Job ID = 2590435 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 11,567,411 reads read : 23,134,822 reads written : 11,567,411 reads 0-length : 11,567,411 spots read : 11,568,697 reads read : 23,137,394 reads written : 11,568,697 reads 0-length : 11,568,697 spots read : 11,507,009 reads read : 23,014,018 reads written : 11,507,009 reads 0-length : 11,507,009 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:39 34643117 reads; of these: 34643117 (100.00%) were unpaired; of these: 1080211 (3.12%) aligned 0 times 24677513 (71.23%) aligned exactly 1 time 8885393 (25.65%) aligned >1 times 96.88% overall alignment rate Time searching: 00:12:39 Overall time: 00:12:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 3565952 / 33562906 = 0.1062 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 21:44:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981669/SRX3981669.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981669/SRX3981669.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981669/SRX3981669.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981669/SRX3981669.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 21:44:23: #1 read tag files... INFO @ Mon, 12 Aug 2019 21:44:23: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 21:44:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981669/SRX3981669.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981669/SRX3981669.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981669/SRX3981669.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981669/SRX3981669.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 21:44:24: #1 read tag files... INFO @ Mon, 12 Aug 2019 21:44:24: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 21:44:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981669/SRX3981669.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981669/SRX3981669.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981669/SRX3981669.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981669/SRX3981669.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 21:44:25: #1 read tag files... INFO @ Mon, 12 Aug 2019 21:44:25: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 21:44:32: 1000000 INFO @ Mon, 12 Aug 2019 21:44:34: 1000000 INFO @ Mon, 12 Aug 2019 21:44:35: 1000000 INFO @ Mon, 12 Aug 2019 21:44:40: 2000000 INFO @ Mon, 12 Aug 2019 21:44:42: 2000000 INFO @ Mon, 12 Aug 2019 21:44:44: 2000000 INFO @ Mon, 12 Aug 2019 21:44:49: 3000000 INFO @ Mon, 12 Aug 2019 21:44:52: 3000000 INFO @ Mon, 12 Aug 2019 21:44:54: 3000000 INFO @ Mon, 12 Aug 2019 21:44:58: 4000000 INFO @ Mon, 12 Aug 2019 21:45:01: 4000000 INFO @ Mon, 12 Aug 2019 21:45:04: 4000000 INFO @ Mon, 12 Aug 2019 21:45:07: 5000000 INFO @ Mon, 12 Aug 2019 21:45:10: 5000000 INFO @ Mon, 12 Aug 2019 21:45:14: 5000000 INFO @ Mon, 12 Aug 2019 21:45:15: 6000000 INFO @ Mon, 12 Aug 2019 21:45:19: 6000000 INFO @ Mon, 12 Aug 2019 21:45:23: 6000000 INFO @ Mon, 12 Aug 2019 21:45:23: 7000000 INFO @ Mon, 12 Aug 2019 21:45:28: 7000000 INFO @ Mon, 12 Aug 2019 21:45:32: 8000000 INFO @ Mon, 12 Aug 2019 21:45:33: 7000000 INFO @ Mon, 12 Aug 2019 21:45:36: 8000000 INFO @ Mon, 12 Aug 2019 21:45:40: 9000000 INFO @ Mon, 12 Aug 2019 21:45:42: 8000000 INFO @ Mon, 12 Aug 2019 21:45:45: 9000000 INFO @ Mon, 12 Aug 2019 21:45:48: 10000000 INFO @ Mon, 12 Aug 2019 21:45:51: 9000000 INFO @ Mon, 12 Aug 2019 21:45:54: 10000000 INFO @ Mon, 12 Aug 2019 21:45:56: 11000000 INFO @ Mon, 12 Aug 2019 21:46:00: 10000000 INFO @ Mon, 12 Aug 2019 21:46:02: 11000000 INFO @ Mon, 12 Aug 2019 21:46:04: 12000000 INFO @ Mon, 12 Aug 2019 21:46:09: 11000000 INFO @ Mon, 12 Aug 2019 21:46:11: 12000000 INFO @ Mon, 12 Aug 2019 21:46:12: 13000000 INFO @ Mon, 12 Aug 2019 21:46:18: 12000000 INFO @ Mon, 12 Aug 2019 21:46:19: 13000000 INFO @ Mon, 12 Aug 2019 21:46:20: 14000000 INFO @ Mon, 12 Aug 2019 21:46:27: 13000000 INFO @ Mon, 12 Aug 2019 21:46:27: 15000000 INFO @ Mon, 12 Aug 2019 21:46:28: 14000000 INFO @ Mon, 12 Aug 2019 21:46:34: 16000000 INFO @ Mon, 12 Aug 2019 21:46:36: 14000000 INFO @ Mon, 12 Aug 2019 21:46:36: 15000000 INFO @ Mon, 12 Aug 2019 21:46:41: 17000000 INFO @ Mon, 12 Aug 2019 21:46:44: 16000000 INFO @ Mon, 12 Aug 2019 21:46:44: 15000000 INFO @ Mon, 12 Aug 2019 21:46:48: 18000000 INFO @ Mon, 12 Aug 2019 21:46:52: 17000000 INFO @ Mon, 12 Aug 2019 21:46:53: 16000000 INFO @ Mon, 12 Aug 2019 21:46:54: 19000000 INFO @ Mon, 12 Aug 2019 21:47:00: 18000000 INFO @ Mon, 12 Aug 2019 21:47:01: 20000000 INFO @ Mon, 12 Aug 2019 21:47:01: 17000000 INFO @ Mon, 12 Aug 2019 21:47:08: 21000000 INFO @ Mon, 12 Aug 2019 21:47:08: 19000000 INFO @ Mon, 12 Aug 2019 21:47:10: 18000000 INFO @ Mon, 12 Aug 2019 21:47:15: 22000000 INFO @ Mon, 12 Aug 2019 21:47:17: 20000000 INFO @ Mon, 12 Aug 2019 21:47:19: 19000000 INFO @ Mon, 12 Aug 2019 21:47:22: 23000000 INFO @ Mon, 12 Aug 2019 21:47:25: 21000000 INFO @ Mon, 12 Aug 2019 21:47:27: 20000000 INFO @ Mon, 12 Aug 2019 21:47:28: 24000000 INFO @ Mon, 12 Aug 2019 21:47:33: 22000000 INFO @ Mon, 12 Aug 2019 21:47:35: 25000000 INFO @ Mon, 12 Aug 2019 21:47:36: 21000000 INFO @ Mon, 12 Aug 2019 21:47:41: 23000000 INFO @ Mon, 12 Aug 2019 21:47:42: 26000000 INFO @ Mon, 12 Aug 2019 21:47:45: 22000000 INFO @ Mon, 12 Aug 2019 21:47:48: 27000000 INFO @ Mon, 12 Aug 2019 21:47:49: 24000000 INFO @ Mon, 12 Aug 2019 21:47:53: 23000000 INFO @ Mon, 12 Aug 2019 21:47:55: 28000000 INFO @ Mon, 12 Aug 2019 21:47:57: 25000000 INFO @ Mon, 12 Aug 2019 21:48:02: 29000000 INFO @ Mon, 12 Aug 2019 21:48:02: 24000000 INFO @ Mon, 12 Aug 2019 21:48:05: 26000000 INFO @ Mon, 12 Aug 2019 21:48:09: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 21:48:09: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 21:48:09: #1 total tags in treatment: 29996954 INFO @ Mon, 12 Aug 2019 21:48:09: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 21:48:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 21:48:10: #1 tags after filtering in treatment: 29996954 INFO @ Mon, 12 Aug 2019 21:48:10: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 21:48:10: #1 finished! INFO @ Mon, 12 Aug 2019 21:48:10: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 21:48:10: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 21:48:11: 25000000 INFO @ Mon, 12 Aug 2019 21:48:12: #2 number of paired peaks: 29 WARNING @ Mon, 12 Aug 2019 21:48:12: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 21:48:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981669/SRX3981669.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981669/SRX3981669.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981669/SRX3981669.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981669/SRX3981669.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 21:48:13: 27000000 INFO @ Mon, 12 Aug 2019 21:48:19: 26000000 INFO @ Mon, 12 Aug 2019 21:48:22: 28000000 INFO @ Mon, 12 Aug 2019 21:48:28: 27000000 INFO @ Mon, 12 Aug 2019 21:48:30: 29000000 INFO @ Mon, 12 Aug 2019 21:48:37: 28000000 INFO @ Mon, 12 Aug 2019 21:48:38: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 21:48:38: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 21:48:38: #1 total tags in treatment: 29996954 INFO @ Mon, 12 Aug 2019 21:48:38: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 21:48:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 21:48:39: #1 tags after filtering in treatment: 29996954 INFO @ Mon, 12 Aug 2019 21:48:39: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 21:48:39: #1 finished! INFO @ Mon, 12 Aug 2019 21:48:39: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 21:48:39: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 21:48:41: #2 number of paired peaks: 29 WARNING @ Mon, 12 Aug 2019 21:48:41: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 21:48:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981669/SRX3981669.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981669/SRX3981669.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981669/SRX3981669.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981669/SRX3981669.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 21:48:45: 29000000 INFO @ Mon, 12 Aug 2019 21:48:53: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 21:48:53: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 21:48:53: #1 total tags in treatment: 29996954 INFO @ Mon, 12 Aug 2019 21:48:53: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 21:48:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 21:48:54: #1 tags after filtering in treatment: 29996954 INFO @ Mon, 12 Aug 2019 21:48:54: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 21:48:54: #1 finished! INFO @ Mon, 12 Aug 2019 21:48:54: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 21:48:54: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 21:48:57: #2 number of paired peaks: 29 WARNING @ Mon, 12 Aug 2019 21:48:57: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 21:48:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981669/SRX3981669.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981669/SRX3981669.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981669/SRX3981669.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981669/SRX3981669.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。