Job ID = 6528040 SRX = SRX3981657 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:15:47 prefetch.2.10.7: 1) Downloading 'SRR7050355'... 2020-06-29T14:15:47 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:16:45 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:16:46 prefetch.2.10.7: 'SRR7050355' is valid 2020-06-29T14:16:46 prefetch.2.10.7: 1) 'SRR7050355' was downloaded successfully 2020-06-29T14:16:46 prefetch.2.10.7: 'SRR7050355' has 0 unresolved dependencies Read 11634168 spots for SRR7050355/SRR7050355.sra Written 11634168 spots for SRR7050355/SRR7050355.sra 2020-06-29T14:17:38 prefetch.2.10.7: 1) Downloading 'SRR7050356'... 2020-06-29T14:17:38 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:18:35 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:18:36 prefetch.2.10.7: 'SRR7050356' is valid 2020-06-29T14:18:36 prefetch.2.10.7: 1) 'SRR7050356' was downloaded successfully 2020-06-29T14:18:36 prefetch.2.10.7: 'SRR7050356' has 0 unresolved dependencies Read 11607515 spots for SRR7050356/SRR7050356.sra Written 11607515 spots for SRR7050356/SRR7050356.sra 2020-06-29T14:19:31 prefetch.2.10.7: 1) Downloading 'SRR7050357'... 2020-06-29T14:19:31 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:20:13 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:20:14 prefetch.2.10.7: 'SRR7050357' is valid 2020-06-29T14:20:14 prefetch.2.10.7: 1) 'SRR7050357' was downloaded successfully 2020-06-29T14:20:14 prefetch.2.10.7: 'SRR7050357' has 0 unresolved dependencies Read 11689729 spots for SRR7050357/SRR7050357.sra Written 11689729 spots for SRR7050357/SRR7050357.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:27 34931412 reads; of these: 34931412 (100.00%) were unpaired; of these: 1349260 (3.86%) aligned 0 times 25177554 (72.08%) aligned exactly 1 time 8404598 (24.06%) aligned >1 times 96.14% overall alignment rate Time searching: 00:10:27 Overall time: 00:10:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 3319715 / 33582152 = 0.0989 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:46:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981657/SRX3981657.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981657/SRX3981657.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981657/SRX3981657.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981657/SRX3981657.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:46:51: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:46:51: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:46:57: 1000000 INFO @ Mon, 29 Jun 2020 23:47:04: 2000000 INFO @ Mon, 29 Jun 2020 23:47:10: 3000000 INFO @ Mon, 29 Jun 2020 23:47:16: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:47:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981657/SRX3981657.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981657/SRX3981657.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981657/SRX3981657.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981657/SRX3981657.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:47:21: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:47:21: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:47:22: 5000000 INFO @ Mon, 29 Jun 2020 23:47:28: 1000000 INFO @ Mon, 29 Jun 2020 23:47:29: 6000000 INFO @ Mon, 29 Jun 2020 23:47:35: 2000000 INFO @ Mon, 29 Jun 2020 23:47:36: 7000000 INFO @ Mon, 29 Jun 2020 23:47:42: 3000000 INFO @ Mon, 29 Jun 2020 23:47:42: 8000000 INFO @ Mon, 29 Jun 2020 23:47:49: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:47:49: 9000000 INFO @ Mon, 29 Jun 2020 23:47:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981657/SRX3981657.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981657/SRX3981657.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981657/SRX3981657.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981657/SRX3981657.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:47:51: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:47:51: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:47:57: 5000000 INFO @ Mon, 29 Jun 2020 23:47:57: 10000000 INFO @ Mon, 29 Jun 2020 23:47:59: 1000000 INFO @ Mon, 29 Jun 2020 23:48:05: 6000000 INFO @ Mon, 29 Jun 2020 23:48:05: 11000000 INFO @ Mon, 29 Jun 2020 23:48:07: 2000000 INFO @ Mon, 29 Jun 2020 23:48:12: 7000000 INFO @ Mon, 29 Jun 2020 23:48:13: 12000000 INFO @ Mon, 29 Jun 2020 23:48:16: 3000000 INFO @ Mon, 29 Jun 2020 23:48:20: 8000000 INFO @ Mon, 29 Jun 2020 23:48:21: 13000000 INFO @ Mon, 29 Jun 2020 23:48:24: 4000000 INFO @ Mon, 29 Jun 2020 23:48:28: 9000000 INFO @ Mon, 29 Jun 2020 23:48:29: 14000000 INFO @ Mon, 29 Jun 2020 23:48:32: 5000000 INFO @ Mon, 29 Jun 2020 23:48:36: 10000000 INFO @ Mon, 29 Jun 2020 23:48:37: 15000000 INFO @ Mon, 29 Jun 2020 23:48:41: 6000000 INFO @ Mon, 29 Jun 2020 23:48:44: 11000000 INFO @ Mon, 29 Jun 2020 23:48:45: 16000000 INFO @ Mon, 29 Jun 2020 23:48:50: 7000000 INFO @ Mon, 29 Jun 2020 23:48:52: 12000000 INFO @ Mon, 29 Jun 2020 23:48:53: 17000000 INFO @ Mon, 29 Jun 2020 23:48:58: 8000000 INFO @ Mon, 29 Jun 2020 23:49:00: 13000000 INFO @ Mon, 29 Jun 2020 23:49:01: 18000000 INFO @ Mon, 29 Jun 2020 23:49:07: 9000000 INFO @ Mon, 29 Jun 2020 23:49:08: 14000000 INFO @ Mon, 29 Jun 2020 23:49:09: 19000000 INFO @ Mon, 29 Jun 2020 23:49:15: 10000000 INFO @ Mon, 29 Jun 2020 23:49:16: 15000000 INFO @ Mon, 29 Jun 2020 23:49:17: 20000000 INFO @ Mon, 29 Jun 2020 23:49:24: 16000000 INFO @ Mon, 29 Jun 2020 23:49:24: 11000000 INFO @ Mon, 29 Jun 2020 23:49:25: 21000000 INFO @ Mon, 29 Jun 2020 23:49:32: 17000000 INFO @ Mon, 29 Jun 2020 23:49:32: 12000000 INFO @ Mon, 29 Jun 2020 23:49:33: 22000000 INFO @ Mon, 29 Jun 2020 23:49:40: 18000000 INFO @ Mon, 29 Jun 2020 23:49:41: 23000000 INFO @ Mon, 29 Jun 2020 23:49:41: 13000000 INFO @ Mon, 29 Jun 2020 23:49:48: 19000000 INFO @ Mon, 29 Jun 2020 23:49:49: 24000000 INFO @ Mon, 29 Jun 2020 23:49:50: 14000000 INFO @ Mon, 29 Jun 2020 23:49:55: 20000000 INFO @ Mon, 29 Jun 2020 23:49:57: 25000000 INFO @ Mon, 29 Jun 2020 23:49:58: 15000000 INFO @ Mon, 29 Jun 2020 23:50:03: 21000000 INFO @ Mon, 29 Jun 2020 23:50:05: 26000000 INFO @ Mon, 29 Jun 2020 23:50:07: 16000000 INFO @ Mon, 29 Jun 2020 23:50:11: 22000000 INFO @ Mon, 29 Jun 2020 23:50:13: 27000000 INFO @ Mon, 29 Jun 2020 23:50:15: 17000000 INFO @ Mon, 29 Jun 2020 23:50:19: 23000000 INFO @ Mon, 29 Jun 2020 23:50:21: 28000000 INFO @ Mon, 29 Jun 2020 23:50:24: 18000000 INFO @ Mon, 29 Jun 2020 23:50:27: 24000000 INFO @ Mon, 29 Jun 2020 23:50:28: 29000000 INFO @ Mon, 29 Jun 2020 23:50:32: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:50:35: 25000000 INFO @ Mon, 29 Jun 2020 23:50:36: 30000000 INFO @ Mon, 29 Jun 2020 23:50:38: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:50:38: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:50:38: #1 total tags in treatment: 30262437 INFO @ Mon, 29 Jun 2020 23:50:38: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:50:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:50:39: #1 tags after filtering in treatment: 30262437 INFO @ Mon, 29 Jun 2020 23:50:39: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:50:39: #1 finished! INFO @ Mon, 29 Jun 2020 23:50:39: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:50:39: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:50:41: 20000000 INFO @ Mon, 29 Jun 2020 23:50:41: #2 number of paired peaks: 31 WARNING @ Mon, 29 Jun 2020 23:50:41: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:50:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981657/SRX3981657.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981657/SRX3981657.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981657/SRX3981657.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981657/SRX3981657.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:50:43: 26000000 INFO @ Mon, 29 Jun 2020 23:50:49: 21000000 INFO @ Mon, 29 Jun 2020 23:50:51: 27000000 INFO @ Mon, 29 Jun 2020 23:50:58: 22000000 INFO @ Mon, 29 Jun 2020 23:50:58: 28000000 INFO @ Mon, 29 Jun 2020 23:51:06: 29000000 INFO @ Mon, 29 Jun 2020 23:51:06: 23000000 INFO @ Mon, 29 Jun 2020 23:51:14: 30000000 INFO @ Mon, 29 Jun 2020 23:51:15: 24000000 INFO @ Mon, 29 Jun 2020 23:51:16: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:51:16: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:51:16: #1 total tags in treatment: 30262437 INFO @ Mon, 29 Jun 2020 23:51:16: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:51:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:51:16: #1 tags after filtering in treatment: 30262437 INFO @ Mon, 29 Jun 2020 23:51:16: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:51:16: #1 finished! INFO @ Mon, 29 Jun 2020 23:51:16: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:51:16: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:51:18: #2 number of paired peaks: 31 WARNING @ Mon, 29 Jun 2020 23:51:18: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:51:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981657/SRX3981657.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981657/SRX3981657.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981657/SRX3981657.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981657/SRX3981657.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:51:22: 25000000 INFO @ Mon, 29 Jun 2020 23:51:30: 26000000 INFO @ Mon, 29 Jun 2020 23:51:38: 27000000 BigWig に変換しました。 INFO @ Mon, 29 Jun 2020 23:51:45: 28000000 INFO @ Mon, 29 Jun 2020 23:51:53: 29000000 INFO @ Mon, 29 Jun 2020 23:52:00: 30000000 INFO @ Mon, 29 Jun 2020 23:52:02: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:52:02: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:52:02: #1 total tags in treatment: 30262437 INFO @ Mon, 29 Jun 2020 23:52:02: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:52:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:52:03: #1 tags after filtering in treatment: 30262437 INFO @ Mon, 29 Jun 2020 23:52:03: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:52:03: #1 finished! INFO @ Mon, 29 Jun 2020 23:52:03: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:52:03: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:52:05: #2 number of paired peaks: 31 WARNING @ Mon, 29 Jun 2020 23:52:05: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:52:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981657/SRX3981657.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981657/SRX3981657.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981657/SRX3981657.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981657/SRX3981657.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling