Job ID = 2590325 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-08-12T15:12:31 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 105,881,026 reads read : 105,881,026 reads written : 105,881,026 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:36:33 105881026 reads; of these: 105881026 (100.00%) were unpaired; of these: 15607039 (14.74%) aligned 0 times 82558509 (77.97%) aligned exactly 1 time 7715478 (7.29%) aligned >1 times 85.26% overall alignment rate Time searching: 00:36:33 Overall time: 00:36:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 40 files... [bam_rmdupse_core] 54853330 / 90273987 = 0.6076 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 13 Aug 2019 02:37:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX370361/SRX370361.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX370361/SRX370361.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX370361/SRX370361.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX370361/SRX370361.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 13 Aug 2019 02:37:39: #1 read tag files... INFO @ Tue, 13 Aug 2019 02:37:39: #1 read treatment tags... INFO @ Tue, 13 Aug 2019 02:37:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX370361/SRX370361.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX370361/SRX370361.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX370361/SRX370361.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX370361/SRX370361.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 13 Aug 2019 02:37:40: #1 read tag files... INFO @ Tue, 13 Aug 2019 02:37:40: #1 read treatment tags... INFO @ Tue, 13 Aug 2019 02:37:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX370361/SRX370361.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX370361/SRX370361.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX370361/SRX370361.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX370361/SRX370361.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 13 Aug 2019 02:37:41: #1 read tag files... INFO @ Tue, 13 Aug 2019 02:37:41: #1 read treatment tags... INFO @ Tue, 13 Aug 2019 02:37:49: 1000000 INFO @ Tue, 13 Aug 2019 02:37:50: 1000000 INFO @ Tue, 13 Aug 2019 02:37:50: 1000000 INFO @ Tue, 13 Aug 2019 02:37:58: 2000000 INFO @ Tue, 13 Aug 2019 02:37:59: 2000000 INFO @ Tue, 13 Aug 2019 02:37:59: 2000000 INFO @ Tue, 13 Aug 2019 02:38:07: 3000000 INFO @ Tue, 13 Aug 2019 02:38:08: 3000000 INFO @ Tue, 13 Aug 2019 02:38:09: 3000000 INFO @ Tue, 13 Aug 2019 02:38:17: 4000000 INFO @ Tue, 13 Aug 2019 02:38:17: 4000000 INFO @ Tue, 13 Aug 2019 02:38:18: 4000000 INFO @ Tue, 13 Aug 2019 02:38:26: 5000000 INFO @ Tue, 13 Aug 2019 02:38:26: 5000000 INFO @ Tue, 13 Aug 2019 02:38:28: 5000000 INFO @ Tue, 13 Aug 2019 02:38:35: 6000000 INFO @ Tue, 13 Aug 2019 02:38:36: 6000000 INFO @ Tue, 13 Aug 2019 02:38:37: 6000000 INFO @ Tue, 13 Aug 2019 02:38:44: 7000000 INFO @ Tue, 13 Aug 2019 02:38:45: 7000000 INFO @ Tue, 13 Aug 2019 02:38:46: 7000000 INFO @ Tue, 13 Aug 2019 02:38:53: 8000000 INFO @ Tue, 13 Aug 2019 02:38:55: 8000000 INFO @ Tue, 13 Aug 2019 02:38:56: 8000000 INFO @ Tue, 13 Aug 2019 02:39:02: 9000000 INFO @ Tue, 13 Aug 2019 02:39:04: 9000000 INFO @ Tue, 13 Aug 2019 02:39:05: 9000000 INFO @ Tue, 13 Aug 2019 02:39:11: 10000000 INFO @ Tue, 13 Aug 2019 02:39:14: 10000000 INFO @ Tue, 13 Aug 2019 02:39:15: 10000000 INFO @ Tue, 13 Aug 2019 02:39:20: 11000000 INFO @ Tue, 13 Aug 2019 02:39:23: 11000000 INFO @ Tue, 13 Aug 2019 02:39:24: 11000000 INFO @ Tue, 13 Aug 2019 02:39:30: 12000000 INFO @ Tue, 13 Aug 2019 02:39:33: 12000000 INFO @ Tue, 13 Aug 2019 02:39:34: 12000000 INFO @ Tue, 13 Aug 2019 02:39:40: 13000000 INFO @ Tue, 13 Aug 2019 02:39:43: 13000000 INFO @ Tue, 13 Aug 2019 02:39:43: 13000000 INFO @ Tue, 13 Aug 2019 02:39:49: 14000000 INFO @ Tue, 13 Aug 2019 02:39:52: 14000000 INFO @ Tue, 13 Aug 2019 02:39:53: 14000000 INFO @ Tue, 13 Aug 2019 02:39:59: 15000000 INFO @ Tue, 13 Aug 2019 02:40:01: 15000000 INFO @ Tue, 13 Aug 2019 02:40:02: 15000000 INFO @ Tue, 13 Aug 2019 02:40:08: 16000000 INFO @ Tue, 13 Aug 2019 02:40:09: 16000000 INFO @ Tue, 13 Aug 2019 02:40:12: 16000000 INFO @ Tue, 13 Aug 2019 02:40:18: 17000000 INFO @ Tue, 13 Aug 2019 02:40:18: 17000000 INFO @ Tue, 13 Aug 2019 02:40:21: 17000000 INFO @ Tue, 13 Aug 2019 02:40:27: 18000000 INFO @ Tue, 13 Aug 2019 02:40:27: 18000000 INFO @ Tue, 13 Aug 2019 02:40:30: 18000000 INFO @ Tue, 13 Aug 2019 02:40:36: 19000000 INFO @ Tue, 13 Aug 2019 02:40:37: 19000000 INFO @ Tue, 13 Aug 2019 02:40:40: 19000000 INFO @ Tue, 13 Aug 2019 02:40:45: 20000000 INFO @ Tue, 13 Aug 2019 02:40:46: 20000000 INFO @ Tue, 13 Aug 2019 02:40:49: 20000000 INFO @ Tue, 13 Aug 2019 02:40:54: 21000000 INFO @ Tue, 13 Aug 2019 02:40:56: 21000000 INFO @ Tue, 13 Aug 2019 02:40:59: 21000000 INFO @ Tue, 13 Aug 2019 02:41:03: 22000000 INFO @ Tue, 13 Aug 2019 02:41:05: 22000000 INFO @ Tue, 13 Aug 2019 02:41:08: 22000000 INFO @ Tue, 13 Aug 2019 02:41:12: 23000000 INFO @ Tue, 13 Aug 2019 02:41:15: 23000000 INFO @ Tue, 13 Aug 2019 02:41:18: 23000000 INFO @ Tue, 13 Aug 2019 02:41:22: 24000000 INFO @ Tue, 13 Aug 2019 02:41:25: 24000000 INFO @ Tue, 13 Aug 2019 02:41:28: 24000000 INFO @ Tue, 13 Aug 2019 02:41:32: 25000000 INFO @ Tue, 13 Aug 2019 02:41:35: 25000000 INFO @ Tue, 13 Aug 2019 02:41:38: 25000000 INFO @ Tue, 13 Aug 2019 02:41:42: 26000000 INFO @ Tue, 13 Aug 2019 02:41:45: 26000000 INFO @ Tue, 13 Aug 2019 02:41:48: 26000000 INFO @ Tue, 13 Aug 2019 02:41:52: 27000000 INFO @ Tue, 13 Aug 2019 02:41:56: 27000000 INFO @ Tue, 13 Aug 2019 02:41:58: 27000000 INFO @ Tue, 13 Aug 2019 02:42:02: 28000000 INFO @ Tue, 13 Aug 2019 02:42:06: 28000000 INFO @ Tue, 13 Aug 2019 02:42:09: 28000000 INFO @ Tue, 13 Aug 2019 02:42:12: 29000000 INFO @ Tue, 13 Aug 2019 02:42:16: 29000000 INFO @ Tue, 13 Aug 2019 02:42:19: 29000000 INFO @ Tue, 13 Aug 2019 02:42:22: 30000000 INFO @ Tue, 13 Aug 2019 02:42:26: 30000000 INFO @ Tue, 13 Aug 2019 02:42:29: 30000000 INFO @ Tue, 13 Aug 2019 02:42:32: 31000000 INFO @ Tue, 13 Aug 2019 02:42:36: 31000000 INFO @ Tue, 13 Aug 2019 02:42:39: 31000000 INFO @ Tue, 13 Aug 2019 02:42:42: 32000000 INFO @ Tue, 13 Aug 2019 02:42:45: 32000000 INFO @ Tue, 13 Aug 2019 02:42:49: 32000000 INFO @ Tue, 13 Aug 2019 02:42:52: 33000000 INFO @ Tue, 13 Aug 2019 02:42:55: 33000000 INFO @ Tue, 13 Aug 2019 02:42:59: 33000000 INFO @ Tue, 13 Aug 2019 02:43:02: 34000000 INFO @ Tue, 13 Aug 2019 02:43:05: 34000000 INFO @ Tue, 13 Aug 2019 02:43:09: 34000000 INFO @ Tue, 13 Aug 2019 02:43:11: 35000000 INFO @ Tue, 13 Aug 2019 02:43:15: 35000000 INFO @ Tue, 13 Aug 2019 02:43:16: #1 tag size is determined as 76 bps INFO @ Tue, 13 Aug 2019 02:43:16: #1 tag size = 76 INFO @ Tue, 13 Aug 2019 02:43:16: #1 total tags in treatment: 35420657 INFO @ Tue, 13 Aug 2019 02:43:16: #1 user defined the maximum tags... INFO @ Tue, 13 Aug 2019 02:43:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 13 Aug 2019 02:43:16: #1 tags after filtering in treatment: 35420657 INFO @ Tue, 13 Aug 2019 02:43:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 13 Aug 2019 02:43:16: #1 finished! INFO @ Tue, 13 Aug 2019 02:43:16: #2 Build Peak Model... INFO @ Tue, 13 Aug 2019 02:43:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 13 Aug 2019 02:43:19: 35000000 INFO @ Tue, 13 Aug 2019 02:43:19: #1 tag size is determined as 76 bps INFO @ Tue, 13 Aug 2019 02:43:19: #1 tag size = 76 INFO @ Tue, 13 Aug 2019 02:43:19: #1 total tags in treatment: 35420657 INFO @ Tue, 13 Aug 2019 02:43:19: #1 user defined the maximum tags... INFO @ Tue, 13 Aug 2019 02:43:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 13 Aug 2019 02:43:19: #2 number of paired peaks: 80 WARNING @ Tue, 13 Aug 2019 02:43:19: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 13 Aug 2019 02:43:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX370361/SRX370361.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX370361/SRX370361.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX370361/SRX370361.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX370361/SRX370361.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 13 Aug 2019 02:43:20: #1 tags after filtering in treatment: 35420657 INFO @ Tue, 13 Aug 2019 02:43:20: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 13 Aug 2019 02:43:20: #1 finished! INFO @ Tue, 13 Aug 2019 02:43:20: #2 Build Peak Model... INFO @ Tue, 13 Aug 2019 02:43:20: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 13 Aug 2019 02:43:23: #1 tag size is determined as 76 bps INFO @ Tue, 13 Aug 2019 02:43:23: #1 tag size = 76 INFO @ Tue, 13 Aug 2019 02:43:23: #1 total tags in treatment: 35420657 INFO @ Tue, 13 Aug 2019 02:43:23: #1 user defined the maximum tags... INFO @ Tue, 13 Aug 2019 02:43:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 13 Aug 2019 02:43:23: #2 number of paired peaks: 80 WARNING @ Tue, 13 Aug 2019 02:43:23: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 13 Aug 2019 02:43:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX370361/SRX370361.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX370361/SRX370361.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX370361/SRX370361.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX370361/SRX370361.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 13 Aug 2019 02:43:23: #1 tags after filtering in treatment: 35420657 INFO @ Tue, 13 Aug 2019 02:43:23: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 13 Aug 2019 02:43:23: #1 finished! INFO @ Tue, 13 Aug 2019 02:43:23: #2 Build Peak Model... INFO @ Tue, 13 Aug 2019 02:43:23: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 13 Aug 2019 02:43:26: #2 number of paired peaks: 80 WARNING @ Tue, 13 Aug 2019 02:43:26: Too few paired peaks (80) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 13 Aug 2019 02:43:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX370361/SRX370361.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX370361/SRX370361.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX370361/SRX370361.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX370361/SRX370361.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。