Job ID = 6528046 SRX = SRX3981664 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:03:36 prefetch.2.10.7: 1) Downloading 'SRR7050376'... 2020-06-29T14:03:36 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:05:22 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:05:22 prefetch.2.10.7: 'SRR7050376' is valid 2020-06-29T14:05:22 prefetch.2.10.7: 1) 'SRR7050376' was downloaded successfully 2020-06-29T14:05:22 prefetch.2.10.7: 'SRR7050376' has 0 unresolved dependencies Read 12452896 spots for SRR7050376/SRR7050376.sra Written 12452896 spots for SRR7050376/SRR7050376.sra 2020-06-29T14:06:14 prefetch.2.10.7: 1) Downloading 'SRR7050377'... 2020-06-29T14:06:14 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:07:40 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:07:40 prefetch.2.10.7: 'SRR7050377' is valid 2020-06-29T14:07:40 prefetch.2.10.7: 1) 'SRR7050377' was downloaded successfully 2020-06-29T14:07:40 prefetch.2.10.7: 'SRR7050377' has 0 unresolved dependencies Read 12443735 spots for SRR7050377/SRR7050377.sra Written 12443735 spots for SRR7050377/SRR7050377.sra 2020-06-29T14:08:31 prefetch.2.10.7: 1) Downloading 'SRR7050378'... 2020-06-29T14:08:31 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:10:06 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:10:06 prefetch.2.10.7: 'SRR7050378' is valid 2020-06-29T14:10:06 prefetch.2.10.7: 1) 'SRR7050378' was downloaded successfully 2020-06-29T14:10:06 prefetch.2.10.7: 'SRR7050378' has 0 unresolved dependencies Read 12526554 spots for SRR7050378/SRR7050378.sra Written 12526554 spots for SRR7050378/SRR7050378.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:37 37423185 reads; of these: 37423185 (100.00%) were unpaired; of these: 1402595 (3.75%) aligned 0 times 26637375 (71.18%) aligned exactly 1 time 9383215 (25.07%) aligned >1 times 96.25% overall alignment rate Time searching: 00:11:37 Overall time: 00:11:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 3778796 / 36020590 = 0.1049 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:38:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981664/SRX3981664.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981664/SRX3981664.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981664/SRX3981664.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981664/SRX3981664.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:38:01: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:38:01: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:38:06: 1000000 INFO @ Mon, 29 Jun 2020 23:38:11: 2000000 INFO @ Mon, 29 Jun 2020 23:38:16: 3000000 INFO @ Mon, 29 Jun 2020 23:38:20: 4000000 INFO @ Mon, 29 Jun 2020 23:38:25: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:38:30: 6000000 INFO @ Mon, 29 Jun 2020 23:38:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981664/SRX3981664.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981664/SRX3981664.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981664/SRX3981664.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981664/SRX3981664.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:38:31: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:38:31: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:38:35: 7000000 INFO @ Mon, 29 Jun 2020 23:38:36: 1000000 INFO @ Mon, 29 Jun 2020 23:38:39: 8000000 INFO @ Mon, 29 Jun 2020 23:38:41: 2000000 INFO @ Mon, 29 Jun 2020 23:38:44: 9000000 INFO @ Mon, 29 Jun 2020 23:38:46: 3000000 INFO @ Mon, 29 Jun 2020 23:38:49: 10000000 INFO @ Mon, 29 Jun 2020 23:38:51: 4000000 INFO @ Mon, 29 Jun 2020 23:38:54: 11000000 INFO @ Mon, 29 Jun 2020 23:38:55: 5000000 INFO @ Mon, 29 Jun 2020 23:38:59: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:39:00: 6000000 INFO @ Mon, 29 Jun 2020 23:39:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981664/SRX3981664.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981664/SRX3981664.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981664/SRX3981664.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981664/SRX3981664.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:39:01: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:39:01: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:39:04: 13000000 INFO @ Mon, 29 Jun 2020 23:39:05: 7000000 INFO @ Mon, 29 Jun 2020 23:39:07: 1000000 INFO @ Mon, 29 Jun 2020 23:39:09: 14000000 INFO @ Mon, 29 Jun 2020 23:39:10: 8000000 INFO @ Mon, 29 Jun 2020 23:39:12: 2000000 INFO @ Mon, 29 Jun 2020 23:39:14: 15000000 INFO @ Mon, 29 Jun 2020 23:39:15: 9000000 INFO @ Mon, 29 Jun 2020 23:39:17: 3000000 INFO @ Mon, 29 Jun 2020 23:39:19: 16000000 INFO @ Mon, 29 Jun 2020 23:39:20: 10000000 INFO @ Mon, 29 Jun 2020 23:39:22: 4000000 INFO @ Mon, 29 Jun 2020 23:39:24: 17000000 INFO @ Mon, 29 Jun 2020 23:39:26: 11000000 INFO @ Mon, 29 Jun 2020 23:39:27: 5000000 INFO @ Mon, 29 Jun 2020 23:39:29: 18000000 INFO @ Mon, 29 Jun 2020 23:39:31: 12000000 INFO @ Mon, 29 Jun 2020 23:39:32: 6000000 INFO @ Mon, 29 Jun 2020 23:39:34: 19000000 INFO @ Mon, 29 Jun 2020 23:39:36: 13000000 INFO @ Mon, 29 Jun 2020 23:39:37: 7000000 INFO @ Mon, 29 Jun 2020 23:39:39: 20000000 INFO @ Mon, 29 Jun 2020 23:39:41: 14000000 INFO @ Mon, 29 Jun 2020 23:39:42: 8000000 INFO @ Mon, 29 Jun 2020 23:39:44: 21000000 INFO @ Mon, 29 Jun 2020 23:39:46: 15000000 INFO @ Mon, 29 Jun 2020 23:39:47: 9000000 INFO @ Mon, 29 Jun 2020 23:39:49: 22000000 INFO @ Mon, 29 Jun 2020 23:39:51: 16000000 INFO @ Mon, 29 Jun 2020 23:39:52: 10000000 INFO @ Mon, 29 Jun 2020 23:39:54: 23000000 INFO @ Mon, 29 Jun 2020 23:39:56: 17000000 INFO @ Mon, 29 Jun 2020 23:39:57: 11000000 INFO @ Mon, 29 Jun 2020 23:40:00: 24000000 INFO @ Mon, 29 Jun 2020 23:40:01: 18000000 INFO @ Mon, 29 Jun 2020 23:40:02: 12000000 INFO @ Mon, 29 Jun 2020 23:40:05: 25000000 INFO @ Mon, 29 Jun 2020 23:40:06: 19000000 INFO @ Mon, 29 Jun 2020 23:40:07: 13000000 INFO @ Mon, 29 Jun 2020 23:40:10: 26000000 INFO @ Mon, 29 Jun 2020 23:40:11: 20000000 INFO @ Mon, 29 Jun 2020 23:40:12: 14000000 INFO @ Mon, 29 Jun 2020 23:40:15: 27000000 INFO @ Mon, 29 Jun 2020 23:40:16: 21000000 INFO @ Mon, 29 Jun 2020 23:40:17: 15000000 INFO @ Mon, 29 Jun 2020 23:40:20: 28000000 INFO @ Mon, 29 Jun 2020 23:40:21: 22000000 INFO @ Mon, 29 Jun 2020 23:40:22: 16000000 INFO @ Mon, 29 Jun 2020 23:40:26: 29000000 INFO @ Mon, 29 Jun 2020 23:40:26: 23000000 INFO @ Mon, 29 Jun 2020 23:40:28: 17000000 INFO @ Mon, 29 Jun 2020 23:40:31: 30000000 INFO @ Mon, 29 Jun 2020 23:40:31: 24000000 INFO @ Mon, 29 Jun 2020 23:40:33: 18000000 INFO @ Mon, 29 Jun 2020 23:40:36: 31000000 INFO @ Mon, 29 Jun 2020 23:40:36: 25000000 INFO @ Mon, 29 Jun 2020 23:40:38: 19000000 INFO @ Mon, 29 Jun 2020 23:40:41: 32000000 INFO @ Mon, 29 Jun 2020 23:40:41: 26000000 INFO @ Mon, 29 Jun 2020 23:40:42: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:40:42: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:40:42: #1 total tags in treatment: 32241794 INFO @ Mon, 29 Jun 2020 23:40:42: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:40:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:40:43: 20000000 INFO @ Mon, 29 Jun 2020 23:40:43: #1 tags after filtering in treatment: 32241794 INFO @ Mon, 29 Jun 2020 23:40:43: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:40:43: #1 finished! INFO @ Mon, 29 Jun 2020 23:40:43: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:40:43: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:40:45: #2 number of paired peaks: 20 WARNING @ Mon, 29 Jun 2020 23:40:45: Too few paired peaks (20) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:40:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981664/SRX3981664.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981664/SRX3981664.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981664/SRX3981664.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981664/SRX3981664.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:40:46: 27000000 INFO @ Mon, 29 Jun 2020 23:40:48: 21000000 INFO @ Mon, 29 Jun 2020 23:40:52: 28000000 INFO @ Mon, 29 Jun 2020 23:40:52: 22000000 INFO @ Mon, 29 Jun 2020 23:40:57: 29000000 INFO @ Mon, 29 Jun 2020 23:40:57: 23000000 INFO @ Mon, 29 Jun 2020 23:41:02: 30000000 INFO @ Mon, 29 Jun 2020 23:41:02: 24000000 INFO @ Mon, 29 Jun 2020 23:41:07: 31000000 INFO @ Mon, 29 Jun 2020 23:41:07: 25000000 INFO @ Mon, 29 Jun 2020 23:41:12: 32000000 INFO @ Mon, 29 Jun 2020 23:41:12: 26000000 INFO @ Mon, 29 Jun 2020 23:41:13: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:41:13: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:41:13: #1 total tags in treatment: 32241794 INFO @ Mon, 29 Jun 2020 23:41:13: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:41:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:41:13: #1 tags after filtering in treatment: 32241794 INFO @ Mon, 29 Jun 2020 23:41:13: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:41:13: #1 finished! INFO @ Mon, 29 Jun 2020 23:41:13: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:41:13: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:41:15: #2 number of paired peaks: 20 WARNING @ Mon, 29 Jun 2020 23:41:15: Too few paired peaks (20) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:41:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981664/SRX3981664.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981664/SRX3981664.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981664/SRX3981664.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981664/SRX3981664.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:41:17: 27000000 INFO @ Mon, 29 Jun 2020 23:41:22: 28000000 INFO @ Mon, 29 Jun 2020 23:41:27: 29000000 INFO @ Mon, 29 Jun 2020 23:41:32: 30000000 INFO @ Mon, 29 Jun 2020 23:41:37: 31000000 INFO @ Mon, 29 Jun 2020 23:41:42: 32000000 INFO @ Mon, 29 Jun 2020 23:41:43: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:41:43: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:41:43: #1 total tags in treatment: 32241794 INFO @ Mon, 29 Jun 2020 23:41:43: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:41:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:41:44: #1 tags after filtering in treatment: 32241794 INFO @ Mon, 29 Jun 2020 23:41:44: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:41:44: #1 finished! INFO @ Mon, 29 Jun 2020 23:41:44: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:41:44: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:41:46: #2 number of paired peaks: 20 WARNING @ Mon, 29 Jun 2020 23:41:46: Too few paired peaks (20) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:41:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981664/SRX3981664.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981664/SRX3981664.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981664/SRX3981664.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981664/SRX3981664.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。