 Aug 2019 22:38:07: 7000000 INFO @ Mon, 12 Aug 2019 22:38:09: 7000000 INFO @ Mon, 12 Aug 2019 22:38:09: 7000000 INFO @ Mon, 12 Aug 2019 22:38:14: 8000000 INFO @ Mon, 12 Aug 2019 22:38:15: 8000000 INFO @ Mon, 12 Aug 2019 22:38:15: 8000000 INFO @ Mon, 12 Aug 2019 22:38:20: 9000000 INFO @ Mon, 12 Aug 2019 22:38:22: 9000000 INFO @ Mon, 12 Aug 2019 22:38:22: 9000000 INFO @ Mon, 12 Aug 2019 22:38:27: 10000000 INFO @ Mon, 12 Aug 2019 22:38:29: 10000000 INFO @ Mon, 12 Aug 2019 22:38:29: 10000000 INFO @ Mon, 12 Aug 2019 22:38:34: 11000000 INFO @ Mon, 12 Aug 2019 22:38:36: 11000000 INFO @ Mon, 12 Aug 2019 22:38:36: 11000000 INFO @ Mon, 12 Aug 2019 22:38:40: 12000000 INFO @ Mon, 12 Aug 2019 22:38:43: 12000000 INFO @ Mon, 12 Aug 2019 22:38:43: 12000000 INFO @ Mon, 12 Aug 2019 22:38:48: 13000000 INFO @ Mon, 12 Aug 2019 22:38:50: 13000000 INFO @ Mon, 12 Aug 2019 22:38:50: 13000000 INFO @ Mon, 12 Aug 2019 22:38:54: 14000000 INFO @ Mon, 12 Aug 2019 22:38:57: 14000000 INFO @ Mon, 12 Aug 2019 22:38:57: 14000000 INFO @ Mon, 12 Aug 2019 22:39:01: 15000000 INFO @ Mon, 12 Aug 2019 22:39:04: 15000000 INFO @ Mon, 12 Aug 2019 22:39:04: 15000000 INFO @ Mon, 12 Aug 2019 22:39:08: 16000000 INFO @ Mon, 12 Aug 2019 22:39:10: 16000000 INFO @ Mon, 12 Aug 2019 22:39:10: 16000000 INFO @ Mon, 12 Aug 2019 22:39:14: 17000000 INFO @ Mon, 12 Aug 2019 22:39:17: 17000000 INFO @ Mon, 12 Aug 2019 22:39:17: 17000000 INFO @ Mon, 12 Aug 2019 22:39:21: 18000000 INFO @ Mon, 12 Aug 2019 22:39:24: 18000000 INFO @ Mon, 12 Aug 2019 22:39:24: 18000000 INFO @ Mon, 12 Aug 2019 22:39:27: 19000000 INFO @ Mon, 12 Aug 2019 22:39:30: 19000000 INFO @ Mon, 12 Aug 2019 22:39:30: 19000000 INFO @ Mon, 12 Aug 2019 22:39:34: 20000000 INFO @ Mon, 12 Aug 2019 22:39:37: 20000000 INFO @ Mon, 12 Aug 2019 22:39:37: 20000000 INFO @ Mon, 12 Aug 2019 22:39:41: 21000000 INFO @ Mon, 12 Aug 2019 22:39:44: 21000000 INFO @ Mon, 12 Aug 2019 22:39:44: 21000000 INFO @ Mon, 12 Aug 2019 22:39:48: 22000000 INFO @ Mon, 12 Aug 2019 22:39:51: 22000000 INFO @ Mon, 12 Aug 2019 22:39:51: 22000000 INFO @ Mon, 12 Aug 2019 22:39:54: 23000000 INFO @ Mon, 12 Aug 2019 22:39:58: 23000000 INFO @ Mon, 12 Aug 2019 22:39:58: 23000000 INFO @ Mon, 12 Aug 2019 22:40:01: 24000000 INFO @ Mon, 12 Aug 2019 22:40:05: 24000000 INFO @ Mon, 12 Aug 2019 22:40:05: 24000000 INFO @ Mon, 12 Aug 2019 22:40:08: 25000000 INFO @ Mon, 12 Aug 2019 22:40:12: 25000000 INFO @ Mon, 12 Aug 2019 22:40:12: 25000000 INFO @ Mon, 12 Aug 2019 22:40:15: 26000000 INFO @ Mon, 12 Aug 2019 22:40:18: 26000000 INFO @ Mon, 12 Aug 2019 22:40:19: 26000000 INFO @ Mon, 12 Aug 2019 22:40:21: 27000000 INFO @ Mon, 12 Aug 2019 22:40:25: 27000000 INFO @ Mon, 12 Aug 2019 22:40:25: 27000000 INFO @ Mon, 12 Aug 2019 22:40:28: 28000000 INFO @ Mon, 12 Aug 2019 22:40:32: 28000000 INFO @ Mon, 12 Aug 2019 22:40:33: 28000000 INFO @ Mon, 12 Aug 2019 22:40:35: 29000000 INFO @ Mon, 12 Aug 2019 22:40:40: 29000000 INFO @ Mon, 12 Aug 2019 22:40:40: 29000000 INFO @ Mon, 12 Aug 2019 22:40:43: 30000000 INFO @ Mon, 12 Aug 2019 22:40:47: 30000000 INFO @ Mon, 12 Aug 2019 22:40:48: 30000000 INFO @ Mon, 12 Aug 2019 22:40:50: 31000000 INFO @ Mon, 12 Aug 2019 22:40:55: 31000000 INFO @ Mon, 12 Aug 2019 22:40:55: 31000000 INFO @ Mon, 12 Aug 2019 22:40:57: 32000000 INFO @ Mon, 12 Aug 2019 22:41:02: 32000000 INFO @ Mon, 12 Aug 2019 22:41:02: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:41:02: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:41:02: #1 total tags in treatment: 32734696 INFO @ Mon, 12 Aug 2019 22:41:02: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:41:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:41:03: 32000000 INFO @ Mon, 12 Aug 2019 22:41:03: #1 tags after filtering in treatment: 32734696 INFO @ Mon, 12 Aug 2019 22:41:03: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:41:03: #1 finished! INFO @ Mon, 12 Aug 2019 22:41:03: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:41:03: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:41:06: #2 number of paired peaks: 19 WARNING @ Mon, 12 Aug 2019 22:41:06: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:41:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981725/SRX3981725.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981725/SRX3981725.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981725/SRX3981725.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981725/SRX3981725.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 22:41:08: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:41:08: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:41:08: #1 total tags in treatment: 32734696 INFO @ Mon, 12 Aug 2019 22:41:08: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:41:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:41:08: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:41:08: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:41:08: #1 total tags in treatment: 32734696 INFO @ Mon, 12 Aug 2019 22:41:08: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:41:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:41:09: #1 tags after filtering in treatment: 32734696 INFO @ Mon, 12 Aug 2019 22:41:09: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:41:09: #1 finished! INFO @ Mon, 12 Aug 2019 22:41:09: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:41:09: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:41:09: #1 tags after filtering in treatment: 32734696 INFO @ Mon, 12 Aug 2019 22:41:09: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:41:09: #1 finished! INFO @ Mon, 12 Aug 2019 22:41:09: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:41:09: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:41:11: #2 number of paired peaks: 19 WARNING @ Mon, 12 Aug 2019 22:41:11: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:41:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981725/SRX3981725.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981725/SRX3981725.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981725/SRX3981725.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981725/SRX3981725.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 22:41:12: #2 number of paired peaks: 19 WARNING @ Mon, 12 Aug 2019 22:41:12: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:41:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981725/SRX3981725.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981725/SRX3981725.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981725/SRX3981725.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981725/SRX3981725.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。