Job ID = 12265208 SRX = SRX3945498 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 47514313 spots for SRR7013048/SRR7013048.sra Written 47514313 spots for SRR7013048/SRR7013048.sra fastq に変換しました。 bowtie でマッピング中... Your job 12265591 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:57:18 47514313 reads; of these: 47514313 (100.00%) were paired; of these: 10739673 (22.60%) aligned concordantly 0 times 28219201 (59.39%) aligned concordantly exactly 1 time 8555439 (18.01%) aligned concordantly >1 times ---- 10739673 pairs aligned concordantly 0 times; of these: 3296069 (30.69%) aligned discordantly 1 time ---- 7443604 pairs aligned 0 times concordantly or discordantly; of these: 14887208 mates make up the pairs; of these: 12828114 (86.17%) aligned 0 times 930182 (6.25%) aligned exactly 1 time 1128912 (7.58%) aligned >1 times 86.50% overall alignment rate Time searching: 00:57:18 Overall time: 00:57:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 36 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 11456307 / 34750932 = 0.3297 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:37:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3945498/SRX3945498.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3945498/SRX3945498.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3945498/SRX3945498.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3945498/SRX3945498.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:37:34: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:37:34: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:37:40: 1000000 INFO @ Sat, 03 Apr 2021 07:37:46: 2000000 INFO @ Sat, 03 Apr 2021 07:37:53: 3000000 INFO @ Sat, 03 Apr 2021 07:37:59: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:38:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3945498/SRX3945498.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3945498/SRX3945498.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3945498/SRX3945498.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3945498/SRX3945498.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:38:04: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:38:04: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:38:05: 5000000 INFO @ Sat, 03 Apr 2021 07:38:11: 1000000 INFO @ Sat, 03 Apr 2021 07:38:12: 6000000 INFO @ Sat, 03 Apr 2021 07:38:18: 2000000 INFO @ Sat, 03 Apr 2021 07:38:19: 7000000 INFO @ Sat, 03 Apr 2021 07:38:25: 3000000 INFO @ Sat, 03 Apr 2021 07:38:26: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 03 Apr 2021 07:38:32: 4000000 INFO @ Sat, 03 Apr 2021 07:38:33: 9000000 INFO @ Sat, 03 Apr 2021 07:38:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3945498/SRX3945498.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3945498/SRX3945498.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3945498/SRX3945498.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3945498/SRX3945498.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 03 Apr 2021 07:38:34: #1 read tag files... INFO @ Sat, 03 Apr 2021 07:38:34: #1 read treatment tags... INFO @ Sat, 03 Apr 2021 07:38:40: 5000000 INFO @ Sat, 03 Apr 2021 07:38:41: 10000000 INFO @ Sat, 03 Apr 2021 07:38:42: 1000000 INFO @ Sat, 03 Apr 2021 07:38:47: 6000000 INFO @ Sat, 03 Apr 2021 07:38:48: 11000000 INFO @ Sat, 03 Apr 2021 07:38:49: 2000000 INFO @ Sat, 03 Apr 2021 07:38:54: 7000000 INFO @ Sat, 03 Apr 2021 07:38:55: 12000000 INFO @ Sat, 03 Apr 2021 07:38:56: 3000000 INFO @ Sat, 03 Apr 2021 07:39:01: 8000000 INFO @ Sat, 03 Apr 2021 07:39:03: 13000000 INFO @ Sat, 03 Apr 2021 07:39:04: 4000000 INFO @ Sat, 03 Apr 2021 07:39:09: 9000000 INFO @ Sat, 03 Apr 2021 07:39:11: 14000000 INFO @ Sat, 03 Apr 2021 07:39:12: 5000000 INFO @ Sat, 03 Apr 2021 07:39:17: 10000000 INFO @ Sat, 03 Apr 2021 07:39:19: 15000000 INFO @ Sat, 03 Apr 2021 07:39:20: 6000000 INFO @ Sat, 03 Apr 2021 07:39:24: 11000000 INFO @ Sat, 03 Apr 2021 07:39:27: 16000000 INFO @ Sat, 03 Apr 2021 07:39:28: 7000000 INFO @ Sat, 03 Apr 2021 07:39:31: 12000000 INFO @ Sat, 03 Apr 2021 07:39:35: 17000000 INFO @ Sat, 03 Apr 2021 07:39:36: 8000000 INFO @ Sat, 03 Apr 2021 07:39:39: 13000000 INFO @ Sat, 03 Apr 2021 07:39:42: 18000000 INFO @ Sat, 03 Apr 2021 07:39:43: 9000000 INFO @ Sat, 03 Apr 2021 07:39:46: 14000000 INFO @ Sat, 03 Apr 2021 07:39:50: 19000000 INFO @ Sat, 03 Apr 2021 07:39:51: 10000000 INFO @ Sat, 03 Apr 2021 07:39:54: 15000000 INFO @ Sat, 03 Apr 2021 07:39:58: 20000000 INFO @ Sat, 03 Apr 2021 07:39:59: 11000000 INFO @ Sat, 03 Apr 2021 07:40:01: 16000000 INFO @ Sat, 03 Apr 2021 07:40:05: 21000000 INFO @ Sat, 03 Apr 2021 07:40:06: 12000000 INFO @ Sat, 03 Apr 2021 07:40:08: 17000000 INFO @ Sat, 03 Apr 2021 07:40:13: 22000000 INFO @ Sat, 03 Apr 2021 07:40:14: 13000000 INFO @ Sat, 03 Apr 2021 07:40:15: 18000000 INFO @ Sat, 03 Apr 2021 07:40:21: 23000000 INFO @ Sat, 03 Apr 2021 07:40:22: 14000000 INFO @ Sat, 03 Apr 2021 07:40:22: 19000000 INFO @ Sat, 03 Apr 2021 07:40:28: 24000000 INFO @ Sat, 03 Apr 2021 07:40:29: 15000000 INFO @ Sat, 03 Apr 2021 07:40:30: 20000000 INFO @ Sat, 03 Apr 2021 07:40:36: 25000000 INFO @ Sat, 03 Apr 2021 07:40:37: 21000000 INFO @ Sat, 03 Apr 2021 07:40:37: 16000000 INFO @ Sat, 03 Apr 2021 07:40:44: 26000000 INFO @ Sat, 03 Apr 2021 07:40:44: 22000000 INFO @ Sat, 03 Apr 2021 07:40:44: 17000000 INFO @ Sat, 03 Apr 2021 07:40:51: 27000000 INFO @ Sat, 03 Apr 2021 07:40:51: 23000000 INFO @ Sat, 03 Apr 2021 07:40:52: 18000000 INFO @ Sat, 03 Apr 2021 07:40:58: 28000000 INFO @ Sat, 03 Apr 2021 07:40:59: 24000000 INFO @ Sat, 03 Apr 2021 07:40:59: 19000000 INFO @ Sat, 03 Apr 2021 07:41:06: 25000000 INFO @ Sat, 03 Apr 2021 07:41:06: 29000000 INFO @ Sat, 03 Apr 2021 07:41:07: 20000000 INFO @ Sat, 03 Apr 2021 07:41:13: 26000000 INFO @ Sat, 03 Apr 2021 07:41:14: 30000000 INFO @ Sat, 03 Apr 2021 07:41:15: 21000000 INFO @ Sat, 03 Apr 2021 07:41:20: 27000000 INFO @ Sat, 03 Apr 2021 07:41:22: 31000000 INFO @ Sat, 03 Apr 2021 07:41:23: 22000000 INFO @ Sat, 03 Apr 2021 07:41:28: 28000000 INFO @ Sat, 03 Apr 2021 07:41:29: 32000000 INFO @ Sat, 03 Apr 2021 07:41:30: 23000000 INFO @ Sat, 03 Apr 2021 07:41:35: 29000000 INFO @ Sat, 03 Apr 2021 07:41:37: 33000000 INFO @ Sat, 03 Apr 2021 07:41:38: 24000000 INFO @ Sat, 03 Apr 2021 07:41:42: 30000000 INFO @ Sat, 03 Apr 2021 07:41:45: 34000000 INFO @ Sat, 03 Apr 2021 07:41:46: 25000000 INFO @ Sat, 03 Apr 2021 07:41:49: 31000000 INFO @ Sat, 03 Apr 2021 07:41:53: 35000000 INFO @ Sat, 03 Apr 2021 07:41:54: 26000000 INFO @ Sat, 03 Apr 2021 07:41:56: 32000000 INFO @ Sat, 03 Apr 2021 07:42:00: 36000000 INFO @ Sat, 03 Apr 2021 07:42:01: 27000000 INFO @ Sat, 03 Apr 2021 07:42:04: 33000000 INFO @ Sat, 03 Apr 2021 07:42:07: 37000000 INFO @ Sat, 03 Apr 2021 07:42:09: 28000000 INFO @ Sat, 03 Apr 2021 07:42:11: 34000000 INFO @ Sat, 03 Apr 2021 07:42:15: 38000000 INFO @ Sat, 03 Apr 2021 07:42:16: 29000000 INFO @ Sat, 03 Apr 2021 07:42:18: 35000000 INFO @ Sat, 03 Apr 2021 07:42:23: 39000000 INFO @ Sat, 03 Apr 2021 07:42:24: 30000000 INFO @ Sat, 03 Apr 2021 07:42:25: 36000000 INFO @ Sat, 03 Apr 2021 07:42:30: 40000000 INFO @ Sat, 03 Apr 2021 07:42:31: 31000000 INFO @ Sat, 03 Apr 2021 07:42:32: 37000000 INFO @ Sat, 03 Apr 2021 07:42:37: 41000000 INFO @ Sat, 03 Apr 2021 07:42:39: 32000000 INFO @ Sat, 03 Apr 2021 07:42:39: 38000000 INFO @ Sat, 03 Apr 2021 07:42:45: 42000000 INFO @ Sat, 03 Apr 2021 07:42:46: 33000000 INFO @ Sat, 03 Apr 2021 07:42:47: 39000000 INFO @ Sat, 03 Apr 2021 07:42:53: 43000000 INFO @ Sat, 03 Apr 2021 07:42:54: 34000000 INFO @ Sat, 03 Apr 2021 07:42:54: 40000000 INFO @ Sat, 03 Apr 2021 07:43:00: 44000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 03 Apr 2021 07:43:01: 41000000 INFO @ Sat, 03 Apr 2021 07:43:01: 35000000 INFO @ Sat, 03 Apr 2021 07:43:08: 45000000 INFO @ Sat, 03 Apr 2021 07:43:09: 42000000 INFO @ Sat, 03 Apr 2021 07:43:09: 36000000 INFO @ Sat, 03 Apr 2021 07:43:16: 46000000 INFO @ Sat, 03 Apr 2021 07:43:16: 43000000 INFO @ Sat, 03 Apr 2021 07:43:17: 37000000 INFO @ Sat, 03 Apr 2021 07:43:23: 44000000 INFO @ Sat, 03 Apr 2021 07:43:24: 47000000 INFO @ Sat, 03 Apr 2021 07:43:25: 38000000 INFO @ Sat, 03 Apr 2021 07:43:31: 45000000 INFO @ Sat, 03 Apr 2021 07:43:32: 48000000 INFO @ Sat, 03 Apr 2021 07:43:32: 39000000 INFO @ Sat, 03 Apr 2021 07:43:38: 46000000 INFO @ Sat, 03 Apr 2021 07:43:40: 49000000 INFO @ Sat, 03 Apr 2021 07:43:40: 40000000 INFO @ Sat, 03 Apr 2021 07:43:45: 47000000 INFO @ Sat, 03 Apr 2021 07:43:48: 50000000 INFO @ Sat, 03 Apr 2021 07:43:48: 41000000 INFO @ Sat, 03 Apr 2021 07:43:53: 48000000 INFO @ Sat, 03 Apr 2021 07:43:55: 42000000 INFO @ Sat, 03 Apr 2021 07:43:55: 51000000 INFO @ Sat, 03 Apr 2021 07:44:00: 49000000 INFO @ Sat, 03 Apr 2021 07:44:03: 43000000 INFO @ Sat, 03 Apr 2021 07:44:03: 52000000 INFO @ Sat, 03 Apr 2021 07:44:07: 50000000 INFO @ Sat, 03 Apr 2021 07:44:10: 44000000 INFO @ Sat, 03 Apr 2021 07:44:11: 53000000 INFO @ Sat, 03 Apr 2021 07:44:15: 51000000 INFO @ Sat, 03 Apr 2021 07:44:18: 45000000 INFO @ Sat, 03 Apr 2021 07:44:18: 54000000 BigWig に変換しました。 INFO @ Sat, 03 Apr 2021 07:44:22: 52000000 INFO @ Sat, 03 Apr 2021 07:44:25: 55000000 INFO @ Sat, 03 Apr 2021 07:44:26: 46000000 INFO @ Sat, 03 Apr 2021 07:44:30: 53000000 INFO @ Sat, 03 Apr 2021 07:44:33: 56000000 INFO @ Sat, 03 Apr 2021 07:44:33: 47000000 INFO @ Sat, 03 Apr 2021 07:44:37: 54000000 INFO @ Sat, 03 Apr 2021 07:44:40: 57000000 INFO @ Sat, 03 Apr 2021 07:44:41: 48000000 INFO @ Sat, 03 Apr 2021 07:44:44: 55000000 INFO @ Sat, 03 Apr 2021 07:44:48: 58000000 INFO @ Sat, 03 Apr 2021 07:44:49: 49000000 INFO @ Sat, 03 Apr 2021 07:44:51: 56000000 INFO @ Sat, 03 Apr 2021 07:44:56: 59000000 INFO @ Sat, 03 Apr 2021 07:44:57: 50000000 INFO @ Sat, 03 Apr 2021 07:44:58: 57000000 INFO @ Sat, 03 Apr 2021 07:44:59: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:44:59: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:44:59: #1 total tags in treatment: 25876996 INFO @ Sat, 03 Apr 2021 07:44:59: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:44:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:44:59: #1 tags after filtering in treatment: 16775596 INFO @ Sat, 03 Apr 2021 07:44:59: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 03 Apr 2021 07:44:59: #1 finished! INFO @ Sat, 03 Apr 2021 07:44:59: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:44:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:45:00: #2 number of paired peaks: 444 WARNING @ Sat, 03 Apr 2021 07:45:00: Fewer paired peaks (444) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 444 pairs to build model! INFO @ Sat, 03 Apr 2021 07:45:00: start model_add_line... INFO @ Sat, 03 Apr 2021 07:45:01: start X-correlation... INFO @ Sat, 03 Apr 2021 07:45:01: end of X-cor INFO @ Sat, 03 Apr 2021 07:45:01: #2 finished! INFO @ Sat, 03 Apr 2021 07:45:01: #2 predicted fragment length is 40 bps INFO @ Sat, 03 Apr 2021 07:45:01: #2 alternative fragment length(s) may be 4,40 bps INFO @ Sat, 03 Apr 2021 07:45:01: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX3945498/SRX3945498.05_model.r WARNING @ Sat, 03 Apr 2021 07:45:01: #2 Since the d (40) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:45:01: #2 You may need to consider one of the other alternative d(s): 4,40 WARNING @ Sat, 03 Apr 2021 07:45:01: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:45:01: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:45:01: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:45:04: 51000000 INFO @ Sat, 03 Apr 2021 07:45:05: 58000000 INFO @ Sat, 03 Apr 2021 07:45:12: 52000000 INFO @ Sat, 03 Apr 2021 07:45:13: 59000000 INFO @ Sat, 03 Apr 2021 07:45:15: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:45:15: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:45:15: #1 total tags in treatment: 25876996 INFO @ Sat, 03 Apr 2021 07:45:15: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:45:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:45:15: #1 tags after filtering in treatment: 16775596 INFO @ Sat, 03 Apr 2021 07:45:15: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 03 Apr 2021 07:45:15: #1 finished! INFO @ Sat, 03 Apr 2021 07:45:15: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:45:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:45:17: #2 number of paired peaks: 444 WARNING @ Sat, 03 Apr 2021 07:45:17: Fewer paired peaks (444) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 444 pairs to build model! INFO @ Sat, 03 Apr 2021 07:45:17: start model_add_line... INFO @ Sat, 03 Apr 2021 07:45:17: start X-correlation... INFO @ Sat, 03 Apr 2021 07:45:17: end of X-cor INFO @ Sat, 03 Apr 2021 07:45:17: #2 finished! INFO @ Sat, 03 Apr 2021 07:45:17: #2 predicted fragment length is 40 bps INFO @ Sat, 03 Apr 2021 07:45:17: #2 alternative fragment length(s) may be 4,40 bps INFO @ Sat, 03 Apr 2021 07:45:17: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX3945498/SRX3945498.10_model.r WARNING @ Sat, 03 Apr 2021 07:45:17: #2 Since the d (40) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:45:17: #2 You may need to consider one of the other alternative d(s): 4,40 WARNING @ Sat, 03 Apr 2021 07:45:17: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:45:17: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:45:17: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 03 Apr 2021 07:45:18: 53000000 INFO @ Sat, 03 Apr 2021 07:45:24: 54000000 INFO @ Sat, 03 Apr 2021 07:45:31: 55000000 INFO @ Sat, 03 Apr 2021 07:45:37: 56000000 INFO @ Sat, 03 Apr 2021 07:45:40: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:45:43: 57000000 INFO @ Sat, 03 Apr 2021 07:45:50: 58000000 INFO @ Sat, 03 Apr 2021 07:45:56: 59000000 INFO @ Sat, 03 Apr 2021 07:45:56: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:45:58: #1 tag size is determined as 50 bps INFO @ Sat, 03 Apr 2021 07:45:58: #1 tag size = 50 INFO @ Sat, 03 Apr 2021 07:45:58: #1 total tags in treatment: 25876996 INFO @ Sat, 03 Apr 2021 07:45:58: #1 user defined the maximum tags... INFO @ Sat, 03 Apr 2021 07:45:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 03 Apr 2021 07:45:58: #1 tags after filtering in treatment: 16775596 INFO @ Sat, 03 Apr 2021 07:45:58: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 03 Apr 2021 07:45:58: #1 finished! INFO @ Sat, 03 Apr 2021 07:45:58: #2 Build Peak Model... INFO @ Sat, 03 Apr 2021 07:45:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 03 Apr 2021 07:45:59: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX3945498/SRX3945498.05_peaks.xls INFO @ Sat, 03 Apr 2021 07:45:59: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX3945498/SRX3945498.05_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:46:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX3945498/SRX3945498.05_summits.bed INFO @ Sat, 03 Apr 2021 07:46:00: Done! INFO @ Sat, 03 Apr 2021 07:46:00: #2 number of paired peaks: 444 WARNING @ Sat, 03 Apr 2021 07:46:00: Fewer paired peaks (444) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 444 pairs to build model! INFO @ Sat, 03 Apr 2021 07:46:00: start model_add_line... INFO @ Sat, 03 Apr 2021 07:46:00: start X-correlation... INFO @ Sat, 03 Apr 2021 07:46:00: end of X-cor INFO @ Sat, 03 Apr 2021 07:46:00: #2 finished! INFO @ Sat, 03 Apr 2021 07:46:00: #2 predicted fragment length is 40 bps INFO @ Sat, 03 Apr 2021 07:46:00: #2 alternative fragment length(s) may be 4,40 bps INFO @ Sat, 03 Apr 2021 07:46:00: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX3945498/SRX3945498.20_model.r WARNING @ Sat, 03 Apr 2021 07:46:00: #2 Since the d (40) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 03 Apr 2021 07:46:00: #2 You may need to consider one of the other alternative d(s): 4,40 WARNING @ Sat, 03 Apr 2021 07:46:00: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 03 Apr 2021 07:46:00: #3 Call peaks... INFO @ Sat, 03 Apr 2021 07:46:00: #3 Pre-compute pvalue-qvalue table... pass1 - making usageList (15 chroms): 5 millis pass2 - checking and writing primary data (19041 records, 4 fields): 21 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:46:16: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX3945498/SRX3945498.10_peaks.xls INFO @ Sat, 03 Apr 2021 07:46:16: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX3945498/SRX3945498.10_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:46:16: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX3945498/SRX3945498.10_summits.bed INFO @ Sat, 03 Apr 2021 07:46:16: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (7860 records, 4 fields): 10 millis CompletedMACS2peakCalling INFO @ Sat, 03 Apr 2021 07:46:38: #3 Call peaks for each chromosome... INFO @ Sat, 03 Apr 2021 07:46:58: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX3945498/SRX3945498.20_peaks.xls INFO @ Sat, 03 Apr 2021 07:46:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX3945498/SRX3945498.20_peaks.narrowPeak INFO @ Sat, 03 Apr 2021 07:46:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX3945498/SRX3945498.20_summits.bed INFO @ Sat, 03 Apr 2021 07:46:58: Done! pass1 - making usageList (10 chroms): 0 millis pass2 - checking and writing primary data (343 records, 4 fields): 2 millis CompletedMACS2peakCalling