Job ID = 6528070 SRX = SRX3981721 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:17:36 prefetch.2.10.7: 1) Downloading 'SRR7050575'... 2020-06-29T14:17:36 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:18:38 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:18:38 prefetch.2.10.7: 'SRR7050575' is valid 2020-06-29T14:18:38 prefetch.2.10.7: 1) 'SRR7050575' was downloaded successfully 2020-06-29T14:18:38 prefetch.2.10.7: 'SRR7050575' has 0 unresolved dependencies Read 12233321 spots for SRR7050575/SRR7050575.sra Written 12233321 spots for SRR7050575/SRR7050575.sra 2020-06-29T14:19:30 prefetch.2.10.7: 1) Downloading 'SRR7050576'... 2020-06-29T14:19:30 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:20:35 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:20:36 prefetch.2.10.7: 'SRR7050576' is valid 2020-06-29T14:20:36 prefetch.2.10.7: 1) 'SRR7050576' was downloaded successfully 2020-06-29T14:20:36 prefetch.2.10.7: 'SRR7050576' has 0 unresolved dependencies Read 12376641 spots for SRR7050576/SRR7050576.sra Written 12376641 spots for SRR7050576/SRR7050576.sra 2020-06-29T14:21:32 prefetch.2.10.7: 1) Downloading 'SRR7050577'... 2020-06-29T14:21:32 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:22:31 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:22:32 prefetch.2.10.7: 'SRR7050577' is valid 2020-06-29T14:22:32 prefetch.2.10.7: 1) 'SRR7050577' was downloaded successfully 2020-06-29T14:22:32 prefetch.2.10.7: 'SRR7050577' has 0 unresolved dependencies Read 12422194 spots for SRR7050577/SRR7050577.sra Written 12422194 spots for SRR7050577/SRR7050577.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:11 37032156 reads; of these: 37032156 (100.00%) were unpaired; of these: 1023919 (2.76%) aligned 0 times 25721753 (69.46%) aligned exactly 1 time 10286484 (27.78%) aligned >1 times 97.24% overall alignment rate Time searching: 00:12:11 Overall time: 00:12:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4116983 / 36008237 = 0.1143 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:52:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981721/SRX3981721.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981721/SRX3981721.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981721/SRX3981721.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981721/SRX3981721.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:52:11: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:52:11: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:52:16: 1000000 INFO @ Mon, 29 Jun 2020 23:52:21: 2000000 INFO @ Mon, 29 Jun 2020 23:52:26: 3000000 INFO @ Mon, 29 Jun 2020 23:52:31: 4000000 INFO @ Mon, 29 Jun 2020 23:52:36: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:52:40: 6000000 INFO @ Mon, 29 Jun 2020 23:52:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981721/SRX3981721.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981721/SRX3981721.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981721/SRX3981721.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981721/SRX3981721.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:52:41: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:52:41: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:52:45: 7000000 INFO @ Mon, 29 Jun 2020 23:52:46: 1000000 INFO @ Mon, 29 Jun 2020 23:52:51: 8000000 INFO @ Mon, 29 Jun 2020 23:52:51: 2000000 INFO @ Mon, 29 Jun 2020 23:52:56: 9000000 INFO @ Mon, 29 Jun 2020 23:52:56: 3000000 INFO @ Mon, 29 Jun 2020 23:53:01: 10000000 INFO @ Mon, 29 Jun 2020 23:53:01: 4000000 INFO @ Mon, 29 Jun 2020 23:53:06: 11000000 INFO @ Mon, 29 Jun 2020 23:53:06: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:53:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981721/SRX3981721.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981721/SRX3981721.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981721/SRX3981721.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981721/SRX3981721.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:53:11: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:53:11: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:53:11: 12000000 INFO @ Mon, 29 Jun 2020 23:53:11: 6000000 INFO @ Mon, 29 Jun 2020 23:53:16: 1000000 INFO @ Mon, 29 Jun 2020 23:53:16: 13000000 INFO @ Mon, 29 Jun 2020 23:53:16: 7000000 INFO @ Mon, 29 Jun 2020 23:53:21: 2000000 INFO @ Mon, 29 Jun 2020 23:53:22: 8000000 INFO @ Mon, 29 Jun 2020 23:53:22: 14000000 INFO @ Mon, 29 Jun 2020 23:53:26: 3000000 INFO @ Mon, 29 Jun 2020 23:53:27: 9000000 INFO @ Mon, 29 Jun 2020 23:53:27: 15000000 INFO @ Mon, 29 Jun 2020 23:53:32: 4000000 INFO @ Mon, 29 Jun 2020 23:53:32: 10000000 INFO @ Mon, 29 Jun 2020 23:53:32: 16000000 INFO @ Mon, 29 Jun 2020 23:53:37: 5000000 INFO @ Mon, 29 Jun 2020 23:53:37: 11000000 INFO @ Mon, 29 Jun 2020 23:53:38: 17000000 INFO @ Mon, 29 Jun 2020 23:53:42: 6000000 INFO @ Mon, 29 Jun 2020 23:53:42: 12000000 INFO @ Mon, 29 Jun 2020 23:53:43: 18000000 INFO @ Mon, 29 Jun 2020 23:53:47: 7000000 INFO @ Mon, 29 Jun 2020 23:53:48: 13000000 INFO @ Mon, 29 Jun 2020 23:53:48: 19000000 INFO @ Mon, 29 Jun 2020 23:53:52: 8000000 INFO @ Mon, 29 Jun 2020 23:53:53: 14000000 INFO @ Mon, 29 Jun 2020 23:53:53: 20000000 INFO @ Mon, 29 Jun 2020 23:53:57: 9000000 INFO @ Mon, 29 Jun 2020 23:53:58: 15000000 INFO @ Mon, 29 Jun 2020 23:53:58: 21000000 INFO @ Mon, 29 Jun 2020 23:54:02: 10000000 INFO @ Mon, 29 Jun 2020 23:54:03: 16000000 INFO @ Mon, 29 Jun 2020 23:54:03: 22000000 INFO @ Mon, 29 Jun 2020 23:54:08: 11000000 INFO @ Mon, 29 Jun 2020 23:54:09: 23000000 INFO @ Mon, 29 Jun 2020 23:54:09: 17000000 INFO @ Mon, 29 Jun 2020 23:54:13: 12000000 INFO @ Mon, 29 Jun 2020 23:54:14: 24000000 INFO @ Mon, 29 Jun 2020 23:54:14: 18000000 INFO @ Mon, 29 Jun 2020 23:54:18: 13000000 INFO @ Mon, 29 Jun 2020 23:54:19: 19000000 INFO @ Mon, 29 Jun 2020 23:54:19: 25000000 INFO @ Mon, 29 Jun 2020 23:54:23: 14000000 INFO @ Mon, 29 Jun 2020 23:54:24: 26000000 INFO @ Mon, 29 Jun 2020 23:54:24: 20000000 INFO @ Mon, 29 Jun 2020 23:54:28: 15000000 INFO @ Mon, 29 Jun 2020 23:54:29: 21000000 INFO @ Mon, 29 Jun 2020 23:54:29: 27000000 INFO @ Mon, 29 Jun 2020 23:54:33: 16000000 INFO @ Mon, 29 Jun 2020 23:54:34: 22000000 INFO @ Mon, 29 Jun 2020 23:54:35: 28000000 INFO @ Mon, 29 Jun 2020 23:54:38: 17000000 INFO @ Mon, 29 Jun 2020 23:54:39: 23000000 INFO @ Mon, 29 Jun 2020 23:54:40: 29000000 INFO @ Mon, 29 Jun 2020 23:54:43: 18000000 INFO @ Mon, 29 Jun 2020 23:54:44: 24000000 INFO @ Mon, 29 Jun 2020 23:54:45: 30000000 INFO @ Mon, 29 Jun 2020 23:54:48: 19000000 INFO @ Mon, 29 Jun 2020 23:54:50: 25000000 INFO @ Mon, 29 Jun 2020 23:54:50: 31000000 INFO @ Mon, 29 Jun 2020 23:54:54: 20000000 INFO @ Mon, 29 Jun 2020 23:54:55: 26000000 INFO @ Mon, 29 Jun 2020 23:54:56: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:54:56: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:54:56: #1 total tags in treatment: 31891254 INFO @ Mon, 29 Jun 2020 23:54:56: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:54:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:54:56: #1 tags after filtering in treatment: 31891254 INFO @ Mon, 29 Jun 2020 23:54:56: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:54:56: #1 finished! INFO @ Mon, 29 Jun 2020 23:54:56: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:54:56: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:54:58: #2 number of paired peaks: 26 WARNING @ Mon, 29 Jun 2020 23:54:58: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:54:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981721/SRX3981721.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981721/SRX3981721.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981721/SRX3981721.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981721/SRX3981721.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:54:59: 21000000 INFO @ Mon, 29 Jun 2020 23:55:00: 27000000 INFO @ Mon, 29 Jun 2020 23:55:04: 22000000 INFO @ Mon, 29 Jun 2020 23:55:05: 28000000 INFO @ Mon, 29 Jun 2020 23:55:09: 23000000 INFO @ Mon, 29 Jun 2020 23:55:11: 29000000 INFO @ Mon, 29 Jun 2020 23:55:14: 24000000 INFO @ Mon, 29 Jun 2020 23:55:16: 30000000 INFO @ Mon, 29 Jun 2020 23:55:19: 25000000 INFO @ Mon, 29 Jun 2020 23:55:21: 31000000 INFO @ Mon, 29 Jun 2020 23:55:24: 26000000 INFO @ Mon, 29 Jun 2020 23:55:26: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:55:26: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:55:26: #1 total tags in treatment: 31891254 INFO @ Mon, 29 Jun 2020 23:55:26: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:55:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:55:27: #1 tags after filtering in treatment: 31891254 INFO @ Mon, 29 Jun 2020 23:55:27: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:55:27: #1 finished! INFO @ Mon, 29 Jun 2020 23:55:27: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:55:27: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:55:29: #2 number of paired peaks: 26 WARNING @ Mon, 29 Jun 2020 23:55:29: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:55:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981721/SRX3981721.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981721/SRX3981721.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981721/SRX3981721.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981721/SRX3981721.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:55:30: 27000000 INFO @ Mon, 29 Jun 2020 23:55:35: 28000000 INFO @ Mon, 29 Jun 2020 23:55:40: 29000000 INFO @ Mon, 29 Jun 2020 23:55:45: 30000000 INFO @ Mon, 29 Jun 2020 23:55:50: 31000000 INFO @ Mon, 29 Jun 2020 23:55:55: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:55:55: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:55:55: #1 total tags in treatment: 31891254 INFO @ Mon, 29 Jun 2020 23:55:55: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:55:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:55:55: #1 tags after filtering in treatment: 31891254 INFO @ Mon, 29 Jun 2020 23:55:55: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:55:55: #1 finished! INFO @ Mon, 29 Jun 2020 23:55:55: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:55:55: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:55:57: #2 number of paired peaks: 26 WARNING @ Mon, 29 Jun 2020 23:55:57: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:55:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981721/SRX3981721.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981721/SRX3981721.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981721/SRX3981721.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981721/SRX3981721.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。