Job ID = 6528053 SRX = SRX3981672 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:03:06 prefetch.2.10.7: 1) Downloading 'SRR7050400'... 2020-06-29T14:03:06 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:04:27 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:04:27 prefetch.2.10.7: 'SRR7050400' is valid 2020-06-29T14:04:27 prefetch.2.10.7: 1) 'SRR7050400' was downloaded successfully 2020-06-29T14:04:27 prefetch.2.10.7: 'SRR7050400' has 0 unresolved dependencies Read 12434565 spots for SRR7050400/SRR7050400.sra Written 12434565 spots for SRR7050400/SRR7050400.sra 2020-06-29T14:05:20 prefetch.2.10.7: 1) Downloading 'SRR7050401'... 2020-06-29T14:05:20 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:06:18 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:06:18 prefetch.2.10.7: 'SRR7050401' is valid 2020-06-29T14:06:18 prefetch.2.10.7: 1) 'SRR7050401' was downloaded successfully 2020-06-29T14:06:18 prefetch.2.10.7: 'SRR7050401' has 0 unresolved dependencies Read 12434688 spots for SRR7050401/SRR7050401.sra Written 12434688 spots for SRR7050401/SRR7050401.sra 2020-06-29T14:07:11 prefetch.2.10.7: 1) Downloading 'SRR7050402'... 2020-06-29T14:07:11 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:08:51 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:08:52 prefetch.2.10.7: 'SRR7050402' is valid 2020-06-29T14:08:52 prefetch.2.10.7: 1) 'SRR7050402' was downloaded successfully 2020-06-29T14:08:52 prefetch.2.10.7: 'SRR7050402' has 0 unresolved dependencies Read 12354744 spots for SRR7050402/SRR7050402.sra Written 12354744 spots for SRR7050402/SRR7050402.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:53 37223997 reads; of these: 37223997 (100.00%) were unpaired; of these: 1097836 (2.95%) aligned 0 times 26722754 (71.79%) aligned exactly 1 time 9403407 (25.26%) aligned >1 times 97.05% overall alignment rate Time searching: 00:11:53 Overall time: 00:11:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 3783806 / 36126161 = 0.1047 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:38:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981672/SRX3981672.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981672/SRX3981672.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981672/SRX3981672.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981672/SRX3981672.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:38:10: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:38:10: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:38:16: 1000000 INFO @ Mon, 29 Jun 2020 23:38:22: 2000000 INFO @ Mon, 29 Jun 2020 23:38:28: 3000000 INFO @ Mon, 29 Jun 2020 23:38:34: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:38:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981672/SRX3981672.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981672/SRX3981672.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981672/SRX3981672.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981672/SRX3981672.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:38:40: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:38:40: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:38:40: 5000000 INFO @ Mon, 29 Jun 2020 23:38:46: 1000000 INFO @ Mon, 29 Jun 2020 23:38:46: 6000000 INFO @ Mon, 29 Jun 2020 23:38:53: 7000000 INFO @ Mon, 29 Jun 2020 23:38:53: 2000000 INFO @ Mon, 29 Jun 2020 23:38:59: 8000000 INFO @ Mon, 29 Jun 2020 23:38:59: 3000000 INFO @ Mon, 29 Jun 2020 23:39:06: 9000000 INFO @ Mon, 29 Jun 2020 23:39:06: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:39:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981672/SRX3981672.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981672/SRX3981672.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981672/SRX3981672.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981672/SRX3981672.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:39:10: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:39:10: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:39:12: 10000000 INFO @ Mon, 29 Jun 2020 23:39:12: 5000000 INFO @ Mon, 29 Jun 2020 23:39:16: 1000000 INFO @ Mon, 29 Jun 2020 23:39:19: 11000000 INFO @ Mon, 29 Jun 2020 23:39:19: 6000000 INFO @ Mon, 29 Jun 2020 23:39:23: 2000000 INFO @ Mon, 29 Jun 2020 23:39:25: 12000000 INFO @ Mon, 29 Jun 2020 23:39:25: 7000000 INFO @ Mon, 29 Jun 2020 23:39:29: 3000000 INFO @ Mon, 29 Jun 2020 23:39:32: 13000000 INFO @ Mon, 29 Jun 2020 23:39:32: 8000000 INFO @ Mon, 29 Jun 2020 23:39:36: 4000000 INFO @ Mon, 29 Jun 2020 23:39:38: 14000000 INFO @ Mon, 29 Jun 2020 23:39:38: 9000000 INFO @ Mon, 29 Jun 2020 23:39:42: 5000000 INFO @ Mon, 29 Jun 2020 23:39:45: 15000000 INFO @ Mon, 29 Jun 2020 23:39:45: 10000000 INFO @ Mon, 29 Jun 2020 23:39:48: 6000000 INFO @ Mon, 29 Jun 2020 23:39:52: 16000000 INFO @ Mon, 29 Jun 2020 23:39:52: 11000000 INFO @ Mon, 29 Jun 2020 23:39:55: 7000000 INFO @ Mon, 29 Jun 2020 23:39:58: 17000000 INFO @ Mon, 29 Jun 2020 23:39:58: 12000000 INFO @ Mon, 29 Jun 2020 23:40:01: 8000000 INFO @ Mon, 29 Jun 2020 23:40:05: 18000000 INFO @ Mon, 29 Jun 2020 23:40:05: 13000000 INFO @ Mon, 29 Jun 2020 23:40:08: 9000000 INFO @ Mon, 29 Jun 2020 23:40:11: 19000000 INFO @ Mon, 29 Jun 2020 23:40:12: 14000000 INFO @ Mon, 29 Jun 2020 23:40:15: 10000000 INFO @ Mon, 29 Jun 2020 23:40:18: 20000000 INFO @ Mon, 29 Jun 2020 23:40:18: 15000000 INFO @ Mon, 29 Jun 2020 23:40:21: 11000000 INFO @ Mon, 29 Jun 2020 23:40:25: 21000000 INFO @ Mon, 29 Jun 2020 23:40:25: 16000000 INFO @ Mon, 29 Jun 2020 23:40:28: 12000000 INFO @ Mon, 29 Jun 2020 23:40:31: 22000000 INFO @ Mon, 29 Jun 2020 23:40:32: 17000000 INFO @ Mon, 29 Jun 2020 23:40:34: 13000000 INFO @ Mon, 29 Jun 2020 23:40:38: 23000000 INFO @ Mon, 29 Jun 2020 23:40:38: 18000000 INFO @ Mon, 29 Jun 2020 23:40:41: 14000000 INFO @ Mon, 29 Jun 2020 23:40:45: 19000000 INFO @ Mon, 29 Jun 2020 23:40:45: 24000000 INFO @ Mon, 29 Jun 2020 23:40:47: 15000000 INFO @ Mon, 29 Jun 2020 23:40:51: 20000000 INFO @ Mon, 29 Jun 2020 23:40:52: 25000000 INFO @ Mon, 29 Jun 2020 23:40:54: 16000000 INFO @ Mon, 29 Jun 2020 23:40:58: 21000000 INFO @ Mon, 29 Jun 2020 23:40:58: 26000000 INFO @ Mon, 29 Jun 2020 23:41:00: 17000000 INFO @ Mon, 29 Jun 2020 23:41:05: 22000000 INFO @ Mon, 29 Jun 2020 23:41:05: 27000000 INFO @ Mon, 29 Jun 2020 23:41:07: 18000000 INFO @ Mon, 29 Jun 2020 23:41:11: 23000000 INFO @ Mon, 29 Jun 2020 23:41:12: 28000000 INFO @ Mon, 29 Jun 2020 23:41:13: 19000000 INFO @ Mon, 29 Jun 2020 23:41:18: 24000000 INFO @ Mon, 29 Jun 2020 23:41:19: 29000000 INFO @ Mon, 29 Jun 2020 23:41:20: 20000000 INFO @ Mon, 29 Jun 2020 23:41:25: 25000000 INFO @ Mon, 29 Jun 2020 23:41:25: 30000000 INFO @ Mon, 29 Jun 2020 23:41:26: 21000000 INFO @ Mon, 29 Jun 2020 23:41:31: 26000000 INFO @ Mon, 29 Jun 2020 23:41:32: 31000000 INFO @ Mon, 29 Jun 2020 23:41:33: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:41:38: 27000000 INFO @ Mon, 29 Jun 2020 23:41:39: 32000000 INFO @ Mon, 29 Jun 2020 23:41:39: 23000000 INFO @ Mon, 29 Jun 2020 23:41:41: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:41:41: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:41:41: #1 total tags in treatment: 32342355 INFO @ Mon, 29 Jun 2020 23:41:41: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:41:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:41:42: #1 tags after filtering in treatment: 32342355 INFO @ Mon, 29 Jun 2020 23:41:42: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:41:42: #1 finished! INFO @ Mon, 29 Jun 2020 23:41:42: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:41:42: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:41:44: #2 number of paired peaks: 25 WARNING @ Mon, 29 Jun 2020 23:41:44: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:41:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981672/SRX3981672.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981672/SRX3981672.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981672/SRX3981672.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981672/SRX3981672.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:41:45: 28000000 INFO @ Mon, 29 Jun 2020 23:41:46: 24000000 INFO @ Mon, 29 Jun 2020 23:41:51: 29000000 INFO @ Mon, 29 Jun 2020 23:41:52: 25000000 INFO @ Mon, 29 Jun 2020 23:41:58: 30000000 INFO @ Mon, 29 Jun 2020 23:41:59: 26000000 INFO @ Mon, 29 Jun 2020 23:42:04: 31000000 INFO @ Mon, 29 Jun 2020 23:42:05: 27000000 INFO @ Mon, 29 Jun 2020 23:42:11: 32000000 INFO @ Mon, 29 Jun 2020 23:42:12: 28000000 INFO @ Mon, 29 Jun 2020 23:42:13: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:42:13: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:42:13: #1 total tags in treatment: 32342355 INFO @ Mon, 29 Jun 2020 23:42:13: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:42:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:42:14: #1 tags after filtering in treatment: 32342355 INFO @ Mon, 29 Jun 2020 23:42:14: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:42:14: #1 finished! INFO @ Mon, 29 Jun 2020 23:42:14: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:42:14: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:42:16: #2 number of paired peaks: 25 WARNING @ Mon, 29 Jun 2020 23:42:16: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:42:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981672/SRX3981672.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981672/SRX3981672.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981672/SRX3981672.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981672/SRX3981672.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:42:18: 29000000 INFO @ Mon, 29 Jun 2020 23:42:24: 30000000 INFO @ Mon, 29 Jun 2020 23:42:31: 31000000 INFO @ Mon, 29 Jun 2020 23:42:37: 32000000 INFO @ Mon, 29 Jun 2020 23:42:39: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:42:39: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:42:39: #1 total tags in treatment: 32342355 INFO @ Mon, 29 Jun 2020 23:42:39: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:42:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:42:39: #1 tags after filtering in treatment: 32342355 INFO @ Mon, 29 Jun 2020 23:42:39: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:42:39: #1 finished! INFO @ Mon, 29 Jun 2020 23:42:39: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:42:39: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:42:42: #2 number of paired peaks: 25 WARNING @ Mon, 29 Jun 2020 23:42:42: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:42:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981672/SRX3981672.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981672/SRX3981672.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981672/SRX3981672.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981672/SRX3981672.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。