Job ID = 6528013 SRX = SRX3981610 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:16:35 prefetch.2.10.7: 1) Downloading 'SRR7050214'... 2020-06-29T14:16:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:17:19 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:17:20 prefetch.2.10.7: 'SRR7050214' is valid 2020-06-29T14:17:20 prefetch.2.10.7: 1) 'SRR7050214' was downloaded successfully 2020-06-29T14:17:20 prefetch.2.10.7: 'SRR7050214' has 0 unresolved dependencies Read 14950231 spots for SRR7050214/SRR7050214.sra Written 14950231 spots for SRR7050214/SRR7050214.sra 2020-06-29T14:18:19 prefetch.2.10.7: 1) Downloading 'SRR7050215'... 2020-06-29T14:18:19 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:19:16 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:19:17 prefetch.2.10.7: 'SRR7050215' is valid 2020-06-29T14:19:17 prefetch.2.10.7: 1) 'SRR7050215' was downloaded successfully 2020-06-29T14:19:17 prefetch.2.10.7: 'SRR7050215' has 0 unresolved dependencies Read 14659531 spots for SRR7050215/SRR7050215.sra Written 14659531 spots for SRR7050215/SRR7050215.sra 2020-06-29T14:20:16 prefetch.2.10.7: 1) Downloading 'SRR7050216'... 2020-06-29T14:20:16 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:21:16 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:21:16 prefetch.2.10.7: 'SRR7050216' is valid 2020-06-29T14:21:16 prefetch.2.10.7: 1) 'SRR7050216' was downloaded successfully 2020-06-29T14:21:16 prefetch.2.10.7: 'SRR7050216' has 0 unresolved dependencies Read 14916405 spots for SRR7050216/SRR7050216.sra Written 14916405 spots for SRR7050216/SRR7050216.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:28 44526167 reads; of these: 44526167 (100.00%) were unpaired; of these: 1305860 (2.93%) aligned 0 times 31818293 (71.46%) aligned exactly 1 time 11402014 (25.61%) aligned >1 times 97.07% overall alignment rate Time searching: 00:13:28 Overall time: 00:13:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 5110357 / 43220307 = 0.1182 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:54:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981610/SRX3981610.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981610/SRX3981610.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981610/SRX3981610.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981610/SRX3981610.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:54:38: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:54:38: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:54:43: 1000000 INFO @ Mon, 29 Jun 2020 23:54:49: 2000000 INFO @ Mon, 29 Jun 2020 23:54:54: 3000000 INFO @ Mon, 29 Jun 2020 23:54:59: 4000000 INFO @ Mon, 29 Jun 2020 23:55:04: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:55:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981610/SRX3981610.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981610/SRX3981610.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981610/SRX3981610.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981610/SRX3981610.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:55:08: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:55:08: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:55:09: 6000000 INFO @ Mon, 29 Jun 2020 23:55:15: 7000000 INFO @ Mon, 29 Jun 2020 23:55:15: 1000000 INFO @ Mon, 29 Jun 2020 23:55:20: 8000000 INFO @ Mon, 29 Jun 2020 23:55:21: 2000000 INFO @ Mon, 29 Jun 2020 23:55:26: 9000000 INFO @ Mon, 29 Jun 2020 23:55:27: 3000000 INFO @ Mon, 29 Jun 2020 23:55:32: 10000000 INFO @ Mon, 29 Jun 2020 23:55:34: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:55:38: 11000000 INFO @ Mon, 29 Jun 2020 23:55:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3981610/SRX3981610.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3981610/SRX3981610.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3981610/SRX3981610.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3981610/SRX3981610.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:55:38: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:55:38: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:55:40: 5000000 INFO @ Mon, 29 Jun 2020 23:55:44: 12000000 INFO @ Mon, 29 Jun 2020 23:55:44: 1000000 INFO @ Mon, 29 Jun 2020 23:55:47: 6000000 INFO @ Mon, 29 Jun 2020 23:55:49: 13000000 INFO @ Mon, 29 Jun 2020 23:55:50: 2000000 INFO @ Mon, 29 Jun 2020 23:55:53: 7000000 INFO @ Mon, 29 Jun 2020 23:55:55: 14000000 INFO @ Mon, 29 Jun 2020 23:55:56: 3000000 INFO @ Mon, 29 Jun 2020 23:56:00: 8000000 INFO @ Mon, 29 Jun 2020 23:56:01: 15000000 INFO @ Mon, 29 Jun 2020 23:56:02: 4000000 INFO @ Mon, 29 Jun 2020 23:56:06: 9000000 INFO @ Mon, 29 Jun 2020 23:56:07: 16000000 INFO @ Mon, 29 Jun 2020 23:56:07: 5000000 INFO @ Mon, 29 Jun 2020 23:56:12: 17000000 INFO @ Mon, 29 Jun 2020 23:56:13: 10000000 INFO @ Mon, 29 Jun 2020 23:56:13: 6000000 INFO @ Mon, 29 Jun 2020 23:56:18: 18000000 INFO @ Mon, 29 Jun 2020 23:56:19: 7000000 INFO @ Mon, 29 Jun 2020 23:56:19: 11000000 INFO @ Mon, 29 Jun 2020 23:56:24: 19000000 INFO @ Mon, 29 Jun 2020 23:56:25: 8000000 INFO @ Mon, 29 Jun 2020 23:56:26: 12000000 INFO @ Mon, 29 Jun 2020 23:56:30: 20000000 INFO @ Mon, 29 Jun 2020 23:56:31: 9000000 INFO @ Mon, 29 Jun 2020 23:56:32: 13000000 INFO @ Mon, 29 Jun 2020 23:56:36: 21000000 INFO @ Mon, 29 Jun 2020 23:56:37: 10000000 INFO @ Mon, 29 Jun 2020 23:56:38: 14000000 INFO @ Mon, 29 Jun 2020 23:56:42: 22000000 INFO @ Mon, 29 Jun 2020 23:56:43: 11000000 INFO @ Mon, 29 Jun 2020 23:56:45: 15000000 INFO @ Mon, 29 Jun 2020 23:56:47: 23000000 INFO @ Mon, 29 Jun 2020 23:56:48: 12000000 INFO @ Mon, 29 Jun 2020 23:56:51: 16000000 INFO @ Mon, 29 Jun 2020 23:56:53: 24000000 INFO @ Mon, 29 Jun 2020 23:56:54: 13000000 INFO @ Mon, 29 Jun 2020 23:56:58: 17000000 INFO @ Mon, 29 Jun 2020 23:56:59: 25000000 INFO @ Mon, 29 Jun 2020 23:57:00: 14000000 INFO @ Mon, 29 Jun 2020 23:57:05: 18000000 INFO @ Mon, 29 Jun 2020 23:57:05: 26000000 INFO @ Mon, 29 Jun 2020 23:57:06: 15000000 INFO @ Mon, 29 Jun 2020 23:57:11: 27000000 INFO @ Mon, 29 Jun 2020 23:57:11: 19000000 INFO @ Mon, 29 Jun 2020 23:57:12: 16000000 INFO @ Mon, 29 Jun 2020 23:57:17: 28000000 INFO @ Mon, 29 Jun 2020 23:57:17: 20000000 INFO @ Mon, 29 Jun 2020 23:57:18: 17000000 INFO @ Mon, 29 Jun 2020 23:57:23: 29000000 INFO @ Mon, 29 Jun 2020 23:57:24: 18000000 INFO @ Mon, 29 Jun 2020 23:57:24: 21000000 INFO @ Mon, 29 Jun 2020 23:57:29: 30000000 INFO @ Mon, 29 Jun 2020 23:57:30: 19000000 INFO @ Mon, 29 Jun 2020 23:57:30: 22000000 INFO @ Mon, 29 Jun 2020 23:57:34: 31000000 INFO @ Mon, 29 Jun 2020 23:57:35: 20000000 INFO @ Mon, 29 Jun 2020 23:57:37: 23000000 INFO @ Mon, 29 Jun 2020 23:57:40: 32000000 INFO @ Mon, 29 Jun 2020 23:57:41: 21000000 INFO @ Mon, 29 Jun 2020 23:57:43: 24000000 INFO @ Mon, 29 Jun 2020 23:57:46: 33000000 INFO @ Mon, 29 Jun 2020 23:57:47: 22000000 INFO @ Mon, 29 Jun 2020 23:57:50: 25000000 INFO @ Mon, 29 Jun 2020 23:57:52: 34000000 INFO @ Mon, 29 Jun 2020 23:57:53: 23000000 INFO @ Mon, 29 Jun 2020 23:57:56: 26000000 INFO @ Mon, 29 Jun 2020 23:57:58: 35000000 INFO @ Mon, 29 Jun 2020 23:57:59: 24000000 INFO @ Mon, 29 Jun 2020 23:58:03: 27000000 INFO @ Mon, 29 Jun 2020 23:58:04: 36000000 INFO @ Mon, 29 Jun 2020 23:58:05: 25000000 INFO @ Mon, 29 Jun 2020 23:58:09: 28000000 INFO @ Mon, 29 Jun 2020 23:58:10: 37000000 INFO @ Mon, 29 Jun 2020 23:58:11: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:58:16: 29000000 INFO @ Mon, 29 Jun 2020 23:58:16: 38000000 INFO @ Mon, 29 Jun 2020 23:58:17: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:58:17: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:58:17: #1 total tags in treatment: 38109950 INFO @ Mon, 29 Jun 2020 23:58:17: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:58:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:58:17: 27000000 INFO @ Mon, 29 Jun 2020 23:58:18: #1 tags after filtering in treatment: 38109950 INFO @ Mon, 29 Jun 2020 23:58:18: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:58:18: #1 finished! INFO @ Mon, 29 Jun 2020 23:58:18: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:58:18: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:58:20: #2 number of paired peaks: 9 WARNING @ Mon, 29 Jun 2020 23:58:20: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:58:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981610/SRX3981610.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981610/SRX3981610.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981610/SRX3981610.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981610/SRX3981610.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:58:22: 30000000 INFO @ Mon, 29 Jun 2020 23:58:23: 28000000 INFO @ Mon, 29 Jun 2020 23:58:29: 31000000 INFO @ Mon, 29 Jun 2020 23:58:29: 29000000 INFO @ Mon, 29 Jun 2020 23:58:35: 30000000 INFO @ Mon, 29 Jun 2020 23:58:35: 32000000 INFO @ Mon, 29 Jun 2020 23:58:40: 31000000 INFO @ Mon, 29 Jun 2020 23:58:41: 33000000 INFO @ Mon, 29 Jun 2020 23:58:46: 32000000 INFO @ Mon, 29 Jun 2020 23:58:48: 34000000 INFO @ Mon, 29 Jun 2020 23:58:52: 33000000 INFO @ Mon, 29 Jun 2020 23:58:55: 35000000 INFO @ Mon, 29 Jun 2020 23:58:58: 34000000 INFO @ Mon, 29 Jun 2020 23:59:02: 36000000 INFO @ Mon, 29 Jun 2020 23:59:04: 35000000 INFO @ Mon, 29 Jun 2020 23:59:08: 37000000 INFO @ Mon, 29 Jun 2020 23:59:10: 36000000 INFO @ Mon, 29 Jun 2020 23:59:15: 38000000 INFO @ Mon, 29 Jun 2020 23:59:15: 37000000 INFO @ Mon, 29 Jun 2020 23:59:16: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:59:16: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:59:16: #1 total tags in treatment: 38109950 INFO @ Mon, 29 Jun 2020 23:59:16: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:59:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:59:16: #1 tags after filtering in treatment: 38109950 INFO @ Mon, 29 Jun 2020 23:59:16: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:59:16: #1 finished! INFO @ Mon, 29 Jun 2020 23:59:16: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:59:16: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:59:19: #2 number of paired peaks: 9 WARNING @ Mon, 29 Jun 2020 23:59:19: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:59:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981610/SRX3981610.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981610/SRX3981610.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981610/SRX3981610.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981610/SRX3981610.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:59:21: 38000000 INFO @ Mon, 29 Jun 2020 23:59:22: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:59:22: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:59:22: #1 total tags in treatment: 38109950 INFO @ Mon, 29 Jun 2020 23:59:22: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:59:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:59:22: #1 tags after filtering in treatment: 38109950 INFO @ Mon, 29 Jun 2020 23:59:22: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:59:22: #1 finished! INFO @ Mon, 29 Jun 2020 23:59:22: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:59:22: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:59:25: #2 number of paired peaks: 9 WARNING @ Mon, 29 Jun 2020 23:59:25: Too few paired peaks (9) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:59:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3981610/SRX3981610.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981610/SRX3981610.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981610/SRX3981610.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3981610/SRX3981610.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。