Job ID = 6528050 SRX = SRX3981667 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:15:05 prefetch.2.10.7: 1) Downloading 'SRR7050385'... 2020-06-29T14:15:05 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:16:08 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:16:08 prefetch.2.10.7: 'SRR7050385' is valid 2020-06-29T14:16:08 prefetch.2.10.7: 1) 'SRR7050385' was downloaded successfully 2020-06-29T14:16:08 prefetch.2.10.7: 'SRR7050385' has 0 unresolved dependencies Read 14663863 spots for SRR7050385/SRR7050385.sra Written 14663863 spots for SRR7050385/SRR7050385.sra 2020-06-29T14:17:17 prefetch.2.10.7: 1) Downloading 'SRR7050386'... 2020-06-29T14:17:17 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:18:18 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:18:19 prefetch.2.10.7: 'SRR7050386' is valid 2020-06-29T14:18:19 prefetch.2.10.7: 1) 'SRR7050386' was downloaded successfully 2020-06-29T14:18:19 prefetch.2.10.7: 'SRR7050386' has 0 unresolved dependencies Read 14647859 spots for SRR7050386/SRR7050386.sra Written 14647859 spots for SRR7050386/SRR7050386.sra 2020-06-29T14:19:31 prefetch.2.10.7: 1) Downloading 'SRR7050387'... 2020-06-29T14:19:31 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:20:44 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:20:45 prefetch.2.10.7: 'SRR7050387' is valid 2020-06-29T14:20:45 prefetch.2.10.7: 1) 'SRR7050387' was downloaded successfully 2020-06-29T14:20:45 prefetch.2.10.7: 'SRR7050387' has 0 unresolved dependencies Read 14745432 spots for SRR7050387/SRR7050387.sra Written 14745432 spots for SRR7050387/SRR7050387.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:34 44057154 reads; of these: 44057154 (100.00%) were unpaired; of these: 1952451 (4.43%) aligned 0 times 30582484 (69.42%) aligned exactly 1 time 11522219 (26.15%) aligned >1 times 95.57% overall alignment rate Time searching: 00:14:34 Overall time: 00:14:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 5271307 / 42104703 = 0.1252 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:00:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981667/SRX3981667.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981667/SRX3981667.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981667/SRX3981667.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981667/SRX3981667.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:00:19: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:00:19: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:00:25: 1000000 INFO @ Tue, 30 Jun 2020 00:00:32: 2000000 INFO @ Tue, 30 Jun 2020 00:00:38: 3000000 INFO @ Tue, 30 Jun 2020 00:00:44: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:00:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981667/SRX3981667.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981667/SRX3981667.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981667/SRX3981667.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981667/SRX3981667.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:00:49: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:00:49: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:00:50: 5000000 INFO @ Tue, 30 Jun 2020 00:00:55: 1000000 INFO @ Tue, 30 Jun 2020 00:00:56: 6000000 INFO @ Tue, 30 Jun 2020 00:01:01: 2000000 INFO @ Tue, 30 Jun 2020 00:01:03: 7000000 INFO @ Tue, 30 Jun 2020 00:01:06: 3000000 INFO @ Tue, 30 Jun 2020 00:01:09: 8000000 INFO @ Tue, 30 Jun 2020 00:01:12: 4000000 INFO @ Tue, 30 Jun 2020 00:01:15: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:01:18: 5000000 INFO @ Tue, 30 Jun 2020 00:01:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981667/SRX3981667.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981667/SRX3981667.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981667/SRX3981667.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981667/SRX3981667.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:01:20: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:01:20: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:01:22: 10000000 INFO @ Tue, 30 Jun 2020 00:01:24: 6000000 INFO @ Tue, 30 Jun 2020 00:01:26: 1000000 INFO @ Tue, 30 Jun 2020 00:01:28: 11000000 INFO @ Tue, 30 Jun 2020 00:01:31: 7000000 INFO @ Tue, 30 Jun 2020 00:01:33: 2000000 INFO @ Tue, 30 Jun 2020 00:01:34: 12000000 INFO @ Tue, 30 Jun 2020 00:01:37: 8000000 INFO @ Tue, 30 Jun 2020 00:01:39: 3000000 INFO @ Tue, 30 Jun 2020 00:01:41: 13000000 INFO @ Tue, 30 Jun 2020 00:01:44: 9000000 INFO @ Tue, 30 Jun 2020 00:01:46: 4000000 INFO @ Tue, 30 Jun 2020 00:01:47: 14000000 INFO @ Tue, 30 Jun 2020 00:01:51: 10000000 INFO @ Tue, 30 Jun 2020 00:01:53: 5000000 INFO @ Tue, 30 Jun 2020 00:01:53: 15000000 INFO @ Tue, 30 Jun 2020 00:01:57: 11000000 INFO @ Tue, 30 Jun 2020 00:01:59: 6000000 INFO @ Tue, 30 Jun 2020 00:02:00: 16000000 INFO @ Tue, 30 Jun 2020 00:02:04: 12000000 INFO @ Tue, 30 Jun 2020 00:02:06: 17000000 INFO @ Tue, 30 Jun 2020 00:02:06: 7000000 INFO @ Tue, 30 Jun 2020 00:02:10: 13000000 INFO @ Tue, 30 Jun 2020 00:02:12: 18000000 INFO @ Tue, 30 Jun 2020 00:02:13: 8000000 INFO @ Tue, 30 Jun 2020 00:02:17: 14000000 INFO @ Tue, 30 Jun 2020 00:02:18: 19000000 INFO @ Tue, 30 Jun 2020 00:02:19: 9000000 INFO @ Tue, 30 Jun 2020 00:02:24: 15000000 INFO @ Tue, 30 Jun 2020 00:02:25: 20000000 INFO @ Tue, 30 Jun 2020 00:02:26: 10000000 INFO @ Tue, 30 Jun 2020 00:02:30: 16000000 INFO @ Tue, 30 Jun 2020 00:02:31: 21000000 INFO @ Tue, 30 Jun 2020 00:02:33: 11000000 INFO @ Tue, 30 Jun 2020 00:02:37: 22000000 INFO @ Tue, 30 Jun 2020 00:02:37: 17000000 INFO @ Tue, 30 Jun 2020 00:02:39: 12000000 INFO @ Tue, 30 Jun 2020 00:02:44: 23000000 INFO @ Tue, 30 Jun 2020 00:02:44: 18000000 INFO @ Tue, 30 Jun 2020 00:02:46: 13000000 INFO @ Tue, 30 Jun 2020 00:02:50: 24000000 INFO @ Tue, 30 Jun 2020 00:02:50: 19000000 INFO @ Tue, 30 Jun 2020 00:02:53: 14000000 INFO @ Tue, 30 Jun 2020 00:02:56: 25000000 INFO @ Tue, 30 Jun 2020 00:02:57: 20000000 INFO @ Tue, 30 Jun 2020 00:02:59: 15000000 INFO @ Tue, 30 Jun 2020 00:03:03: 26000000 INFO @ Tue, 30 Jun 2020 00:03:03: 21000000 INFO @ Tue, 30 Jun 2020 00:03:06: 16000000 INFO @ Tue, 30 Jun 2020 00:03:09: 27000000 INFO @ Tue, 30 Jun 2020 00:03:09: 22000000 INFO @ Tue, 30 Jun 2020 00:03:12: 17000000 INFO @ Tue, 30 Jun 2020 00:03:15: 28000000 INFO @ Tue, 30 Jun 2020 00:03:16: 23000000 INFO @ Tue, 30 Jun 2020 00:03:19: 18000000 INFO @ Tue, 30 Jun 2020 00:03:22: 29000000 INFO @ Tue, 30 Jun 2020 00:03:22: 24000000 INFO @ Tue, 30 Jun 2020 00:03:25: 19000000 INFO @ Tue, 30 Jun 2020 00:03:28: 30000000 INFO @ Tue, 30 Jun 2020 00:03:29: 25000000 INFO @ Tue, 30 Jun 2020 00:03:32: 20000000 INFO @ Tue, 30 Jun 2020 00:03:34: 31000000 INFO @ Tue, 30 Jun 2020 00:03:35: 26000000 INFO @ Tue, 30 Jun 2020 00:03:38: 21000000 INFO @ Tue, 30 Jun 2020 00:03:40: 32000000 INFO @ Tue, 30 Jun 2020 00:03:42: 27000000 INFO @ Tue, 30 Jun 2020 00:03:45: 22000000 INFO @ Tue, 30 Jun 2020 00:03:47: 33000000 INFO @ Tue, 30 Jun 2020 00:03:49: 28000000 INFO @ Tue, 30 Jun 2020 00:03:51: 23000000 INFO @ Tue, 30 Jun 2020 00:03:53: 34000000 INFO @ Tue, 30 Jun 2020 00:03:55: 29000000 INFO @ Tue, 30 Jun 2020 00:03:58: 24000000 INFO @ Tue, 30 Jun 2020 00:03:59: 35000000 INFO @ Tue, 30 Jun 2020 00:04:02: 30000000 INFO @ Tue, 30 Jun 2020 00:04:04: 25000000 INFO @ Tue, 30 Jun 2020 00:04:05: 36000000 INFO @ Tue, 30 Jun 2020 00:04:09: 31000000 INFO @ Tue, 30 Jun 2020 00:04:11: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:04:11: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:04:11: #1 total tags in treatment: 36833396 INFO @ Tue, 30 Jun 2020 00:04:11: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:04:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:04:11: 26000000 INFO @ Tue, 30 Jun 2020 00:04:11: #1 tags after filtering in treatment: 36833396 INFO @ Tue, 30 Jun 2020 00:04:11: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:04:11: #1 finished! INFO @ Tue, 30 Jun 2020 00:04:11: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:04:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:04:14: #2 number of paired peaks: 14 WARNING @ Tue, 30 Jun 2020 00:04:14: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:04:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981667/SRX3981667.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981667/SRX3981667.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981667/SRX3981667.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981667/SRX3981667.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:04:15: 32000000 INFO @ Tue, 30 Jun 2020 00:04:17: 27000000 INFO @ Tue, 30 Jun 2020 00:04:22: 33000000 INFO @ Tue, 30 Jun 2020 00:04:24: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:04:28: 34000000 INFO @ Tue, 30 Jun 2020 00:04:30: 29000000 INFO @ Tue, 30 Jun 2020 00:04:35: 35000000 INFO @ Tue, 30 Jun 2020 00:04:37: 30000000 INFO @ Tue, 30 Jun 2020 00:04:41: 36000000 INFO @ Tue, 30 Jun 2020 00:04:44: 31000000 INFO @ Tue, 30 Jun 2020 00:04:47: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:04:47: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:04:47: #1 total tags in treatment: 36833396 INFO @ Tue, 30 Jun 2020 00:04:47: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:04:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:04:47: #1 tags after filtering in treatment: 36833396 INFO @ Tue, 30 Jun 2020 00:04:47: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:04:47: #1 finished! INFO @ Tue, 30 Jun 2020 00:04:47: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:04:47: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:04:50: #2 number of paired peaks: 14 WARNING @ Tue, 30 Jun 2020 00:04:50: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:04:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981667/SRX3981667.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981667/SRX3981667.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981667/SRX3981667.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981667/SRX3981667.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:04:50: 32000000 INFO @ Tue, 30 Jun 2020 00:04:56: 33000000 INFO @ Tue, 30 Jun 2020 00:05:02: 34000000 INFO @ Tue, 30 Jun 2020 00:05:07: 35000000 INFO @ Tue, 30 Jun 2020 00:05:13: 36000000 INFO @ Tue, 30 Jun 2020 00:05:18: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:05:18: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:05:18: #1 total tags in treatment: 36833396 INFO @ Tue, 30 Jun 2020 00:05:18: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:05:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:05:19: #1 tags after filtering in treatment: 36833396 INFO @ Tue, 30 Jun 2020 00:05:19: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:05:19: #1 finished! INFO @ Tue, 30 Jun 2020 00:05:19: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:05:19: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:05:21: #2 number of paired peaks: 14 WARNING @ Tue, 30 Jun 2020 00:05:21: Too few paired peaks (14) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:05:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981667/SRX3981667.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981667/SRX3981667.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981667/SRX3981667.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981667/SRX3981667.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。