Job ID = 2590497 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 13,219,148 reads read : 26,438,296 reads written : 13,219,148 reads 0-length : 13,219,148 spots read : 13,379,296 reads read : 26,758,592 reads written : 13,379,296 reads 0-length : 13,379,296 spots read : 13,460,492 reads read : 26,920,984 reads written : 13,460,492 reads 0-length : 13,460,492 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:49 40058936 reads; of these: 40058936 (100.00%) were unpaired; of these: 1121989 (2.80%) aligned 0 times 28039690 (70.00%) aligned exactly 1 time 10897257 (27.20%) aligned >1 times 97.20% overall alignment rate Time searching: 00:14:49 Overall time: 00:14:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4502315 / 38936947 = 0.1156 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 22:38:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981724/SRX3981724.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981724/SRX3981724.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981724/SRX3981724.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981724/SRX3981724.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:38:31: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:38:31: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:38:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981724/SRX3981724.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981724/SRX3981724.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981724/SRX3981724.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981724/SRX3981724.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:38:32: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:38:32: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:38:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981724/SRX3981724.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981724/SRX3981724.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981724/SRX3981724.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981724/SRX3981724.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:38:33: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:38:33: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:38:40: 1000000 INFO @ Mon, 12 Aug 2019 22:38:41: 1000000 INFO @ Mon, 12 Aug 2019 22:38:41: 1000000 INFO @ Mon, 12 Aug 2019 22:38:48: 2000000 INFO @ Mon, 12 Aug 2019 22:38:48: 2000000 INFO @ Mon, 12 Aug 2019 22:38:49: 2000000 INFO @ Mon, 12 Aug 2019 22:38:55: 3000000 INFO @ Mon, 12 Aug 2019 22:38:57: 3000000 INFO @ Mon, 12 Aug 2019 22:38:57: 3000000 INFO @ Mon, 12 Aug 2019 22:39:03: 4000000 INFO @ Mon, 12 Aug 2019 22:39:05: 4000000 INFO @ Mon, 12 Aug 2019 22:39:06: 4000000 INFO @ Mon, 12 Aug 2019 22:39:10: 5000000 INFO @ Mon, 12 Aug 2019 22:39:14: 5000000 INFO @ Mon, 12 Aug 2019 22:39:14: 5000000 INFO @ Mon, 12 Aug 2019 22:39:17: 6000000 INFO @ Mon, 12 Aug 2019 22:39:22: 6000000 INFO @ Mon, 12 Aug 2019 22:39:23: 6000000 INFO @ Mon, 12 Aug 2019 22:39:24: 7000000 INFO @ Mon, 12 Aug 2019 22:39:31: 7000000 INFO @ Mon, 12 Aug 2019 22:39:31: 7000000 INFO @ Mon, 12 Aug 2019 22:39:32: 8000000 INFO @ Mon, 12 Aug 2019 22:39:39: 9000000 INFO @ Mon, 12 Aug 2019 22:39:39: 8000000 INFO @ Mon, 12 Aug 2019 22:39:39: 8000000 INFO @ Mon, 12 Aug 2019 22:39:46: 10000000 INFO @ Mon, 12 Aug 2019 22:39:48: 9000000 INFO @ Mon, 12 Aug 2019 22:39:48: 9000000 INFO @ Mon, 12 Aug 2019 22:39:54: 11000000 INFO @ Mon, 12 Aug 2019 22:39:57: 10000000 INFO @ Mon, 12 Aug 2019 22:39:57: 10000000 INFO @ Mon, 12 Aug 2019 22:40:02: 12000000 INFO @ Mon, 12 Aug 2019 22:40:06: 11000000 INFO @ Mon, 12 Aug 2019 22:40:06: 11000000 INFO @ Mon, 12 Aug 2019 22:40:10: 13000000 INFO @ Mon, 12 Aug 2019 22:40:16: 12000000 INFO @ Mon, 12 Aug 2019 22:40:16: 12000000 INFO @ Mon, 12 Aug 2019 22:40:18: 14000000 INFO @ Mon, 12 Aug 2019 22:40:25: 13000000 INFO @ Mon, 12 Aug 2019 22:40:25: 15000000 INFO @ Mon, 12 Aug 2019 22:40:26: 13000000 INFO @ Mon, 12 Aug 2019 22:40:33: 16000000 INFO @ Mon, 12 Aug 2019 22:40:34: 14000000 INFO @ Mon, 12 Aug 2019 22:40:35: 14000000 INFO @ Mon, 12 Aug 2019 22:40:41: 17000000 INFO @ Mon, 12 Aug 2019 22:40:43: 15000000 INFO @ Mon, 12 Aug 2019 22:40:44: 15000000 INFO @ Mon, 12 Aug 2019 22:40:48: 18000000 INFO @ Mon, 12 Aug 2019 22:40:52: 16000000 INFO @ Mon, 12 Aug 2019 22:40:53: 16000000 INFO @ Mon, 12 Aug 2019 22:40:56: 19000000 INFO @ Mon, 12 Aug 2019 22:41:00: 17000000 INFO @ Mon, 12 Aug 2019 22:41:02: 17000000 INFO @ Mon, 12 Aug 2019 22:41:03: 20000000 INFO @ Mon, 12 Aug 2019 22:41:09: 18000000 INFO @ Mon, 12 Aug 2019 22:41:11: 18000000 INFO @ Mon, 12 Aug 2019 22:41:11: 21000000 INFO @ Mon, 12 Aug 2019 22:41:18: 19000000 INFO @ Mon, 12 Aug 2019 22:41:19: 22000000 INFO @ Mon, 12 Aug 2019 22:41:19: 19000000 INFO @ Mon, 12 Aug 2019 22:41:26: 23000000 INFO @ Mon, 12 Aug 2019 22:41:26: 20000000 INFO @ Mon, 12 Aug 2019 22:41:29: 20000000 INFO @ Mon, 12 Aug 2019 22:41:34: 24000000 INFO @ Mon, 12 Aug 2019 22:41:35: 21000000 INFO @ Mon, 12 Aug 2019 22:41:38: 21000000 INFO @ Mon, 12 Aug 2019 22:41:42: 25000000 INFO @ Mon, 12 Aug 2019 22:41:44: 22000000 INFO @ Mon, 12 Aug 2019 22:41:48: 22000000 INFO @ Mon, 12 Aug 2019 22:41:50: 26000000 INFO @ Mon, 12 Aug 2019 22:41:53: 23000000 INFO @ Mon, 12 Aug 2019 22:41:57: 23000000 INFO @ Mon, 12 Aug 2019 22:41:59: 27000000 INFO @ Mon, 12 Aug 2019 22:42:03: 24000000 INFO @ Mon, 12 Aug 2019 22:42:07: 24000000 INFO @ Mon, 12 Aug 2019 22:42:08: 28000000 INFO @ Mon, 12 Aug 2019 22:42:12: 25000000 INFO @ Mon, 12 Aug 2019 22:42:16: 25000000 INFO @ Mon, 12 Aug 2019 22:42:17: 29000000 INFO @ Mon, 12 Aug 2019 22:42:20: 26000000 INFO @ Mon, 12 Aug 2019 22:42:24: 30000000 INFO @ Mon, 12 Aug 2019 22:42:25: 26000000 INFO @ Mon, 12 Aug 2019 22:42:29: 27000000 INFO @ Mon, 12 Aug 2019 22:42:32: 31000000 INFO @ Mon, 12 Aug 2019 22:42:35: 27000000 INFO @ Mon, 12 Aug 2019 22:42:38: 28000000 INFO @ Mon, 12 Aug 2019 22:42:39: 32000000 INFO @ Mon, 12 Aug 2019 22:42:44: 28000000 INFO @ Mon, 12 Aug 2019 22:42:46: 33000000 INFO @ Mon, 12 Aug 2019 22:42:47: 29000000 INFO @ Mon, 12 Aug 2019 22:42:53: 34000000 INFO @ Mon, 12 Aug 2019 22:42:54: 29000000 INFO @ Mon, 12 Aug 2019 22:42:56: 30000000 INFO @ Mon, 12 Aug 2019 22:42:57: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:42:57: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:42:57: #1 total tags in treatment: 34434632 INFO @ Mon, 12 Aug 2019 22:42:57: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:42:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:42:58: #1 tags after filtering in treatment: 34434632 INFO @ Mon, 12 Aug 2019 22:42:58: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:42:58: #1 finished! INFO @ Mon, 12 Aug 2019 22:42:58: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:42:58: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:43:00: #2 number of paired peaks: 19 WARNING @ Mon, 12 Aug 2019 22:43:00: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:43:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981724/SRX3981724.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981724/SRX3981724.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981724/SRX3981724.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981724/SRX3981724.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 22:43:03: 30000000 INFO @ Mon, 12 Aug 2019 22:43:05: 31000000 INFO @ Mon, 12 Aug 2019 22:43:13: 31000000 INFO @ Mon, 12 Aug 2019 22:43:14: 32000000 INFO @ Mon, 12 Aug 2019 22:43:22: 32000000 INFO @ Mon, 12 Aug 2019 22:43:23: 33000000 INFO @ Mon, 12 Aug 2019 22:43:31: 33000000 INFO @ Mon, 12 Aug 2019 22:43:31: 34000000 INFO @ Mon, 12 Aug 2019 22:43:35: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:43:35: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:43:35: #1 total tags in treatment: 34434632 INFO @ Mon, 12 Aug 2019 22:43:35: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:43:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:43:36: #1 tags after filtering in treatment: 34434632 INFO @ Mon, 12 Aug 2019 22:43:36: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:43:36: #1 finished! INFO @ Mon, 12 Aug 2019 22:43:36: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:43:36: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:43:39: #2 number of paired peaks: 19 WARNING @ Mon, 12 Aug 2019 22:43:39: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:43:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981724/SRX3981724.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981724/SRX3981724.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981724/SRX3981724.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981724/SRX3981724.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 22:43:40: 34000000 INFO @ Mon, 12 Aug 2019 22:43:44: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:43:44: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:43:44: #1 total tags in treatment: 34434632 INFO @ Mon, 12 Aug 2019 22:43:44: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:43:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:43:45: #1 tags after filtering in treatment: 34434632 INFO @ Mon, 12 Aug 2019 22:43:45: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:43:45: #1 finished! INFO @ Mon, 12 Aug 2019 22:43:45: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:43:45: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:43:48: #2 number of paired peaks: 19 WARNING @ Mon, 12 Aug 2019 22:43:48: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:43:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981724/SRX3981724.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981724/SRX3981724.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981724/SRX3981724.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981724/SRX3981724.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。