Job ID = 2590460 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 9,654,496 reads read : 19,308,992 reads written : 9,654,496 reads 0-length : 9,654,496 spots read : 9,643,390 reads read : 19,286,780 reads written : 9,643,390 reads 0-length : 9,643,390 spots read : 9,679,966 reads read : 19,359,932 reads written : 9,679,966 reads 0-length : 9,679,966 spots read : 9,721,577 reads read : 19,443,154 reads written : 9,721,577 reads 0-length : 9,721,577 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:42 38699429 reads; of these: 38699429 (100.00%) were unpaired; of these: 1222924 (3.16%) aligned 0 times 27151097 (70.16%) aligned exactly 1 time 10325408 (26.68%) aligned >1 times 96.84% overall alignment rate Time searching: 00:16:42 Overall time: 00:16:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 5042199 / 37476505 = 0.1345 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 22:22:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981691/SRX3981691.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981691/SRX3981691.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981691/SRX3981691.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981691/SRX3981691.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:22:15: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:22:15: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:22:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981691/SRX3981691.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981691/SRX3981691.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981691/SRX3981691.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981691/SRX3981691.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:22:16: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:22:16: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:22:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981691/SRX3981691.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981691/SRX3981691.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981691/SRX3981691.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981691/SRX3981691.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:22:17: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:22:17: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:22:22: 1000000 INFO @ Mon, 12 Aug 2019 22:22:23: 1000000 INFO @ Mon, 12 Aug 2019 22:22:24: 1000000 INFO @ Mon, 12 Aug 2019 22:22:29: 2000000 INFO @ Mon, 12 Aug 2019 22:22:30: 2000000 INFO @ Mon, 12 Aug 2019 22:22:31: 2000000 INFO @ Mon, 12 Aug 2019 22:22:35: 3000000 INFO @ Mon, 12 Aug 2019 22:22:37: 3000000 INFO @ Mon, 12 Aug 2019 22:22:37: 3000000 INFO @ Mon, 12 Aug 2019 22:22:42: 4000000 INFO @ Mon, 12 Aug 2019 22:22:43: 4000000 INFO @ Mon, 12 Aug 2019 22:22:44: 4000000 INFO @ Mon, 12 Aug 2019 22:22:49: 5000000 INFO @ Mon, 12 Aug 2019 22:22:50: 5000000 INFO @ Mon, 12 Aug 2019 22:22:51: 5000000 INFO @ Mon, 12 Aug 2019 22:22:55: 6000000 INFO @ Mon, 12 Aug 2019 22:22:57: 6000000 INFO @ Mon, 12 Aug 2019 22:22:58: 6000000 INFO @ Mon, 12 Aug 2019 22:23:02: 7000000 INFO @ Mon, 12 Aug 2019 22:23:04: 7000000 INFO @ Mon, 12 Aug 2019 22:23:04: 7000000 INFO @ Mon, 12 Aug 2019 22:23:09: 8000000 INFO @ Mon, 12 Aug 2019 22:23:10: 8000000 INFO @ Mon, 12 Aug 2019 22:23:11: 8000000 INFO @ Mon, 12 Aug 2019 22:23:15: 9000000 INFO @ Mon, 12 Aug 2019 22:23:17: 9000000 INFO @ Mon, 12 Aug 2019 22:23:18: 9000000 INFO @ Mon, 12 Aug 2019 22:23:22: 10000000 INFO @ Mon, 12 Aug 2019 22:23:24: 10000000 INFO @ Mon, 12 Aug 2019 22:23:25: 10000000 INFO @ Mon, 12 Aug 2019 22:23:29: 11000000 INFO @ Mon, 12 Aug 2019 22:23:31: 11000000 INFO @ Mon, 12 Aug 2019 22:23:31: 11000000 INFO @ Mon, 12 Aug 2019 22:23:35: 12000000 INFO @ Mon, 12 Aug 2019 22:23:38: 12000000 INFO @ Mon, 12 Aug 2019 22:23:38: 12000000 INFO @ Mon, 12 Aug 2019 22:23:42: 13000000 INFO @ Mon, 12 Aug 2019 22:23:44: 13000000 INFO @ Mon, 12 Aug 2019 22:23:45: 13000000 INFO @ Mon, 12 Aug 2019 22:23:50: 14000000 INFO @ Mon, 12 Aug 2019 22:23:51: 14000000 INFO @ Mon, 12 Aug 2019 22:23:52: 14000000 INFO @ Mon, 12 Aug 2019 22:23:57: 15000000 INFO @ Mon, 12 Aug 2019 22:23:58: 15000000 INFO @ Mon, 12 Aug 2019 22:23:59: 15000000 INFO @ Mon, 12 Aug 2019 22:24:04: 16000000 INFO @ Mon, 12 Aug 2019 22:24:05: 16000000 INFO @ Mon, 12 Aug 2019 22:24:05: 16000000 INFO @ Mon, 12 Aug 2019 22:24:11: 17000000 INFO @ Mon, 12 Aug 2019 22:24:12: 17000000 INFO @ Mon, 12 Aug 2019 22:24:12: 17000000 INFO @ Mon, 12 Aug 2019 22:24:17: 18000000 INFO @ Mon, 12 Aug 2019 22:24:19: 18000000 INFO @ Mon, 12 Aug 2019 22:24:19: 18000000 INFO @ Mon, 12 Aug 2019 22:24:24: 19000000 INFO @ Mon, 12 Aug 2019 22:24:26: 19000000 INFO @ Mon, 12 Aug 2019 22:24:26: 19000000 INFO @ Mon, 12 Aug 2019 22:24:31: 20000000 INFO @ Mon, 12 Aug 2019 22:24:33: 20000000 INFO @ Mon, 12 Aug 2019 22:24:33: 20000000 INFO @ Mon, 12 Aug 2019 22:24:37: 21000000 INFO @ Mon, 12 Aug 2019 22:24:40: 21000000 INFO @ Mon, 12 Aug 2019 22:24:40: 21000000 INFO @ Mon, 12 Aug 2019 22:24:44: 22000000 INFO @ Mon, 12 Aug 2019 22:24:47: 22000000 INFO @ Mon, 12 Aug 2019 22:24:47: 22000000 INFO @ Mon, 12 Aug 2019 22:24:51: 23000000 INFO @ Mon, 12 Aug 2019 22:24:54: 23000000 INFO @ Mon, 12 Aug 2019 22:24:54: 23000000 INFO @ Mon, 12 Aug 2019 22:24:58: 24000000 INFO @ Mon, 12 Aug 2019 22:25:01: 24000000 INFO @ Mon, 12 Aug 2019 22:25:01: 24000000 INFO @ Mon, 12 Aug 2019 22:25:04: 25000000 INFO @ Mon, 12 Aug 2019 22:25:08: 25000000 INFO @ Mon, 12 Aug 2019 22:25:08: 25000000 INFO @ Mon, 12 Aug 2019 22:25:11: 26000000 INFO @ Mon, 12 Aug 2019 22:25:15: 26000000 INFO @ Mon, 12 Aug 2019 22:25:15: 26000000 INFO @ Mon, 12 Aug 2019 22:25:18: 27000000 INFO @ Mon, 12 Aug 2019 22:25:22: 27000000 INFO @ Mon, 12 Aug 2019 22:25:22: 27000000 INFO @ Mon, 12 Aug 2019 22:25:25: 28000000 INFO @ Mon, 12 Aug 2019 22:25:29: 28000000 INFO @ Mon, 12 Aug 2019 22:25:29: 28000000 INFO @ Mon, 12 Aug 2019 22:25:31: 29000000 INFO @ Mon, 12 Aug 2019 22:25:36: 29000000 INFO @ Mon, 12 Aug 2019 22:25:36: 29000000 INFO @ Mon, 12 Aug 2019 22:25:38: 30000000 INFO @ Mon, 12 Aug 2019 22:25:42: 30000000 INFO @ Mon, 12 Aug 2019 22:25:43: 30000000 INFO @ Mon, 12 Aug 2019 22:25:45: 31000000 INFO @ Mon, 12 Aug 2019 22:25:49: 31000000 INFO @ Mon, 12 Aug 2019 22:25:50: 31000000 INFO @ Mon, 12 Aug 2019 22:25:51: 32000000 INFO @ Mon, 12 Aug 2019 22:25:55: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:25:55: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:25:55: #1 total tags in treatment: 32434306 INFO @ Mon, 12 Aug 2019 22:25:55: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:25:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:25:55: #1 tags after filtering in treatment: 32434306 INFO @ Mon, 12 Aug 2019 22:25:55: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:25:55: #1 finished! INFO @ Mon, 12 Aug 2019 22:25:55: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:25:55: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:25:56: 32000000 INFO @ Mon, 12 Aug 2019 22:25:56: 32000000 INFO @ Mon, 12 Aug 2019 22:25:58: #2 number of paired peaks: 28 WARNING @ Mon, 12 Aug 2019 22:25:58: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:25:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981691/SRX3981691.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981691/SRX3981691.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981691/SRX3981691.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981691/SRX3981691.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 22:25:59: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:25:59: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:25:59: #1 total tags in treatment: 32434306 INFO @ Mon, 12 Aug 2019 22:25:59: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:25:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:26:00: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:26:00: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:26:00: #1 total tags in treatment: 32434306 INFO @ Mon, 12 Aug 2019 22:26:00: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:26:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:26:00: #1 tags after filtering in treatment: 32434306 INFO @ Mon, 12 Aug 2019 22:26:00: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:26:00: #1 finished! INFO @ Mon, 12 Aug 2019 22:26:00: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:26:00: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:26:00: #1 tags after filtering in treatment: 32434306 INFO @ Mon, 12 Aug 2019 22:26:00: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:26:00: #1 finished! INFO @ Mon, 12 Aug 2019 22:26:00: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:26:00: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:26:03: #2 number of paired peaks: 28 WARNING @ Mon, 12 Aug 2019 22:26:03: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:26:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981691/SRX3981691.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981691/SRX3981691.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981691/SRX3981691.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981691/SRX3981691.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 22:26:03: #2 number of paired peaks: 28 WARNING @ Mon, 12 Aug 2019 22:26:03: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:26:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981691/SRX3981691.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981691/SRX3981691.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981691/SRX3981691.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981691/SRX3981691.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。