Job ID = 6528066 SRX = SRX3981704 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T15:06:40 prefetch.2.10.7: 1) Downloading 'SRR7050511'... 2020-06-29T15:06:40 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:07:28 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:07:28 prefetch.2.10.7: 'SRR7050511' is valid 2020-06-29T15:07:28 prefetch.2.10.7: 1) 'SRR7050511' was downloaded successfully 2020-06-29T15:07:28 prefetch.2.10.7: 'SRR7050511' has 0 unresolved dependencies Read 7154824 spots for SRR7050511/SRR7050511.sra Written 7154824 spots for SRR7050511/SRR7050511.sra 2020-06-29T15:08:09 prefetch.2.10.7: 1) Downloading 'SRR7050512'... 2020-06-29T15:08:09 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:08:53 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:08:53 prefetch.2.10.7: 'SRR7050512' is valid 2020-06-29T15:08:53 prefetch.2.10.7: 1) 'SRR7050512' was downloaded successfully 2020-06-29T15:08:53 prefetch.2.10.7: 'SRR7050512' has 0 unresolved dependencies Read 7145223 spots for SRR7050512/SRR7050512.sra Written 7145223 spots for SRR7050512/SRR7050512.sra 2020-06-29T15:09:34 prefetch.2.10.7: 1) Downloading 'SRR7050513'... 2020-06-29T15:09:34 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:10:37 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:10:38 prefetch.2.10.7: 'SRR7050513' is valid 2020-06-29T15:10:38 prefetch.2.10.7: 1) 'SRR7050513' was downloaded successfully 2020-06-29T15:10:38 prefetch.2.10.7: 'SRR7050513' has 0 unresolved dependencies Read 7176269 spots for SRR7050513/SRR7050513.sra Written 7176269 spots for SRR7050513/SRR7050513.sra 2020-06-29T15:11:18 prefetch.2.10.7: 1) Downloading 'SRR7050514'... 2020-06-29T15:11:18 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T15:12:07 prefetch.2.10.7: HTTPS download succeed 2020-06-29T15:12:08 prefetch.2.10.7: 'SRR7050514' is valid 2020-06-29T15:12:08 prefetch.2.10.7: 1) 'SRR7050514' was downloaded successfully 2020-06-29T15:12:08 prefetch.2.10.7: 'SRR7050514' has 0 unresolved dependencies Read 7215143 spots for SRR7050514/SRR7050514.sra Written 7215143 spots for SRR7050514/SRR7050514.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:05 28691459 reads; of these: 28691459 (100.00%) were unpaired; of these: 1524619 (5.31%) aligned 0 times 19428893 (67.72%) aligned exactly 1 time 7737947 (26.97%) aligned >1 times 94.69% overall alignment rate Time searching: 00:10:05 Overall time: 00:10:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 3806984 / 27166840 = 0.1401 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:37:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981704/SRX3981704.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981704/SRX3981704.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981704/SRX3981704.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981704/SRX3981704.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:37:45: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:37:45: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:37:52: 1000000 INFO @ Tue, 30 Jun 2020 00:37:59: 2000000 INFO @ Tue, 30 Jun 2020 00:38:05: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:38:13: 4000000 INFO @ Tue, 30 Jun 2020 00:38:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981704/SRX3981704.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981704/SRX3981704.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981704/SRX3981704.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981704/SRX3981704.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:38:15: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:38:15: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:38:19: 5000000 INFO @ Tue, 30 Jun 2020 00:38:22: 1000000 INFO @ Tue, 30 Jun 2020 00:38:25: 6000000 INFO @ Tue, 30 Jun 2020 00:38:30: 2000000 INFO @ Tue, 30 Jun 2020 00:38:31: 7000000 INFO @ Tue, 30 Jun 2020 00:38:38: 8000000 INFO @ Tue, 30 Jun 2020 00:38:38: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:38:44: 9000000 INFO @ Tue, 30 Jun 2020 00:38:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981704/SRX3981704.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981704/SRX3981704.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981704/SRX3981704.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981704/SRX3981704.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:38:45: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:38:45: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:38:46: 4000000 INFO @ Tue, 30 Jun 2020 00:38:50: 10000000 INFO @ Tue, 30 Jun 2020 00:38:52: 1000000 INFO @ Tue, 30 Jun 2020 00:38:54: 5000000 INFO @ Tue, 30 Jun 2020 00:38:57: 11000000 INFO @ Tue, 30 Jun 2020 00:39:00: 2000000 INFO @ Tue, 30 Jun 2020 00:39:02: 6000000 INFO @ Tue, 30 Jun 2020 00:39:04: 12000000 INFO @ Tue, 30 Jun 2020 00:39:07: 3000000 INFO @ Tue, 30 Jun 2020 00:39:10: 7000000 INFO @ Tue, 30 Jun 2020 00:39:11: 13000000 INFO @ Tue, 30 Jun 2020 00:39:14: 4000000 INFO @ Tue, 30 Jun 2020 00:39:18: 8000000 INFO @ Tue, 30 Jun 2020 00:39:18: 14000000 INFO @ Tue, 30 Jun 2020 00:39:21: 5000000 INFO @ Tue, 30 Jun 2020 00:39:25: 15000000 INFO @ Tue, 30 Jun 2020 00:39:25: 9000000 INFO @ Tue, 30 Jun 2020 00:39:28: 6000000 INFO @ Tue, 30 Jun 2020 00:39:32: 16000000 INFO @ Tue, 30 Jun 2020 00:39:33: 10000000 INFO @ Tue, 30 Jun 2020 00:39:35: 7000000 INFO @ Tue, 30 Jun 2020 00:39:39: 17000000 INFO @ Tue, 30 Jun 2020 00:39:41: 11000000 INFO @ Tue, 30 Jun 2020 00:39:42: 8000000 INFO @ Tue, 30 Jun 2020 00:39:46: 18000000 INFO @ Tue, 30 Jun 2020 00:39:49: 12000000 INFO @ Tue, 30 Jun 2020 00:39:49: 9000000 INFO @ Tue, 30 Jun 2020 00:39:54: 19000000 INFO @ Tue, 30 Jun 2020 00:39:56: 13000000 INFO @ Tue, 30 Jun 2020 00:39:56: 10000000 INFO @ Tue, 30 Jun 2020 00:40:01: 20000000 INFO @ Tue, 30 Jun 2020 00:40:03: 11000000 INFO @ Tue, 30 Jun 2020 00:40:04: 14000000 INFO @ Tue, 30 Jun 2020 00:40:08: 21000000 INFO @ Tue, 30 Jun 2020 00:40:10: 12000000 INFO @ Tue, 30 Jun 2020 00:40:12: 15000000 INFO @ Tue, 30 Jun 2020 00:40:15: 22000000 INFO @ Tue, 30 Jun 2020 00:40:17: 13000000 INFO @ Tue, 30 Jun 2020 00:40:20: 16000000 INFO @ Tue, 30 Jun 2020 00:40:22: 23000000 INFO @ Tue, 30 Jun 2020 00:40:24: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:40:24: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:40:24: #1 total tags in treatment: 23359856 INFO @ Tue, 30 Jun 2020 00:40:24: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:40:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:40:24: 14000000 INFO @ Tue, 30 Jun 2020 00:40:25: #1 tags after filtering in treatment: 23359856 INFO @ Tue, 30 Jun 2020 00:40:25: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:40:25: #1 finished! INFO @ Tue, 30 Jun 2020 00:40:25: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:40:25: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:40:26: #2 number of paired peaks: 73 WARNING @ Tue, 30 Jun 2020 00:40:26: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:40:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981704/SRX3981704.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981704/SRX3981704.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981704/SRX3981704.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981704/SRX3981704.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:40:28: 17000000 INFO @ Tue, 30 Jun 2020 00:40:30: 15000000 INFO @ Tue, 30 Jun 2020 00:40:36: 18000000 INFO @ Tue, 30 Jun 2020 00:40:37: 16000000 INFO @ Tue, 30 Jun 2020 00:40:43: 19000000 INFO @ Tue, 30 Jun 2020 00:40:43: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:40:50: 18000000 INFO @ Tue, 30 Jun 2020 00:40:51: 20000000 INFO @ Tue, 30 Jun 2020 00:40:56: 19000000 INFO @ Tue, 30 Jun 2020 00:40:59: 21000000 INFO @ Tue, 30 Jun 2020 00:41:03: 20000000 INFO @ Tue, 30 Jun 2020 00:41:07: 22000000 INFO @ Tue, 30 Jun 2020 00:41:09: 21000000 INFO @ Tue, 30 Jun 2020 00:41:14: 23000000 INFO @ Tue, 30 Jun 2020 00:41:16: 22000000 INFO @ Tue, 30 Jun 2020 00:41:17: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:41:17: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:41:17: #1 total tags in treatment: 23359856 INFO @ Tue, 30 Jun 2020 00:41:17: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:41:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:41:18: #1 tags after filtering in treatment: 23359856 INFO @ Tue, 30 Jun 2020 00:41:18: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:41:18: #1 finished! INFO @ Tue, 30 Jun 2020 00:41:18: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:41:18: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:41:19: #2 number of paired peaks: 73 WARNING @ Tue, 30 Jun 2020 00:41:19: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:41:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981704/SRX3981704.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981704/SRX3981704.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981704/SRX3981704.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981704/SRX3981704.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:41:22: 23000000 INFO @ Tue, 30 Jun 2020 00:41:24: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:41:24: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:41:24: #1 total tags in treatment: 23359856 INFO @ Tue, 30 Jun 2020 00:41:24: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:41:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:41:24: #1 tags after filtering in treatment: 23359856 INFO @ Tue, 30 Jun 2020 00:41:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:41:24: #1 finished! INFO @ Tue, 30 Jun 2020 00:41:24: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:41:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:41:26: #2 number of paired peaks: 73 WARNING @ Tue, 30 Jun 2020 00:41:26: Too few paired peaks (73) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:41:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981704/SRX3981704.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981704/SRX3981704.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981704/SRX3981704.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981704/SRX3981704.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。