Job ID = 6528032 SRX = SRX3981649 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:13:21 prefetch.2.10.7: 1) Downloading 'SRR7050331'... 2020-06-29T14:13:21 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:14:08 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:14:09 prefetch.2.10.7: 'SRR7050331' is valid 2020-06-29T14:14:09 prefetch.2.10.7: 1) 'SRR7050331' was downloaded successfully 2020-06-29T14:14:09 prefetch.2.10.7: 'SRR7050331' has 0 unresolved dependencies Read 11864553 spots for SRR7050331/SRR7050331.sra Written 11864553 spots for SRR7050331/SRR7050331.sra 2020-06-29T14:15:07 prefetch.2.10.7: 1) Downloading 'SRR7050332'... 2020-06-29T14:15:07 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:15:55 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:15:55 prefetch.2.10.7: 'SRR7050332' is valid 2020-06-29T14:15:55 prefetch.2.10.7: 1) 'SRR7050332' was downloaded successfully 2020-06-29T14:15:55 prefetch.2.10.7: 'SRR7050332' has 0 unresolved dependencies Read 11832783 spots for SRR7050332/SRR7050332.sra Written 11832783 spots for SRR7050332/SRR7050332.sra 2020-06-29T14:16:54 prefetch.2.10.7: 1) Downloading 'SRR7050333'... 2020-06-29T14:16:54 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:17:57 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:17:57 prefetch.2.10.7: 'SRR7050333' is valid 2020-06-29T14:17:57 prefetch.2.10.7: 1) 'SRR7050333' was downloaded successfully 2020-06-29T14:17:57 prefetch.2.10.7: 'SRR7050333' has 0 unresolved dependencies Read 11916889 spots for SRR7050333/SRR7050333.sra Written 11916889 spots for SRR7050333/SRR7050333.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:49 35614225 reads; of these: 35614225 (100.00%) were unpaired; of these: 1417028 (3.98%) aligned 0 times 24907506 (69.94%) aligned exactly 1 time 9289691 (26.08%) aligned >1 times 96.02% overall alignment rate Time searching: 00:11:49 Overall time: 00:11:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 3699073 / 34197197 = 0.1082 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:50:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981649/SRX3981649.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981649/SRX3981649.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981649/SRX3981649.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981649/SRX3981649.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:50:02: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:50:02: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:50:09: 1000000 INFO @ Mon, 29 Jun 2020 23:50:15: 2000000 INFO @ Mon, 29 Jun 2020 23:50:22: 3000000 INFO @ Mon, 29 Jun 2020 23:50:29: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:50:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981649/SRX3981649.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981649/SRX3981649.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981649/SRX3981649.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981649/SRX3981649.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:50:32: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:50:32: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:50:35: 5000000 INFO @ Mon, 29 Jun 2020 23:50:40: 1000000 INFO @ Mon, 29 Jun 2020 23:50:43: 6000000 INFO @ Mon, 29 Jun 2020 23:50:47: 2000000 INFO @ Mon, 29 Jun 2020 23:50:50: 7000000 INFO @ Mon, 29 Jun 2020 23:50:55: 3000000 INFO @ Mon, 29 Jun 2020 23:50:57: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:51:02: 4000000 INFO @ Mon, 29 Jun 2020 23:51:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981649/SRX3981649.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981649/SRX3981649.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981649/SRX3981649.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981649/SRX3981649.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:51:02: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:51:02: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:51:04: 9000000 INFO @ Mon, 29 Jun 2020 23:51:10: 1000000 INFO @ Mon, 29 Jun 2020 23:51:10: 5000000 INFO @ Mon, 29 Jun 2020 23:51:12: 10000000 INFO @ Mon, 29 Jun 2020 23:51:17: 2000000 INFO @ Mon, 29 Jun 2020 23:51:18: 6000000 INFO @ Mon, 29 Jun 2020 23:51:19: 11000000 INFO @ Mon, 29 Jun 2020 23:51:25: 3000000 INFO @ Mon, 29 Jun 2020 23:51:26: 7000000 INFO @ Mon, 29 Jun 2020 23:51:27: 12000000 INFO @ Mon, 29 Jun 2020 23:51:32: 4000000 INFO @ Mon, 29 Jun 2020 23:51:33: 8000000 INFO @ Mon, 29 Jun 2020 23:51:35: 13000000 INFO @ Mon, 29 Jun 2020 23:51:39: 5000000 INFO @ Mon, 29 Jun 2020 23:51:41: 9000000 INFO @ Mon, 29 Jun 2020 23:51:42: 14000000 INFO @ Mon, 29 Jun 2020 23:51:47: 6000000 INFO @ Mon, 29 Jun 2020 23:51:48: 10000000 INFO @ Mon, 29 Jun 2020 23:51:50: 15000000 INFO @ Mon, 29 Jun 2020 23:51:54: 7000000 INFO @ Mon, 29 Jun 2020 23:51:56: 11000000 INFO @ Mon, 29 Jun 2020 23:51:57: 16000000 INFO @ Mon, 29 Jun 2020 23:52:02: 8000000 INFO @ Mon, 29 Jun 2020 23:52:03: 12000000 INFO @ Mon, 29 Jun 2020 23:52:05: 17000000 INFO @ Mon, 29 Jun 2020 23:52:09: 9000000 INFO @ Mon, 29 Jun 2020 23:52:11: 13000000 INFO @ Mon, 29 Jun 2020 23:52:12: 18000000 INFO @ Mon, 29 Jun 2020 23:52:16: 10000000 INFO @ Mon, 29 Jun 2020 23:52:18: 14000000 INFO @ Mon, 29 Jun 2020 23:52:20: 19000000 INFO @ Mon, 29 Jun 2020 23:52:24: 11000000 INFO @ Mon, 29 Jun 2020 23:52:26: 15000000 INFO @ Mon, 29 Jun 2020 23:52:27: 20000000 INFO @ Mon, 29 Jun 2020 23:52:31: 12000000 INFO @ Mon, 29 Jun 2020 23:52:33: 16000000 INFO @ Mon, 29 Jun 2020 23:52:35: 21000000 INFO @ Mon, 29 Jun 2020 23:52:39: 13000000 INFO @ Mon, 29 Jun 2020 23:52:41: 17000000 INFO @ Mon, 29 Jun 2020 23:52:43: 22000000 INFO @ Mon, 29 Jun 2020 23:52:46: 14000000 INFO @ Mon, 29 Jun 2020 23:52:49: 18000000 INFO @ Mon, 29 Jun 2020 23:52:50: 23000000 INFO @ Mon, 29 Jun 2020 23:52:53: 15000000 INFO @ Mon, 29 Jun 2020 23:52:56: 19000000 INFO @ Mon, 29 Jun 2020 23:52:58: 24000000 INFO @ Mon, 29 Jun 2020 23:53:01: 16000000 INFO @ Mon, 29 Jun 2020 23:53:04: 20000000 INFO @ Mon, 29 Jun 2020 23:53:05: 25000000 INFO @ Mon, 29 Jun 2020 23:53:08: 17000000 INFO @ Mon, 29 Jun 2020 23:53:11: 21000000 INFO @ Mon, 29 Jun 2020 23:53:12: 26000000 INFO @ Mon, 29 Jun 2020 23:53:16: 18000000 INFO @ Mon, 29 Jun 2020 23:53:19: 22000000 INFO @ Mon, 29 Jun 2020 23:53:20: 27000000 INFO @ Mon, 29 Jun 2020 23:53:23: 19000000 INFO @ Mon, 29 Jun 2020 23:53:26: 23000000 INFO @ Mon, 29 Jun 2020 23:53:27: 28000000 INFO @ Mon, 29 Jun 2020 23:53:30: 20000000 INFO @ Mon, 29 Jun 2020 23:53:34: 24000000 INFO @ Mon, 29 Jun 2020 23:53:35: 29000000 INFO @ Mon, 29 Jun 2020 23:53:38: 21000000 INFO @ Mon, 29 Jun 2020 23:53:41: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:53:43: 30000000 INFO @ Mon, 29 Jun 2020 23:53:45: 22000000 INFO @ Mon, 29 Jun 2020 23:53:46: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:53:46: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:53:46: #1 total tags in treatment: 30498124 INFO @ Mon, 29 Jun 2020 23:53:46: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:53:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:53:47: #1 tags after filtering in treatment: 30498124 INFO @ Mon, 29 Jun 2020 23:53:47: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:53:47: #1 finished! INFO @ Mon, 29 Jun 2020 23:53:47: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:53:47: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:53:48: 26000000 INFO @ Mon, 29 Jun 2020 23:53:49: #2 number of paired peaks: 29 WARNING @ Mon, 29 Jun 2020 23:53:49: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:53:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981649/SRX3981649.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981649/SRX3981649.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981649/SRX3981649.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981649/SRX3981649.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:53:52: 23000000 INFO @ Mon, 29 Jun 2020 23:53:56: 27000000 INFO @ Mon, 29 Jun 2020 23:53:59: 24000000 INFO @ Mon, 29 Jun 2020 23:54:03: 28000000 INFO @ Mon, 29 Jun 2020 23:54:06: 25000000 INFO @ Mon, 29 Jun 2020 23:54:10: 29000000 INFO @ Mon, 29 Jun 2020 23:54:13: 26000000 INFO @ Mon, 29 Jun 2020 23:54:17: 30000000 INFO @ Mon, 29 Jun 2020 23:54:20: 27000000 INFO @ Mon, 29 Jun 2020 23:54:21: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:54:21: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:54:21: #1 total tags in treatment: 30498124 INFO @ Mon, 29 Jun 2020 23:54:21: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:54:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:54:21: #1 tags after filtering in treatment: 30498124 INFO @ Mon, 29 Jun 2020 23:54:21: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:54:21: #1 finished! INFO @ Mon, 29 Jun 2020 23:54:21: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:54:21: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:54:23: #2 number of paired peaks: 29 WARNING @ Mon, 29 Jun 2020 23:54:23: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:54:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981649/SRX3981649.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981649/SRX3981649.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981649/SRX3981649.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981649/SRX3981649.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:54:26: 28000000 INFO @ Mon, 29 Jun 2020 23:54:33: 29000000 INFO @ Mon, 29 Jun 2020 23:54:40: 30000000 INFO @ Mon, 29 Jun 2020 23:54:43: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:54:43: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:54:43: #1 total tags in treatment: 30498124 INFO @ Mon, 29 Jun 2020 23:54:43: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:54:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:54:44: #1 tags after filtering in treatment: 30498124 INFO @ Mon, 29 Jun 2020 23:54:44: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:54:44: #1 finished! INFO @ Mon, 29 Jun 2020 23:54:44: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:54:44: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:54:45: #2 number of paired peaks: 29 WARNING @ Mon, 29 Jun 2020 23:54:45: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:54:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981649/SRX3981649.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981649/SRX3981649.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981649/SRX3981649.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981649/SRX3981649.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。