Job ID = 6528045 SRX = SRX3981663 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:47:13 prefetch.2.10.7: 1) Downloading 'SRR7050373'... 2020-06-29T14:47:13 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:48:04 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:48:05 prefetch.2.10.7: 'SRR7050373' is valid 2020-06-29T14:48:05 prefetch.2.10.7: 1) 'SRR7050373' was downloaded successfully 2020-06-29T14:48:05 prefetch.2.10.7: 'SRR7050373' has 0 unresolved dependencies Read 14977375 spots for SRR7050373/SRR7050373.sra Written 14977375 spots for SRR7050373/SRR7050373.sra 2020-06-29T14:49:15 prefetch.2.10.7: 1) Downloading 'SRR7050374'... 2020-06-29T14:49:15 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:50:34 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:50:34 prefetch.2.10.7: 'SRR7050374' is valid 2020-06-29T14:50:34 prefetch.2.10.7: 1) 'SRR7050374' was downloaded successfully 2020-06-29T14:50:34 prefetch.2.10.7: 'SRR7050374' has 0 unresolved dependencies Read 14982070 spots for SRR7050374/SRR7050374.sra Written 14982070 spots for SRR7050374/SRR7050374.sra 2020-06-29T14:51:46 prefetch.2.10.7: 1) Downloading 'SRR7050375'... 2020-06-29T14:51:46 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:52:50 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:52:51 prefetch.2.10.7: 'SRR7050375' is valid 2020-06-29T14:52:51 prefetch.2.10.7: 1) 'SRR7050375' was downloaded successfully 2020-06-29T14:52:51 prefetch.2.10.7: 'SRR7050375' has 0 unresolved dependencies Read 15084770 spots for SRR7050375/SRR7050375.sra Written 15084770 spots for SRR7050375/SRR7050375.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:38 45044215 reads; of these: 45044215 (100.00%) were unpaired; of these: 1898843 (4.22%) aligned 0 times 31992055 (71.02%) aligned exactly 1 time 11153317 (24.76%) aligned >1 times 95.78% overall alignment rate Time searching: 00:13:38 Overall time: 00:13:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 5013458 / 43145372 = 0.1162 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:32:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981663/SRX3981663.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981663/SRX3981663.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981663/SRX3981663.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981663/SRX3981663.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:32:01: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:32:01: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:32:06: 1000000 INFO @ Tue, 30 Jun 2020 00:32:11: 2000000 INFO @ Tue, 30 Jun 2020 00:32:16: 3000000 INFO @ Tue, 30 Jun 2020 00:32:21: 4000000 INFO @ Tue, 30 Jun 2020 00:32:26: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:32:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981663/SRX3981663.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981663/SRX3981663.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981663/SRX3981663.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981663/SRX3981663.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:32:31: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:32:31: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:32:32: 6000000 INFO @ Tue, 30 Jun 2020 00:32:37: 7000000 INFO @ Tue, 30 Jun 2020 00:32:37: 1000000 INFO @ Tue, 30 Jun 2020 00:32:42: 8000000 INFO @ Tue, 30 Jun 2020 00:32:42: 2000000 INFO @ Tue, 30 Jun 2020 00:32:47: 9000000 INFO @ Tue, 30 Jun 2020 00:32:48: 3000000 INFO @ Tue, 30 Jun 2020 00:32:52: 10000000 INFO @ Tue, 30 Jun 2020 00:32:54: 4000000 INFO @ Tue, 30 Jun 2020 00:32:57: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:33:00: 5000000 INFO @ Tue, 30 Jun 2020 00:33:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981663/SRX3981663.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981663/SRX3981663.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981663/SRX3981663.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981663/SRX3981663.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:33:01: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:33:01: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:33:02: 12000000 INFO @ Tue, 30 Jun 2020 00:33:05: 6000000 INFO @ Tue, 30 Jun 2020 00:33:07: 1000000 INFO @ Tue, 30 Jun 2020 00:33:07: 13000000 INFO @ Tue, 30 Jun 2020 00:33:11: 7000000 INFO @ Tue, 30 Jun 2020 00:33:12: 14000000 INFO @ Tue, 30 Jun 2020 00:33:13: 2000000 INFO @ Tue, 30 Jun 2020 00:33:17: 8000000 INFO @ Tue, 30 Jun 2020 00:33:18: 15000000 INFO @ Tue, 30 Jun 2020 00:33:19: 3000000 INFO @ Tue, 30 Jun 2020 00:33:23: 16000000 INFO @ Tue, 30 Jun 2020 00:33:23: 9000000 INFO @ Tue, 30 Jun 2020 00:33:25: 4000000 INFO @ Tue, 30 Jun 2020 00:33:29: 17000000 INFO @ Tue, 30 Jun 2020 00:33:30: 10000000 INFO @ Tue, 30 Jun 2020 00:33:31: 5000000 INFO @ Tue, 30 Jun 2020 00:33:34: 18000000 INFO @ Tue, 30 Jun 2020 00:33:36: 11000000 INFO @ Tue, 30 Jun 2020 00:33:37: 6000000 INFO @ Tue, 30 Jun 2020 00:33:39: 19000000 INFO @ Tue, 30 Jun 2020 00:33:42: 12000000 INFO @ Tue, 30 Jun 2020 00:33:43: 7000000 INFO @ Tue, 30 Jun 2020 00:33:45: 20000000 INFO @ Tue, 30 Jun 2020 00:33:48: 13000000 INFO @ Tue, 30 Jun 2020 00:33:49: 8000000 INFO @ Tue, 30 Jun 2020 00:33:50: 21000000 INFO @ Tue, 30 Jun 2020 00:33:54: 14000000 INFO @ Tue, 30 Jun 2020 00:33:55: 9000000 INFO @ Tue, 30 Jun 2020 00:33:55: 22000000 INFO @ Tue, 30 Jun 2020 00:34:00: 15000000 INFO @ Tue, 30 Jun 2020 00:34:00: 23000000 INFO @ Tue, 30 Jun 2020 00:34:01: 10000000 INFO @ Tue, 30 Jun 2020 00:34:06: 24000000 INFO @ Tue, 30 Jun 2020 00:34:06: 16000000 INFO @ Tue, 30 Jun 2020 00:34:07: 11000000 INFO @ Tue, 30 Jun 2020 00:34:11: 25000000 INFO @ Tue, 30 Jun 2020 00:34:12: 17000000 INFO @ Tue, 30 Jun 2020 00:34:13: 12000000 INFO @ Tue, 30 Jun 2020 00:34:16: 26000000 INFO @ Tue, 30 Jun 2020 00:34:18: 18000000 INFO @ Tue, 30 Jun 2020 00:34:19: 13000000 INFO @ Tue, 30 Jun 2020 00:34:21: 27000000 INFO @ Tue, 30 Jun 2020 00:34:24: 19000000 INFO @ Tue, 30 Jun 2020 00:34:25: 14000000 INFO @ Tue, 30 Jun 2020 00:34:27: 28000000 INFO @ Tue, 30 Jun 2020 00:34:30: 20000000 INFO @ Tue, 30 Jun 2020 00:34:31: 15000000 INFO @ Tue, 30 Jun 2020 00:34:32: 29000000 INFO @ Tue, 30 Jun 2020 00:34:36: 21000000 INFO @ Tue, 30 Jun 2020 00:34:37: 16000000 INFO @ Tue, 30 Jun 2020 00:34:37: 30000000 INFO @ Tue, 30 Jun 2020 00:34:42: 22000000 INFO @ Tue, 30 Jun 2020 00:34:43: 31000000 INFO @ Tue, 30 Jun 2020 00:34:43: 17000000 INFO @ Tue, 30 Jun 2020 00:34:47: 23000000 INFO @ Tue, 30 Jun 2020 00:34:48: 32000000 INFO @ Tue, 30 Jun 2020 00:34:49: 18000000 INFO @ Tue, 30 Jun 2020 00:34:53: 24000000 INFO @ Tue, 30 Jun 2020 00:34:53: 33000000 INFO @ Tue, 30 Jun 2020 00:34:55: 19000000 INFO @ Tue, 30 Jun 2020 00:34:59: 34000000 INFO @ Tue, 30 Jun 2020 00:34:59: 25000000 INFO @ Tue, 30 Jun 2020 00:35:01: 20000000 INFO @ Tue, 30 Jun 2020 00:35:04: 35000000 INFO @ Tue, 30 Jun 2020 00:35:05: 26000000 INFO @ Tue, 30 Jun 2020 00:35:07: 21000000 INFO @ Tue, 30 Jun 2020 00:35:10: 36000000 INFO @ Tue, 30 Jun 2020 00:35:11: 27000000 INFO @ Tue, 30 Jun 2020 00:35:13: 22000000 INFO @ Tue, 30 Jun 2020 00:35:15: 37000000 INFO @ Tue, 30 Jun 2020 00:35:17: 28000000 INFO @ Tue, 30 Jun 2020 00:35:19: 23000000 INFO @ Tue, 30 Jun 2020 00:35:20: 38000000 INFO @ Tue, 30 Jun 2020 00:35:21: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:35:21: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:35:21: #1 total tags in treatment: 38131914 INFO @ Tue, 30 Jun 2020 00:35:21: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:35:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:35:22: #1 tags after filtering in treatment: 38131914 INFO @ Tue, 30 Jun 2020 00:35:22: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:35:22: #1 finished! INFO @ Tue, 30 Jun 2020 00:35:22: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:35:22: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:35:23: 29000000 INFO @ Tue, 30 Jun 2020 00:35:25: #2 number of paired peaks: 3 WARNING @ Tue, 30 Jun 2020 00:35:25: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:35:25: Process for pairing-model is terminated! INFO @ Tue, 30 Jun 2020 00:35:25: 24000000 cut: /home/okishinya/chipatlas/results/dm3/SRX3981663/SRX3981663.05_peaks.narrowPeak: No such file or directory INFO @ Tue, 30 Jun 2020 00:35:29: 30000000 INFO @ Tue, 30 Jun 2020 00:35:31: 25000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981663/SRX3981663.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981663/SRX3981663.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981663/SRX3981663.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:35:35: 31000000 INFO @ Tue, 30 Jun 2020 00:35:36: 26000000 INFO @ Tue, 30 Jun 2020 00:35:41: 32000000 INFO @ Tue, 30 Jun 2020 00:35:42: 27000000 INFO @ Tue, 30 Jun 2020 00:35:47: 33000000 INFO @ Tue, 30 Jun 2020 00:35:48: 28000000 INFO @ Tue, 30 Jun 2020 00:35:53: 34000000 INFO @ Tue, 30 Jun 2020 00:35:54: 29000000 INFO @ Tue, 30 Jun 2020 00:35:59: 35000000 INFO @ Tue, 30 Jun 2020 00:36:00: 30000000 INFO @ Tue, 30 Jun 2020 00:36:05: 36000000 INFO @ Tue, 30 Jun 2020 00:36:06: 31000000 INFO @ Tue, 30 Jun 2020 00:36:10: 37000000 INFO @ Tue, 30 Jun 2020 00:36:12: 32000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:36:16: 38000000 INFO @ Tue, 30 Jun 2020 00:36:17: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:36:17: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:36:17: #1 total tags in treatment: 38131914 INFO @ Tue, 30 Jun 2020 00:36:17: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:36:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:36:18: 33000000 INFO @ Tue, 30 Jun 2020 00:36:18: #1 tags after filtering in treatment: 38131914 INFO @ Tue, 30 Jun 2020 00:36:18: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:36:18: #1 finished! INFO @ Tue, 30 Jun 2020 00:36:18: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:36:18: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:36:21: #2 number of paired peaks: 3 WARNING @ Tue, 30 Jun 2020 00:36:21: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:36:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981663/SRX3981663.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis INFO @ Tue, 30 Jun 2020 00:36:23: 34000000 needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981663/SRX3981663.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981663/SRX3981663.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981663/SRX3981663.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:36:28: 35000000 INFO @ Tue, 30 Jun 2020 00:36:34: 36000000 INFO @ Tue, 30 Jun 2020 00:36:39: 37000000 INFO @ Tue, 30 Jun 2020 00:36:44: 38000000 INFO @ Tue, 30 Jun 2020 00:36:45: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:36:45: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:36:45: #1 total tags in treatment: 38131914 INFO @ Tue, 30 Jun 2020 00:36:45: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:36:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:36:46: #1 tags after filtering in treatment: 38131914 INFO @ Tue, 30 Jun 2020 00:36:46: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:36:46: #1 finished! INFO @ Tue, 30 Jun 2020 00:36:46: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:36:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:36:48: #2 number of paired peaks: 3 WARNING @ Tue, 30 Jun 2020 00:36:48: Too few paired peaks (3) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:36:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981663/SRX3981663.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981663/SRX3981663.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981663/SRX3981663.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981663/SRX3981663.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。