Job ID = 2590463 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 11,240,282 reads read : 22,480,564 reads written : 11,240,282 reads 0-length : 11,240,282 spots read : 11,232,943 reads read : 22,465,886 reads written : 11,232,943 reads 0-length : 11,232,943 spots read : 11,272,778 reads read : 22,545,556 reads written : 11,272,778 reads 0-length : 11,272,778 spots read : 11,319,925 reads read : 22,639,850 reads written : 11,319,925 reads 0-length : 11,319,925 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:05 45065928 reads; of these: 45065928 (100.00%) were unpaired; of these: 1434898 (3.18%) aligned 0 times 31962220 (70.92%) aligned exactly 1 time 11668810 (25.89%) aligned >1 times 96.82% overall alignment rate Time searching: 00:17:05 Overall time: 00:17:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 5688517 / 43631030 = 0.1304 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 22:29:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981694/SRX3981694.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981694/SRX3981694.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981694/SRX3981694.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981694/SRX3981694.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:29:09: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:29:09: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:29:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981694/SRX3981694.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981694/SRX3981694.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981694/SRX3981694.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981694/SRX3981694.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:29:10: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:29:10: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:29:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981694/SRX3981694.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981694/SRX3981694.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981694/SRX3981694.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981694/SRX3981694.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:29:11: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:29:11: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:29:17: 1000000 INFO @ Mon, 12 Aug 2019 22:29:18: 1000000 INFO @ Mon, 12 Aug 2019 22:29:19: 1000000 INFO @ Mon, 12 Aug 2019 22:29:24: 2000000 INFO @ Mon, 12 Aug 2019 22:29:25: 2000000 INFO @ Mon, 12 Aug 2019 22:29:26: 2000000 INFO @ Mon, 12 Aug 2019 22:29:32: 3000000 INFO @ Mon, 12 Aug 2019 22:29:33: 3000000 INFO @ Mon, 12 Aug 2019 22:29:34: 3000000 INFO @ Mon, 12 Aug 2019 22:29:39: 4000000 INFO @ Mon, 12 Aug 2019 22:29:40: 4000000 INFO @ Mon, 12 Aug 2019 22:29:41: 4000000 INFO @ Mon, 12 Aug 2019 22:29:46: 5000000 INFO @ Mon, 12 Aug 2019 22:29:47: 5000000 INFO @ Mon, 12 Aug 2019 22:29:48: 5000000 INFO @ Mon, 12 Aug 2019 22:29:53: 6000000 INFO @ Mon, 12 Aug 2019 22:29:55: 6000000 INFO @ Mon, 12 Aug 2019 22:29:55: 6000000 INFO @ Mon, 12 Aug 2019 22:30:01: 7000000 INFO @ Mon, 12 Aug 2019 22:30:02: 7000000 INFO @ Mon, 12 Aug 2019 22:30:03: 7000000 INFO @ Mon, 12 Aug 2019 22:30:08: 8000000 INFO @ Mon, 12 Aug 2019 22:30:09: 8000000 INFO @ Mon, 12 Aug 2019 22:30:10: 8000000 INFO @ Mon, 12 Aug 2019 22:30:15: 9000000 INFO @ Mon, 12 Aug 2019 22:30:16: 9000000 INFO @ Mon, 12 Aug 2019 22:30:17: 9000000 INFO @ Mon, 12 Aug 2019 22:30:22: 10000000 INFO @ Mon, 12 Aug 2019 22:30:24: 10000000 INFO @ Mon, 12 Aug 2019 22:30:24: 10000000 INFO @ Mon, 12 Aug 2019 22:30:30: 11000000 INFO @ Mon, 12 Aug 2019 22:30:31: 11000000 INFO @ Mon, 12 Aug 2019 22:30:32: 11000000 INFO @ Mon, 12 Aug 2019 22:30:37: 12000000 INFO @ Mon, 12 Aug 2019 22:30:38: 12000000 INFO @ Mon, 12 Aug 2019 22:30:39: 12000000 INFO @ Mon, 12 Aug 2019 22:30:44: 13000000 INFO @ Mon, 12 Aug 2019 22:30:46: 13000000 INFO @ Mon, 12 Aug 2019 22:30:46: 13000000 INFO @ Mon, 12 Aug 2019 22:30:51: 14000000 INFO @ Mon, 12 Aug 2019 22:30:53: 14000000 INFO @ Mon, 12 Aug 2019 22:30:54: 14000000 INFO @ Mon, 12 Aug 2019 22:30:58: 15000000 INFO @ Mon, 12 Aug 2019 22:31:00: 15000000 INFO @ Mon, 12 Aug 2019 22:31:01: 15000000 INFO @ Mon, 12 Aug 2019 22:31:06: 16000000 INFO @ Mon, 12 Aug 2019 22:31:07: 16000000 INFO @ Mon, 12 Aug 2019 22:31:08: 16000000 INFO @ Mon, 12 Aug 2019 22:31:13: 17000000 INFO @ Mon, 12 Aug 2019 22:31:14: 17000000 INFO @ Mon, 12 Aug 2019 22:31:15: 17000000 INFO @ Mon, 12 Aug 2019 22:31:20: 18000000 INFO @ Mon, 12 Aug 2019 22:31:22: 18000000 INFO @ Mon, 12 Aug 2019 22:31:22: 18000000 INFO @ Mon, 12 Aug 2019 22:31:27: 19000000 INFO @ Mon, 12 Aug 2019 22:31:29: 19000000 INFO @ Mon, 12 Aug 2019 22:31:30: 19000000 INFO @ Mon, 12 Aug 2019 22:31:34: 20000000 INFO @ Mon, 12 Aug 2019 22:31:36: 20000000 INFO @ Mon, 12 Aug 2019 22:31:37: 20000000 INFO @ Mon, 12 Aug 2019 22:31:42: 21000000 INFO @ Mon, 12 Aug 2019 22:31:43: 21000000 INFO @ Mon, 12 Aug 2019 22:31:44: 21000000 INFO @ Mon, 12 Aug 2019 22:31:49: 22000000 INFO @ Mon, 12 Aug 2019 22:31:50: 22000000 INFO @ Mon, 12 Aug 2019 22:31:51: 22000000 INFO @ Mon, 12 Aug 2019 22:31:56: 23000000 INFO @ Mon, 12 Aug 2019 22:31:58: 23000000 INFO @ Mon, 12 Aug 2019 22:31:58: 23000000 INFO @ Mon, 12 Aug 2019 22:32:03: 24000000 INFO @ Mon, 12 Aug 2019 22:32:05: 24000000 INFO @ Mon, 12 Aug 2019 22:32:05: 24000000 INFO @ Mon, 12 Aug 2019 22:32:10: 25000000 INFO @ Mon, 12 Aug 2019 22:32:12: 25000000 INFO @ Mon, 12 Aug 2019 22:32:13: 25000000 INFO @ Mon, 12 Aug 2019 22:32:18: 26000000 INFO @ Mon, 12 Aug 2019 22:32:19: 26000000 INFO @ Mon, 12 Aug 2019 22:32:20: 26000000 INFO @ Mon, 12 Aug 2019 22:32:25: 27000000 INFO @ Mon, 12 Aug 2019 22:32:26: 27000000 INFO @ Mon, 12 Aug 2019 22:32:27: 27000000 INFO @ Mon, 12 Aug 2019 22:32:32: 28000000 INFO @ Mon, 12 Aug 2019 22:32:33: 28000000 INFO @ Mon, 12 Aug 2019 22:32:34: 28000000 INFO @ Mon, 12 Aug 2019 22:32:39: 29000000 INFO @ Mon, 12 Aug 2019 22:32:40: 29000000 INFO @ Mon, 12 Aug 2019 22:32:41: 29000000 INFO @ Mon, 12 Aug 2019 22:32:46: 30000000 INFO @ Mon, 12 Aug 2019 22:32:48: 30000000 INFO @ Mon, 12 Aug 2019 22:32:48: 30000000 INFO @ Mon, 12 Aug 2019 22:32:53: 31000000 INFO @ Mon, 12 Aug 2019 22:32:55: 31000000 INFO @ Mon, 12 Aug 2019 22:32:56: 31000000 INFO @ Mon, 12 Aug 2019 22:33:01: 32000000 INFO @ Mon, 12 Aug 2019 22:33:02: 32000000 INFO @ Mon, 12 Aug 2019 22:33:03: 32000000 INFO @ Mon, 12 Aug 2019 22:33:08: 33000000 INFO @ Mon, 12 Aug 2019 22:33:09: 33000000 INFO @ Mon, 12 Aug 2019 22:33:10: 33000000 INFO @ Mon, 12 Aug 2019 22:33:15: 34000000 INFO @ Mon, 12 Aug 2019 22:33:16: 34000000 INFO @ Mon, 12 Aug 2019 22:33:17: 34000000 INFO @ Mon, 12 Aug 2019 22:33:22: 35000000 INFO @ Mon, 12 Aug 2019 22:33:24: 35000000 INFO @ Mon, 12 Aug 2019 22:33:25: 35000000 INFO @ Mon, 12 Aug 2019 22:33:30: 36000000 INFO @ Mon, 12 Aug 2019 22:33:31: 36000000 INFO @ Mon, 12 Aug 2019 22:33:32: 36000000 INFO @ Mon, 12 Aug 2019 22:33:37: 37000000 INFO @ Mon, 12 Aug 2019 22:33:38: 37000000 INFO @ Mon, 12 Aug 2019 22:33:39: 37000000 INFO @ Mon, 12 Aug 2019 22:33:44: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:33:44: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:33:44: #1 total tags in treatment: 37942513 INFO @ Mon, 12 Aug 2019 22:33:44: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:33:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:33:45: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:33:45: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:33:45: #1 total tags in treatment: 37942513 INFO @ Mon, 12 Aug 2019 22:33:45: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:33:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:33:45: #1 tags after filtering in treatment: 37942513 INFO @ Mon, 12 Aug 2019 22:33:45: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:33:45: #1 finished! INFO @ Mon, 12 Aug 2019 22:33:45: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:33:45: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:33:46: #1 tags after filtering in treatment: 37942513 INFO @ Mon, 12 Aug 2019 22:33:46: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:33:46: #1 finished! INFO @ Mon, 12 Aug 2019 22:33:46: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:33:46: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:33:46: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:33:46: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:33:46: #1 total tags in treatment: 37942513 INFO @ Mon, 12 Aug 2019 22:33:46: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:33:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:33:47: #1 tags after filtering in treatment: 37942513 INFO @ Mon, 12 Aug 2019 22:33:47: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:33:47: #1 finished! INFO @ Mon, 12 Aug 2019 22:33:47: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:33:47: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:33:48: #2 number of paired peaks: 9 WARNING @ Mon, 12 Aug 2019 22:33:48: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:33:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981694/SRX3981694.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981694/SRX3981694.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981694/SRX3981694.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981694/SRX3981694.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 22:33:49: #2 number of paired peaks: 9 WARNING @ Mon, 12 Aug 2019 22:33:49: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:33:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981694/SRX3981694.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981694/SRX3981694.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981694/SRX3981694.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981694/SRX3981694.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 22:33:50: #2 number of paired peaks: 9 WARNING @ Mon, 12 Aug 2019 22:33:50: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:33:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981694/SRX3981694.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981694/SRX3981694.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981694/SRX3981694.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981694/SRX3981694.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。