Job ID = 6528017 SRX = SRX3981617 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:52:33 prefetch.2.10.7: 1) Downloading 'SRR7050235'... 2020-06-29T13:52:33 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:53:30 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:53:30 prefetch.2.10.7: 'SRR7050235' is valid 2020-06-29T13:53:30 prefetch.2.10.7: 1) 'SRR7050235' was downloaded successfully 2020-06-29T13:53:30 prefetch.2.10.7: 'SRR7050235' has 0 unresolved dependencies Read 13014674 spots for SRR7050235/SRR7050235.sra Written 13014674 spots for SRR7050235/SRR7050235.sra 2020-06-29T13:54:35 prefetch.2.10.7: 1) Downloading 'SRR7050236'... 2020-06-29T13:54:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:55:32 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:55:33 prefetch.2.10.7: 'SRR7050236' is valid 2020-06-29T13:55:33 prefetch.2.10.7: 1) 'SRR7050236' was downloaded successfully 2020-06-29T13:55:33 prefetch.2.10.7: 'SRR7050236' has 0 unresolved dependencies Read 12788492 spots for SRR7050236/SRR7050236.sra Written 12788492 spots for SRR7050236/SRR7050236.sra 2020-06-29T13:56:35 prefetch.2.10.7: 1) Downloading 'SRR7050237'... 2020-06-29T13:56:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:58:02 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:58:03 prefetch.2.10.7: 'SRR7050237' is valid 2020-06-29T13:58:03 prefetch.2.10.7: 1) 'SRR7050237' was downloaded successfully 2020-06-29T13:58:03 prefetch.2.10.7: 'SRR7050237' has 0 unresolved dependencies Read 13005972 spots for SRR7050237/SRR7050237.sra Written 13005972 spots for SRR7050237/SRR7050237.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:47 38809138 reads; of these: 38809138 (100.00%) were unpaired; of these: 985513 (2.54%) aligned 0 times 28301473 (72.92%) aligned exactly 1 time 9522152 (24.54%) aligned >1 times 97.46% overall alignment rate Time searching: 00:11:47 Overall time: 00:11:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4215649 / 37823625 = 0.1115 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:32:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981617/SRX3981617.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981617/SRX3981617.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981617/SRX3981617.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981617/SRX3981617.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:32:28: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:32:28: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:32:33: 1000000 INFO @ Mon, 29 Jun 2020 23:32:38: 2000000 INFO @ Mon, 29 Jun 2020 23:32:42: 3000000 INFO @ Mon, 29 Jun 2020 23:32:47: 4000000 INFO @ Mon, 29 Jun 2020 23:32:52: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:32:56: 6000000 INFO @ Mon, 29 Jun 2020 23:32:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981617/SRX3981617.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981617/SRX3981617.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981617/SRX3981617.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981617/SRX3981617.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:32:58: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:32:58: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:33:01: 7000000 INFO @ Mon, 29 Jun 2020 23:33:03: 1000000 INFO @ Mon, 29 Jun 2020 23:33:06: 8000000 INFO @ Mon, 29 Jun 2020 23:33:08: 2000000 INFO @ Mon, 29 Jun 2020 23:33:11: 9000000 INFO @ Mon, 29 Jun 2020 23:33:13: 3000000 INFO @ Mon, 29 Jun 2020 23:33:17: 10000000 INFO @ Mon, 29 Jun 2020 23:33:18: 4000000 INFO @ Mon, 29 Jun 2020 23:33:22: 11000000 INFO @ Mon, 29 Jun 2020 23:33:23: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:33:26: 12000000 INFO @ Mon, 29 Jun 2020 23:33:28: 6000000 INFO @ Mon, 29 Jun 2020 23:33:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981617/SRX3981617.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981617/SRX3981617.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981617/SRX3981617.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981617/SRX3981617.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:33:28: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:33:28: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:33:31: 13000000 INFO @ Mon, 29 Jun 2020 23:33:33: 7000000 INFO @ Mon, 29 Jun 2020 23:33:33: 1000000 INFO @ Mon, 29 Jun 2020 23:33:36: 14000000 INFO @ Mon, 29 Jun 2020 23:33:38: 2000000 INFO @ Mon, 29 Jun 2020 23:33:38: 8000000 INFO @ Mon, 29 Jun 2020 23:33:41: 15000000 INFO @ Mon, 29 Jun 2020 23:33:43: 3000000 INFO @ Mon, 29 Jun 2020 23:33:43: 9000000 INFO @ Mon, 29 Jun 2020 23:33:47: 16000000 INFO @ Mon, 29 Jun 2020 23:33:48: 4000000 INFO @ Mon, 29 Jun 2020 23:33:48: 10000000 INFO @ Mon, 29 Jun 2020 23:33:52: 17000000 INFO @ Mon, 29 Jun 2020 23:33:52: 5000000 INFO @ Mon, 29 Jun 2020 23:33:53: 11000000 INFO @ Mon, 29 Jun 2020 23:33:57: 18000000 INFO @ Mon, 29 Jun 2020 23:33:57: 6000000 INFO @ Mon, 29 Jun 2020 23:33:58: 12000000 INFO @ Mon, 29 Jun 2020 23:34:02: 7000000 INFO @ Mon, 29 Jun 2020 23:34:02: 19000000 INFO @ Mon, 29 Jun 2020 23:34:03: 13000000 INFO @ Mon, 29 Jun 2020 23:34:06: 8000000 INFO @ Mon, 29 Jun 2020 23:34:07: 20000000 INFO @ Mon, 29 Jun 2020 23:34:08: 14000000 INFO @ Mon, 29 Jun 2020 23:34:11: 9000000 INFO @ Mon, 29 Jun 2020 23:34:12: 21000000 INFO @ Mon, 29 Jun 2020 23:34:13: 15000000 INFO @ Mon, 29 Jun 2020 23:34:16: 10000000 INFO @ Mon, 29 Jun 2020 23:34:17: 22000000 INFO @ Mon, 29 Jun 2020 23:34:18: 16000000 INFO @ Mon, 29 Jun 2020 23:34:20: 11000000 INFO @ Mon, 29 Jun 2020 23:34:22: 23000000 INFO @ Mon, 29 Jun 2020 23:34:23: 17000000 INFO @ Mon, 29 Jun 2020 23:34:25: 12000000 INFO @ Mon, 29 Jun 2020 23:34:27: 24000000 INFO @ Mon, 29 Jun 2020 23:34:28: 18000000 INFO @ Mon, 29 Jun 2020 23:34:30: 13000000 INFO @ Mon, 29 Jun 2020 23:34:32: 25000000 INFO @ Mon, 29 Jun 2020 23:34:33: 19000000 INFO @ Mon, 29 Jun 2020 23:34:34: 14000000 INFO @ Mon, 29 Jun 2020 23:34:37: 26000000 INFO @ Mon, 29 Jun 2020 23:34:38: 20000000 INFO @ Mon, 29 Jun 2020 23:34:39: 15000000 INFO @ Mon, 29 Jun 2020 23:34:42: 27000000 INFO @ Mon, 29 Jun 2020 23:34:43: 21000000 INFO @ Mon, 29 Jun 2020 23:34:44: 16000000 INFO @ Mon, 29 Jun 2020 23:34:47: 28000000 INFO @ Mon, 29 Jun 2020 23:34:48: 17000000 INFO @ Mon, 29 Jun 2020 23:34:48: 22000000 INFO @ Mon, 29 Jun 2020 23:34:52: 29000000 INFO @ Mon, 29 Jun 2020 23:34:53: 18000000 INFO @ Mon, 29 Jun 2020 23:34:53: 23000000 INFO @ Mon, 29 Jun 2020 23:34:57: 30000000 INFO @ Mon, 29 Jun 2020 23:34:58: 19000000 INFO @ Mon, 29 Jun 2020 23:34:58: 24000000 INFO @ Mon, 29 Jun 2020 23:35:02: 31000000 INFO @ Mon, 29 Jun 2020 23:35:02: 20000000 INFO @ Mon, 29 Jun 2020 23:35:03: 25000000 INFO @ Mon, 29 Jun 2020 23:35:07: 21000000 INFO @ Mon, 29 Jun 2020 23:35:07: 32000000 INFO @ Mon, 29 Jun 2020 23:35:08: 26000000 INFO @ Mon, 29 Jun 2020 23:35:12: 22000000 INFO @ Mon, 29 Jun 2020 23:35:13: 33000000 INFO @ Mon, 29 Jun 2020 23:35:14: 27000000 INFO @ Mon, 29 Jun 2020 23:35:16: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:35:16: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:35:16: #1 total tags in treatment: 33607976 INFO @ Mon, 29 Jun 2020 23:35:16: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:35:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:35:16: 23000000 INFO @ Mon, 29 Jun 2020 23:35:17: #1 tags after filtering in treatment: 33607976 INFO @ Mon, 29 Jun 2020 23:35:17: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:35:17: #1 finished! INFO @ Mon, 29 Jun 2020 23:35:17: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:35:17: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:35:18: 28000000 INFO @ Mon, 29 Jun 2020 23:35:19: #2 number of paired peaks: 39 WARNING @ Mon, 29 Jun 2020 23:35:19: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:35:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981617/SRX3981617.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981617/SRX3981617.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981617/SRX3981617.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981617/SRX3981617.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:35:21: 24000000 INFO @ Mon, 29 Jun 2020 23:35:23: 29000000 INFO @ Mon, 29 Jun 2020 23:35:26: 25000000 INFO @ Mon, 29 Jun 2020 23:35:28: 30000000 INFO @ Mon, 29 Jun 2020 23:35:31: 26000000 INFO @ Mon, 29 Jun 2020 23:35:33: 31000000 INFO @ Mon, 29 Jun 2020 23:35:35: 27000000 INFO @ Mon, 29 Jun 2020 23:35:38: 32000000 INFO @ Mon, 29 Jun 2020 23:35:40: 28000000 INFO @ Mon, 29 Jun 2020 23:35:43: 33000000 INFO @ Mon, 29 Jun 2020 23:35:45: 29000000 INFO @ Mon, 29 Jun 2020 23:35:46: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:35:46: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:35:46: #1 total tags in treatment: 33607976 INFO @ Mon, 29 Jun 2020 23:35:46: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:35:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:35:46: #1 tags after filtering in treatment: 33607976 INFO @ Mon, 29 Jun 2020 23:35:46: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:35:46: #1 finished! INFO @ Mon, 29 Jun 2020 23:35:46: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:35:46: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:35:49: #2 number of paired peaks: 39 WARNING @ Mon, 29 Jun 2020 23:35:49: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:35:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981617/SRX3981617.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981617/SRX3981617.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981617/SRX3981617.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981617/SRX3981617.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:35:50: 30000000 INFO @ Mon, 29 Jun 2020 23:35:54: 31000000 INFO @ Mon, 29 Jun 2020 23:35:59: 32000000 INFO @ Mon, 29 Jun 2020 23:36:04: 33000000 INFO @ Mon, 29 Jun 2020 23:36:07: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:36:07: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:36:07: #1 total tags in treatment: 33607976 INFO @ Mon, 29 Jun 2020 23:36:07: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:36:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:36:08: #1 tags after filtering in treatment: 33607976 INFO @ Mon, 29 Jun 2020 23:36:08: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:36:08: #1 finished! INFO @ Mon, 29 Jun 2020 23:36:08: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:36:08: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:36:10: #2 number of paired peaks: 39 WARNING @ Mon, 29 Jun 2020 23:36:10: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:36:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981617/SRX3981617.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981617/SRX3981617.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981617/SRX3981617.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981617/SRX3981617.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。