Job ID = 6527996 SRX = SRX3663161 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T14:06:48 prefetch.2.10.7: 1) Downloading 'SRR6686971'... 2020-06-29T14:06:48 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T14:12:51 prefetch.2.10.7: HTTPS download succeed 2020-06-29T14:12:51 prefetch.2.10.7: 1) 'SRR6686971' was downloaded successfully Read 37745602 spots for SRR6686971/SRR6686971.sra Written 37745602 spots for SRR6686971/SRR6686971.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:57 37745602 reads; of these: 37745602 (100.00%) were unpaired; of these: 4297084 (11.38%) aligned 0 times 28152820 (74.59%) aligned exactly 1 time 5295698 (14.03%) aligned >1 times 88.62% overall alignment rate Time searching: 00:09:57 Overall time: 00:09:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 5291896 / 33448518 = 0.1582 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:47:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3663161/SRX3663161.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3663161/SRX3663161.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3663161/SRX3663161.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3663161/SRX3663161.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:47:42: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:47:42: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:47:50: 1000000 INFO @ Mon, 29 Jun 2020 23:47:56: 2000000 INFO @ Mon, 29 Jun 2020 23:48:04: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:48:12: 4000000 INFO @ Mon, 29 Jun 2020 23:48:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3663161/SRX3663161.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3663161/SRX3663161.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3663161/SRX3663161.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3663161/SRX3663161.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:48:12: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:48:12: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:48:19: 5000000 INFO @ Mon, 29 Jun 2020 23:48:20: 1000000 INFO @ Mon, 29 Jun 2020 23:48:25: 6000000 INFO @ Mon, 29 Jun 2020 23:48:29: 2000000 INFO @ Mon, 29 Jun 2020 23:48:32: 7000000 INFO @ Mon, 29 Jun 2020 23:48:38: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:48:41: 8000000 INFO @ Mon, 29 Jun 2020 23:48:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3663161/SRX3663161.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3663161/SRX3663161.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3663161/SRX3663161.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3663161/SRX3663161.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:48:42: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:48:42: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:48:46: 4000000 INFO @ Mon, 29 Jun 2020 23:48:49: 1000000 INFO @ Mon, 29 Jun 2020 23:48:50: 9000000 INFO @ Mon, 29 Jun 2020 23:48:55: 5000000 INFO @ Mon, 29 Jun 2020 23:48:56: 2000000 INFO @ Mon, 29 Jun 2020 23:48:58: 10000000 INFO @ Mon, 29 Jun 2020 23:49:02: 3000000 INFO @ Mon, 29 Jun 2020 23:49:03: 6000000 INFO @ Mon, 29 Jun 2020 23:49:07: 11000000 INFO @ Mon, 29 Jun 2020 23:49:10: 4000000 INFO @ Mon, 29 Jun 2020 23:49:12: 7000000 INFO @ Mon, 29 Jun 2020 23:49:15: 12000000 INFO @ Mon, 29 Jun 2020 23:49:17: 5000000 INFO @ Mon, 29 Jun 2020 23:49:20: 8000000 INFO @ Mon, 29 Jun 2020 23:49:24: 6000000 INFO @ Mon, 29 Jun 2020 23:49:24: 13000000 INFO @ Mon, 29 Jun 2020 23:49:29: 9000000 INFO @ Mon, 29 Jun 2020 23:49:31: 7000000 INFO @ Mon, 29 Jun 2020 23:49:32: 14000000 INFO @ Mon, 29 Jun 2020 23:49:38: 8000000 INFO @ Mon, 29 Jun 2020 23:49:38: 10000000 INFO @ Mon, 29 Jun 2020 23:49:41: 15000000 INFO @ Mon, 29 Jun 2020 23:49:45: 9000000 INFO @ Mon, 29 Jun 2020 23:49:46: 11000000 INFO @ Mon, 29 Jun 2020 23:49:50: 16000000 INFO @ Mon, 29 Jun 2020 23:49:52: 10000000 INFO @ Mon, 29 Jun 2020 23:49:55: 12000000 INFO @ Mon, 29 Jun 2020 23:49:59: 11000000 INFO @ Mon, 29 Jun 2020 23:49:59: 17000000 INFO @ Mon, 29 Jun 2020 23:50:04: 13000000 INFO @ Mon, 29 Jun 2020 23:50:06: 12000000 INFO @ Mon, 29 Jun 2020 23:50:08: 18000000 INFO @ Mon, 29 Jun 2020 23:50:12: 14000000 INFO @ Mon, 29 Jun 2020 23:50:13: 13000000 INFO @ Mon, 29 Jun 2020 23:50:17: 19000000 INFO @ Mon, 29 Jun 2020 23:50:20: 14000000 INFO @ Mon, 29 Jun 2020 23:50:20: 15000000 INFO @ Mon, 29 Jun 2020 23:50:25: 20000000 INFO @ Mon, 29 Jun 2020 23:50:27: 15000000 INFO @ Mon, 29 Jun 2020 23:50:29: 16000000 INFO @ Mon, 29 Jun 2020 23:50:33: 21000000 INFO @ Mon, 29 Jun 2020 23:50:35: 16000000 INFO @ Mon, 29 Jun 2020 23:50:37: 17000000 INFO @ Mon, 29 Jun 2020 23:50:40: 22000000 INFO @ Mon, 29 Jun 2020 23:50:42: 17000000 INFO @ Mon, 29 Jun 2020 23:50:45: 18000000 INFO @ Mon, 29 Jun 2020 23:50:48: 23000000 INFO @ Mon, 29 Jun 2020 23:50:49: 18000000 INFO @ Mon, 29 Jun 2020 23:50:53: 19000000 INFO @ Mon, 29 Jun 2020 23:50:55: 24000000 INFO @ Mon, 29 Jun 2020 23:50:57: 19000000 INFO @ Mon, 29 Jun 2020 23:51:01: 20000000 INFO @ Mon, 29 Jun 2020 23:51:03: 25000000 INFO @ Mon, 29 Jun 2020 23:51:04: 20000000 INFO @ Mon, 29 Jun 2020 23:51:09: 21000000 INFO @ Mon, 29 Jun 2020 23:51:10: 26000000 INFO @ Mon, 29 Jun 2020 23:51:11: 21000000 INFO @ Mon, 29 Jun 2020 23:51:17: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:51:18: 27000000 INFO @ Mon, 29 Jun 2020 23:51:19: 22000000 INFO @ Mon, 29 Jun 2020 23:51:24: 23000000 INFO @ Mon, 29 Jun 2020 23:51:25: 28000000 INFO @ Mon, 29 Jun 2020 23:51:26: 23000000 INFO @ Mon, 29 Jun 2020 23:51:26: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:51:26: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:51:26: #1 total tags in treatment: 28156622 INFO @ Mon, 29 Jun 2020 23:51:26: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:51:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:51:27: #1 tags after filtering in treatment: 28156622 INFO @ Mon, 29 Jun 2020 23:51:27: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:51:27: #1 finished! INFO @ Mon, 29 Jun 2020 23:51:27: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:51:27: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:51:29: #2 number of paired peaks: 86 WARNING @ Mon, 29 Jun 2020 23:51:29: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:51:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3663161/SRX3663161.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663161/SRX3663161.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663161/SRX3663161.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663161/SRX3663161.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:51:31: 24000000 INFO @ Mon, 29 Jun 2020 23:51:32: 24000000 INFO @ Mon, 29 Jun 2020 23:51:38: 25000000 INFO @ Mon, 29 Jun 2020 23:51:39: 25000000 INFO @ Mon, 29 Jun 2020 23:51:44: 26000000 INFO @ Mon, 29 Jun 2020 23:51:45: 26000000 INFO @ Mon, 29 Jun 2020 23:51:51: 27000000 INFO @ Mon, 29 Jun 2020 23:51:52: 27000000 INFO @ Mon, 29 Jun 2020 23:51:58: 28000000 INFO @ Mon, 29 Jun 2020 23:51:58: 28000000 INFO @ Mon, 29 Jun 2020 23:51:59: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:51:59: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:51:59: #1 total tags in treatment: 28156622 INFO @ Mon, 29 Jun 2020 23:51:59: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:51:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:51:59: #1 tags after filtering in treatment: 28156622 INFO @ Mon, 29 Jun 2020 23:51:59: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:51:59: #1 finished! INFO @ Mon, 29 Jun 2020 23:51:59: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:51:59: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:52:00: #1 tag size is determined as 51 bps INFO @ Mon, 29 Jun 2020 23:52:00: #1 tag size = 51 INFO @ Mon, 29 Jun 2020 23:52:00: #1 total tags in treatment: 28156622 INFO @ Mon, 29 Jun 2020 23:52:00: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:52:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:52:00: #1 tags after filtering in treatment: 28156622 INFO @ Mon, 29 Jun 2020 23:52:00: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:52:00: #1 finished! INFO @ Mon, 29 Jun 2020 23:52:00: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:52:00: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:52:01: #2 number of paired peaks: 86 WARNING @ Mon, 29 Jun 2020 23:52:01: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:52:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3663161/SRX3663161.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663161/SRX3663161.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663161/SRX3663161.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663161/SRX3663161.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:52:02: #2 number of paired peaks: 86 WARNING @ Mon, 29 Jun 2020 23:52:02: Too few paired peaks (86) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:52:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX3663161/SRX3663161.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663161/SRX3663161.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663161/SRX3663161.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX3663161/SRX3663161.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。