Job ID = 6528056 SRX = SRX3981676 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:11:50 prefetch.2.10.7: 1) Downloading 'SRR7050412'... 2020-06-29T14:11:50 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:13:01 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:13:01 prefetch.2.10.7: 'SRR7050412' is valid 2020-06-29T14:13:01 prefetch.2.10.7: 1) 'SRR7050412' was downloaded successfully 2020-06-29T14:13:01 prefetch.2.10.7: 'SRR7050412' has 0 unresolved dependencies Read 14999062 spots for SRR7050412/SRR7050412.sra Written 14999062 spots for SRR7050412/SRR7050412.sra 2020-06-29T14:13:59 prefetch.2.10.7: 1) Downloading 'SRR7050413'... 2020-06-29T14:13:59 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:15:21 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:15:21 prefetch.2.10.7: 'SRR7050413' is valid 2020-06-29T14:15:21 prefetch.2.10.7: 1) 'SRR7050413' was downloaded successfully 2020-06-29T14:15:21 prefetch.2.10.7: 'SRR7050413' has 0 unresolved dependencies Read 15008005 spots for SRR7050413/SRR7050413.sra Written 15008005 spots for SRR7050413/SRR7050413.sra 2020-06-29T14:16:21 prefetch.2.10.7: 1) Downloading 'SRR7050414'... 2020-06-29T14:16:21 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:17:28 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:17:29 prefetch.2.10.7: 'SRR7050414' is valid 2020-06-29T14:17:29 prefetch.2.10.7: 1) 'SRR7050414' was downloaded successfully 2020-06-29T14:17:29 prefetch.2.10.7: 'SRR7050414' has 0 unresolved dependencies Read 14920844 spots for SRR7050414/SRR7050414.sra Written 14920844 spots for SRR7050414/SRR7050414.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:00 44927911 reads; of these: 44927911 (100.00%) were unpaired; of these: 1298530 (2.89%) aligned 0 times 32162653 (71.59%) aligned exactly 1 time 11466728 (25.52%) aligned >1 times 97.11% overall alignment rate Time searching: 00:14:00 Overall time: 00:14:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 5251384 / 43629381 = 0.1204 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:51:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981676/SRX3981676.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981676/SRX3981676.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981676/SRX3981676.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981676/SRX3981676.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:51:06: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:51:06: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:51:12: 1000000 INFO @ Mon, 29 Jun 2020 23:51:17: 2000000 INFO @ Mon, 29 Jun 2020 23:51:23: 3000000 INFO @ Mon, 29 Jun 2020 23:51:28: 4000000 INFO @ Mon, 29 Jun 2020 23:51:33: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:51:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981676/SRX3981676.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981676/SRX3981676.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981676/SRX3981676.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981676/SRX3981676.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:51:36: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:51:36: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:51:39: 6000000 INFO @ Mon, 29 Jun 2020 23:51:42: 1000000 INFO @ Mon, 29 Jun 2020 23:51:45: 7000000 INFO @ Mon, 29 Jun 2020 23:51:48: 2000000 INFO @ Mon, 29 Jun 2020 23:51:51: 8000000 INFO @ Mon, 29 Jun 2020 23:51:54: 3000000 INFO @ Mon, 29 Jun 2020 23:51:57: 9000000 INFO @ Mon, 29 Jun 2020 23:51:59: 4000000 INFO @ Mon, 29 Jun 2020 23:52:02: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:52:05: 5000000 INFO @ Mon, 29 Jun 2020 23:52:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981676/SRX3981676.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981676/SRX3981676.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981676/SRX3981676.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981676/SRX3981676.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:52:06: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:52:06: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:52:08: 11000000 INFO @ Mon, 29 Jun 2020 23:52:11: 6000000 INFO @ Mon, 29 Jun 2020 23:52:12: 1000000 INFO @ Mon, 29 Jun 2020 23:52:14: 12000000 INFO @ Mon, 29 Jun 2020 23:52:17: 7000000 INFO @ Mon, 29 Jun 2020 23:52:18: 2000000 INFO @ Mon, 29 Jun 2020 23:52:20: 13000000 INFO @ Mon, 29 Jun 2020 23:52:22: 8000000 INFO @ Mon, 29 Jun 2020 23:52:24: 3000000 INFO @ Mon, 29 Jun 2020 23:52:26: 14000000 INFO @ Mon, 29 Jun 2020 23:52:28: 9000000 INFO @ Mon, 29 Jun 2020 23:52:30: 4000000 INFO @ Mon, 29 Jun 2020 23:52:32: 15000000 INFO @ Mon, 29 Jun 2020 23:52:34: 10000000 INFO @ Mon, 29 Jun 2020 23:52:36: 5000000 INFO @ Mon, 29 Jun 2020 23:52:38: 16000000 INFO @ Mon, 29 Jun 2020 23:52:40: 11000000 INFO @ Mon, 29 Jun 2020 23:52:41: 6000000 INFO @ Mon, 29 Jun 2020 23:52:44: 17000000 INFO @ Mon, 29 Jun 2020 23:52:45: 12000000 INFO @ Mon, 29 Jun 2020 23:52:47: 7000000 INFO @ Mon, 29 Jun 2020 23:52:49: 18000000 INFO @ Mon, 29 Jun 2020 23:52:51: 13000000 INFO @ Mon, 29 Jun 2020 23:52:53: 8000000 INFO @ Mon, 29 Jun 2020 23:52:55: 19000000 INFO @ Mon, 29 Jun 2020 23:52:57: 14000000 INFO @ Mon, 29 Jun 2020 23:52:59: 9000000 INFO @ Mon, 29 Jun 2020 23:53:01: 20000000 INFO @ Mon, 29 Jun 2020 23:53:03: 15000000 INFO @ Mon, 29 Jun 2020 23:53:05: 10000000 INFO @ Mon, 29 Jun 2020 23:53:07: 21000000 INFO @ Mon, 29 Jun 2020 23:53:08: 16000000 INFO @ Mon, 29 Jun 2020 23:53:10: 11000000 INFO @ Mon, 29 Jun 2020 23:53:13: 22000000 INFO @ Mon, 29 Jun 2020 23:53:14: 17000000 INFO @ Mon, 29 Jun 2020 23:53:16: 12000000 INFO @ Mon, 29 Jun 2020 23:53:19: 23000000 INFO @ Mon, 29 Jun 2020 23:53:20: 18000000 INFO @ Mon, 29 Jun 2020 23:53:22: 13000000 INFO @ Mon, 29 Jun 2020 23:53:24: 24000000 INFO @ Mon, 29 Jun 2020 23:53:26: 19000000 INFO @ Mon, 29 Jun 2020 23:53:28: 14000000 INFO @ Mon, 29 Jun 2020 23:53:30: 25000000 INFO @ Mon, 29 Jun 2020 23:53:31: 20000000 INFO @ Mon, 29 Jun 2020 23:53:33: 15000000 INFO @ Mon, 29 Jun 2020 23:53:36: 26000000 INFO @ Mon, 29 Jun 2020 23:53:37: 21000000 INFO @ Mon, 29 Jun 2020 23:53:39: 16000000 INFO @ Mon, 29 Jun 2020 23:53:42: 27000000 INFO @ Mon, 29 Jun 2020 23:53:43: 22000000 INFO @ Mon, 29 Jun 2020 23:53:45: 17000000 INFO @ Mon, 29 Jun 2020 23:53:48: 28000000 INFO @ Mon, 29 Jun 2020 23:53:48: 23000000 INFO @ Mon, 29 Jun 2020 23:53:51: 18000000 INFO @ Mon, 29 Jun 2020 23:53:53: 29000000 INFO @ Mon, 29 Jun 2020 23:53:54: 24000000 INFO @ Mon, 29 Jun 2020 23:53:56: 19000000 INFO @ Mon, 29 Jun 2020 23:53:59: 30000000 INFO @ Mon, 29 Jun 2020 23:54:00: 25000000 INFO @ Mon, 29 Jun 2020 23:54:03: 20000000 INFO @ Mon, 29 Jun 2020 23:54:06: 31000000 INFO @ Mon, 29 Jun 2020 23:54:06: 26000000 INFO @ Mon, 29 Jun 2020 23:54:09: 21000000 INFO @ Mon, 29 Jun 2020 23:54:12: 32000000 INFO @ Mon, 29 Jun 2020 23:54:12: 27000000 INFO @ Mon, 29 Jun 2020 23:54:15: 22000000 INFO @ Mon, 29 Jun 2020 23:54:18: 33000000 INFO @ Mon, 29 Jun 2020 23:54:18: 28000000 INFO @ Mon, 29 Jun 2020 23:54:21: 23000000 INFO @ Mon, 29 Jun 2020 23:54:24: 34000000 INFO @ Mon, 29 Jun 2020 23:54:24: 29000000 INFO @ Mon, 29 Jun 2020 23:54:27: 24000000 INFO @ Mon, 29 Jun 2020 23:54:30: 35000000 INFO @ Mon, 29 Jun 2020 23:54:30: 30000000 INFO @ Mon, 29 Jun 2020 23:54:33: 25000000 INFO @ Mon, 29 Jun 2020 23:54:36: 36000000 INFO @ Mon, 29 Jun 2020 23:54:36: 31000000 INFO @ Mon, 29 Jun 2020 23:54:38: 26000000 INFO @ Mon, 29 Jun 2020 23:54:42: 37000000 INFO @ Mon, 29 Jun 2020 23:54:42: 32000000 INFO @ Mon, 29 Jun 2020 23:54:44: 27000000 INFO @ Mon, 29 Jun 2020 23:54:48: 38000000 INFO @ Mon, 29 Jun 2020 23:54:48: 33000000 INFO @ Mon, 29 Jun 2020 23:54:50: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:54:50: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:54:50: #1 total tags in treatment: 38377997 INFO @ Mon, 29 Jun 2020 23:54:50: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:54:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:54:50: 28000000 INFO @ Mon, 29 Jun 2020 23:54:51: #1 tags after filtering in treatment: 38377997 INFO @ Mon, 29 Jun 2020 23:54:51: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:54:51: #1 finished! INFO @ Mon, 29 Jun 2020 23:54:51: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:54:51: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:54:53: #2 number of paired peaks: 4 WARNING @ Mon, 29 Jun 2020 23:54:53: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:54:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981676/SRX3981676.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981676/SRX3981676.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981676/SRX3981676.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981676/SRX3981676.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:54:54: 34000000 INFO @ Mon, 29 Jun 2020 23:54:56: 29000000 INFO @ Mon, 29 Jun 2020 23:54:59: 35000000 INFO @ Mon, 29 Jun 2020 23:55:02: 30000000 INFO @ Mon, 29 Jun 2020 23:55:05: 36000000 INFO @ Mon, 29 Jun 2020 23:55:08: 31000000 INFO @ Mon, 29 Jun 2020 23:55:11: 37000000 INFO @ Mon, 29 Jun 2020 23:55:13: 32000000 INFO @ Mon, 29 Jun 2020 23:55:17: 38000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:55:19: 33000000 INFO @ Mon, 29 Jun 2020 23:55:19: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:55:19: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:55:19: #1 total tags in treatment: 38377997 INFO @ Mon, 29 Jun 2020 23:55:19: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:55:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:55:20: #1 tags after filtering in treatment: 38377997 INFO @ Mon, 29 Jun 2020 23:55:20: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:55:20: #1 finished! INFO @ Mon, 29 Jun 2020 23:55:20: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:55:20: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:55:22: #2 number of paired peaks: 4 WARNING @ Mon, 29 Jun 2020 23:55:22: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:55:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981676/SRX3981676.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981676/SRX3981676.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981676/SRX3981676.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981676/SRX3981676.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:55:25: 34000000 INFO @ Mon, 29 Jun 2020 23:55:30: 35000000 INFO @ Mon, 29 Jun 2020 23:55:36: 36000000 INFO @ Mon, 29 Jun 2020 23:55:41: 37000000 INFO @ Mon, 29 Jun 2020 23:55:47: 38000000 INFO @ Mon, 29 Jun 2020 23:55:49: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:55:49: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:55:49: #1 total tags in treatment: 38377997 INFO @ Mon, 29 Jun 2020 23:55:49: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:55:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:55:50: #1 tags after filtering in treatment: 38377997 INFO @ Mon, 29 Jun 2020 23:55:50: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:55:50: #1 finished! INFO @ Mon, 29 Jun 2020 23:55:50: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:55:50: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:55:52: #2 number of paired peaks: 4 WARNING @ Mon, 29 Jun 2020 23:55:52: Too few paired peaks (4) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:55:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981676/SRX3981676.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981676/SRX3981676.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981676/SRX3981676.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981676/SRX3981676.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。