Job ID = 11240722 sra ファイルのダウンロード中... Completed: 1628329K bytes transferred in 71 seconds (185953K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 79827204 spots for /home/okishinya/chipatlas/results/dm3/SRX3630331/SRR6652853.sra Written 79827204 spots for /home/okishinya/chipatlas/results/dm3/SRX3630331/SRR6652853.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:30:28 79827204 reads; of these: 79827204 (100.00%) were unpaired; of these: 3762477 (4.71%) aligned 0 times 57559563 (72.11%) aligned exactly 1 time 18505164 (23.18%) aligned >1 times 95.29% overall alignment rate Time searching: 00:30:28 Overall time: 00:30:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 16738596 / 76064727 = 0.2201 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 07 Oct 2018 21:24:48: # Command line: callpeak -t SRX3630331.bam -f BAM -g dm -n SRX3630331.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3630331.20 # format = BAM # ChIP-seq file = ['SRX3630331.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 07 Oct 2018 21:24:48: # Command line: callpeak -t SRX3630331.bam -f BAM -g dm -n SRX3630331.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3630331.05 # format = BAM # ChIP-seq file = ['SRX3630331.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 07 Oct 2018 21:24:48: #1 read tag files... INFO @ Sun, 07 Oct 2018 21:24:48: #1 read tag files... INFO @ Sun, 07 Oct 2018 21:24:48: #1 read treatment tags... INFO @ Sun, 07 Oct 2018 21:24:48: #1 read treatment tags... INFO @ Sun, 07 Oct 2018 21:24:48: # Command line: callpeak -t SRX3630331.bam -f BAM -g dm -n SRX3630331.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3630331.10 # format = BAM # ChIP-seq file = ['SRX3630331.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 07 Oct 2018 21:24:48: #1 read tag files... INFO @ Sun, 07 Oct 2018 21:24:48: #1 read treatment tags... INFO @ Sun, 07 Oct 2018 21:24:55: 1000000 INFO @ Sun, 07 Oct 2018 21:24:55: 1000000 INFO @ Sun, 07 Oct 2018 21:24:55: 1000000 INFO @ Sun, 07 Oct 2018 21:25:02: 2000000 INFO @ Sun, 07 Oct 2018 21:25:02: 2000000 INFO @ Sun, 07 Oct 2018 21:25:02: 2000000 INFO @ Sun, 07 Oct 2018 21:25:09: 3000000 INFO @ Sun, 07 Oct 2018 21:25:09: 3000000 INFO @ Sun, 07 Oct 2018 21:25:09: 3000000 INFO @ Sun, 07 Oct 2018 21:25:16: 4000000 INFO @ Sun, 07 Oct 2018 21:25:16: 4000000 INFO @ Sun, 07 Oct 2018 21:25:16: 4000000 INFO @ Sun, 07 Oct 2018 21:25:23: 5000000 INFO @ Sun, 07 Oct 2018 21:25:23: 5000000 INFO @ Sun, 07 Oct 2018 21:25:23: 5000000 INFO @ Sun, 07 Oct 2018 21:25:30: 6000000 INFO @ Sun, 07 Oct 2018 21:25:30: 6000000 INFO @ Sun, 07 Oct 2018 21:25:30: 6000000 INFO @ Sun, 07 Oct 2018 21:25:37: 7000000 INFO @ Sun, 07 Oct 2018 21:25:37: 7000000 INFO @ Sun, 07 Oct 2018 21:25:37: 7000000 INFO @ Sun, 07 Oct 2018 21:25:44: 8000000 INFO @ Sun, 07 Oct 2018 21:25:44: 8000000 INFO @ Sun, 07 Oct 2018 21:25:45: 8000000 INFO @ Sun, 07 Oct 2018 21:25:51: 9000000 INFO @ Sun, 07 Oct 2018 21:25:51: 9000000 INFO @ Sun, 07 Oct 2018 21:25:52: 9000000 INFO @ Sun, 07 Oct 2018 21:25:57: 10000000 INFO @ Sun, 07 Oct 2018 21:25:58: 10000000 INFO @ Sun, 07 Oct 2018 21:25:59: 10000000 INFO @ Sun, 07 Oct 2018 21:26:04: 11000000 INFO @ Sun, 07 Oct 2018 21:26:05: 11000000 INFO @ Sun, 07 Oct 2018 21:26:06: 11000000 INFO @ Sun, 07 Oct 2018 21:26:12: 12000000 INFO @ Sun, 07 Oct 2018 21:26:12: 12000000 INFO @ Sun, 07 Oct 2018 21:26:13: 12000000 INFO @ Sun, 07 Oct 2018 21:26:19: 13000000 INFO @ Sun, 07 Oct 2018 21:26:19: 13000000 INFO @ Sun, 07 Oct 2018 21:26:20: 13000000 INFO @ Sun, 07 Oct 2018 21:26:26: 14000000 INFO @ Sun, 07 Oct 2018 21:26:27: 14000000 INFO @ Sun, 07 Oct 2018 21:26:27: 14000000 INFO @ Sun, 07 Oct 2018 21:26:33: 15000000 INFO @ Sun, 07 Oct 2018 21:26:34: 15000000 INFO @ Sun, 07 Oct 2018 21:26:34: 15000000 INFO @ Sun, 07 Oct 2018 21:26:40: 16000000 INFO @ Sun, 07 Oct 2018 21:26:41: 16000000 INFO @ Sun, 07 Oct 2018 21:26:41: 16000000 INFO @ Sun, 07 Oct 2018 21:26:47: 17000000 INFO @ Sun, 07 Oct 2018 21:26:48: 17000000 INFO @ Sun, 07 Oct 2018 21:26:48: 17000000 INFO @ Sun, 07 Oct 2018 21:26:54: 18000000 INFO @ Sun, 07 Oct 2018 21:26:55: 18000000 INFO @ Sun, 07 Oct 2018 21:26:55: 18000000 INFO @ Sun, 07 Oct 2018 21:27:02: 19000000 INFO @ Sun, 07 Oct 2018 21:27:02: 19000000 INFO @ Sun, 07 Oct 2018 21:27:02: 19000000 INFO @ Sun, 07 Oct 2018 21:27:09: 20000000 INFO @ Sun, 07 Oct 2018 21:27:09: 20000000 INFO @ Sun, 07 Oct 2018 21:27:10: 20000000 INFO @ Sun, 07 Oct 2018 21:27:16: 21000000 INFO @ Sun, 07 Oct 2018 21:27:16: 21000000 INFO @ Sun, 07 Oct 2018 21:27:17: 21000000 INFO @ Sun, 07 Oct 2018 21:27:23: 22000000 INFO @ Sun, 07 Oct 2018 21:27:24: 22000000 INFO @ Sun, 07 Oct 2018 21:27:24: 22000000 INFO @ Sun, 07 Oct 2018 21:27:30: 23000000 INFO @ Sun, 07 Oct 2018 21:27:31: 23000000 INFO @ Sun, 07 Oct 2018 21:27:32: 23000000 INFO @ Sun, 07 Oct 2018 21:27:36: 24000000 INFO @ Sun, 07 Oct 2018 21:27:38: 24000000 INFO @ Sun, 07 Oct 2018 21:27:39: 24000000 INFO @ Sun, 07 Oct 2018 21:27:43: 25000000 INFO @ Sun, 07 Oct 2018 21:27:46: 25000000 INFO @ Sun, 07 Oct 2018 21:27:47: 25000000 INFO @ Sun, 07 Oct 2018 21:27:50: 26000000 INFO @ Sun, 07 Oct 2018 21:27:53: 26000000 INFO @ Sun, 07 Oct 2018 21:27:54: 26000000 INFO @ Sun, 07 Oct 2018 21:27:56: 27000000 INFO @ Sun, 07 Oct 2018 21:28:01: 27000000 INFO @ Sun, 07 Oct 2018 21:28:02: 27000000 INFO @ Sun, 07 Oct 2018 21:28:03: 28000000 INFO @ Sun, 07 Oct 2018 21:28:08: 28000000 INFO @ Sun, 07 Oct 2018 21:28:09: 28000000 INFO @ Sun, 07 Oct 2018 21:28:10: 29000000 INFO @ Sun, 07 Oct 2018 21:28:16: 29000000 INFO @ Sun, 07 Oct 2018 21:28:16: 30000000 INFO @ Sun, 07 Oct 2018 21:28:17: 29000000 INFO @ Sun, 07 Oct 2018 21:28:23: 31000000 INFO @ Sun, 07 Oct 2018 21:28:23: 30000000 INFO @ Sun, 07 Oct 2018 21:28:24: 30000000 INFO @ Sun, 07 Oct 2018 21:28:30: 32000000 INFO @ Sun, 07 Oct 2018 21:28:31: 31000000 INFO @ Sun, 07 Oct 2018 21:28:32: 31000000 INFO @ Sun, 07 Oct 2018 21:28:36: 33000000 INFO @ Sun, 07 Oct 2018 21:28:38: 32000000 INFO @ Sun, 07 Oct 2018 21:28:39: 32000000 INFO @ Sun, 07 Oct 2018 21:28:43: 34000000 INFO @ Sun, 07 Oct 2018 21:28:46: 33000000 INFO @ Sun, 07 Oct 2018 21:28:46: 33000000 INFO @ Sun, 07 Oct 2018 21:28:50: 35000000 INFO @ Sun, 07 Oct 2018 21:28:53: 34000000 INFO @ Sun, 07 Oct 2018 21:28:54: 34000000 INFO @ Sun, 07 Oct 2018 21:28:57: 36000000 INFO @ Sun, 07 Oct 2018 21:29:00: 35000000 INFO @ Sun, 07 Oct 2018 21:29:01: 35000000 INFO @ Sun, 07 Oct 2018 21:29:04: 37000000 INFO @ Sun, 07 Oct 2018 21:29:07: 36000000 INFO @ Sun, 07 Oct 2018 21:29:08: 36000000 INFO @ Sun, 07 Oct 2018 21:29:11: 38000000 INFO @ Sun, 07 Oct 2018 21:29:15: 37000000 INFO @ Sun, 07 Oct 2018 21:29:15: 37000000 INFO @ Sun, 07 Oct 2018 21:29:18: 39000000 INFO @ Sun, 07 Oct 2018 21:29:22: 38000000 INFO @ Sun, 07 Oct 2018 21:29:22: 38000000 INFO @ Sun, 07 Oct 2018 21:29:26: 40000000 INFO @ Sun, 07 Oct 2018 21:29:29: 39000000 INFO @ Sun, 07 Oct 2018 21:29:29: 39000000 INFO @ Sun, 07 Oct 2018 21:29:32: 41000000 INFO @ Sun, 07 Oct 2018 21:29:36: 40000000 INFO @ Sun, 07 Oct 2018 21:29:36: 40000000 INFO @ Sun, 07 Oct 2018 21:29:39: 42000000 INFO @ Sun, 07 Oct 2018 21:29:44: 41000000 INFO @ Sun, 07 Oct 2018 21:29:44: 41000000 INFO @ Sun, 07 Oct 2018 21:29:46: 43000000 INFO @ Sun, 07 Oct 2018 21:29:51: 42000000 INFO @ Sun, 07 Oct 2018 21:29:51: 42000000 INFO @ Sun, 07 Oct 2018 21:29:52: 44000000 INFO @ Sun, 07 Oct 2018 21:29:58: 43000000 INFO @ Sun, 07 Oct 2018 21:29:58: 43000000 INFO @ Sun, 07 Oct 2018 21:29:59: 45000000 INFO @ Sun, 07 Oct 2018 21:30:05: 44000000 INFO @ Sun, 07 Oct 2018 21:30:05: 44000000 INFO @ Sun, 07 Oct 2018 21:30:06: 46000000 INFO @ Sun, 07 Oct 2018 21:30:12: 45000000 INFO @ Sun, 07 Oct 2018 21:30:13: 45000000 INFO @ Sun, 07 Oct 2018 21:30:14: 47000000 INFO @ Sun, 07 Oct 2018 21:30:19: 46000000 INFO @ Sun, 07 Oct 2018 21:30:20: 46000000 INFO @ Sun, 07 Oct 2018 21:30:21: 48000000 INFO @ Sun, 07 Oct 2018 21:30:26: 47000000 INFO @ Sun, 07 Oct 2018 21:30:27: 47000000 INFO @ Sun, 07 Oct 2018 21:30:29: 49000000 INFO @ Sun, 07 Oct 2018 21:30:33: 48000000 INFO @ Sun, 07 Oct 2018 21:30:35: 48000000 INFO @ Sun, 07 Oct 2018 21:30:36: 50000000 INFO @ Sun, 07 Oct 2018 21:30:40: 49000000 INFO @ Sun, 07 Oct 2018 21:30:42: 49000000 INFO @ Sun, 07 Oct 2018 21:30:43: 51000000 INFO @ Sun, 07 Oct 2018 21:30:47: 50000000 INFO @ Sun, 07 Oct 2018 21:30:50: 50000000 INFO @ Sun, 07 Oct 2018 21:30:51: 52000000 INFO @ Sun, 07 Oct 2018 21:30:54: 51000000 INFO @ Sun, 07 Oct 2018 21:30:57: 51000000 INFO @ Sun, 07 Oct 2018 21:30:58: 53000000 INFO @ Sun, 07 Oct 2018 21:31:01: 52000000 INFO @ Sun, 07 Oct 2018 21:31:04: 52000000 INFO @ Sun, 07 Oct 2018 21:31:05: 54000000 INFO @ Sun, 07 Oct 2018 21:31:08: 53000000 INFO @ Sun, 07 Oct 2018 21:31:12: 53000000 INFO @ Sun, 07 Oct 2018 21:31:13: 55000000 INFO @ Sun, 07 Oct 2018 21:31:15: 54000000 INFO @ Sun, 07 Oct 2018 21:31:19: 54000000 INFO @ Sun, 07 Oct 2018 21:31:20: 56000000 INFO @ Sun, 07 Oct 2018 21:31:21: 55000000 INFO @ Sun, 07 Oct 2018 21:31:26: 55000000 INFO @ Sun, 07 Oct 2018 21:31:27: 57000000 INFO @ Sun, 07 Oct 2018 21:31:28: 56000000 INFO @ Sun, 07 Oct 2018 21:31:34: 56000000 INFO @ Sun, 07 Oct 2018 21:31:34: 58000000 INFO @ Sun, 07 Oct 2018 21:31:35: 57000000 INFO @ Sun, 07 Oct 2018 21:31:41: 57000000 INFO @ Sun, 07 Oct 2018 21:31:42: 59000000 INFO @ Sun, 07 Oct 2018 21:31:42: 58000000 INFO @ Sun, 07 Oct 2018 21:31:44: #1 tag size is determined as 51 bps INFO @ Sun, 07 Oct 2018 21:31:44: #1 tag size = 51 INFO @ Sun, 07 Oct 2018 21:31:44: #1 total tags in treatment: 59326131 INFO @ Sun, 07 Oct 2018 21:31:44: #1 user defined the maximum tags... INFO @ Sun, 07 Oct 2018 21:31:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 07 Oct 2018 21:31:46: #1 tags after filtering in treatment: 59326131 INFO @ Sun, 07 Oct 2018 21:31:46: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 07 Oct 2018 21:31:46: #1 finished! INFO @ Sun, 07 Oct 2018 21:31:46: #2 Build Peak Model... INFO @ Sun, 07 Oct 2018 21:31:46: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 07 Oct 2018 21:31:48: 58000000 INFO @ Sun, 07 Oct 2018 21:31:49: 59000000 INFO @ Sun, 07 Oct 2018 21:31:49: #2 number of paired peaks: 0 WARNING @ Sun, 07 Oct 2018 21:31:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 07 Oct 2018 21:31:49: Process for pairing-model is terminated! cat: SRX3630331.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3630331.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3630331.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3630331.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sun, 07 Oct 2018 21:31:52: #1 tag size is determined as 51 bps INFO @ Sun, 07 Oct 2018 21:31:52: #1 tag size = 51 INFO @ Sun, 07 Oct 2018 21:31:52: #1 total tags in treatment: 59326131 INFO @ Sun, 07 Oct 2018 21:31:52: #1 user defined the maximum tags... INFO @ Sun, 07 Oct 2018 21:31:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 07 Oct 2018 21:31:53: #1 tags after filtering in treatment: 59326131 INFO @ Sun, 07 Oct 2018 21:31:53: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 07 Oct 2018 21:31:53: #1 finished! INFO @ Sun, 07 Oct 2018 21:31:53: #2 Build Peak Model... INFO @ Sun, 07 Oct 2018 21:31:53: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 07 Oct 2018 21:31:54: 59000000 INFO @ Sun, 07 Oct 2018 21:31:57: #2 number of paired peaks: 0 WARNING @ Sun, 07 Oct 2018 21:31:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 07 Oct 2018 21:31:57: Process for pairing-model is terminated! cat: SRX3630331.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3630331.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3630331.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3630331.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sun, 07 Oct 2018 21:31:57: #1 tag size is determined as 51 bps INFO @ Sun, 07 Oct 2018 21:31:57: #1 tag size = 51 INFO @ Sun, 07 Oct 2018 21:31:57: #1 total tags in treatment: 59326131 INFO @ Sun, 07 Oct 2018 21:31:57: #1 user defined the maximum tags... INFO @ Sun, 07 Oct 2018 21:31:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 07 Oct 2018 21:31:58: #1 tags after filtering in treatment: 59326131 INFO @ Sun, 07 Oct 2018 21:31:58: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 07 Oct 2018 21:31:58: #1 finished! INFO @ Sun, 07 Oct 2018 21:31:58: #2 Build Peak Model... INFO @ Sun, 07 Oct 2018 21:31:58: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 07 Oct 2018 21:32:02: #2 number of paired peaks: 0 WARNING @ Sun, 07 Oct 2018 21:32:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 07 Oct 2018 21:32:02: Process for pairing-model is terminated! cat: SRX3630331.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3630331.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3630331.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3630331.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。