Job ID = 11240716 sra ファイルのダウンロード中... Completed: 1451043K bytes transferred in 71 seconds (165614K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 71263728 spots for /home/okishinya/chipatlas/results/dm3/SRX3630325/SRR6652847.sra Written 71263728 spots for /home/okishinya/chipatlas/results/dm3/SRX3630325/SRR6652847.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:27:55 71263728 reads; of these: 71263728 (100.00%) were unpaired; of these: 4744135 (6.66%) aligned 0 times 49776373 (69.85%) aligned exactly 1 time 16743220 (23.49%) aligned >1 times 93.34% overall alignment rate Time searching: 00:27:55 Overall time: 00:27:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 13574149 / 66519593 = 0.2041 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 07 Oct 2018 21:18:51: # Command line: callpeak -t SRX3630325.bam -f BAM -g dm -n SRX3630325.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3630325.05 # format = BAM # ChIP-seq file = ['SRX3630325.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 07 Oct 2018 21:18:51: # Command line: callpeak -t SRX3630325.bam -f BAM -g dm -n SRX3630325.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3630325.20 # format = BAM # ChIP-seq file = ['SRX3630325.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 07 Oct 2018 21:18:51: # Command line: callpeak -t SRX3630325.bam -f BAM -g dm -n SRX3630325.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3630325.10 # format = BAM # ChIP-seq file = ['SRX3630325.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 07 Oct 2018 21:18:51: #1 read tag files... INFO @ Sun, 07 Oct 2018 21:18:51: #1 read tag files... INFO @ Sun, 07 Oct 2018 21:18:51: #1 read tag files... INFO @ Sun, 07 Oct 2018 21:18:51: #1 read treatment tags... INFO @ Sun, 07 Oct 2018 21:18:51: #1 read treatment tags... INFO @ Sun, 07 Oct 2018 21:18:51: #1 read treatment tags... INFO @ Sun, 07 Oct 2018 21:18:58: 1000000 INFO @ Sun, 07 Oct 2018 21:18:58: 1000000 INFO @ Sun, 07 Oct 2018 21:18:58: 1000000 INFO @ Sun, 07 Oct 2018 21:19:04: 2000000 INFO @ Sun, 07 Oct 2018 21:19:05: 2000000 INFO @ Sun, 07 Oct 2018 21:19:05: 2000000 INFO @ Sun, 07 Oct 2018 21:19:11: 3000000 INFO @ Sun, 07 Oct 2018 21:19:12: 3000000 INFO @ Sun, 07 Oct 2018 21:19:12: 3000000 INFO @ Sun, 07 Oct 2018 21:19:18: 4000000 INFO @ Sun, 07 Oct 2018 21:19:18: 4000000 INFO @ Sun, 07 Oct 2018 21:19:19: 4000000 INFO @ Sun, 07 Oct 2018 21:19:24: 5000000 INFO @ Sun, 07 Oct 2018 21:19:25: 5000000 INFO @ Sun, 07 Oct 2018 21:19:26: 5000000 INFO @ Sun, 07 Oct 2018 21:19:31: 6000000 INFO @ Sun, 07 Oct 2018 21:19:32: 6000000 INFO @ Sun, 07 Oct 2018 21:19:33: 6000000 INFO @ Sun, 07 Oct 2018 21:19:37: 7000000 INFO @ Sun, 07 Oct 2018 21:19:39: 7000000 INFO @ Sun, 07 Oct 2018 21:19:40: 7000000 INFO @ Sun, 07 Oct 2018 21:19:44: 8000000 INFO @ Sun, 07 Oct 2018 21:19:46: 8000000 INFO @ Sun, 07 Oct 2018 21:19:48: 8000000 INFO @ Sun, 07 Oct 2018 21:19:50: 9000000 INFO @ Sun, 07 Oct 2018 21:19:53: 9000000 INFO @ Sun, 07 Oct 2018 21:19:55: 9000000 INFO @ Sun, 07 Oct 2018 21:19:57: 10000000 INFO @ Sun, 07 Oct 2018 21:20:00: 10000000 INFO @ Sun, 07 Oct 2018 21:20:02: 10000000 INFO @ Sun, 07 Oct 2018 21:20:03: 11000000 INFO @ Sun, 07 Oct 2018 21:20:07: 11000000 INFO @ Sun, 07 Oct 2018 21:20:09: 11000000 INFO @ Sun, 07 Oct 2018 21:20:10: 12000000 INFO @ Sun, 07 Oct 2018 21:20:14: 12000000 INFO @ Sun, 07 Oct 2018 21:20:16: 12000000 INFO @ Sun, 07 Oct 2018 21:20:17: 13000000 INFO @ Sun, 07 Oct 2018 21:20:21: 13000000 INFO @ Sun, 07 Oct 2018 21:20:23: 14000000 INFO @ Sun, 07 Oct 2018 21:20:24: 13000000 INFO @ Sun, 07 Oct 2018 21:20:28: 14000000 INFO @ Sun, 07 Oct 2018 21:20:30: 15000000 INFO @ Sun, 07 Oct 2018 21:20:31: 14000000 INFO @ Sun, 07 Oct 2018 21:20:35: 15000000 INFO @ Sun, 07 Oct 2018 21:20:36: 16000000 INFO @ Sun, 07 Oct 2018 21:20:38: 15000000 INFO @ Sun, 07 Oct 2018 21:20:42: 16000000 INFO @ Sun, 07 Oct 2018 21:20:43: 17000000 INFO @ Sun, 07 Oct 2018 21:20:45: 16000000 INFO @ Sun, 07 Oct 2018 21:20:49: 18000000 INFO @ Sun, 07 Oct 2018 21:20:49: 17000000 INFO @ Sun, 07 Oct 2018 21:20:53: 17000000 INFO @ Sun, 07 Oct 2018 21:20:56: 19000000 INFO @ Sun, 07 Oct 2018 21:20:57: 18000000 INFO @ Sun, 07 Oct 2018 21:21:00: 18000000 INFO @ Sun, 07 Oct 2018 21:21:02: 20000000 INFO @ Sun, 07 Oct 2018 21:21:04: 19000000 INFO @ Sun, 07 Oct 2018 21:21:07: 19000000 INFO @ Sun, 07 Oct 2018 21:21:09: 21000000 INFO @ Sun, 07 Oct 2018 21:21:11: 20000000 INFO @ Sun, 07 Oct 2018 21:21:15: 20000000 INFO @ Sun, 07 Oct 2018 21:21:15: 22000000 INFO @ Sun, 07 Oct 2018 21:21:18: 21000000 INFO @ Sun, 07 Oct 2018 21:21:22: 23000000 INFO @ Sun, 07 Oct 2018 21:21:22: 21000000 INFO @ Sun, 07 Oct 2018 21:21:25: 22000000 INFO @ Sun, 07 Oct 2018 21:21:28: 24000000 INFO @ Sun, 07 Oct 2018 21:21:29: 22000000 INFO @ Sun, 07 Oct 2018 21:21:32: 23000000 INFO @ Sun, 07 Oct 2018 21:21:35: 25000000 INFO @ Sun, 07 Oct 2018 21:21:37: 23000000 INFO @ Sun, 07 Oct 2018 21:21:39: 24000000 INFO @ Sun, 07 Oct 2018 21:21:41: 26000000 INFO @ Sun, 07 Oct 2018 21:21:44: 24000000 INFO @ Sun, 07 Oct 2018 21:21:46: 25000000 INFO @ Sun, 07 Oct 2018 21:21:48: 27000000 INFO @ Sun, 07 Oct 2018 21:21:51: 25000000 INFO @ Sun, 07 Oct 2018 21:21:53: 26000000 INFO @ Sun, 07 Oct 2018 21:21:54: 28000000 INFO @ Sun, 07 Oct 2018 21:21:58: 26000000 INFO @ Sun, 07 Oct 2018 21:21:59: 27000000 INFO @ Sun, 07 Oct 2018 21:22:01: 29000000 INFO @ Sun, 07 Oct 2018 21:22:05: 27000000 INFO @ Sun, 07 Oct 2018 21:22:06: 28000000 INFO @ Sun, 07 Oct 2018 21:22:07: 30000000 INFO @ Sun, 07 Oct 2018 21:22:12: 28000000 INFO @ Sun, 07 Oct 2018 21:22:13: 29000000 INFO @ Sun, 07 Oct 2018 21:22:14: 31000000 INFO @ Sun, 07 Oct 2018 21:22:19: 29000000 INFO @ Sun, 07 Oct 2018 21:22:20: 30000000 INFO @ Sun, 07 Oct 2018 21:22:21: 32000000 INFO @ Sun, 07 Oct 2018 21:22:26: 30000000 INFO @ Sun, 07 Oct 2018 21:22:26: 31000000 INFO @ Sun, 07 Oct 2018 21:22:27: 33000000 INFO @ Sun, 07 Oct 2018 21:22:33: 31000000 INFO @ Sun, 07 Oct 2018 21:22:33: 32000000 INFO @ Sun, 07 Oct 2018 21:22:34: 34000000 INFO @ Sun, 07 Oct 2018 21:22:40: 32000000 INFO @ Sun, 07 Oct 2018 21:22:40: 33000000 INFO @ Sun, 07 Oct 2018 21:22:40: 35000000 INFO @ Sun, 07 Oct 2018 21:22:47: 36000000 INFO @ Sun, 07 Oct 2018 21:22:47: 34000000 INFO @ Sun, 07 Oct 2018 21:22:47: 33000000 INFO @ Sun, 07 Oct 2018 21:22:53: 37000000 INFO @ Sun, 07 Oct 2018 21:22:54: 35000000 INFO @ Sun, 07 Oct 2018 21:22:54: 34000000 INFO @ Sun, 07 Oct 2018 21:23:00: 38000000 INFO @ Sun, 07 Oct 2018 21:23:01: 36000000 INFO @ Sun, 07 Oct 2018 21:23:01: 35000000 INFO @ Sun, 07 Oct 2018 21:23:06: 39000000 INFO @ Sun, 07 Oct 2018 21:23:08: 37000000 INFO @ Sun, 07 Oct 2018 21:23:09: 36000000 INFO @ Sun, 07 Oct 2018 21:23:13: 40000000 INFO @ Sun, 07 Oct 2018 21:23:15: 38000000 INFO @ Sun, 07 Oct 2018 21:23:16: 37000000 INFO @ Sun, 07 Oct 2018 21:23:19: 41000000 INFO @ Sun, 07 Oct 2018 21:23:22: 39000000 INFO @ Sun, 07 Oct 2018 21:23:23: 38000000 INFO @ Sun, 07 Oct 2018 21:23:26: 42000000 INFO @ Sun, 07 Oct 2018 21:23:29: 40000000 INFO @ Sun, 07 Oct 2018 21:23:30: 39000000 INFO @ Sun, 07 Oct 2018 21:23:32: 43000000 INFO @ Sun, 07 Oct 2018 21:23:35: 41000000 INFO @ Sun, 07 Oct 2018 21:23:37: 40000000 INFO @ Sun, 07 Oct 2018 21:23:39: 44000000 INFO @ Sun, 07 Oct 2018 21:23:42: 42000000 INFO @ Sun, 07 Oct 2018 21:23:44: 41000000 INFO @ Sun, 07 Oct 2018 21:23:46: 45000000 INFO @ Sun, 07 Oct 2018 21:23:49: 43000000 INFO @ Sun, 07 Oct 2018 21:23:52: 42000000 INFO @ Sun, 07 Oct 2018 21:23:52: 46000000 INFO @ Sun, 07 Oct 2018 21:23:56: 44000000 INFO @ Sun, 07 Oct 2018 21:23:59: 47000000 INFO @ Sun, 07 Oct 2018 21:23:59: 43000000 INFO @ Sun, 07 Oct 2018 21:24:03: 45000000 INFO @ Sun, 07 Oct 2018 21:24:05: 48000000 INFO @ Sun, 07 Oct 2018 21:24:06: 44000000 INFO @ Sun, 07 Oct 2018 21:24:10: 46000000 INFO @ Sun, 07 Oct 2018 21:24:11: 49000000 INFO @ Sun, 07 Oct 2018 21:24:13: 45000000 INFO @ Sun, 07 Oct 2018 21:24:17: 47000000 INFO @ Sun, 07 Oct 2018 21:24:18: 50000000 INFO @ Sun, 07 Oct 2018 21:24:21: 46000000 INFO @ Sun, 07 Oct 2018 21:24:24: 48000000 INFO @ Sun, 07 Oct 2018 21:24:25: 51000000 INFO @ Sun, 07 Oct 2018 21:24:27: 47000000 INFO @ Sun, 07 Oct 2018 21:24:31: 49000000 INFO @ Sun, 07 Oct 2018 21:24:31: 52000000 INFO @ Sun, 07 Oct 2018 21:24:34: 48000000 INFO @ Sun, 07 Oct 2018 21:24:38: 50000000 INFO @ Sun, 07 Oct 2018 21:24:38: #1 tag size is determined as 51 bps INFO @ Sun, 07 Oct 2018 21:24:38: #1 tag size = 51 INFO @ Sun, 07 Oct 2018 21:24:38: #1 total tags in treatment: 52945444 INFO @ Sun, 07 Oct 2018 21:24:38: #1 user defined the maximum tags... INFO @ Sun, 07 Oct 2018 21:24:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 07 Oct 2018 21:24:39: #1 tags after filtering in treatment: 52945444 INFO @ Sun, 07 Oct 2018 21:24:39: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 07 Oct 2018 21:24:39: #1 finished! INFO @ Sun, 07 Oct 2018 21:24:39: #2 Build Peak Model... INFO @ Sun, 07 Oct 2018 21:24:39: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 07 Oct 2018 21:24:41: 49000000 INFO @ Sun, 07 Oct 2018 21:24:43: #2 number of paired peaks: 0 WARNING @ Sun, 07 Oct 2018 21:24:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 07 Oct 2018 21:24:43: Process for pairing-model is terminated! cat: SRX3630325.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3630325.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3630325.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3630325.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sun, 07 Oct 2018 21:24:44: 51000000 INFO @ Sun, 07 Oct 2018 21:24:47: 50000000 INFO @ Sun, 07 Oct 2018 21:24:51: 52000000 INFO @ Sun, 07 Oct 2018 21:24:54: 51000000 INFO @ Sun, 07 Oct 2018 21:24:57: #1 tag size is determined as 51 bps INFO @ Sun, 07 Oct 2018 21:24:57: #1 tag size = 51 INFO @ Sun, 07 Oct 2018 21:24:57: #1 total tags in treatment: 52945444 INFO @ Sun, 07 Oct 2018 21:24:57: #1 user defined the maximum tags... INFO @ Sun, 07 Oct 2018 21:24:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 07 Oct 2018 21:24:58: #1 tags after filtering in treatment: 52945444 INFO @ Sun, 07 Oct 2018 21:24:58: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 07 Oct 2018 21:24:58: #1 finished! INFO @ Sun, 07 Oct 2018 21:24:58: #2 Build Peak Model... INFO @ Sun, 07 Oct 2018 21:24:58: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 07 Oct 2018 21:25:01: 52000000 INFO @ Sun, 07 Oct 2018 21:25:01: #2 number of paired peaks: 0 WARNING @ Sun, 07 Oct 2018 21:25:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 07 Oct 2018 21:25:01: Process for pairing-model is terminated! cat: SRX3630325.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3630325.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3630325.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3630325.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sun, 07 Oct 2018 21:25:07: #1 tag size is determined as 51 bps INFO @ Sun, 07 Oct 2018 21:25:07: #1 tag size = 51 INFO @ Sun, 07 Oct 2018 21:25:07: #1 total tags in treatment: 52945444 INFO @ Sun, 07 Oct 2018 21:25:07: #1 user defined the maximum tags... INFO @ Sun, 07 Oct 2018 21:25:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 07 Oct 2018 21:25:09: #1 tags after filtering in treatment: 52945444 INFO @ Sun, 07 Oct 2018 21:25:09: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 07 Oct 2018 21:25:09: #1 finished! INFO @ Sun, 07 Oct 2018 21:25:09: #2 Build Peak Model... INFO @ Sun, 07 Oct 2018 21:25:09: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 07 Oct 2018 21:25:12: #2 number of paired peaks: 0 WARNING @ Sun, 07 Oct 2018 21:25:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 07 Oct 2018 21:25:12: Process for pairing-model is terminated! cat: SRX3630325.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3630325.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3630325.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3630325.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。