Job ID = 6528043 SRX = SRX3981661 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:28:06 prefetch.2.10.7: 1) Downloading 'SRR7050367'... 2020-06-29T14:28:06 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:29:37 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:29:38 prefetch.2.10.7: 'SRR7050367' is valid 2020-06-29T14:29:38 prefetch.2.10.7: 1) 'SRR7050367' was downloaded successfully 2020-06-29T14:29:38 prefetch.2.10.7: 'SRR7050367' has 0 unresolved dependencies Read 15158190 spots for SRR7050367/SRR7050367.sra Written 15158190 spots for SRR7050367/SRR7050367.sra 2020-06-29T14:30:50 prefetch.2.10.7: 1) Downloading 'SRR7050368'... 2020-06-29T14:30:50 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:32:24 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:32:24 prefetch.2.10.7: 'SRR7050368' is valid 2020-06-29T14:32:24 prefetch.2.10.7: 1) 'SRR7050368' was downloaded successfully 2020-06-29T14:32:24 prefetch.2.10.7: 'SRR7050368' has 0 unresolved dependencies Read 15127886 spots for SRR7050368/SRR7050368.sra Written 15127886 spots for SRR7050368/SRR7050368.sra 2020-06-29T14:33:35 prefetch.2.10.7: 1) Downloading 'SRR7050369'... 2020-06-29T14:33:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:35:32 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:35:32 prefetch.2.10.7: 'SRR7050369' is valid 2020-06-29T14:35:32 prefetch.2.10.7: 1) 'SRR7050369' was downloaded successfully 2020-06-29T14:35:32 prefetch.2.10.7: 'SRR7050369' has 0 unresolved dependencies Read 15236838 spots for SRR7050369/SRR7050369.sra Written 15236838 spots for SRR7050369/SRR7050369.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:56 45522914 reads; of these: 45522914 (100.00%) were unpaired; of these: 1851486 (4.07%) aligned 0 times 32113702 (70.54%) aligned exactly 1 time 11557726 (25.39%) aligned >1 times 95.93% overall alignment rate Time searching: 00:14:56 Overall time: 00:14:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 5329520 / 43671428 = 0.1220 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:16:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981661/SRX3981661.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981661/SRX3981661.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981661/SRX3981661.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981661/SRX3981661.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:16:14: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:16:14: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:16:19: 1000000 INFO @ Tue, 30 Jun 2020 00:16:25: 2000000 INFO @ Tue, 30 Jun 2020 00:16:30: 3000000 INFO @ Tue, 30 Jun 2020 00:16:35: 4000000 INFO @ Tue, 30 Jun 2020 00:16:41: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:16:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981661/SRX3981661.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981661/SRX3981661.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981661/SRX3981661.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981661/SRX3981661.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:16:44: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:16:44: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:16:47: 6000000 INFO @ Tue, 30 Jun 2020 00:16:49: 1000000 INFO @ Tue, 30 Jun 2020 00:16:52: 7000000 INFO @ Tue, 30 Jun 2020 00:16:55: 2000000 INFO @ Tue, 30 Jun 2020 00:16:58: 8000000 INFO @ Tue, 30 Jun 2020 00:17:01: 3000000 INFO @ Tue, 30 Jun 2020 00:17:03: 9000000 INFO @ Tue, 30 Jun 2020 00:17:07: 4000000 INFO @ Tue, 30 Jun 2020 00:17:09: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:17:13: 5000000 INFO @ Tue, 30 Jun 2020 00:17:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981661/SRX3981661.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981661/SRX3981661.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981661/SRX3981661.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981661/SRX3981661.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:17:14: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:17:14: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:17:14: 11000000 INFO @ Tue, 30 Jun 2020 00:17:19: 6000000 INFO @ Tue, 30 Jun 2020 00:17:20: 1000000 INFO @ Tue, 30 Jun 2020 00:17:20: 12000000 INFO @ Tue, 30 Jun 2020 00:17:25: 7000000 INFO @ Tue, 30 Jun 2020 00:17:26: 2000000 INFO @ Tue, 30 Jun 2020 00:17:26: 13000000 INFO @ Tue, 30 Jun 2020 00:17:30: 8000000 INFO @ Tue, 30 Jun 2020 00:17:32: 3000000 INFO @ Tue, 30 Jun 2020 00:17:32: 14000000 INFO @ Tue, 30 Jun 2020 00:17:36: 9000000 INFO @ Tue, 30 Jun 2020 00:17:38: 4000000 INFO @ Tue, 30 Jun 2020 00:17:38: 15000000 INFO @ Tue, 30 Jun 2020 00:17:42: 10000000 INFO @ Tue, 30 Jun 2020 00:17:44: 5000000 INFO @ Tue, 30 Jun 2020 00:17:44: 16000000 INFO @ Tue, 30 Jun 2020 00:17:48: 11000000 INFO @ Tue, 30 Jun 2020 00:17:50: 6000000 INFO @ Tue, 30 Jun 2020 00:17:50: 17000000 INFO @ Tue, 30 Jun 2020 00:17:54: 12000000 INFO @ Tue, 30 Jun 2020 00:17:56: 7000000 INFO @ Tue, 30 Jun 2020 00:17:56: 18000000 INFO @ Tue, 30 Jun 2020 00:18:00: 13000000 INFO @ Tue, 30 Jun 2020 00:18:02: 8000000 INFO @ Tue, 30 Jun 2020 00:18:02: 19000000 INFO @ Tue, 30 Jun 2020 00:18:06: 14000000 INFO @ Tue, 30 Jun 2020 00:18:08: 20000000 INFO @ Tue, 30 Jun 2020 00:18:08: 9000000 INFO @ Tue, 30 Jun 2020 00:18:11: 15000000 INFO @ Tue, 30 Jun 2020 00:18:14: 21000000 INFO @ Tue, 30 Jun 2020 00:18:14: 10000000 INFO @ Tue, 30 Jun 2020 00:18:17: 16000000 INFO @ Tue, 30 Jun 2020 00:18:20: 22000000 INFO @ Tue, 30 Jun 2020 00:18:20: 11000000 INFO @ Tue, 30 Jun 2020 00:18:23: 17000000 INFO @ Tue, 30 Jun 2020 00:18:26: 23000000 INFO @ Tue, 30 Jun 2020 00:18:27: 12000000 INFO @ Tue, 30 Jun 2020 00:18:29: 18000000 INFO @ Tue, 30 Jun 2020 00:18:31: 24000000 INFO @ Tue, 30 Jun 2020 00:18:33: 13000000 INFO @ Tue, 30 Jun 2020 00:18:35: 19000000 INFO @ Tue, 30 Jun 2020 00:18:37: 25000000 INFO @ Tue, 30 Jun 2020 00:18:39: 14000000 INFO @ Tue, 30 Jun 2020 00:18:41: 20000000 INFO @ Tue, 30 Jun 2020 00:18:43: 26000000 INFO @ Tue, 30 Jun 2020 00:18:46: 15000000 INFO @ Tue, 30 Jun 2020 00:18:46: 21000000 INFO @ Tue, 30 Jun 2020 00:18:49: 27000000 INFO @ Tue, 30 Jun 2020 00:18:52: 16000000 INFO @ Tue, 30 Jun 2020 00:18:52: 22000000 INFO @ Tue, 30 Jun 2020 00:18:55: 28000000 INFO @ Tue, 30 Jun 2020 00:18:58: 17000000 INFO @ Tue, 30 Jun 2020 00:18:58: 23000000 INFO @ Tue, 30 Jun 2020 00:19:01: 29000000 INFO @ Tue, 30 Jun 2020 00:19:04: 18000000 INFO @ Tue, 30 Jun 2020 00:19:05: 24000000 INFO @ Tue, 30 Jun 2020 00:19:07: 30000000 INFO @ Tue, 30 Jun 2020 00:19:10: 19000000 INFO @ Tue, 30 Jun 2020 00:19:11: 25000000 INFO @ Tue, 30 Jun 2020 00:19:13: 31000000 INFO @ Tue, 30 Jun 2020 00:19:16: 20000000 INFO @ Tue, 30 Jun 2020 00:19:18: 26000000 INFO @ Tue, 30 Jun 2020 00:19:19: 32000000 INFO @ Tue, 30 Jun 2020 00:19:22: 21000000 INFO @ Tue, 30 Jun 2020 00:19:24: 27000000 INFO @ Tue, 30 Jun 2020 00:19:25: 33000000 INFO @ Tue, 30 Jun 2020 00:19:29: 22000000 INFO @ Tue, 30 Jun 2020 00:19:30: 28000000 INFO @ Tue, 30 Jun 2020 00:19:32: 34000000 INFO @ Tue, 30 Jun 2020 00:19:35: 23000000 INFO @ Tue, 30 Jun 2020 00:19:36: 29000000 INFO @ Tue, 30 Jun 2020 00:19:38: 35000000 INFO @ Tue, 30 Jun 2020 00:19:42: 24000000 INFO @ Tue, 30 Jun 2020 00:19:43: 30000000 INFO @ Tue, 30 Jun 2020 00:19:45: 36000000 INFO @ Tue, 30 Jun 2020 00:19:48: 25000000 INFO @ Tue, 30 Jun 2020 00:19:49: 31000000 INFO @ Tue, 30 Jun 2020 00:19:51: 37000000 INFO @ Tue, 30 Jun 2020 00:19:55: 26000000 INFO @ Tue, 30 Jun 2020 00:19:55: 32000000 INFO @ Tue, 30 Jun 2020 00:19:58: 38000000 INFO @ Tue, 30 Jun 2020 00:20:00: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:20:00: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:20:00: #1 total tags in treatment: 38341908 INFO @ Tue, 30 Jun 2020 00:20:00: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:20:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:20:01: #1 tags after filtering in treatment: 38341908 INFO @ Tue, 30 Jun 2020 00:20:01: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:20:01: #1 finished! INFO @ Tue, 30 Jun 2020 00:20:01: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:20:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:20:01: 27000000 INFO @ Tue, 30 Jun 2020 00:20:02: 33000000 INFO @ Tue, 30 Jun 2020 00:20:03: #2 number of paired peaks: 5 WARNING @ Tue, 30 Jun 2020 00:20:03: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:20:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981661/SRX3981661.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981661/SRX3981661.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981661/SRX3981661.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981661/SRX3981661.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:20:08: 28000000 INFO @ Tue, 30 Jun 2020 00:20:08: 34000000 INFO @ Tue, 30 Jun 2020 00:20:14: 35000000 INFO @ Tue, 30 Jun 2020 00:20:15: 29000000 INFO @ Tue, 30 Jun 2020 00:20:20: 36000000 INFO @ Tue, 30 Jun 2020 00:20:21: 30000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:20:26: 37000000 INFO @ Tue, 30 Jun 2020 00:20:28: 31000000 INFO @ Tue, 30 Jun 2020 00:20:32: 38000000 INFO @ Tue, 30 Jun 2020 00:20:34: 32000000 INFO @ Tue, 30 Jun 2020 00:20:34: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:20:34: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:20:34: #1 total tags in treatment: 38341908 INFO @ Tue, 30 Jun 2020 00:20:34: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:20:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:20:35: #1 tags after filtering in treatment: 38341908 INFO @ Tue, 30 Jun 2020 00:20:35: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:20:35: #1 finished! INFO @ Tue, 30 Jun 2020 00:20:35: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:20:35: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:20:37: #2 number of paired peaks: 5 WARNING @ Tue, 30 Jun 2020 00:20:37: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:20:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981661/SRX3981661.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981661/SRX3981661.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981661/SRX3981661.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981661/SRX3981661.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:20:40: 33000000 INFO @ Tue, 30 Jun 2020 00:20:47: 34000000 INFO @ Tue, 30 Jun 2020 00:20:53: 35000000 INFO @ Tue, 30 Jun 2020 00:20:59: 36000000 INFO @ Tue, 30 Jun 2020 00:21:05: 37000000 INFO @ Tue, 30 Jun 2020 00:21:10: 38000000 INFO @ Tue, 30 Jun 2020 00:21:12: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:21:12: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:21:12: #1 total tags in treatment: 38341908 INFO @ Tue, 30 Jun 2020 00:21:12: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:21:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:21:13: #1 tags after filtering in treatment: 38341908 INFO @ Tue, 30 Jun 2020 00:21:13: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:21:13: #1 finished! INFO @ Tue, 30 Jun 2020 00:21:13: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:21:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:21:16: #2 number of paired peaks: 5 WARNING @ Tue, 30 Jun 2020 00:21:16: Too few paired peaks (5) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:21:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981661/SRX3981661.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981661/SRX3981661.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981661/SRX3981661.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981661/SRX3981661.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。