Job ID = 2590423 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 21,673,209 reads read : 43,346,418 reads written : 21,673,209 reads 0-length : 21,673,209 spots read : 21,638,169 reads read : 43,276,338 reads written : 21,638,169 reads 0-length : 21,638,169 spots read : 21,810,801 reads read : 43,621,602 reads written : 21,810,801 reads 0-length : 21,810,801 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:25:29 65122179 reads; of these: 65122179 (100.00%) were unpaired; of these: 1839114 (2.82%) aligned 0 times 46083132 (70.76%) aligned exactly 1 time 17199933 (26.41%) aligned >1 times 97.18% overall alignment rate Time searching: 00:25:29 Overall time: 00:25:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 10337517 / 63283065 = 0.1634 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 22:10:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981658/SRX3981658.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981658/SRX3981658.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981658/SRX3981658.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981658/SRX3981658.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:10:32: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:10:32: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:10:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981658/SRX3981658.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981658/SRX3981658.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981658/SRX3981658.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981658/SRX3981658.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:10:33: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:10:33: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:10:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981658/SRX3981658.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981658/SRX3981658.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981658/SRX3981658.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981658/SRX3981658.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:10:34: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:10:34: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:10:41: 1000000 INFO @ Mon, 12 Aug 2019 22:10:42: 1000000 INFO @ Mon, 12 Aug 2019 22:10:44: 1000000 INFO @ Mon, 12 Aug 2019 22:10:48: 2000000 INFO @ Mon, 12 Aug 2019 22:10:51: 2000000 INFO @ Mon, 12 Aug 2019 22:10:54: 2000000 INFO @ Mon, 12 Aug 2019 22:10:57: 3000000 INFO @ Mon, 12 Aug 2019 22:11:01: 3000000 INFO @ Mon, 12 Aug 2019 22:11:04: 3000000 INFO @ Mon, 12 Aug 2019 22:11:05: 4000000 INFO @ Mon, 12 Aug 2019 22:11:11: 4000000 INFO @ Mon, 12 Aug 2019 22:11:14: 5000000 INFO @ Mon, 12 Aug 2019 22:11:14: 4000000 INFO @ Mon, 12 Aug 2019 22:11:21: 5000000 INFO @ Mon, 12 Aug 2019 22:11:22: 6000000 INFO @ Mon, 12 Aug 2019 22:11:24: 5000000 INFO @ Mon, 12 Aug 2019 22:11:30: 6000000 INFO @ Mon, 12 Aug 2019 22:11:31: 7000000 INFO @ Mon, 12 Aug 2019 22:11:33: 6000000 INFO @ Mon, 12 Aug 2019 22:11:39: 8000000 INFO @ Mon, 12 Aug 2019 22:11:40: 7000000 INFO @ Mon, 12 Aug 2019 22:11:43: 7000000 INFO @ Mon, 12 Aug 2019 22:11:47: 9000000 INFO @ Mon, 12 Aug 2019 22:11:50: 8000000 INFO @ Mon, 12 Aug 2019 22:11:53: 8000000 INFO @ Mon, 12 Aug 2019 22:11:55: 10000000 INFO @ Mon, 12 Aug 2019 22:11:59: 9000000 INFO @ Mon, 12 Aug 2019 22:12:03: 9000000 INFO @ Mon, 12 Aug 2019 22:12:03: 11000000 INFO @ Mon, 12 Aug 2019 22:12:09: 10000000 INFO @ Mon, 12 Aug 2019 22:12:12: 10000000 INFO @ Mon, 12 Aug 2019 22:12:12: 12000000 INFO @ Mon, 12 Aug 2019 22:12:18: 11000000 INFO @ Mon, 12 Aug 2019 22:12:21: 13000000 INFO @ Mon, 12 Aug 2019 22:12:21: 11000000 INFO @ Mon, 12 Aug 2019 22:12:29: 12000000 INFO @ Mon, 12 Aug 2019 22:12:30: 14000000 INFO @ Mon, 12 Aug 2019 22:12:31: 12000000 INFO @ Mon, 12 Aug 2019 22:12:38: 13000000 INFO @ Mon, 12 Aug 2019 22:12:40: 13000000 INFO @ Mon, 12 Aug 2019 22:12:41: 15000000 INFO @ Mon, 12 Aug 2019 22:12:48: 14000000 INFO @ Mon, 12 Aug 2019 22:12:49: 14000000 INFO @ Mon, 12 Aug 2019 22:12:52: 16000000 INFO @ Mon, 12 Aug 2019 22:12:59: 15000000 INFO @ Mon, 12 Aug 2019 22:12:59: 15000000 INFO @ Mon, 12 Aug 2019 22:13:02: 17000000 INFO @ Mon, 12 Aug 2019 22:13:09: 16000000 INFO @ Mon, 12 Aug 2019 22:13:10: 16000000 INFO @ Mon, 12 Aug 2019 22:13:12: 18000000 INFO @ Mon, 12 Aug 2019 22:13:19: 17000000 INFO @ Mon, 12 Aug 2019 22:13:20: 17000000 INFO @ Mon, 12 Aug 2019 22:13:21: 19000000 INFO @ Mon, 12 Aug 2019 22:13:28: 18000000 INFO @ Mon, 12 Aug 2019 22:13:29: 20000000 INFO @ Mon, 12 Aug 2019 22:13:30: 18000000 INFO @ Mon, 12 Aug 2019 22:13:37: 21000000 INFO @ Mon, 12 Aug 2019 22:13:38: 19000000 INFO @ Mon, 12 Aug 2019 22:13:39: 19000000 INFO @ Mon, 12 Aug 2019 22:13:45: 22000000 INFO @ Mon, 12 Aug 2019 22:13:47: 20000000 INFO @ Mon, 12 Aug 2019 22:13:48: 20000000 INFO @ Mon, 12 Aug 2019 22:13:53: 23000000 INFO @ Mon, 12 Aug 2019 22:13:57: 21000000 INFO @ Mon, 12 Aug 2019 22:13:58: 21000000 INFO @ Mon, 12 Aug 2019 22:14:01: 24000000 INFO @ Mon, 12 Aug 2019 22:14:06: 22000000 INFO @ Mon, 12 Aug 2019 22:14:07: 22000000 INFO @ Mon, 12 Aug 2019 22:14:09: 25000000 INFO @ Mon, 12 Aug 2019 22:14:14: 23000000 INFO @ Mon, 12 Aug 2019 22:14:15: 26000000 INFO @ Mon, 12 Aug 2019 22:14:15: 23000000 INFO @ Mon, 12 Aug 2019 22:14:22: 27000000 INFO @ Mon, 12 Aug 2019 22:14:23: 24000000 INFO @ Mon, 12 Aug 2019 22:14:24: 24000000 INFO @ Mon, 12 Aug 2019 22:14:28: 28000000 INFO @ Mon, 12 Aug 2019 22:14:31: 25000000 INFO @ Mon, 12 Aug 2019 22:14:32: 25000000 INFO @ Mon, 12 Aug 2019 22:14:35: 29000000 INFO @ Mon, 12 Aug 2019 22:14:38: 26000000 INFO @ Mon, 12 Aug 2019 22:14:39: 26000000 INFO @ Mon, 12 Aug 2019 22:14:42: 30000000 INFO @ Mon, 12 Aug 2019 22:14:46: 27000000 INFO @ Mon, 12 Aug 2019 22:14:46: 27000000 INFO @ Mon, 12 Aug 2019 22:14:48: 31000000 INFO @ Mon, 12 Aug 2019 22:14:53: 28000000 INFO @ Mon, 12 Aug 2019 22:14:54: 28000000 INFO @ Mon, 12 Aug 2019 22:14:55: 32000000 INFO @ Mon, 12 Aug 2019 22:15:00: 29000000 INFO @ Mon, 12 Aug 2019 22:15:01: 33000000 INFO @ Mon, 12 Aug 2019 22:15:01: 29000000 INFO @ Mon, 12 Aug 2019 22:15:08: 34000000 INFO @ Mon, 12 Aug 2019 22:15:08: 30000000 INFO @ Mon, 12 Aug 2019 22:15:08: 30000000 INFO @ Mon, 12 Aug 2019 22:15:14: 35000000 INFO @ Mon, 12 Aug 2019 22:15:15: 31000000 INFO @ Mon, 12 Aug 2019 22:15:16: 31000000 INFO @ Mon, 12 Aug 2019 22:15:21: 36000000 INFO @ Mon, 12 Aug 2019 22:15:22: 32000000 INFO @ Mon, 12 Aug 2019 22:15:23: 32000000 INFO @ Mon, 12 Aug 2019 22:15:28: 37000000 INFO @ Mon, 12 Aug 2019 22:15:29: 33000000 INFO @ Mon, 12 Aug 2019 22:15:30: 33000000 INFO @ Mon, 12 Aug 2019 22:15:35: 38000000 INFO @ Mon, 12 Aug 2019 22:15:36: 34000000 INFO @ Mon, 12 Aug 2019 22:15:37: 34000000 INFO @ Mon, 12 Aug 2019 22:15:43: 35000000 INFO @ Mon, 12 Aug 2019 22:15:43: 39000000 INFO @ Mon, 12 Aug 2019 22:15:45: 35000000 INFO @ Mon, 12 Aug 2019 22:15:49: 36000000 INFO @ Mon, 12 Aug 2019 22:15:50: 40000000 INFO @ Mon, 12 Aug 2019 22:15:52: 36000000 INFO @ Mon, 12 Aug 2019 22:15:56: 37000000 INFO @ Mon, 12 Aug 2019 22:15:58: 41000000 INFO @ Mon, 12 Aug 2019 22:15:59: 37000000 INFO @ Mon, 12 Aug 2019 22:16:03: 38000000 INFO @ Mon, 12 Aug 2019 22:16:05: 42000000 INFO @ Mon, 12 Aug 2019 22:16:07: 38000000 INFO @ Mon, 12 Aug 2019 22:16:09: 39000000 INFO @ Mon, 12 Aug 2019 22:16:12: 43000000 INFO @ Mon, 12 Aug 2019 22:16:14: 39000000 INFO @ Mon, 12 Aug 2019 22:16:16: 40000000 INFO @ Mon, 12 Aug 2019 22:16:19: 44000000 INFO @ Mon, 12 Aug 2019 22:16:21: 40000000 INFO @ Mon, 12 Aug 2019 22:16:23: 41000000 INFO @ Mon, 12 Aug 2019 22:16:26: 45000000 INFO @ Mon, 12 Aug 2019 22:16:29: 41000000 INFO @ Mon, 12 Aug 2019 22:16:30: 42000000 INFO @ Mon, 12 Aug 2019 22:16:33: 46000000 INFO @ Mon, 12 Aug 2019 22:16:36: 42000000 INFO @ Mon, 12 Aug 2019 22:16:37: 43000000 INFO @ Mon, 12 Aug 2019 22:16:41: 47000000 INFO @ Mon, 12 Aug 2019 22:16:44: 44000000 INFO @ Mon, 12 Aug 2019 22:16:44: 43000000 INFO @ Mon, 12 Aug 2019 22:16:48: 48000000 INFO @ Mon, 12 Aug 2019 22:16:50: 45000000 INFO @ Mon, 12 Aug 2019 22:16:51: 44000000 INFO @ Mon, 12 Aug 2019 22:16:55: 49000000 INFO @ Mon, 12 Aug 2019 22:16:57: 46000000 INFO @ Mon, 12 Aug 2019 22:16:58: 45000000 INFO @ Mon, 12 Aug 2019 22:17:02: 50000000 INFO @ Mon, 12 Aug 2019 22:17:03: 47000000 INFO @ Mon, 12 Aug 2019 22:17:06: 46000000 INFO @ Mon, 12 Aug 2019 22:17:09: 51000000 INFO @ Mon, 12 Aug 2019 22:17:10: 48000000 INFO @ Mon, 12 Aug 2019 22:17:13: 47000000 INFO @ Mon, 12 Aug 2019 22:17:16: 52000000 INFO @ Mon, 12 Aug 2019 22:17:17: 49000000 INFO @ Mon, 12 Aug 2019 22:17:20: 48000000 INFO @ Mon, 12 Aug 2019 22:17:23: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:17:23: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:17:23: #1 total tags in treatment: 52945548 INFO @ Mon, 12 Aug 2019 22:17:23: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:17:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:17:23: 50000000 INFO @ Mon, 12 Aug 2019 22:17:24: #1 tags after filtering in treatment: 52945548 INFO @ Mon, 12 Aug 2019 22:17:24: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:17:24: #1 finished! INFO @ Mon, 12 Aug 2019 22:17:24: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:17:24: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:17:27: 49000000 INFO @ Mon, 12 Aug 2019 22:17:29: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 22:17:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:17:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981658/SRX3981658.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981658/SRX3981658.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981658/SRX3981658.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981658/SRX3981658.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 22:17:30: 51000000 INFO @ Mon, 12 Aug 2019 22:17:34: 50000000 INFO @ Mon, 12 Aug 2019 22:17:37: 52000000 INFO @ Mon, 12 Aug 2019 22:17:41: 51000000 INFO @ Mon, 12 Aug 2019 22:17:45: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:17:45: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:17:45: #1 total tags in treatment: 52945548 INFO @ Mon, 12 Aug 2019 22:17:45: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:17:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:17:46: #1 tags after filtering in treatment: 52945548 INFO @ Mon, 12 Aug 2019 22:17:46: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:17:46: #1 finished! INFO @ Mon, 12 Aug 2019 22:17:46: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:17:46: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:17:48: 52000000 INFO @ Mon, 12 Aug 2019 22:17:50: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 22:17:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:17:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981658/SRX3981658.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981658/SRX3981658.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981658/SRX3981658.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981658/SRX3981658.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 22:17:54: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 22:17:54: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 22:17:54: #1 total tags in treatment: 52945548 INFO @ Mon, 12 Aug 2019 22:17:54: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:17:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:17:55: #1 tags after filtering in treatment: 52945548 INFO @ Mon, 12 Aug 2019 22:17:55: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:17:55: #1 finished! INFO @ Mon, 12 Aug 2019 22:17:55: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:17:55: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:18:00: #2 number of paired peaks: 0 WARNING @ Mon, 12 Aug 2019 22:18:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:18:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981658/SRX3981658.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981658/SRX3981658.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981658/SRX3981658.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981658/SRX3981658.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。