Job ID = 6528064 SRX = SRX3981700 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:43:58 prefetch.2.10.7: 1) Downloading 'SRR7050495'... 2020-06-29T14:43:58 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:44:49 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:44:49 prefetch.2.10.7: 'SRR7050495' is valid 2020-06-29T14:44:49 prefetch.2.10.7: 1) 'SRR7050495' was downloaded successfully 2020-06-29T14:44:49 prefetch.2.10.7: 'SRR7050495' has 0 unresolved dependencies Read 9005633 spots for SRR7050495/SRR7050495.sra Written 9005633 spots for SRR7050495/SRR7050495.sra 2020-06-29T14:45:37 prefetch.2.10.7: 1) Downloading 'SRR7050496'... 2020-06-29T14:45:37 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:46:19 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:46:20 prefetch.2.10.7: 'SRR7050496' is valid 2020-06-29T14:46:20 prefetch.2.10.7: 1) 'SRR7050496' was downloaded successfully 2020-06-29T14:46:20 prefetch.2.10.7: 'SRR7050496' has 0 unresolved dependencies Read 9003806 spots for SRR7050496/SRR7050496.sra Written 9003806 spots for SRR7050496/SRR7050496.sra 2020-06-29T14:47:07 prefetch.2.10.7: 1) Downloading 'SRR7050497'... 2020-06-29T14:47:07 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:47:58 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:47:58 prefetch.2.10.7: 'SRR7050497' is valid 2020-06-29T14:47:58 prefetch.2.10.7: 1) 'SRR7050497' was downloaded successfully 2020-06-29T14:47:58 prefetch.2.10.7: 'SRR7050497' has 0 unresolved dependencies Read 9042460 spots for SRR7050497/SRR7050497.sra Written 9042460 spots for SRR7050497/SRR7050497.sra 2020-06-29T14:48:46 prefetch.2.10.7: 1) Downloading 'SRR7050498'... 2020-06-29T14:48:46 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:49:32 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:49:33 prefetch.2.10.7: 'SRR7050498' is valid 2020-06-29T14:49:33 prefetch.2.10.7: 1) 'SRR7050498' was downloaded successfully 2020-06-29T14:49:33 prefetch.2.10.7: 'SRR7050498' has 0 unresolved dependencies Read 9072815 spots for SRR7050498/SRR7050498.sra Written 9072815 spots for SRR7050498/SRR7050498.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:17 36124714 reads; of these: 36124714 (100.00%) were unpaired; of these: 1081362 (2.99%) aligned 0 times 26401229 (73.08%) aligned exactly 1 time 8642123 (23.92%) aligned >1 times 97.01% overall alignment rate Time searching: 00:11:18 Overall time: 00:11:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4116869 / 35043352 = 0.1175 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:20:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981700/SRX3981700.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981700/SRX3981700.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981700/SRX3981700.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981700/SRX3981700.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:20:52: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:20:52: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:20:58: 1000000 INFO @ Tue, 30 Jun 2020 00:21:04: 2000000 INFO @ Tue, 30 Jun 2020 00:21:10: 3000000 INFO @ Tue, 30 Jun 2020 00:21:16: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:21:21: 5000000 INFO @ Tue, 30 Jun 2020 00:21:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981700/SRX3981700.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981700/SRX3981700.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981700/SRX3981700.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981700/SRX3981700.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:21:22: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:21:22: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:21:27: 6000000 INFO @ Tue, 30 Jun 2020 00:21:28: 1000000 INFO @ Tue, 30 Jun 2020 00:21:33: 7000000 INFO @ Tue, 30 Jun 2020 00:21:34: 2000000 INFO @ Tue, 30 Jun 2020 00:21:39: 8000000 INFO @ Tue, 30 Jun 2020 00:21:39: 3000000 INFO @ Tue, 30 Jun 2020 00:21:44: 4000000 INFO @ Tue, 30 Jun 2020 00:21:45: 9000000 INFO @ Tue, 30 Jun 2020 00:21:50: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:21:50: 10000000 INFO @ Tue, 30 Jun 2020 00:21:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981700/SRX3981700.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981700/SRX3981700.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981700/SRX3981700.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981700/SRX3981700.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:21:52: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:21:52: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:21:55: 6000000 INFO @ Tue, 30 Jun 2020 00:21:56: 11000000 INFO @ Tue, 30 Jun 2020 00:21:59: 1000000 INFO @ Tue, 30 Jun 2020 00:22:01: 7000000 INFO @ Tue, 30 Jun 2020 00:22:02: 12000000 INFO @ Tue, 30 Jun 2020 00:22:05: 2000000 INFO @ Tue, 30 Jun 2020 00:22:07: 8000000 INFO @ Tue, 30 Jun 2020 00:22:08: 13000000 INFO @ Tue, 30 Jun 2020 00:22:11: 3000000 INFO @ Tue, 30 Jun 2020 00:22:13: 9000000 INFO @ Tue, 30 Jun 2020 00:22:14: 14000000 INFO @ Tue, 30 Jun 2020 00:22:16: 4000000 INFO @ Tue, 30 Jun 2020 00:22:18: 10000000 INFO @ Tue, 30 Jun 2020 00:22:20: 15000000 INFO @ Tue, 30 Jun 2020 00:22:22: 5000000 INFO @ Tue, 30 Jun 2020 00:22:24: 11000000 INFO @ Tue, 30 Jun 2020 00:22:26: 16000000 INFO @ Tue, 30 Jun 2020 00:22:28: 6000000 INFO @ Tue, 30 Jun 2020 00:22:30: 12000000 INFO @ Tue, 30 Jun 2020 00:22:32: 17000000 INFO @ Tue, 30 Jun 2020 00:22:34: 7000000 INFO @ Tue, 30 Jun 2020 00:22:36: 13000000 INFO @ Tue, 30 Jun 2020 00:22:37: 18000000 INFO @ Tue, 30 Jun 2020 00:22:39: 8000000 INFO @ Tue, 30 Jun 2020 00:22:41: 14000000 INFO @ Tue, 30 Jun 2020 00:22:43: 19000000 INFO @ Tue, 30 Jun 2020 00:22:45: 9000000 INFO @ Tue, 30 Jun 2020 00:22:47: 15000000 INFO @ Tue, 30 Jun 2020 00:22:48: 20000000 INFO @ Tue, 30 Jun 2020 00:22:51: 10000000 INFO @ Tue, 30 Jun 2020 00:22:53: 16000000 INFO @ Tue, 30 Jun 2020 00:22:54: 21000000 INFO @ Tue, 30 Jun 2020 00:22:56: 11000000 INFO @ Tue, 30 Jun 2020 00:22:58: 17000000 INFO @ Tue, 30 Jun 2020 00:23:00: 22000000 INFO @ Tue, 30 Jun 2020 00:23:02: 12000000 INFO @ Tue, 30 Jun 2020 00:23:04: 18000000 INFO @ Tue, 30 Jun 2020 00:23:05: 23000000 INFO @ Tue, 30 Jun 2020 00:23:08: 13000000 INFO @ Tue, 30 Jun 2020 00:23:09: 19000000 INFO @ Tue, 30 Jun 2020 00:23:11: 24000000 INFO @ Tue, 30 Jun 2020 00:23:14: 14000000 INFO @ Tue, 30 Jun 2020 00:23:15: 20000000 INFO @ Tue, 30 Jun 2020 00:23:17: 25000000 INFO @ Tue, 30 Jun 2020 00:23:19: 15000000 INFO @ Tue, 30 Jun 2020 00:23:21: 21000000 INFO @ Tue, 30 Jun 2020 00:23:22: 26000000 INFO @ Tue, 30 Jun 2020 00:23:25: 16000000 INFO @ Tue, 30 Jun 2020 00:23:27: 22000000 INFO @ Tue, 30 Jun 2020 00:23:28: 27000000 INFO @ Tue, 30 Jun 2020 00:23:30: 17000000 INFO @ Tue, 30 Jun 2020 00:23:32: 23000000 INFO @ Tue, 30 Jun 2020 00:23:34: 28000000 INFO @ Tue, 30 Jun 2020 00:23:36: 18000000 INFO @ Tue, 30 Jun 2020 00:23:38: 24000000 INFO @ Tue, 30 Jun 2020 00:23:39: 29000000 INFO @ Tue, 30 Jun 2020 00:23:42: 19000000 INFO @ Tue, 30 Jun 2020 00:23:44: 25000000 INFO @ Tue, 30 Jun 2020 00:23:45: 30000000 INFO @ Tue, 30 Jun 2020 00:23:47: 20000000 INFO @ Tue, 30 Jun 2020 00:23:50: 26000000 INFO @ Tue, 30 Jun 2020 00:23:50: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:23:50: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:23:50: #1 total tags in treatment: 30926483 INFO @ Tue, 30 Jun 2020 00:23:50: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:23:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:23:51: #1 tags after filtering in treatment: 30926483 INFO @ Tue, 30 Jun 2020 00:23:51: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:23:51: #1 finished! INFO @ Tue, 30 Jun 2020 00:23:51: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:23:51: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:23:53: #2 number of paired peaks: 58 WARNING @ Tue, 30 Jun 2020 00:23:53: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:23:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981700/SRX3981700.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981700/SRX3981700.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981700/SRX3981700.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981700/SRX3981700.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:23:53: 21000000 INFO @ Tue, 30 Jun 2020 00:23:56: 27000000 INFO @ Tue, 30 Jun 2020 00:23:59: 22000000 INFO @ Tue, 30 Jun 2020 00:24:01: 28000000 INFO @ Tue, 30 Jun 2020 00:24:04: 23000000 INFO @ Tue, 30 Jun 2020 00:24:08: 29000000 INFO @ Tue, 30 Jun 2020 00:24:10: 24000000 INFO @ Tue, 30 Jun 2020 00:24:14: 30000000 INFO @ Tue, 30 Jun 2020 00:24:15: 25000000 INFO @ Tue, 30 Jun 2020 00:24:19: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:24:19: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:24:19: #1 total tags in treatment: 30926483 INFO @ Tue, 30 Jun 2020 00:24:19: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:24:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:24:20: #1 tags after filtering in treatment: 30926483 INFO @ Tue, 30 Jun 2020 00:24:20: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:24:20: #1 finished! INFO @ Tue, 30 Jun 2020 00:24:20: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:24:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:24:20: 26000000 INFO @ Tue, 30 Jun 2020 00:24:22: #2 number of paired peaks: 58 WARNING @ Tue, 30 Jun 2020 00:24:22: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:24:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981700/SRX3981700.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981700/SRX3981700.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981700/SRX3981700.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981700/SRX3981700.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:24:25: 27000000 INFO @ Tue, 30 Jun 2020 00:24:31: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:24:36: 29000000 INFO @ Tue, 30 Jun 2020 00:24:41: 30000000 INFO @ Tue, 30 Jun 2020 00:24:46: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:24:46: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:24:46: #1 total tags in treatment: 30926483 INFO @ Tue, 30 Jun 2020 00:24:46: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:24:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:24:46: #1 tags after filtering in treatment: 30926483 INFO @ Tue, 30 Jun 2020 00:24:46: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:24:46: #1 finished! INFO @ Tue, 30 Jun 2020 00:24:46: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:24:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:24:48: #2 number of paired peaks: 58 WARNING @ Tue, 30 Jun 2020 00:24:48: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:24:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981700/SRX3981700.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981700/SRX3981700.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981700/SRX3981700.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981700/SRX3981700.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。