Job ID = 6528042 SRX = SRX3981660 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:20:20 prefetch.2.10.7: 1) Downloading 'SRR7050364'... 2020-06-29T14:20:20 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:21:20 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:21:20 prefetch.2.10.7: 'SRR7050364' is valid 2020-06-29T14:21:20 prefetch.2.10.7: 1) 'SRR7050364' was downloaded successfully 2020-06-29T14:21:20 prefetch.2.10.7: 'SRR7050364' has 0 unresolved dependencies Read 13013478 spots for SRR7050364/SRR7050364.sra Written 13013478 spots for SRR7050364/SRR7050364.sra 2020-06-29T14:22:19 prefetch.2.10.7: 1) Downloading 'SRR7050365'... 2020-06-29T14:22:19 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:24:07 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:24:08 prefetch.2.10.7: 'SRR7050365' is valid 2020-06-29T14:24:08 prefetch.2.10.7: 1) 'SRR7050365' was downloaded successfully 2020-06-29T14:24:08 prefetch.2.10.7: 'SRR7050365' has 0 unresolved dependencies Read 12969060 spots for SRR7050365/SRR7050365.sra Written 12969060 spots for SRR7050365/SRR7050365.sra 2020-06-29T14:25:03 prefetch.2.10.7: 1) Downloading 'SRR7050366'... 2020-06-29T14:25:03 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:26:13 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:26:14 prefetch.2.10.7: 'SRR7050366' is valid 2020-06-29T14:26:14 prefetch.2.10.7: 1) 'SRR7050366' was downloaded successfully 2020-06-29T14:26:14 prefetch.2.10.7: 'SRR7050366' has 0 unresolved dependencies Read 13052562 spots for SRR7050366/SRR7050366.sra Written 13052562 spots for SRR7050366/SRR7050366.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:48 39035100 reads; of these: 39035100 (100.00%) were unpaired; of these: 1439373 (3.69%) aligned 0 times 27916052 (71.52%) aligned exactly 1 time 9679675 (24.80%) aligned >1 times 96.31% overall alignment rate Time searching: 00:11:48 Overall time: 00:11:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4053594 / 37595727 = 0.1078 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:56:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981660/SRX3981660.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981660/SRX3981660.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981660/SRX3981660.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981660/SRX3981660.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:56:14: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:56:14: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:56:20: 1000000 INFO @ Mon, 29 Jun 2020 23:56:25: 2000000 INFO @ Mon, 29 Jun 2020 23:56:31: 3000000 INFO @ Mon, 29 Jun 2020 23:56:36: 4000000 INFO @ Mon, 29 Jun 2020 23:56:41: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:56:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981660/SRX3981660.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981660/SRX3981660.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981660/SRX3981660.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981660/SRX3981660.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:56:44: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:56:44: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:56:47: 6000000 INFO @ Mon, 29 Jun 2020 23:56:50: 1000000 INFO @ Mon, 29 Jun 2020 23:56:53: 7000000 INFO @ Mon, 29 Jun 2020 23:56:56: 2000000 INFO @ Mon, 29 Jun 2020 23:56:59: 8000000 INFO @ Mon, 29 Jun 2020 23:57:02: 3000000 INFO @ Mon, 29 Jun 2020 23:57:05: 9000000 INFO @ Mon, 29 Jun 2020 23:57:08: 4000000 INFO @ Mon, 29 Jun 2020 23:57:10: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:57:14: 5000000 INFO @ Mon, 29 Jun 2020 23:57:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981660/SRX3981660.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981660/SRX3981660.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981660/SRX3981660.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981660/SRX3981660.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:57:14: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:57:14: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:57:16: 11000000 INFO @ Mon, 29 Jun 2020 23:57:20: 6000000 INFO @ Mon, 29 Jun 2020 23:57:21: 1000000 INFO @ Mon, 29 Jun 2020 23:57:23: 12000000 INFO @ Mon, 29 Jun 2020 23:57:26: 7000000 INFO @ Mon, 29 Jun 2020 23:57:29: 2000000 INFO @ Mon, 29 Jun 2020 23:57:29: 13000000 INFO @ Mon, 29 Jun 2020 23:57:33: 8000000 INFO @ Mon, 29 Jun 2020 23:57:35: 14000000 INFO @ Mon, 29 Jun 2020 23:57:36: 3000000 INFO @ Mon, 29 Jun 2020 23:57:39: 9000000 INFO @ Mon, 29 Jun 2020 23:57:42: 15000000 INFO @ Mon, 29 Jun 2020 23:57:43: 4000000 INFO @ Mon, 29 Jun 2020 23:57:45: 10000000 INFO @ Mon, 29 Jun 2020 23:57:48: 16000000 INFO @ Mon, 29 Jun 2020 23:57:50: 5000000 INFO @ Mon, 29 Jun 2020 23:57:51: 11000000 INFO @ Mon, 29 Jun 2020 23:57:54: 17000000 INFO @ Mon, 29 Jun 2020 23:57:57: 6000000 INFO @ Mon, 29 Jun 2020 23:57:57: 12000000 INFO @ Mon, 29 Jun 2020 23:58:00: 18000000 INFO @ Mon, 29 Jun 2020 23:58:03: 13000000 INFO @ Mon, 29 Jun 2020 23:58:04: 7000000 INFO @ Mon, 29 Jun 2020 23:58:07: 19000000 INFO @ Mon, 29 Jun 2020 23:58:10: 14000000 INFO @ Mon, 29 Jun 2020 23:58:10: 8000000 INFO @ Mon, 29 Jun 2020 23:58:13: 20000000 INFO @ Mon, 29 Jun 2020 23:58:16: 15000000 INFO @ Mon, 29 Jun 2020 23:58:17: 9000000 INFO @ Mon, 29 Jun 2020 23:58:20: 21000000 INFO @ Mon, 29 Jun 2020 23:58:23: 16000000 INFO @ Mon, 29 Jun 2020 23:58:24: 10000000 INFO @ Mon, 29 Jun 2020 23:58:26: 22000000 INFO @ Mon, 29 Jun 2020 23:58:29: 17000000 INFO @ Mon, 29 Jun 2020 23:58:31: 11000000 INFO @ Mon, 29 Jun 2020 23:58:32: 23000000 INFO @ Mon, 29 Jun 2020 23:58:36: 18000000 INFO @ Mon, 29 Jun 2020 23:58:38: 12000000 INFO @ Mon, 29 Jun 2020 23:58:39: 24000000 INFO @ Mon, 29 Jun 2020 23:58:42: 19000000 INFO @ Mon, 29 Jun 2020 23:58:45: 13000000 INFO @ Mon, 29 Jun 2020 23:58:46: 25000000 INFO @ Mon, 29 Jun 2020 23:58:49: 20000000 INFO @ Mon, 29 Jun 2020 23:58:52: 14000000 INFO @ Mon, 29 Jun 2020 23:58:52: 26000000 INFO @ Mon, 29 Jun 2020 23:58:55: 21000000 INFO @ Mon, 29 Jun 2020 23:58:59: 27000000 INFO @ Mon, 29 Jun 2020 23:58:59: 15000000 INFO @ Mon, 29 Jun 2020 23:59:02: 22000000 INFO @ Mon, 29 Jun 2020 23:59:05: 28000000 INFO @ Mon, 29 Jun 2020 23:59:06: 16000000 INFO @ Mon, 29 Jun 2020 23:59:08: 23000000 INFO @ Mon, 29 Jun 2020 23:59:12: 29000000 INFO @ Mon, 29 Jun 2020 23:59:14: 17000000 INFO @ Mon, 29 Jun 2020 23:59:15: 24000000 INFO @ Mon, 29 Jun 2020 23:59:19: 30000000 INFO @ Mon, 29 Jun 2020 23:59:21: 18000000 INFO @ Mon, 29 Jun 2020 23:59:21: 25000000 INFO @ Mon, 29 Jun 2020 23:59:25: 31000000 INFO @ Mon, 29 Jun 2020 23:59:28: 19000000 INFO @ Mon, 29 Jun 2020 23:59:28: 26000000 INFO @ Mon, 29 Jun 2020 23:59:32: 32000000 INFO @ Mon, 29 Jun 2020 23:59:35: 27000000 INFO @ Mon, 29 Jun 2020 23:59:35: 20000000 INFO @ Mon, 29 Jun 2020 23:59:39: 33000000 INFO @ Mon, 29 Jun 2020 23:59:41: 28000000 INFO @ Mon, 29 Jun 2020 23:59:42: 21000000 INFO @ Mon, 29 Jun 2020 23:59:42: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:59:42: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:59:42: #1 total tags in treatment: 33542133 INFO @ Mon, 29 Jun 2020 23:59:42: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:59:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:59:43: #1 tags after filtering in treatment: 33542133 INFO @ Mon, 29 Jun 2020 23:59:43: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:59:43: #1 finished! INFO @ Mon, 29 Jun 2020 23:59:43: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:59:43: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:59:45: #2 number of paired peaks: 10 WARNING @ Mon, 29 Jun 2020 23:59:45: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:59:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981660/SRX3981660.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981660/SRX3981660.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981660/SRX3981660.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981660/SRX3981660.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:59:48: 29000000 INFO @ Mon, 29 Jun 2020 23:59:49: 22000000 INFO @ Mon, 29 Jun 2020 23:59:54: 30000000 INFO @ Mon, 29 Jun 2020 23:59:56: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:00:01: 31000000 INFO @ Tue, 30 Jun 2020 00:00:03: 24000000 INFO @ Tue, 30 Jun 2020 00:00:08: 32000000 INFO @ Tue, 30 Jun 2020 00:00:11: 25000000 INFO @ Tue, 30 Jun 2020 00:00:15: 33000000 INFO @ Tue, 30 Jun 2020 00:00:18: 26000000 INFO @ Tue, 30 Jun 2020 00:00:19: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:00:19: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:00:19: #1 total tags in treatment: 33542133 INFO @ Tue, 30 Jun 2020 00:00:19: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:00:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:00:19: #1 tags after filtering in treatment: 33542133 INFO @ Tue, 30 Jun 2020 00:00:19: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:00:19: #1 finished! INFO @ Tue, 30 Jun 2020 00:00:19: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:00:19: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:00:22: #2 number of paired peaks: 10 WARNING @ Tue, 30 Jun 2020 00:00:22: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:00:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981660/SRX3981660.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981660/SRX3981660.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981660/SRX3981660.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981660/SRX3981660.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:00:25: 27000000 INFO @ Tue, 30 Jun 2020 00:00:32: 28000000 INFO @ Tue, 30 Jun 2020 00:00:38: 29000000 INFO @ Tue, 30 Jun 2020 00:00:45: 30000000 INFO @ Tue, 30 Jun 2020 00:00:52: 31000000 INFO @ Tue, 30 Jun 2020 00:00:59: 32000000 INFO @ Tue, 30 Jun 2020 00:01:06: 33000000 INFO @ Tue, 30 Jun 2020 00:01:09: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:01:09: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:01:09: #1 total tags in treatment: 33542133 INFO @ Tue, 30 Jun 2020 00:01:09: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:01:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:01:10: #1 tags after filtering in treatment: 33542133 INFO @ Tue, 30 Jun 2020 00:01:10: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:01:10: #1 finished! INFO @ Tue, 30 Jun 2020 00:01:10: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:01:10: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:01:12: #2 number of paired peaks: 10 WARNING @ Tue, 30 Jun 2020 00:01:12: Too few paired peaks (10) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:01:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981660/SRX3981660.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981660/SRX3981660.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981660/SRX3981660.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981660/SRX3981660.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。