Job ID = 6528044 SRX = SRX3981662 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:04:06 prefetch.2.10.7: 1) Downloading 'SRR7050370'... 2020-06-29T14:04:06 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:05:44 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:05:44 prefetch.2.10.7: 'SRR7050370' is valid 2020-06-29T14:05:44 prefetch.2.10.7: 1) 'SRR7050370' was downloaded successfully 2020-06-29T14:05:44 prefetch.2.10.7: 'SRR7050370' has 0 unresolved dependencies Read 14154485 spots for SRR7050370/SRR7050370.sra Written 14154485 spots for SRR7050370/SRR7050370.sra 2020-06-29T14:06:51 prefetch.2.10.7: 1) Downloading 'SRR7050371'... 2020-06-29T14:06:51 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:08:10 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:08:11 prefetch.2.10.7: 'SRR7050371' is valid 2020-06-29T14:08:11 prefetch.2.10.7: 1) 'SRR7050371' was downloaded successfully 2020-06-29T14:08:11 prefetch.2.10.7: 'SRR7050371' has 0 unresolved dependencies Read 14132721 spots for SRR7050371/SRR7050371.sra Written 14132721 spots for SRR7050371/SRR7050371.sra 2020-06-29T14:09:19 prefetch.2.10.7: 1) Downloading 'SRR7050372'... 2020-06-29T14:09:19 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:16:43 prefetch.2.10.7: HTTPS download failed 2020-06-29T14:16:43 prefetch.2.10.7: 1) failed to download SRR7050372 2020-06-29T14:16:56 prefetch.2.10.7: 1) Downloading 'SRR7050372'... 2020-06-29T14:16:57 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:16:57 prefetch.2.10.7: Continue download of 'SRR7050372' from 274861371 2020-06-29T14:17:05 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:17:06 prefetch.2.10.7: 'SRR7050372' is valid 2020-06-29T14:17:06 prefetch.2.10.7: 1) 'SRR7050372' was downloaded successfully 2020-06-29T14:17:06 prefetch.2.10.7: 'SRR7050372' has 0 unresolved dependencies Read 14225762 spots for SRR7050372/SRR7050372.sra Written 14225762 spots for SRR7050372/SRR7050372.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:29 42512968 reads; of these: 42512968 (100.00%) were unpaired; of these: 2572903 (6.05%) aligned 0 times 29632140 (69.70%) aligned exactly 1 time 10307925 (24.25%) aligned >1 times 93.95% overall alignment rate Time searching: 00:12:29 Overall time: 00:12:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 4376526 / 39940065 = 0.1096 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:53:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981662/SRX3981662.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981662/SRX3981662.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981662/SRX3981662.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981662/SRX3981662.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:53:30: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:53:30: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:53:37: 1000000 INFO @ Mon, 29 Jun 2020 23:53:43: 2000000 INFO @ Mon, 29 Jun 2020 23:53:49: 3000000 INFO @ Mon, 29 Jun 2020 23:53:55: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:54:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981662/SRX3981662.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981662/SRX3981662.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981662/SRX3981662.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981662/SRX3981662.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:54:00: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:54:00: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:54:02: 5000000 INFO @ Mon, 29 Jun 2020 23:54:08: 1000000 INFO @ Mon, 29 Jun 2020 23:54:10: 6000000 INFO @ Mon, 29 Jun 2020 23:54:15: 2000000 INFO @ Mon, 29 Jun 2020 23:54:17: 7000000 INFO @ Mon, 29 Jun 2020 23:54:23: 3000000 INFO @ Mon, 29 Jun 2020 23:54:24: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:54:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981662/SRX3981662.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981662/SRX3981662.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981662/SRX3981662.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981662/SRX3981662.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:54:30: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:54:30: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:54:30: 4000000 INFO @ Mon, 29 Jun 2020 23:54:32: 9000000 INFO @ Mon, 29 Jun 2020 23:54:37: 1000000 INFO @ Mon, 29 Jun 2020 23:54:38: 5000000 INFO @ Mon, 29 Jun 2020 23:54:40: 10000000 INFO @ Mon, 29 Jun 2020 23:54:44: 2000000 INFO @ Mon, 29 Jun 2020 23:54:47: 6000000 INFO @ Mon, 29 Jun 2020 23:54:47: 11000000 INFO @ Mon, 29 Jun 2020 23:54:50: 3000000 INFO @ Mon, 29 Jun 2020 23:54:55: 7000000 INFO @ Mon, 29 Jun 2020 23:54:55: 12000000 INFO @ Mon, 29 Jun 2020 23:54:57: 4000000 INFO @ Mon, 29 Jun 2020 23:55:03: 13000000 INFO @ Mon, 29 Jun 2020 23:55:03: 8000000 INFO @ Mon, 29 Jun 2020 23:55:05: 5000000 INFO @ Mon, 29 Jun 2020 23:55:12: 14000000 INFO @ Mon, 29 Jun 2020 23:55:12: 9000000 INFO @ Mon, 29 Jun 2020 23:55:13: 6000000 INFO @ Mon, 29 Jun 2020 23:55:21: 7000000 INFO @ Mon, 29 Jun 2020 23:55:21: 15000000 INFO @ Mon, 29 Jun 2020 23:55:21: 10000000 INFO @ Mon, 29 Jun 2020 23:55:29: 8000000 INFO @ Mon, 29 Jun 2020 23:55:30: 16000000 INFO @ Mon, 29 Jun 2020 23:55:30: 11000000 INFO @ Mon, 29 Jun 2020 23:55:36: 9000000 INFO @ Mon, 29 Jun 2020 23:55:38: 12000000 INFO @ Mon, 29 Jun 2020 23:55:38: 17000000 INFO @ Mon, 29 Jun 2020 23:55:44: 10000000 INFO @ Mon, 29 Jun 2020 23:55:47: 13000000 INFO @ Mon, 29 Jun 2020 23:55:48: 18000000 INFO @ Mon, 29 Jun 2020 23:55:52: 11000000 INFO @ Mon, 29 Jun 2020 23:55:57: 14000000 INFO @ Mon, 29 Jun 2020 23:55:57: 19000000 INFO @ Mon, 29 Jun 2020 23:56:00: 12000000 INFO @ Mon, 29 Jun 2020 23:56:07: 15000000 INFO @ Mon, 29 Jun 2020 23:56:07: 20000000 INFO @ Mon, 29 Jun 2020 23:56:08: 13000000 INFO @ Mon, 29 Jun 2020 23:56:16: 14000000 INFO @ Mon, 29 Jun 2020 23:56:16: 16000000 INFO @ Mon, 29 Jun 2020 23:56:16: 21000000 INFO @ Mon, 29 Jun 2020 23:56:23: 15000000 INFO @ Mon, 29 Jun 2020 23:56:26: 22000000 INFO @ Mon, 29 Jun 2020 23:56:26: 17000000 INFO @ Mon, 29 Jun 2020 23:56:31: 16000000 INFO @ Mon, 29 Jun 2020 23:56:35: 23000000 INFO @ Mon, 29 Jun 2020 23:56:36: 18000000 INFO @ Mon, 29 Jun 2020 23:56:40: 17000000 INFO @ Mon, 29 Jun 2020 23:56:45: 24000000 INFO @ Mon, 29 Jun 2020 23:56:46: 19000000 INFO @ Mon, 29 Jun 2020 23:56:48: 18000000 INFO @ Mon, 29 Jun 2020 23:56:55: 25000000 INFO @ Mon, 29 Jun 2020 23:56:56: 20000000 INFO @ Mon, 29 Jun 2020 23:56:56: 19000000 INFO @ Mon, 29 Jun 2020 23:57:04: 26000000 INFO @ Mon, 29 Jun 2020 23:57:04: 20000000 INFO @ Mon, 29 Jun 2020 23:57:05: 21000000 INFO @ Mon, 29 Jun 2020 23:57:14: 27000000 INFO @ Mon, 29 Jun 2020 23:57:14: 21000000 INFO @ Mon, 29 Jun 2020 23:57:15: 22000000 INFO @ Mon, 29 Jun 2020 23:57:23: 22000000 INFO @ Mon, 29 Jun 2020 23:57:24: 28000000 INFO @ Mon, 29 Jun 2020 23:57:25: 23000000 INFO @ Mon, 29 Jun 2020 23:57:32: 23000000 INFO @ Mon, 29 Jun 2020 23:57:33: 29000000 INFO @ Mon, 29 Jun 2020 23:57:34: 24000000 INFO @ Mon, 29 Jun 2020 23:57:42: 24000000 INFO @ Mon, 29 Jun 2020 23:57:43: 30000000 INFO @ Mon, 29 Jun 2020 23:57:43: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:57:51: 25000000 INFO @ Mon, 29 Jun 2020 23:57:52: 31000000 INFO @ Mon, 29 Jun 2020 23:57:53: 26000000 INFO @ Mon, 29 Jun 2020 23:58:00: 32000000 INFO @ Mon, 29 Jun 2020 23:58:00: 26000000 INFO @ Mon, 29 Jun 2020 23:58:02: 27000000 INFO @ Mon, 29 Jun 2020 23:58:09: 33000000 INFO @ Mon, 29 Jun 2020 23:58:10: 27000000 INFO @ Mon, 29 Jun 2020 23:58:11: 28000000 INFO @ Mon, 29 Jun 2020 23:58:18: 34000000 INFO @ Mon, 29 Jun 2020 23:58:19: 28000000 INFO @ Mon, 29 Jun 2020 23:58:20: 29000000 INFO @ Mon, 29 Jun 2020 23:58:27: 35000000 INFO @ Mon, 29 Jun 2020 23:58:28: 29000000 INFO @ Mon, 29 Jun 2020 23:58:29: 30000000 INFO @ Mon, 29 Jun 2020 23:58:32: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:58:32: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:58:32: #1 total tags in treatment: 35563539 INFO @ Mon, 29 Jun 2020 23:58:32: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:58:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:58:33: #1 tags after filtering in treatment: 35563539 INFO @ Mon, 29 Jun 2020 23:58:33: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:58:33: #1 finished! INFO @ Mon, 29 Jun 2020 23:58:33: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:58:33: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:58:35: #2 number of paired peaks: 6 WARNING @ Mon, 29 Jun 2020 23:58:35: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:58:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981662/SRX3981662.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981662/SRX3981662.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981662/SRX3981662.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981662/SRX3981662.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:58:37: 30000000 INFO @ Mon, 29 Jun 2020 23:58:38: 31000000 INFO @ Mon, 29 Jun 2020 23:58:45: 31000000 INFO @ Mon, 29 Jun 2020 23:58:46: 32000000 INFO @ Mon, 29 Jun 2020 23:58:54: 32000000 INFO @ Mon, 29 Jun 2020 23:58:55: 33000000 INFO @ Mon, 29 Jun 2020 23:59:03: 33000000 INFO @ Mon, 29 Jun 2020 23:59:04: 34000000 INFO @ Mon, 29 Jun 2020 23:59:12: 34000000 INFO @ Mon, 29 Jun 2020 23:59:13: 35000000 INFO @ Mon, 29 Jun 2020 23:59:18: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:59:18: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:59:18: #1 total tags in treatment: 35563539 INFO @ Mon, 29 Jun 2020 23:59:18: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:59:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:59:19: #1 tags after filtering in treatment: 35563539 INFO @ Mon, 29 Jun 2020 23:59:19: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:59:19: #1 finished! INFO @ Mon, 29 Jun 2020 23:59:19: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:59:19: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:59:21: #2 number of paired peaks: 6 WARNING @ Mon, 29 Jun 2020 23:59:21: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:59:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981662/SRX3981662.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis INFO @ Mon, 29 Jun 2020 23:59:21: 35000000 needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981662/SRX3981662.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981662/SRX3981662.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981662/SRX3981662.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Mon, 29 Jun 2020 23:59:26: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:59:26: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:59:26: #1 total tags in treatment: 35563539 INFO @ Mon, 29 Jun 2020 23:59:26: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:59:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:59:26: #1 tags after filtering in treatment: 35563539 INFO @ Mon, 29 Jun 2020 23:59:26: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:59:26: #1 finished! INFO @ Mon, 29 Jun 2020 23:59:26: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:59:26: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:59:29: #2 number of paired peaks: 6 WARNING @ Mon, 29 Jun 2020 23:59:29: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:59:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981662/SRX3981662.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981662/SRX3981662.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981662/SRX3981662.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981662/SRX3981662.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling