Job ID = 2590490 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 10,867,358 reads read : 21,734,716 reads written : 10,867,358 reads 0-length : 10,867,358 spots read : 10,988,930 reads read : 21,977,860 reads written : 10,988,930 reads 0-length : 10,988,930 spots read : 11,044,386 reads read : 22,088,772 reads written : 11,044,386 reads 0-length : 11,044,386 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:55 32900674 reads; of these: 32900674 (100.00%) were unpaired; of these: 897465 (2.73%) aligned 0 times 23029591 (70.00%) aligned exactly 1 time 8973618 (27.27%) aligned >1 times 97.27% overall alignment rate Time searching: 00:12:56 Overall time: 00:12:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 3414770 / 32003209 = 0.1067 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 22:28:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981717/SRX3981717.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981717/SRX3981717.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981717/SRX3981717.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981717/SRX3981717.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:28:27: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:28:27: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:28:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981717/SRX3981717.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981717/SRX3981717.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981717/SRX3981717.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981717/SRX3981717.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:28:29: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:28:29: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:28:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981717/SRX3981717.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981717/SRX3981717.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981717/SRX3981717.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981717/SRX3981717.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:28:30: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:28:30: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:28:36: 1000000 INFO @ Mon, 12 Aug 2019 22:28:37: 1000000 INFO @ Mon, 12 Aug 2019 22:28:37: 1000000 INFO @ Mon, 12 Aug 2019 22:28:43: 2000000 INFO @ Mon, 12 Aug 2019 22:28:44: 2000000 INFO @ Mon, 12 Aug 2019 22:28:45: 2000000 INFO @ Mon, 12 Aug 2019 22:28:50: 3000000 INFO @ Mon, 12 Aug 2019 22:28:51: 3000000 INFO @ Mon, 12 Aug 2019 22:28:53: 3000000 INFO @ Mon, 12 Aug 2019 22:28:56: 4000000 INFO @ Mon, 12 Aug 2019 22:28:59: 4000000 INFO @ Mon, 12 Aug 2019 22:29:01: 4000000 INFO @ Mon, 12 Aug 2019 22:29:03: 5000000 INFO @ Mon, 12 Aug 2019 22:29:06: 5000000 INFO @ Mon, 12 Aug 2019 22:29:09: 5000000 INFO @ Mon, 12 Aug 2019 22:29:09: 6000000 INFO @ Mon, 12 Aug 2019 22:29:13: 6000000 INFO @ Mon, 12 Aug 2019 22:29:16: 7000000 INFO @ Mon, 12 Aug 2019 22:29:17: 6000000 INFO @ Mon, 12 Aug 2019 22:29:20: 7000000 INFO @ Mon, 12 Aug 2019 22:29:23: 8000000 INFO @ Mon, 12 Aug 2019 22:29:24: 7000000 INFO @ Mon, 12 Aug 2019 22:29:27: 8000000 INFO @ Mon, 12 Aug 2019 22:29:29: 9000000 INFO @ Mon, 12 Aug 2019 22:29:31: 8000000 INFO @ Mon, 12 Aug 2019 22:29:34: 9000000 INFO @ Mon, 12 Aug 2019 22:29:36: 10000000 INFO @ Mon, 12 Aug 2019 22:29:38: 9000000 INFO @ Mon, 12 Aug 2019 22:29:42: 10000000 INFO @ Mon, 12 Aug 2019 22:29:43: 11000000 INFO @ Mon, 12 Aug 2019 22:29:46: 10000000 INFO @ Mon, 12 Aug 2019 22:29:50: 11000000 INFO @ Mon, 12 Aug 2019 22:29:50: 12000000 INFO @ Mon, 12 Aug 2019 22:29:53: 11000000 INFO @ Mon, 12 Aug 2019 22:29:56: 13000000 INFO @ Mon, 12 Aug 2019 22:29:57: 12000000 INFO @ Mon, 12 Aug 2019 22:30:01: 12000000 INFO @ Mon, 12 Aug 2019 22:30:03: 14000000 INFO @ Mon, 12 Aug 2019 22:30:05: 13000000 INFO @ Mon, 12 Aug 2019 22:30:09: 13000000 INFO @ Mon, 12 Aug 2019 22:30:10: 15000000 INFO @ Mon, 12 Aug 2019 22:30:12: 14000000 INFO @ Mon, 12 Aug 2019 22:30:16: 14000000 INFO @ Mon, 12 Aug 2019 22:30:17: 16000000 INFO @ Mon, 12 Aug 2019 22:30:20: 15000000 INFO @ Mon, 12 Aug 2019 22:30:23: 17000000 INFO @ Mon, 12 Aug 2019 22:30:23: 15000000 INFO @ Mon, 12 Aug 2019 22:30:27: 16000000 INFO @ Mon, 12 Aug 2019 22:30:30: 18000000 INFO @ Mon, 12 Aug 2019 22:30:31: 16000000 INFO @ Mon, 12 Aug 2019 22:30:34: 17000000 INFO @ Mon, 12 Aug 2019 22:30:37: 19000000 INFO @ Mon, 12 Aug 2019 22:30:38: 17000000 INFO @ Mon, 12 Aug 2019 22:30:42: 18000000 INFO @ Mon, 12 Aug 2019 22:30:43: 20000000 INFO @ Mon, 12 Aug 2019 22:30:45: 18000000 INFO @ Mon, 12 Aug 2019 22:30:49: 19000000 INFO @ Mon, 12 Aug 2019 22:30:50: 21000000 INFO @ Mon, 12 Aug 2019 22:30:53: 19000000 INFO @ Mon, 12 Aug 2019 22:30:56: 20000000 INFO @ Mon, 12 Aug 2019 22:30:57: 22000000 INFO @ Mon, 12 Aug 2019 22:31:00: 20000000 INFO @ Mon, 12 Aug 2019 22:31:03: 23000000 INFO @ Mon, 12 Aug 2019 22:31:03: 21000000 INFO @ Mon, 12 Aug 2019 22:31:07: 21000000 INFO @ Mon, 12 Aug 2019 22:31:10: 24000000 INFO @ Mon, 12 Aug 2019 22:31:11: 22000000 INFO @ Mon, 12 Aug 2019 22:31:14: 22000000 INFO @ Mon, 12 Aug 2019 22:31:17: 25000000 INFO @ Mon, 12 Aug 2019 22:31:18: 23000000 INFO @ Mon, 12 Aug 2019 22:31:22: 23000000 INFO @ Mon, 12 Aug 2019 22:31:23: 26000000 INFO @ Mon, 12 Aug 2019 22:31:25: 24000000 INFO @ Mon, 12 Aug 2019 22:31:29: 24000000 INFO @ Mon, 12 Aug 2019 22:31:30: 27000000 INFO @ Mon, 12 Aug 2019 22:31:32: 25000000 INFO @ Mon, 12 Aug 2019 22:31:36: 25000000 INFO @ Mon, 12 Aug 2019 22:31:36: 28000000 INFO @ Mon, 12 Aug 2019 22:31:39: 26000000 INFO @ Mon, 12 Aug 2019 22:31:41: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:31:41: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:31:41: #1 total tags in treatment: 28588439 INFO @ Mon, 12 Aug 2019 22:31:41: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:31:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:31:41: #1 tags after filtering in treatment: 28588439 INFO @ Mon, 12 Aug 2019 22:31:41: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:31:41: #1 finished! INFO @ Mon, 12 Aug 2019 22:31:41: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:31:41: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:31:43: 26000000 INFO @ Mon, 12 Aug 2019 22:31:43: #2 number of paired peaks: 39 WARNING @ Mon, 12 Aug 2019 22:31:43: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:31:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981717/SRX3981717.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981717/SRX3981717.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981717/SRX3981717.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981717/SRX3981717.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 22:31:46: 27000000 INFO @ Mon, 12 Aug 2019 22:31:50: 27000000 INFO @ Mon, 12 Aug 2019 22:31:54: 28000000 INFO @ Mon, 12 Aug 2019 22:31:57: 28000000 INFO @ Mon, 12 Aug 2019 22:31:58: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:31:58: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:31:58: #1 total tags in treatment: 28588439 INFO @ Mon, 12 Aug 2019 22:31:58: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:31:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:31:59: #1 tags after filtering in treatment: 28588439 INFO @ Mon, 12 Aug 2019 22:31:59: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:31:59: #1 finished! INFO @ Mon, 12 Aug 2019 22:31:59: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:31:59: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:32:01: #2 number of paired peaks: 39 WARNING @ Mon, 12 Aug 2019 22:32:01: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:32:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981717/SRX3981717.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981717/SRX3981717.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981717/SRX3981717.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981717/SRX3981717.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 22:32:02: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:32:02: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:32:02: #1 total tags in treatment: 28588439 INFO @ Mon, 12 Aug 2019 22:32:02: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:32:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:32:02: #1 tags after filtering in treatment: 28588439 INFO @ Mon, 12 Aug 2019 22:32:02: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:32:02: #1 finished! INFO @ Mon, 12 Aug 2019 22:32:02: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:32:02: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:32:05: #2 number of paired peaks: 39 WARNING @ Mon, 12 Aug 2019 22:32:05: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:32:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981717/SRX3981717.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981717/SRX3981717.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981717/SRX3981717.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981717/SRX3981717.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。