Job ID = 6528037 SRX = SRX3981656 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:32:06 prefetch.2.10.7: 1) Downloading 'SRR7050352'... 2020-06-29T14:32:06 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:33:13 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:33:13 prefetch.2.10.7: 'SRR7050352' is valid 2020-06-29T14:33:13 prefetch.2.10.7: 1) 'SRR7050352' was downloaded successfully 2020-06-29T14:33:13 prefetch.2.10.7: 'SRR7050352' has 0 unresolved dependencies Read 10833793 spots for SRR7050352/SRR7050352.sra Written 10833793 spots for SRR7050352/SRR7050352.sra 2020-06-29T14:34:00 prefetch.2.10.7: 1) Downloading 'SRR7050353'... 2020-06-29T14:34:00 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:35:21 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:35:21 prefetch.2.10.7: 'SRR7050353' is valid 2020-06-29T14:35:21 prefetch.2.10.7: 1) 'SRR7050353' was downloaded successfully 2020-06-29T14:35:21 prefetch.2.10.7: 'SRR7050353' has 0 unresolved dependencies Read 10816974 spots for SRR7050353/SRR7050353.sra Written 10816974 spots for SRR7050353/SRR7050353.sra 2020-06-29T14:36:07 prefetch.2.10.7: 1) Downloading 'SRR7050354'... 2020-06-29T14:36:07 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:37:48 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:37:49 prefetch.2.10.7: 'SRR7050354' is valid 2020-06-29T14:37:49 prefetch.2.10.7: 1) 'SRR7050354' was downloaded successfully 2020-06-29T14:37:49 prefetch.2.10.7: 'SRR7050354' has 0 unresolved dependencies Read 10889610 spots for SRR7050354/SRR7050354.sra Written 10889610 spots for SRR7050354/SRR7050354.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:08 32540377 reads; of these: 32540377 (100.00%) were unpaired; of these: 920316 (2.83%) aligned 0 times 23301362 (71.61%) aligned exactly 1 time 8318699 (25.56%) aligned >1 times 97.17% overall alignment rate Time searching: 00:11:08 Overall time: 00:11:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 3181239 / 31620061 = 0.1006 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:03:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981656/SRX3981656.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981656/SRX3981656.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981656/SRX3981656.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981656/SRX3981656.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:03:09: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:03:09: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:03:14: 1000000 INFO @ Tue, 30 Jun 2020 00:03:20: 2000000 INFO @ Tue, 30 Jun 2020 00:03:25: 3000000 INFO @ Tue, 30 Jun 2020 00:03:31: 4000000 INFO @ Tue, 30 Jun 2020 00:03:36: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:03:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981656/SRX3981656.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981656/SRX3981656.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981656/SRX3981656.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981656/SRX3981656.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:03:39: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:03:39: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:03:41: 6000000 INFO @ Tue, 30 Jun 2020 00:03:44: 1000000 INFO @ Tue, 30 Jun 2020 00:03:47: 7000000 INFO @ Tue, 30 Jun 2020 00:03:50: 2000000 INFO @ Tue, 30 Jun 2020 00:03:53: 8000000 INFO @ Tue, 30 Jun 2020 00:03:55: 3000000 INFO @ Tue, 30 Jun 2020 00:03:58: 9000000 INFO @ Tue, 30 Jun 2020 00:04:01: 4000000 INFO @ Tue, 30 Jun 2020 00:04:04: 10000000 INFO @ Tue, 30 Jun 2020 00:04:07: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:04:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981656/SRX3981656.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981656/SRX3981656.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981656/SRX3981656.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981656/SRX3981656.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:04:09: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:04:09: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:04:10: 11000000 INFO @ Tue, 30 Jun 2020 00:04:12: 6000000 INFO @ Tue, 30 Jun 2020 00:04:14: 1000000 INFO @ Tue, 30 Jun 2020 00:04:15: 12000000 INFO @ Tue, 30 Jun 2020 00:04:18: 7000000 INFO @ Tue, 30 Jun 2020 00:04:19: 2000000 INFO @ Tue, 30 Jun 2020 00:04:21: 13000000 INFO @ Tue, 30 Jun 2020 00:04:23: 8000000 INFO @ Tue, 30 Jun 2020 00:04:24: 3000000 INFO @ Tue, 30 Jun 2020 00:04:26: 14000000 INFO @ Tue, 30 Jun 2020 00:04:28: 4000000 INFO @ Tue, 30 Jun 2020 00:04:29: 9000000 INFO @ Tue, 30 Jun 2020 00:04:32: 15000000 INFO @ Tue, 30 Jun 2020 00:04:33: 5000000 INFO @ Tue, 30 Jun 2020 00:04:34: 10000000 INFO @ Tue, 30 Jun 2020 00:04:37: 16000000 INFO @ Tue, 30 Jun 2020 00:04:38: 6000000 INFO @ Tue, 30 Jun 2020 00:04:40: 11000000 INFO @ Tue, 30 Jun 2020 00:04:43: 17000000 INFO @ Tue, 30 Jun 2020 00:04:43: 7000000 INFO @ Tue, 30 Jun 2020 00:04:45: 12000000 INFO @ Tue, 30 Jun 2020 00:04:48: 8000000 INFO @ Tue, 30 Jun 2020 00:04:48: 18000000 INFO @ Tue, 30 Jun 2020 00:04:51: 13000000 INFO @ Tue, 30 Jun 2020 00:04:53: 9000000 INFO @ Tue, 30 Jun 2020 00:04:54: 19000000 INFO @ Tue, 30 Jun 2020 00:04:56: 14000000 INFO @ Tue, 30 Jun 2020 00:04:57: 10000000 INFO @ Tue, 30 Jun 2020 00:04:59: 20000000 INFO @ Tue, 30 Jun 2020 00:05:02: 15000000 INFO @ Tue, 30 Jun 2020 00:05:02: 11000000 INFO @ Tue, 30 Jun 2020 00:05:05: 21000000 INFO @ Tue, 30 Jun 2020 00:05:07: 12000000 INFO @ Tue, 30 Jun 2020 00:05:07: 16000000 INFO @ Tue, 30 Jun 2020 00:05:10: 22000000 INFO @ Tue, 30 Jun 2020 00:05:12: 13000000 INFO @ Tue, 30 Jun 2020 00:05:13: 17000000 INFO @ Tue, 30 Jun 2020 00:05:16: 23000000 INFO @ Tue, 30 Jun 2020 00:05:17: 14000000 INFO @ Tue, 30 Jun 2020 00:05:18: 18000000 INFO @ Tue, 30 Jun 2020 00:05:21: 15000000 INFO @ Tue, 30 Jun 2020 00:05:22: 24000000 INFO @ Tue, 30 Jun 2020 00:05:24: 19000000 INFO @ Tue, 30 Jun 2020 00:05:26: 16000000 INFO @ Tue, 30 Jun 2020 00:05:28: 25000000 INFO @ Tue, 30 Jun 2020 00:05:29: 20000000 INFO @ Tue, 30 Jun 2020 00:05:31: 17000000 INFO @ Tue, 30 Jun 2020 00:05:33: 26000000 INFO @ Tue, 30 Jun 2020 00:05:35: 21000000 INFO @ Tue, 30 Jun 2020 00:05:36: 18000000 INFO @ Tue, 30 Jun 2020 00:05:39: 27000000 INFO @ Tue, 30 Jun 2020 00:05:41: 19000000 INFO @ Tue, 30 Jun 2020 00:05:41: 22000000 INFO @ Tue, 30 Jun 2020 00:05:44: 28000000 INFO @ Tue, 30 Jun 2020 00:05:45: 20000000 INFO @ Tue, 30 Jun 2020 00:05:46: 23000000 INFO @ Tue, 30 Jun 2020 00:05:47: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:05:47: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:05:47: #1 total tags in treatment: 28438822 INFO @ Tue, 30 Jun 2020 00:05:47: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:05:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:05:47: #1 tags after filtering in treatment: 28438822 INFO @ Tue, 30 Jun 2020 00:05:47: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:05:47: #1 finished! INFO @ Tue, 30 Jun 2020 00:05:47: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:05:47: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:05:49: #2 number of paired peaks: 42 WARNING @ Tue, 30 Jun 2020 00:05:49: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:05:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981656/SRX3981656.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981656/SRX3981656.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981656/SRX3981656.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981656/SRX3981656.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:05:50: 21000000 INFO @ Tue, 30 Jun 2020 00:05:52: 24000000 INFO @ Tue, 30 Jun 2020 00:05:55: 22000000 INFO @ Tue, 30 Jun 2020 00:05:57: 25000000 INFO @ Tue, 30 Jun 2020 00:06:00: 23000000 INFO @ Tue, 30 Jun 2020 00:06:03: 26000000 INFO @ Tue, 30 Jun 2020 00:06:05: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:06:08: 27000000 INFO @ Tue, 30 Jun 2020 00:06:09: 25000000 INFO @ Tue, 30 Jun 2020 00:06:13: 28000000 INFO @ Tue, 30 Jun 2020 00:06:14: 26000000 INFO @ Tue, 30 Jun 2020 00:06:16: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:06:16: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:06:16: #1 total tags in treatment: 28438822 INFO @ Tue, 30 Jun 2020 00:06:16: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:06:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:06:16: #1 tags after filtering in treatment: 28438822 INFO @ Tue, 30 Jun 2020 00:06:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:06:16: #1 finished! INFO @ Tue, 30 Jun 2020 00:06:16: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:06:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:06:18: #2 number of paired peaks: 42 WARNING @ Tue, 30 Jun 2020 00:06:18: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:06:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981656/SRX3981656.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981656/SRX3981656.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981656/SRX3981656.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981656/SRX3981656.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:06:19: 27000000 INFO @ Tue, 30 Jun 2020 00:06:23: 28000000 INFO @ Tue, 30 Jun 2020 00:06:26: #1 tag size is determined as 51 bps INFO @ Tue, 30 Jun 2020 00:06:26: #1 tag size = 51 INFO @ Tue, 30 Jun 2020 00:06:26: #1 total tags in treatment: 28438822 INFO @ Tue, 30 Jun 2020 00:06:26: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:06:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:06:26: #1 tags after filtering in treatment: 28438822 INFO @ Tue, 30 Jun 2020 00:06:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:06:26: #1 finished! INFO @ Tue, 30 Jun 2020 00:06:26: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:06:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:06:28: #2 number of paired peaks: 42 WARNING @ Tue, 30 Jun 2020 00:06:28: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:06:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981656/SRX3981656.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981656/SRX3981656.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981656/SRX3981656.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981656/SRX3981656.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。