Job ID = 2590364 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 14,716,309 reads read : 29,432,618 reads written : 14,716,309 reads 0-length : 14,716,309 spots read : 14,588,187 reads read : 29,176,374 reads written : 14,588,187 reads 0-length : 14,588,187 spots read : 14,567,985 reads read : 29,135,970 reads written : 14,567,985 reads 0-length : 14,567,985 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:07 43872481 reads; of these: 43872481 (100.00%) were unpaired; of these: 1335011 (3.04%) aligned 0 times 31555688 (71.93%) aligned exactly 1 time 10981782 (25.03%) aligned >1 times 96.96% overall alignment rate Time searching: 00:16:07 Overall time: 00:16:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 4785130 / 42537470 = 0.1125 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 21:41:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981636/SRX3981636.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981636/SRX3981636.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981636/SRX3981636.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981636/SRX3981636.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 21:41:24: #1 read tag files... INFO @ Mon, 12 Aug 2019 21:41:24: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 21:41:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981636/SRX3981636.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981636/SRX3981636.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981636/SRX3981636.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981636/SRX3981636.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 21:41:25: #1 read tag files... INFO @ Mon, 12 Aug 2019 21:41:25: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 21:41:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981636/SRX3981636.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981636/SRX3981636.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981636/SRX3981636.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981636/SRX3981636.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 21:41:26: #1 read tag files... INFO @ Mon, 12 Aug 2019 21:41:26: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 21:41:32: 1000000 INFO @ Mon, 12 Aug 2019 21:41:33: 1000000 INFO @ Mon, 12 Aug 2019 21:41:35: 1000000 INFO @ Mon, 12 Aug 2019 21:41:41: 2000000 INFO @ Mon, 12 Aug 2019 21:41:41: 2000000 INFO @ Mon, 12 Aug 2019 21:41:46: 2000000 INFO @ Mon, 12 Aug 2019 21:41:49: 3000000 INFO @ Mon, 12 Aug 2019 21:41:50: 3000000 INFO @ Mon, 12 Aug 2019 21:41:56: 3000000 INFO @ Mon, 12 Aug 2019 21:41:57: 4000000 INFO @ Mon, 12 Aug 2019 21:41:58: 4000000 INFO @ Mon, 12 Aug 2019 21:42:04: 5000000 INFO @ Mon, 12 Aug 2019 21:42:06: 5000000 INFO @ Mon, 12 Aug 2019 21:42:06: 4000000 INFO @ Mon, 12 Aug 2019 21:42:12: 6000000 INFO @ Mon, 12 Aug 2019 21:42:14: 6000000 INFO @ Mon, 12 Aug 2019 21:42:16: 5000000 INFO @ Mon, 12 Aug 2019 21:42:19: 7000000 INFO @ Mon, 12 Aug 2019 21:42:23: 7000000 INFO @ Mon, 12 Aug 2019 21:42:26: 6000000 INFO @ Mon, 12 Aug 2019 21:42:26: 8000000 INFO @ Mon, 12 Aug 2019 21:42:31: 8000000 INFO @ Mon, 12 Aug 2019 21:42:34: 9000000 INFO @ Mon, 12 Aug 2019 21:42:35: 7000000 INFO @ Mon, 12 Aug 2019 21:42:39: 9000000 INFO @ Mon, 12 Aug 2019 21:42:41: 10000000 INFO @ Mon, 12 Aug 2019 21:42:43: 8000000 INFO @ Mon, 12 Aug 2019 21:42:47: 10000000 INFO @ Mon, 12 Aug 2019 21:42:49: 11000000 INFO @ Mon, 12 Aug 2019 21:42:52: 9000000 INFO @ Mon, 12 Aug 2019 21:42:55: 11000000 INFO @ Mon, 12 Aug 2019 21:42:56: 12000000 INFO @ Mon, 12 Aug 2019 21:43:00: 10000000 INFO @ Mon, 12 Aug 2019 21:43:03: 12000000 INFO @ Mon, 12 Aug 2019 21:43:03: 13000000 INFO @ Mon, 12 Aug 2019 21:43:08: 11000000 INFO @ Mon, 12 Aug 2019 21:43:11: 14000000 INFO @ Mon, 12 Aug 2019 21:43:11: 13000000 INFO @ Mon, 12 Aug 2019 21:43:17: 12000000 INFO @ Mon, 12 Aug 2019 21:43:18: 15000000 INFO @ Mon, 12 Aug 2019 21:43:19: 14000000 INFO @ Mon, 12 Aug 2019 21:43:25: 13000000 INFO @ Mon, 12 Aug 2019 21:43:25: 16000000 INFO @ Mon, 12 Aug 2019 21:43:27: 15000000 INFO @ Mon, 12 Aug 2019 21:43:33: 17000000 INFO @ Mon, 12 Aug 2019 21:43:34: 14000000 INFO @ Mon, 12 Aug 2019 21:43:36: 16000000 INFO @ Mon, 12 Aug 2019 21:43:40: 18000000 INFO @ Mon, 12 Aug 2019 21:43:43: 15000000 INFO @ Mon, 12 Aug 2019 21:43:44: 17000000 INFO @ Mon, 12 Aug 2019 21:43:48: 19000000 INFO @ Mon, 12 Aug 2019 21:43:52: 18000000 INFO @ Mon, 12 Aug 2019 21:43:52: 16000000 INFO @ Mon, 12 Aug 2019 21:43:55: 20000000 INFO @ Mon, 12 Aug 2019 21:44:00: 19000000 INFO @ Mon, 12 Aug 2019 21:44:01: 17000000 INFO @ Mon, 12 Aug 2019 21:44:03: 21000000 INFO @ Mon, 12 Aug 2019 21:44:09: 20000000 INFO @ Mon, 12 Aug 2019 21:44:09: 18000000 INFO @ Mon, 12 Aug 2019 21:44:10: 22000000 INFO @ Mon, 12 Aug 2019 21:44:17: 21000000 INFO @ Mon, 12 Aug 2019 21:44:17: 19000000 INFO @ Mon, 12 Aug 2019 21:44:17: 23000000 INFO @ Mon, 12 Aug 2019 21:44:25: 24000000 INFO @ Mon, 12 Aug 2019 21:44:25: 22000000 INFO @ Mon, 12 Aug 2019 21:44:26: 20000000 INFO @ Mon, 12 Aug 2019 21:44:34: 23000000 INFO @ Mon, 12 Aug 2019 21:44:34: 25000000 INFO @ Mon, 12 Aug 2019 21:44:35: 21000000 INFO @ Mon, 12 Aug 2019 21:44:42: 24000000 INFO @ Mon, 12 Aug 2019 21:44:43: 26000000 INFO @ Mon, 12 Aug 2019 21:44:44: 22000000 INFO @ Mon, 12 Aug 2019 21:44:51: 25000000 INFO @ Mon, 12 Aug 2019 21:44:51: 27000000 INFO @ Mon, 12 Aug 2019 21:44:52: 23000000 INFO @ Mon, 12 Aug 2019 21:45:00: 26000000 INFO @ Mon, 12 Aug 2019 21:45:00: 28000000 INFO @ Mon, 12 Aug 2019 21:45:01: 24000000 INFO @ Mon, 12 Aug 2019 21:45:08: 27000000 INFO @ Mon, 12 Aug 2019 21:45:09: 29000000 INFO @ Mon, 12 Aug 2019 21:45:10: 25000000 INFO @ Mon, 12 Aug 2019 21:45:17: 28000000 INFO @ Mon, 12 Aug 2019 21:45:18: 30000000 INFO @ Mon, 12 Aug 2019 21:45:18: 26000000 INFO @ Mon, 12 Aug 2019 21:45:26: 29000000 INFO @ Mon, 12 Aug 2019 21:45:27: 31000000 INFO @ Mon, 12 Aug 2019 21:45:27: 27000000 INFO @ Mon, 12 Aug 2019 21:45:34: 30000000 INFO @ Mon, 12 Aug 2019 21:45:35: 32000000 INFO @ Mon, 12 Aug 2019 21:45:36: 28000000 INFO @ Mon, 12 Aug 2019 21:45:43: 31000000 INFO @ Mon, 12 Aug 2019 21:45:44: 33000000 INFO @ Mon, 12 Aug 2019 21:45:44: 29000000 INFO @ Mon, 12 Aug 2019 21:45:52: 32000000 INFO @ Mon, 12 Aug 2019 21:45:53: 34000000 INFO @ Mon, 12 Aug 2019 21:45:53: 30000000 INFO @ Mon, 12 Aug 2019 21:46:01: 33000000 INFO @ Mon, 12 Aug 2019 21:46:01: 35000000 INFO @ Mon, 12 Aug 2019 21:46:02: 31000000 INFO @ Mon, 12 Aug 2019 21:46:09: 34000000 INFO @ Mon, 12 Aug 2019 21:46:11: 36000000 INFO @ Mon, 12 Aug 2019 21:46:11: 32000000 INFO @ Mon, 12 Aug 2019 21:46:18: 35000000 INFO @ Mon, 12 Aug 2019 21:46:19: 33000000 INFO @ Mon, 12 Aug 2019 21:46:20: 37000000 INFO @ Mon, 12 Aug 2019 21:46:26: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 21:46:26: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 21:46:26: #1 total tags in treatment: 37752340 INFO @ Mon, 12 Aug 2019 21:46:26: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 21:46:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 21:46:27: 36000000 INFO @ Mon, 12 Aug 2019 21:46:27: #1 tags after filtering in treatment: 37752340 INFO @ Mon, 12 Aug 2019 21:46:27: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 21:46:27: #1 finished! INFO @ Mon, 12 Aug 2019 21:46:27: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 21:46:27: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 21:46:28: 34000000 INFO @ Mon, 12 Aug 2019 21:46:30: #2 number of paired peaks: 8 WARNING @ Mon, 12 Aug 2019 21:46:30: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 21:46:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981636/SRX3981636.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981636/SRX3981636.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981636/SRX3981636.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981636/SRX3981636.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 21:46:36: 37000000 INFO @ Mon, 12 Aug 2019 21:46:36: 35000000 INFO @ Mon, 12 Aug 2019 21:46:42: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 21:46:42: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 21:46:42: #1 total tags in treatment: 37752340 INFO @ Mon, 12 Aug 2019 21:46:42: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 21:46:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 21:46:43: #1 tags after filtering in treatment: 37752340 INFO @ Mon, 12 Aug 2019 21:46:43: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 21:46:43: #1 finished! INFO @ Mon, 12 Aug 2019 21:46:43: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 21:46:43: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 21:46:45: 36000000 INFO @ Mon, 12 Aug 2019 21:46:46: #2 number of paired peaks: 8 WARNING @ Mon, 12 Aug 2019 21:46:46: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 21:46:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981636/SRX3981636.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981636/SRX3981636.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981636/SRX3981636.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981636/SRX3981636.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 21:46:53: 37000000 INFO @ Mon, 12 Aug 2019 21:47:00: #1 tag size is determined as 51 bps INFO @ Mon, 12 Aug 2019 21:47:00: #1 tag size = 51 INFO @ Mon, 12 Aug 2019 21:47:00: #1 total tags in treatment: 37752340 INFO @ Mon, 12 Aug 2019 21:47:00: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 21:47:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 21:47:00: #1 tags after filtering in treatment: 37752340 INFO @ Mon, 12 Aug 2019 21:47:00: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 21:47:00: #1 finished! INFO @ Mon, 12 Aug 2019 21:47:00: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 21:47:00: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 21:47:03: #2 number of paired peaks: 8 WARNING @ Mon, 12 Aug 2019 21:47:03: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 21:47:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981636/SRX3981636.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981636/SRX3981636.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981636/SRX3981636.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981636/SRX3981636.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。