Job ID = 6528041 SRX = SRX3981659 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:16:05 prefetch.2.10.7: 1) Downloading 'SRR7050361'... 2020-06-29T14:16:05 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:17:08 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:17:08 prefetch.2.10.7: 'SRR7050361' is valid 2020-06-29T14:17:08 prefetch.2.10.7: 1) 'SRR7050361' was downloaded successfully 2020-06-29T14:17:08 prefetch.2.10.7: 'SRR7050361' has 0 unresolved dependencies Read 13100638 spots for SRR7050361/SRR7050361.sra Written 13100638 spots for SRR7050361/SRR7050361.sra 2020-06-29T14:18:04 prefetch.2.10.7: 1) Downloading 'SRR7050362'... 2020-06-29T14:18:04 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:19:00 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:19:01 prefetch.2.10.7: 'SRR7050362' is valid 2020-06-29T14:19:01 prefetch.2.10.7: 1) 'SRR7050362' was downloaded successfully 2020-06-29T14:19:01 prefetch.2.10.7: 'SRR7050362' has 0 unresolved dependencies Read 13066398 spots for SRR7050362/SRR7050362.sra Written 13066398 spots for SRR7050362/SRR7050362.sra 2020-06-29T14:19:58 prefetch.2.10.7: 1) Downloading 'SRR7050363'... 2020-06-29T14:19:58 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:21:14 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:21:15 prefetch.2.10.7: 'SRR7050363' is valid 2020-06-29T14:21:15 prefetch.2.10.7: 1) 'SRR7050363' was downloaded successfully 2020-06-29T14:21:15 prefetch.2.10.7: 'SRR7050363' has 0 unresolved dependencies Read 13152249 spots for SRR7050363/SRR7050363.sra Written 13152249 spots for SRR7050363/SRR7050363.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:33 39319285 reads; of these: 39319285 (100.00%) were unpaired; of these: 1271682 (3.23%) aligned 0 times 27876248 (70.90%) aligned exactly 1 time 10171355 (25.87%) aligned >1 times 96.77% overall alignment rate Time searching: 00:12:33 Overall time: 00:12:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4377108 / 38047603 = 0.1150 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:52:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981659/SRX3981659.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981659/SRX3981659.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981659/SRX3981659.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981659/SRX3981659.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:52:31: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:52:31: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:52:37: 1000000 INFO @ Mon, 29 Jun 2020 23:52:43: 2000000 INFO @ Mon, 29 Jun 2020 23:52:48: 3000000 INFO @ Mon, 29 Jun 2020 23:52:54: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:52:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981659/SRX3981659.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981659/SRX3981659.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981659/SRX3981659.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981659/SRX3981659.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:52:59: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:52:59: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:52:59: 5000000 INFO @ Mon, 29 Jun 2020 23:53:05: 1000000 INFO @ Mon, 29 Jun 2020 23:53:05: 6000000 INFO @ Mon, 29 Jun 2020 23:53:11: 2000000 INFO @ Mon, 29 Jun 2020 23:53:12: 7000000 INFO @ Mon, 29 Jun 2020 23:53:17: 3000000 INFO @ Mon, 29 Jun 2020 23:53:17: 8000000 INFO @ Mon, 29 Jun 2020 23:53:23: 4000000 INFO @ Mon, 29 Jun 2020 23:53:23: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:53:29: 5000000 INFO @ Mon, 29 Jun 2020 23:53:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981659/SRX3981659.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981659/SRX3981659.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981659/SRX3981659.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981659/SRX3981659.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:53:29: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:53:29: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:53:29: 10000000 INFO @ Mon, 29 Jun 2020 23:53:35: 6000000 INFO @ Mon, 29 Jun 2020 23:53:35: 1000000 INFO @ Mon, 29 Jun 2020 23:53:35: 11000000 INFO @ Mon, 29 Jun 2020 23:53:41: 7000000 INFO @ Mon, 29 Jun 2020 23:53:41: 12000000 INFO @ Mon, 29 Jun 2020 23:53:41: 2000000 INFO @ Mon, 29 Jun 2020 23:53:47: 8000000 INFO @ Mon, 29 Jun 2020 23:53:47: 13000000 INFO @ Mon, 29 Jun 2020 23:53:48: 3000000 INFO @ Mon, 29 Jun 2020 23:53:53: 9000000 INFO @ Mon, 29 Jun 2020 23:53:53: 14000000 INFO @ Mon, 29 Jun 2020 23:53:54: 4000000 INFO @ Mon, 29 Jun 2020 23:53:59: 10000000 INFO @ Mon, 29 Jun 2020 23:53:59: 15000000 INFO @ Mon, 29 Jun 2020 23:54:00: 5000000 INFO @ Mon, 29 Jun 2020 23:54:05: 11000000 INFO @ Mon, 29 Jun 2020 23:54:05: 16000000 INFO @ Mon, 29 Jun 2020 23:54:06: 6000000 INFO @ Mon, 29 Jun 2020 23:54:11: 12000000 INFO @ Mon, 29 Jun 2020 23:54:11: 17000000 INFO @ Mon, 29 Jun 2020 23:54:12: 7000000 INFO @ Mon, 29 Jun 2020 23:54:17: 13000000 INFO @ Mon, 29 Jun 2020 23:54:18: 18000000 INFO @ Mon, 29 Jun 2020 23:54:18: 8000000 INFO @ Mon, 29 Jun 2020 23:54:23: 14000000 INFO @ Mon, 29 Jun 2020 23:54:24: 19000000 INFO @ Mon, 29 Jun 2020 23:54:24: 9000000 INFO @ Mon, 29 Jun 2020 23:54:29: 15000000 INFO @ Mon, 29 Jun 2020 23:54:30: 20000000 INFO @ Mon, 29 Jun 2020 23:54:31: 10000000 INFO @ Mon, 29 Jun 2020 23:54:35: 16000000 INFO @ Mon, 29 Jun 2020 23:54:37: 21000000 INFO @ Mon, 29 Jun 2020 23:54:37: 11000000 INFO @ Mon, 29 Jun 2020 23:54:42: 17000000 INFO @ Mon, 29 Jun 2020 23:54:43: 22000000 INFO @ Mon, 29 Jun 2020 23:54:43: 12000000 INFO @ Mon, 29 Jun 2020 23:54:48: 18000000 INFO @ Mon, 29 Jun 2020 23:54:50: 13000000 INFO @ Mon, 29 Jun 2020 23:54:50: 23000000 INFO @ Mon, 29 Jun 2020 23:54:55: 19000000 INFO @ Mon, 29 Jun 2020 23:54:56: 14000000 INFO @ Mon, 29 Jun 2020 23:54:57: 24000000 INFO @ Mon, 29 Jun 2020 23:55:01: 20000000 INFO @ Mon, 29 Jun 2020 23:55:03: 15000000 INFO @ Mon, 29 Jun 2020 23:55:03: 25000000 INFO @ Mon, 29 Jun 2020 23:55:08: 21000000 INFO @ Mon, 29 Jun 2020 23:55:09: 16000000 INFO @ Mon, 29 Jun 2020 23:55:10: 26000000 INFO @ Mon, 29 Jun 2020 23:55:14: 22000000 INFO @ Mon, 29 Jun 2020 23:55:16: 17000000 INFO @ Mon, 29 Jun 2020 23:55:17: 27000000 INFO @ Mon, 29 Jun 2020 23:55:20: 23000000 INFO @ Mon, 29 Jun 2020 23:55:22: 18000000 INFO @ Mon, 29 Jun 2020 23:55:23: 28000000 INFO @ Mon, 29 Jun 2020 23:55:27: 24000000 INFO @ Mon, 29 Jun 2020 23:55:28: 19000000 INFO @ Mon, 29 Jun 2020 23:55:29: 29000000 INFO @ Mon, 29 Jun 2020 23:55:33: 25000000 INFO @ Mon, 29 Jun 2020 23:55:34: 20000000 INFO @ Mon, 29 Jun 2020 23:55:36: 30000000 INFO @ Mon, 29 Jun 2020 23:55:39: 26000000 INFO @ Mon, 29 Jun 2020 23:55:41: 21000000 INFO @ Mon, 29 Jun 2020 23:55:42: 31000000 INFO @ Mon, 29 Jun 2020 23:55:46: 27000000 INFO @ Mon, 29 Jun 2020 23:55:47: 22000000 INFO @ Mon, 29 Jun 2020 23:55:49: 32000000 INFO @ Mon, 29 Jun 2020 23:55:52: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:55:53: 23000000 INFO @ Mon, 29 Jun 2020 23:55:55: 33000000 INFO @ Mon, 29 Jun 2020 23:55:59: 29000000 INFO @ Mon, 29 Jun 2020 23:55:59: 24000000 INFO @ Mon, 29 Jun 2020 23:56:00: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:56:00: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:56:00: #1 total tags in treatment: 33670495 INFO @ Mon, 29 Jun 2020 23:56:00: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:56:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:56:00: #1 tags after filtering in treatment: 33670495 INFO @ Mon, 29 Jun 2020 23:56:00: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:56:00: #1 finished! INFO @ Mon, 29 Jun 2020 23:56:00: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:56:00: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:56:02: #2 number of paired peaks: 13 WARNING @ Mon, 29 Jun 2020 23:56:02: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:56:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981659/SRX3981659.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981659/SRX3981659.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981659/SRX3981659.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981659/SRX3981659.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:56:05: 30000000 INFO @ Mon, 29 Jun 2020 23:56:06: 25000000 INFO @ Mon, 29 Jun 2020 23:56:11: 31000000 INFO @ Mon, 29 Jun 2020 23:56:12: 26000000 INFO @ Mon, 29 Jun 2020 23:56:17: 32000000 INFO @ Mon, 29 Jun 2020 23:56:18: 27000000 INFO @ Mon, 29 Jun 2020 23:56:23: 33000000 INFO @ Mon, 29 Jun 2020 23:56:24: 28000000 INFO @ Mon, 29 Jun 2020 23:56:28: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:56:28: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:56:28: #1 total tags in treatment: 33670495 INFO @ Mon, 29 Jun 2020 23:56:28: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:56:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:56:28: #1 tags after filtering in treatment: 33670495 INFO @ Mon, 29 Jun 2020 23:56:28: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:56:28: #1 finished! INFO @ Mon, 29 Jun 2020 23:56:28: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:56:28: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:56:30: #2 number of paired peaks: 13 WARNING @ Mon, 29 Jun 2020 23:56:30: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:56:30: Process for pairing-model is terminated! INFO @ Mon, 29 Jun 2020 23:56:30: 29000000 cut: /home/okishinya/chipatlas/results/dm3/SRX3981659/SRX3981659.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981659/SRX3981659.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981659/SRX3981659.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981659/SRX3981659.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:56:36: 30000000 INFO @ Mon, 29 Jun 2020 23:56:42: 31000000 INFO @ Mon, 29 Jun 2020 23:56:48: 32000000 INFO @ Mon, 29 Jun 2020 23:56:53: 33000000 INFO @ Mon, 29 Jun 2020 23:56:57: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:56:57: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:56:57: #1 total tags in treatment: 33670495 INFO @ Mon, 29 Jun 2020 23:56:57: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:56:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:56:58: #1 tags after filtering in treatment: 33670495 INFO @ Mon, 29 Jun 2020 23:56:58: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:56:58: #1 finished! INFO @ Mon, 29 Jun 2020 23:56:58: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:56:58: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:57:00: #2 number of paired peaks: 13 WARNING @ Mon, 29 Jun 2020 23:57:00: Too few paired peaks (13) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:57:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981659/SRX3981659.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981659/SRX3981659.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981659/SRX3981659.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981659/SRX3981659.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。