Job ID = 6528002 SRX = SRX3723039 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:26:21 prefetch.2.10.7: 1) Downloading 'SRR6750400'... 2020-06-29T14:26:21 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:28:12 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:28:13 prefetch.2.10.7: 'SRR6750400' is valid 2020-06-29T14:28:13 prefetch.2.10.7: 1) 'SRR6750400' was downloaded successfully 2020-06-29T14:28:13 prefetch.2.10.7: 'SRR6750400' has 0 unresolved dependencies Read 11012284 spots for SRR6750400/SRR6750400.sra Written 11012284 spots for SRR6750400/SRR6750400.sra 2020-06-29T14:29:14 prefetch.2.10.7: 1) Downloading 'SRR6750401'... 2020-06-29T14:29:15 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:31:21 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:31:22 prefetch.2.10.7: 'SRR6750401' is valid 2020-06-29T14:31:22 prefetch.2.10.7: 1) 'SRR6750401' was downloaded successfully 2020-06-29T14:31:22 prefetch.2.10.7: 'SRR6750401' has 0 unresolved dependencies Read 10943777 spots for SRR6750401/SRR6750401.sra Written 10943777 spots for SRR6750401/SRR6750401.sra 2020-06-29T14:32:22 prefetch.2.10.7: 1) Downloading 'SRR6750402'... 2020-06-29T14:32:22 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:34:37 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:34:38 prefetch.2.10.7: 'SRR6750402' is valid 2020-06-29T14:34:38 prefetch.2.10.7: 1) 'SRR6750402' was downloaded successfully 2020-06-29T14:34:38 prefetch.2.10.7: 'SRR6750402' has 0 unresolved dependencies Read 11515555 spots for SRR6750402/SRR6750402.sra Written 11515555 spots for SRR6750402/SRR6750402.sra 2020-06-29T14:35:41 prefetch.2.10.7: 1) Downloading 'SRR6750403'... 2020-06-29T14:35:41 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:37:27 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:37:28 prefetch.2.10.7: 'SRR6750403' is valid 2020-06-29T14:37:28 prefetch.2.10.7: 1) 'SRR6750403' was downloaded successfully 2020-06-29T14:37:28 prefetch.2.10.7: 'SRR6750403' has 0 unresolved dependencies Read 11396711 spots for SRR6750403/SRR6750403.sra Written 11396711 spots for SRR6750403/SRR6750403.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:14 44868327 reads; of these: 44868327 (100.00%) were unpaired; of these: 13556141 (30.21%) aligned 0 times 23622876 (52.65%) aligned exactly 1 time 7689310 (17.14%) aligned >1 times 69.79% overall alignment rate Time searching: 00:15:14 Overall time: 00:15:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 10344018 / 31312186 = 0.3304 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:28:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3723039/SRX3723039.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3723039/SRX3723039.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3723039/SRX3723039.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3723039/SRX3723039.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:28:35: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:28:35: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:28:42: 1000000 INFO @ Tue, 30 Jun 2020 00:28:49: 2000000 INFO @ Tue, 30 Jun 2020 00:28:56: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:29:02: 4000000 INFO @ Tue, 30 Jun 2020 00:29:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3723039/SRX3723039.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3723039/SRX3723039.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3723039/SRX3723039.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3723039/SRX3723039.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:29:05: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:29:05: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:29:10: 5000000 INFO @ Tue, 30 Jun 2020 00:29:12: 1000000 INFO @ Tue, 30 Jun 2020 00:29:18: 6000000 INFO @ Tue, 30 Jun 2020 00:29:19: 2000000 INFO @ Tue, 30 Jun 2020 00:29:26: 3000000 INFO @ Tue, 30 Jun 2020 00:29:28: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:29:33: 4000000 INFO @ Tue, 30 Jun 2020 00:29:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3723039/SRX3723039.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3723039/SRX3723039.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3723039/SRX3723039.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3723039/SRX3723039.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:29:35: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:29:35: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:29:37: 8000000 INFO @ Tue, 30 Jun 2020 00:29:40: 5000000 INFO @ Tue, 30 Jun 2020 00:29:43: 1000000 INFO @ Tue, 30 Jun 2020 00:29:44: 9000000 INFO @ Tue, 30 Jun 2020 00:29:48: 6000000 INFO @ Tue, 30 Jun 2020 00:29:52: 2000000 INFO @ Tue, 30 Jun 2020 00:29:52: 10000000 INFO @ Tue, 30 Jun 2020 00:29:56: 7000000 INFO @ Tue, 30 Jun 2020 00:29:59: 11000000 INFO @ Tue, 30 Jun 2020 00:30:00: 3000000 INFO @ Tue, 30 Jun 2020 00:30:03: 8000000 INFO @ Tue, 30 Jun 2020 00:30:07: 12000000 INFO @ Tue, 30 Jun 2020 00:30:08: 4000000 INFO @ Tue, 30 Jun 2020 00:30:11: 9000000 INFO @ Tue, 30 Jun 2020 00:30:14: 13000000 INFO @ Tue, 30 Jun 2020 00:30:17: 5000000 INFO @ Tue, 30 Jun 2020 00:30:18: 10000000 INFO @ Tue, 30 Jun 2020 00:30:22: 14000000 INFO @ Tue, 30 Jun 2020 00:30:25: 6000000 INFO @ Tue, 30 Jun 2020 00:30:26: 11000000 INFO @ Tue, 30 Jun 2020 00:30:29: 15000000 INFO @ Tue, 30 Jun 2020 00:30:33: 12000000 INFO @ Tue, 30 Jun 2020 00:30:33: 7000000 INFO @ Tue, 30 Jun 2020 00:30:37: 16000000 INFO @ Tue, 30 Jun 2020 00:30:40: 13000000 INFO @ Tue, 30 Jun 2020 00:30:41: 8000000 INFO @ Tue, 30 Jun 2020 00:30:44: 17000000 INFO @ Tue, 30 Jun 2020 00:30:48: 14000000 INFO @ Tue, 30 Jun 2020 00:30:49: 9000000 INFO @ Tue, 30 Jun 2020 00:30:51: 18000000 INFO @ Tue, 30 Jun 2020 00:30:55: 15000000 INFO @ Tue, 30 Jun 2020 00:30:57: 10000000 INFO @ Tue, 30 Jun 2020 00:30:59: 19000000 INFO @ Tue, 30 Jun 2020 00:31:03: 16000000 INFO @ Tue, 30 Jun 2020 00:31:05: 11000000 INFO @ Tue, 30 Jun 2020 00:31:07: 20000000 INFO @ Tue, 30 Jun 2020 00:31:11: 17000000 INFO @ Tue, 30 Jun 2020 00:31:13: 12000000 INFO @ Tue, 30 Jun 2020 00:31:14: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 00:31:14: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 00:31:14: #1 total tags in treatment: 20968168 INFO @ Tue, 30 Jun 2020 00:31:14: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:31:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:31:15: #1 tags after filtering in treatment: 20968168 INFO @ Tue, 30 Jun 2020 00:31:15: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:31:15: #1 finished! INFO @ Tue, 30 Jun 2020 00:31:15: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:31:15: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:31:16: #2 number of paired peaks: 23 WARNING @ Tue, 30 Jun 2020 00:31:16: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:31:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3723039/SRX3723039.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3723039/SRX3723039.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3723039/SRX3723039.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3723039/SRX3723039.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:31:18: 18000000 INFO @ Tue, 30 Jun 2020 00:31:21: 13000000 INFO @ Tue, 30 Jun 2020 00:31:25: 19000000 INFO @ Tue, 30 Jun 2020 00:31:29: 14000000 INFO @ Tue, 30 Jun 2020 00:31:32: 20000000 INFO @ Tue, 30 Jun 2020 00:31:37: 15000000 INFO @ Tue, 30 Jun 2020 00:31:38: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 00:31:38: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 00:31:38: #1 total tags in treatment: 20968168 INFO @ Tue, 30 Jun 2020 00:31:38: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:31:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:31:39: #1 tags after filtering in treatment: 20968168 INFO @ Tue, 30 Jun 2020 00:31:39: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:31:39: #1 finished! INFO @ Tue, 30 Jun 2020 00:31:39: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:31:39: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:31:40: #2 number of paired peaks: 23 WARNING @ Tue, 30 Jun 2020 00:31:40: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:31:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3723039/SRX3723039.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3723039/SRX3723039.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3723039/SRX3723039.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3723039/SRX3723039.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:31:44: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:31:53: 17000000 INFO @ Tue, 30 Jun 2020 00:32:01: 18000000 INFO @ Tue, 30 Jun 2020 00:32:09: 19000000 INFO @ Tue, 30 Jun 2020 00:32:17: 20000000 INFO @ Tue, 30 Jun 2020 00:32:25: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 00:32:25: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 00:32:25: #1 total tags in treatment: 20968168 INFO @ Tue, 30 Jun 2020 00:32:25: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:32:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:32:26: #1 tags after filtering in treatment: 20968168 INFO @ Tue, 30 Jun 2020 00:32:26: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 30 Jun 2020 00:32:26: #1 finished! INFO @ Tue, 30 Jun 2020 00:32:26: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:32:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:32:27: #2 number of paired peaks: 23 WARNING @ Tue, 30 Jun 2020 00:32:27: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:32:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3723039/SRX3723039.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3723039/SRX3723039.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3723039/SRX3723039.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3723039/SRX3723039.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。