Job ID = 6527998 SRX = SRX3663162 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:09:51 prefetch.2.10.7: 1) Downloading 'SRR6686972'... 2020-06-29T14:09:51 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:14:38 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:14:38 prefetch.2.10.7: 1) 'SRR6686972' was downloaded successfully Read 39907980 spots for SRR6686972/SRR6686972.sra Written 39907980 spots for SRR6686972/SRR6686972.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:10:21 39907980 reads; of these: 39907980 (100.00%) were unpaired; of these: 5426784 (13.60%) aligned 0 times 28880746 (72.37%) aligned exactly 1 time 5600450 (14.03%) aligned >1 times 86.40% overall alignment rate Time searching: 00:10:22 Overall time: 00:10:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4637454 / 34481196 = 0.1345 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:50:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3663162/SRX3663162.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3663162/SRX3663162.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3663162/SRX3663162.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3663162/SRX3663162.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:50:32: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:50:32: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:50:38: 1000000 INFO @ Mon, 29 Jun 2020 23:50:44: 2000000 INFO @ Mon, 29 Jun 2020 23:50:49: 3000000 INFO @ Mon, 29 Jun 2020 23:50:55: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:51:01: 5000000 INFO @ Mon, 29 Jun 2020 23:51:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3663162/SRX3663162.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3663162/SRX3663162.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3663162/SRX3663162.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3663162/SRX3663162.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:51:02: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:51:02: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:51:07: 6000000 INFO @ Mon, 29 Jun 2020 23:51:08: 1000000 INFO @ Mon, 29 Jun 2020 23:51:13: 7000000 INFO @ Mon, 29 Jun 2020 23:51:15: 2000000 INFO @ Mon, 29 Jun 2020 23:51:20: 8000000 INFO @ Mon, 29 Jun 2020 23:51:21: 3000000 INFO @ Mon, 29 Jun 2020 23:51:26: 9000000 INFO @ Mon, 29 Jun 2020 23:51:27: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:51:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3663162/SRX3663162.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3663162/SRX3663162.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3663162/SRX3663162.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3663162/SRX3663162.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:51:32: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:51:32: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:51:32: 10000000 INFO @ Mon, 29 Jun 2020 23:51:34: 5000000 INFO @ Mon, 29 Jun 2020 23:51:39: 11000000 INFO @ Mon, 29 Jun 2020 23:51:39: 1000000 INFO @ Mon, 29 Jun 2020 23:51:40: 6000000 INFO @ Mon, 29 Jun 2020 23:51:45: 12000000 INFO @ Mon, 29 Jun 2020 23:51:45: 2000000 INFO @ Mon, 29 Jun 2020 23:51:47: 7000000 INFO @ Mon, 29 Jun 2020 23:51:51: 13000000 INFO @ Mon, 29 Jun 2020 23:51:52: 3000000 INFO @ Mon, 29 Jun 2020 23:51:53: 8000000 INFO @ Mon, 29 Jun 2020 23:51:58: 14000000 INFO @ Mon, 29 Jun 2020 23:51:58: 4000000 INFO @ Mon, 29 Jun 2020 23:52:00: 9000000 INFO @ Mon, 29 Jun 2020 23:52:04: 15000000 INFO @ Mon, 29 Jun 2020 23:52:05: 5000000 INFO @ Mon, 29 Jun 2020 23:52:06: 10000000 INFO @ Mon, 29 Jun 2020 23:52:11: 16000000 INFO @ Mon, 29 Jun 2020 23:52:11: 6000000 INFO @ Mon, 29 Jun 2020 23:52:13: 11000000 INFO @ Mon, 29 Jun 2020 23:52:17: 17000000 INFO @ Mon, 29 Jun 2020 23:52:17: 7000000 INFO @ Mon, 29 Jun 2020 23:52:19: 12000000 INFO @ Mon, 29 Jun 2020 23:52:23: 18000000 INFO @ Mon, 29 Jun 2020 23:52:24: 8000000 INFO @ Mon, 29 Jun 2020 23:52:26: 13000000 INFO @ Mon, 29 Jun 2020 23:52:30: 19000000 INFO @ Mon, 29 Jun 2020 23:52:30: 9000000 INFO @ Mon, 29 Jun 2020 23:52:32: 14000000 INFO @ Mon, 29 Jun 2020 23:52:36: 20000000 INFO @ Mon, 29 Jun 2020 23:52:37: 10000000 INFO @ Mon, 29 Jun 2020 23:52:38: 15000000 INFO @ Mon, 29 Jun 2020 23:52:42: 21000000 INFO @ Mon, 29 Jun 2020 23:52:44: 11000000 INFO @ Mon, 29 Jun 2020 23:52:45: 16000000 INFO @ Mon, 29 Jun 2020 23:52:49: 22000000 INFO @ Mon, 29 Jun 2020 23:52:50: 12000000 INFO @ Mon, 29 Jun 2020 23:52:51: 17000000 INFO @ Mon, 29 Jun 2020 23:52:55: 23000000 INFO @ Mon, 29 Jun 2020 23:52:57: 13000000 INFO @ Mon, 29 Jun 2020 23:52:57: 18000000 INFO @ Mon, 29 Jun 2020 23:53:01: 24000000 INFO @ Mon, 29 Jun 2020 23:53:04: 19000000 INFO @ Mon, 29 Jun 2020 23:53:04: 14000000 INFO @ Mon, 29 Jun 2020 23:53:07: 25000000 INFO @ Mon, 29 Jun 2020 23:53:10: 20000000 INFO @ Mon, 29 Jun 2020 23:53:11: 15000000 INFO @ Mon, 29 Jun 2020 23:53:13: 26000000 INFO @ Mon, 29 Jun 2020 23:53:16: 21000000 INFO @ Mon, 29 Jun 2020 23:53:18: 16000000 INFO @ Mon, 29 Jun 2020 23:53:19: 27000000 INFO @ Mon, 29 Jun 2020 23:53:22: 22000000 INFO @ Mon, 29 Jun 2020 23:53:25: 28000000 INFO @ Mon, 29 Jun 2020 23:53:26: 17000000 INFO @ Mon, 29 Jun 2020 23:53:29: 23000000 INFO @ Mon, 29 Jun 2020 23:53:31: 29000000 INFO @ Mon, 29 Jun 2020 23:53:33: 18000000 INFO @ Mon, 29 Jun 2020 23:53:35: 24000000 INFO @ Mon, 29 Jun 2020 23:53:37: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:53:37: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:53:37: #1 total tags in treatment: 29843742 INFO @ Mon, 29 Jun 2020 23:53:37: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:53:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:53:37: #1 tags after filtering in treatment: 29843742 INFO @ Mon, 29 Jun 2020 23:53:37: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:53:37: #1 finished! INFO @ Mon, 29 Jun 2020 23:53:37: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:53:37: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:53:39: #2 number of paired peaks: 82 WARNING @ Mon, 29 Jun 2020 23:53:39: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:53:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3663162/SRX3663162.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663162/SRX3663162.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663162/SRX3663162.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663162/SRX3663162.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:53:40: 19000000 INFO @ Mon, 29 Jun 2020 23:53:41: 25000000 INFO @ Mon, 29 Jun 2020 23:53:46: 20000000 INFO @ Mon, 29 Jun 2020 23:53:47: 26000000 INFO @ Mon, 29 Jun 2020 23:53:52: 21000000 INFO @ Mon, 29 Jun 2020 23:53:53: 27000000 INFO @ Mon, 29 Jun 2020 23:53:58: 22000000 INFO @ Mon, 29 Jun 2020 23:53:59: 28000000 INFO @ Mon, 29 Jun 2020 23:54:04: 23000000 INFO @ Mon, 29 Jun 2020 23:54:05: 29000000 INFO @ Mon, 29 Jun 2020 23:54:10: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:54:10: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:54:10: #1 total tags in treatment: 29843742 INFO @ Mon, 29 Jun 2020 23:54:10: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:54:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:54:10: 24000000 INFO @ Mon, 29 Jun 2020 23:54:11: #1 tags after filtering in treatment: 29843742 INFO @ Mon, 29 Jun 2020 23:54:11: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:54:11: #1 finished! INFO @ Mon, 29 Jun 2020 23:54:11: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:54:11: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:54:13: #2 number of paired peaks: 82 WARNING @ Mon, 29 Jun 2020 23:54:13: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:54:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3663162/SRX3663162.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663162/SRX3663162.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663162/SRX3663162.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663162/SRX3663162.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:54:16: 25000000 INFO @ Mon, 29 Jun 2020 23:54:21: 26000000 INFO @ Mon, 29 Jun 2020 23:54:27: 27000000 INFO @ Mon, 29 Jun 2020 23:54:32: 28000000 INFO @ Mon, 29 Jun 2020 23:54:37: 29000000 INFO @ Mon, 29 Jun 2020 23:54:42: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:54:42: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:54:42: #1 total tags in treatment: 29843742 INFO @ Mon, 29 Jun 2020 23:54:42: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:54:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:54:43: #1 tags after filtering in treatment: 29843742 INFO @ Mon, 29 Jun 2020 23:54:43: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:54:43: #1 finished! INFO @ Mon, 29 Jun 2020 23:54:43: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:54:43: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:54:44: #2 number of paired peaks: 82 WARNING @ Mon, 29 Jun 2020 23:54:44: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:54:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3663162/SRX3663162.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663162/SRX3663162.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663162/SRX3663162.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663162/SRX3663162.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。