Job ID = 2590326 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 146,552,705 reads read : 146,552,705 reads written : 146,552,705 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1020541.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:43:34 146552705 reads; of these: 146552705 (100.00%) were unpaired; of these: 42011129 (28.67%) aligned 0 times 96790224 (66.04%) aligned exactly 1 time 7751352 (5.29%) aligned >1 times 71.33% overall alignment rate Time searching: 00:43:34 Overall time: 00:43:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 44 files... [bam_rmdupse_core] 61529495 / 104541576 = 0.5886 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 12 Aug 2019 22:08:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX370362/SRX370362.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX370362/SRX370362.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX370362/SRX370362.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX370362/SRX370362.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:08:16: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:08:16: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:08:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX370362/SRX370362.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX370362/SRX370362.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX370362/SRX370362.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX370362/SRX370362.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:08:17: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:08:17: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:08:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX370362/SRX370362.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX370362/SRX370362.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX370362/SRX370362.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX370362/SRX370362.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 12 Aug 2019 22:08:18: #1 read tag files... INFO @ Mon, 12 Aug 2019 22:08:18: #1 read treatment tags... INFO @ Mon, 12 Aug 2019 22:08:25: 1000000 INFO @ Mon, 12 Aug 2019 22:08:26: 1000000 INFO @ Mon, 12 Aug 2019 22:08:27: 1000000 INFO @ Mon, 12 Aug 2019 22:08:35: 2000000 INFO @ Mon, 12 Aug 2019 22:08:36: 2000000 INFO @ Mon, 12 Aug 2019 22:08:37: 2000000 INFO @ Mon, 12 Aug 2019 22:08:44: 3000000 INFO @ Mon, 12 Aug 2019 22:08:46: 3000000 INFO @ Mon, 12 Aug 2019 22:08:47: 3000000 INFO @ Mon, 12 Aug 2019 22:08:54: 4000000 INFO @ Mon, 12 Aug 2019 22:08:55: 4000000 INFO @ Mon, 12 Aug 2019 22:08:56: 4000000 INFO @ Mon, 12 Aug 2019 22:09:03: 5000000 INFO @ Mon, 12 Aug 2019 22:09:05: 5000000 INFO @ Mon, 12 Aug 2019 22:09:05: 5000000 INFO @ Mon, 12 Aug 2019 22:09:13: 6000000 INFO @ Mon, 12 Aug 2019 22:09:14: 6000000 INFO @ Mon, 12 Aug 2019 22:09:15: 6000000 INFO @ Mon, 12 Aug 2019 22:09:22: 7000000 INFO @ Mon, 12 Aug 2019 22:09:23: 7000000 INFO @ Mon, 12 Aug 2019 22:09:24: 7000000 INFO @ Mon, 12 Aug 2019 22:09:31: 8000000 INFO @ Mon, 12 Aug 2019 22:09:33: 8000000 INFO @ Mon, 12 Aug 2019 22:09:33: 8000000 INFO @ Mon, 12 Aug 2019 22:09:40: 9000000 INFO @ Mon, 12 Aug 2019 22:09:42: 9000000 INFO @ Mon, 12 Aug 2019 22:09:43: 9000000 INFO @ Mon, 12 Aug 2019 22:09:49: 10000000 INFO @ Mon, 12 Aug 2019 22:09:52: 10000000 INFO @ Mon, 12 Aug 2019 22:09:52: 10000000 INFO @ Mon, 12 Aug 2019 22:09:59: 11000000 INFO @ Mon, 12 Aug 2019 22:10:01: 11000000 INFO @ Mon, 12 Aug 2019 22:10:01: 11000000 INFO @ Mon, 12 Aug 2019 22:10:08: 12000000 INFO @ Mon, 12 Aug 2019 22:10:10: 12000000 INFO @ Mon, 12 Aug 2019 22:10:10: 12000000 INFO @ Mon, 12 Aug 2019 22:10:17: 13000000 INFO @ Mon, 12 Aug 2019 22:10:19: 13000000 INFO @ Mon, 12 Aug 2019 22:10:20: 13000000 INFO @ Mon, 12 Aug 2019 22:10:27: 14000000 INFO @ Mon, 12 Aug 2019 22:10:28: 14000000 INFO @ Mon, 12 Aug 2019 22:10:29: 14000000 INFO @ Mon, 12 Aug 2019 22:10:37: 15000000 INFO @ Mon, 12 Aug 2019 22:10:37: 15000000 INFO @ Mon, 12 Aug 2019 22:10:40: 15000000 INFO @ Mon, 12 Aug 2019 22:10:46: 16000000 INFO @ Mon, 12 Aug 2019 22:10:46: 16000000 INFO @ Mon, 12 Aug 2019 22:10:49: 16000000 INFO @ Mon, 12 Aug 2019 22:10:55: 17000000 INFO @ Mon, 12 Aug 2019 22:10:56: 17000000 INFO @ Mon, 12 Aug 2019 22:10:58: 17000000 INFO @ Mon, 12 Aug 2019 22:11:04: 18000000 INFO @ Mon, 12 Aug 2019 22:11:06: 18000000 INFO @ Mon, 12 Aug 2019 22:11:08: 18000000 INFO @ Mon, 12 Aug 2019 22:11:13: 19000000 INFO @ Mon, 12 Aug 2019 22:11:15: 19000000 INFO @ Mon, 12 Aug 2019 22:11:17: 19000000 INFO @ Mon, 12 Aug 2019 22:11:22: 20000000 INFO @ Mon, 12 Aug 2019 22:11:25: 20000000 INFO @ Mon, 12 Aug 2019 22:11:27: 20000000 INFO @ Mon, 12 Aug 2019 22:11:31: 21000000 INFO @ Mon, 12 Aug 2019 22:11:34: 21000000 INFO @ Mon, 12 Aug 2019 22:11:37: 21000000 INFO @ Mon, 12 Aug 2019 22:11:40: 22000000 INFO @ Mon, 12 Aug 2019 22:11:45: 22000000 INFO @ Mon, 12 Aug 2019 22:11:47: 22000000 INFO @ Mon, 12 Aug 2019 22:11:49: 23000000 INFO @ Mon, 12 Aug 2019 22:11:54: 23000000 INFO @ Mon, 12 Aug 2019 22:11:56: 23000000 INFO @ Mon, 12 Aug 2019 22:11:57: 24000000 INFO @ Mon, 12 Aug 2019 22:12:04: 24000000 INFO @ Mon, 12 Aug 2019 22:12:06: 25000000 INFO @ Mon, 12 Aug 2019 22:12:06: 24000000 INFO @ Mon, 12 Aug 2019 22:12:13: 25000000 INFO @ Mon, 12 Aug 2019 22:12:15: 26000000 INFO @ Mon, 12 Aug 2019 22:12:15: 25000000 INFO @ Mon, 12 Aug 2019 22:12:22: 26000000 INFO @ Mon, 12 Aug 2019 22:12:23: 27000000 INFO @ Mon, 12 Aug 2019 22:12:25: 26000000 INFO @ Mon, 12 Aug 2019 22:12:32: 27000000 INFO @ Mon, 12 Aug 2019 22:12:32: 28000000 INFO @ Mon, 12 Aug 2019 22:12:34: 27000000 INFO @ Mon, 12 Aug 2019 22:12:41: 29000000 INFO @ Mon, 12 Aug 2019 22:12:41: 28000000 INFO @ Mon, 12 Aug 2019 22:12:44: 28000000 INFO @ Mon, 12 Aug 2019 22:12:49: 30000000 INFO @ Mon, 12 Aug 2019 22:12:50: 29000000 INFO @ Mon, 12 Aug 2019 22:12:53: 29000000 INFO @ Mon, 12 Aug 2019 22:12:58: 31000000 INFO @ Mon, 12 Aug 2019 22:12:59: 30000000 INFO @ Mon, 12 Aug 2019 22:13:02: 30000000 INFO @ Mon, 12 Aug 2019 22:13:06: 32000000 INFO @ Mon, 12 Aug 2019 22:13:09: 31000000 INFO @ Mon, 12 Aug 2019 22:13:11: 31000000 INFO @ Mon, 12 Aug 2019 22:13:15: 33000000 INFO @ Mon, 12 Aug 2019 22:13:18: 32000000 INFO @ Mon, 12 Aug 2019 22:13:21: 32000000 INFO @ Mon, 12 Aug 2019 22:13:23: 34000000 INFO @ Mon, 12 Aug 2019 22:13:27: 33000000 INFO @ Mon, 12 Aug 2019 22:13:30: 33000000 INFO @ Mon, 12 Aug 2019 22:13:32: 35000000 INFO @ Mon, 12 Aug 2019 22:13:36: 34000000 INFO @ Mon, 12 Aug 2019 22:13:39: 34000000 INFO @ Mon, 12 Aug 2019 22:13:41: 36000000 INFO @ Mon, 12 Aug 2019 22:13:46: 35000000 INFO @ Mon, 12 Aug 2019 22:13:49: 35000000 INFO @ Mon, 12 Aug 2019 22:13:49: 37000000 INFO @ Mon, 12 Aug 2019 22:13:55: 36000000 INFO @ Mon, 12 Aug 2019 22:13:58: 38000000 INFO @ Mon, 12 Aug 2019 22:13:58: 36000000 INFO @ Mon, 12 Aug 2019 22:14:04: 37000000 INFO @ Mon, 12 Aug 2019 22:14:07: 39000000 INFO @ Mon, 12 Aug 2019 22:14:07: 37000000 INFO @ Mon, 12 Aug 2019 22:14:13: 38000000 INFO @ Mon, 12 Aug 2019 22:14:15: 40000000 INFO @ Mon, 12 Aug 2019 22:14:17: 38000000 INFO @ Mon, 12 Aug 2019 22:14:23: 39000000 INFO @ Mon, 12 Aug 2019 22:14:24: 41000000 INFO @ Mon, 12 Aug 2019 22:14:26: 39000000 INFO @ Mon, 12 Aug 2019 22:14:32: 40000000 INFO @ Mon, 12 Aug 2019 22:14:32: 42000000 INFO @ Mon, 12 Aug 2019 22:14:35: 40000000 INFO @ Mon, 12 Aug 2019 22:14:41: 43000000 INFO @ Mon, 12 Aug 2019 22:14:41: 41000000 INFO @ Mon, 12 Aug 2019 22:14:42: #1 tag size is determined as 76 bps INFO @ Mon, 12 Aug 2019 22:14:42: #1 tag size = 76 INFO @ Mon, 12 Aug 2019 22:14:42: #1 total tags in treatment: 43012081 INFO @ Mon, 12 Aug 2019 22:14:42: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:14:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:14:42: #1 tags after filtering in treatment: 43012081 INFO @ Mon, 12 Aug 2019 22:14:42: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:14:42: #1 finished! INFO @ Mon, 12 Aug 2019 22:14:42: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:14:42: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:14:44: 41000000 INFO @ Mon, 12 Aug 2019 22:14:46: #2 number of paired peaks: 56 WARNING @ Mon, 12 Aug 2019 22:14:46: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:14:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX370362/SRX370362.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX370362/SRX370362.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX370362/SRX370362.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX370362/SRX370362.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 22:14:50: 42000000 INFO @ Mon, 12 Aug 2019 22:14:54: 42000000 INFO @ Mon, 12 Aug 2019 22:14:59: 43000000 INFO @ Mon, 12 Aug 2019 22:15:00: #1 tag size is determined as 76 bps INFO @ Mon, 12 Aug 2019 22:15:00: #1 tag size = 76 INFO @ Mon, 12 Aug 2019 22:15:00: #1 total tags in treatment: 43012081 INFO @ Mon, 12 Aug 2019 22:15:00: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:15:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:15:01: #1 tags after filtering in treatment: 43012081 INFO @ Mon, 12 Aug 2019 22:15:01: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:15:01: #1 finished! INFO @ Mon, 12 Aug 2019 22:15:01: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:15:01: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:15:03: 43000000 INFO @ Mon, 12 Aug 2019 22:15:03: #1 tag size is determined as 76 bps INFO @ Mon, 12 Aug 2019 22:15:03: #1 tag size = 76 INFO @ Mon, 12 Aug 2019 22:15:03: #1 total tags in treatment: 43012081 INFO @ Mon, 12 Aug 2019 22:15:03: #1 user defined the maximum tags... INFO @ Mon, 12 Aug 2019 22:15:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 12 Aug 2019 22:15:04: #1 tags after filtering in treatment: 43012081 INFO @ Mon, 12 Aug 2019 22:15:04: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 12 Aug 2019 22:15:04: #1 finished! INFO @ Mon, 12 Aug 2019 22:15:04: #2 Build Peak Model... INFO @ Mon, 12 Aug 2019 22:15:04: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 12 Aug 2019 22:15:04: #2 number of paired peaks: 56 WARNING @ Mon, 12 Aug 2019 22:15:04: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:15:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX370362/SRX370362.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX370362/SRX370362.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX370362/SRX370362.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX370362/SRX370362.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 12 Aug 2019 22:15:08: #2 number of paired peaks: 56 WARNING @ Mon, 12 Aug 2019 22:15:08: Too few paired peaks (56) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 12 Aug 2019 22:15:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX370362/SRX370362.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX370362/SRX370362.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX370362/SRX370362.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX370362/SRX370362.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。