Job ID = 1295481 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2019-06-03T06:12:39 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 26,494,398 reads read : 52,988,796 reads written : 52,988,796 2019-06-03T07:15:49 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-03T07:17:08 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-03T08:17:53 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-03T08:49:57 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-06-03T08:56:33 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 24,529,813 reads read : 49,059,626 reads written : 49,059,626 rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 02:44:19 51024211 reads; of these: 51024211 (100.00%) were paired; of these: 15290933 (29.97%) aligned concordantly 0 times 26538226 (52.01%) aligned concordantly exactly 1 time 9195052 (18.02%) aligned concordantly >1 times ---- 15290933 pairs aligned concordantly 0 times; of these: 5616899 (36.73%) aligned discordantly 1 time ---- 9674034 pairs aligned 0 times concordantly or discordantly; of these: 19348068 mates make up the pairs; of these: 14810553 (76.55%) aligned 0 times 890487 (4.60%) aligned exactly 1 time 3647028 (18.85%) aligned >1 times 85.49% overall alignment rate Time searching: 02:44:19 Overall time: 02:44:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 56 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 18728180 / 40891810 = 0.4580 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 21:50:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3404056/SRX3404056.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3404056/SRX3404056.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3404056/SRX3404056.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3404056/SRX3404056.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:50:40: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:50:40: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:50:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3404056/SRX3404056.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3404056/SRX3404056.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3404056/SRX3404056.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3404056/SRX3404056.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:50:40: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:50:40: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:50:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX3404056/SRX3404056.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX3404056/SRX3404056.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX3404056/SRX3404056.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX3404056/SRX3404056.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 21:50:40: #1 read tag files... INFO @ Mon, 03 Jun 2019 21:50:40: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 21:50:55: 1000000 INFO @ Mon, 03 Jun 2019 21:50:57: 1000000 INFO @ Mon, 03 Jun 2019 21:50:58: 1000000 INFO @ Mon, 03 Jun 2019 21:51:10: 2000000 INFO @ Mon, 03 Jun 2019 21:51:11: 2000000 INFO @ Mon, 03 Jun 2019 21:51:16: 2000000 INFO @ Mon, 03 Jun 2019 21:51:24: 3000000 INFO @ Mon, 03 Jun 2019 21:51:25: 3000000 INFO @ Mon, 03 Jun 2019 21:51:34: 3000000 INFO @ Mon, 03 Jun 2019 21:51:38: 4000000 INFO @ Mon, 03 Jun 2019 21:51:39: 4000000 INFO @ Mon, 03 Jun 2019 21:51:52: 4000000 INFO @ Mon, 03 Jun 2019 21:51:53: 5000000 INFO @ Mon, 03 Jun 2019 21:51:54: 5000000 INFO @ Mon, 03 Jun 2019 21:52:06: 6000000 INFO @ Mon, 03 Jun 2019 21:52:09: 6000000 INFO @ Mon, 03 Jun 2019 21:52:09: 5000000 INFO @ Mon, 03 Jun 2019 21:52:18: 7000000 INFO @ Mon, 03 Jun 2019 21:52:22: 7000000 INFO @ Mon, 03 Jun 2019 21:52:26: 6000000 INFO @ Mon, 03 Jun 2019 21:52:31: 8000000 INFO @ Mon, 03 Jun 2019 21:52:36: 8000000 INFO @ Mon, 03 Jun 2019 21:52:43: 7000000 INFO @ Mon, 03 Jun 2019 21:52:43: 9000000 INFO @ Mon, 03 Jun 2019 21:52:50: 9000000 INFO @ Mon, 03 Jun 2019 21:52:56: 10000000 INFO @ Mon, 03 Jun 2019 21:53:00: 8000000 INFO @ Mon, 03 Jun 2019 21:53:04: 10000000 INFO @ Mon, 03 Jun 2019 21:53:09: 11000000 INFO @ Mon, 03 Jun 2019 21:53:16: 9000000 INFO @ Mon, 03 Jun 2019 21:53:18: 11000000 INFO @ Mon, 03 Jun 2019 21:53:21: 12000000 INFO @ Mon, 03 Jun 2019 21:53:32: 12000000 INFO @ Mon, 03 Jun 2019 21:53:33: 10000000 INFO @ Mon, 03 Jun 2019 21:53:34: 13000000 INFO @ Mon, 03 Jun 2019 21:53:46: 13000000 INFO @ Mon, 03 Jun 2019 21:53:47: 14000000 INFO @ Mon, 03 Jun 2019 21:53:49: 11000000 INFO @ Mon, 03 Jun 2019 21:53:59: 15000000 INFO @ Mon, 03 Jun 2019 21:54:00: 14000000 INFO @ Mon, 03 Jun 2019 21:54:05: 12000000 INFO @ Mon, 03 Jun 2019 21:54:12: 16000000 INFO @ Mon, 03 Jun 2019 21:54:14: 15000000 INFO @ Mon, 03 Jun 2019 21:54:21: 13000000 INFO @ Mon, 03 Jun 2019 21:54:24: 17000000 INFO @ Mon, 03 Jun 2019 21:54:27: 16000000 INFO @ Mon, 03 Jun 2019 21:54:37: 14000000 INFO @ Mon, 03 Jun 2019 21:54:37: 18000000 INFO @ Mon, 03 Jun 2019 21:54:41: 17000000 INFO @ Mon, 03 Jun 2019 21:54:50: 19000000 INFO @ Mon, 03 Jun 2019 21:54:53: 15000000 INFO @ Mon, 03 Jun 2019 21:54:55: 18000000 INFO @ Mon, 03 Jun 2019 21:55:03: 20000000 INFO @ Mon, 03 Jun 2019 21:55:09: 16000000 INFO @ Mon, 03 Jun 2019 21:55:09: 19000000 INFO @ Mon, 03 Jun 2019 21:55:16: 21000000 INFO @ Mon, 03 Jun 2019 21:55:22: 20000000 INFO @ Mon, 03 Jun 2019 21:55:24: 17000000 INFO @ Mon, 03 Jun 2019 21:55:30: 22000000 INFO @ Mon, 03 Jun 2019 21:55:36: 21000000 INFO @ Mon, 03 Jun 2019 21:55:40: 18000000 INFO @ Mon, 03 Jun 2019 21:55:43: 23000000 INFO @ Mon, 03 Jun 2019 21:55:50: 22000000 INFO @ Mon, 03 Jun 2019 21:55:55: 19000000 INFO @ Mon, 03 Jun 2019 21:55:57: 24000000 INFO @ Mon, 03 Jun 2019 21:56:04: 23000000 INFO @ Mon, 03 Jun 2019 21:56:10: 25000000 INFO @ Mon, 03 Jun 2019 21:56:11: 20000000 INFO @ Mon, 03 Jun 2019 21:56:18: 24000000 INFO @ Mon, 03 Jun 2019 21:56:23: 26000000 INFO @ Mon, 03 Jun 2019 21:56:26: 21000000 INFO @ Mon, 03 Jun 2019 21:56:32: 25000000 INFO @ Mon, 03 Jun 2019 21:56:36: 27000000 INFO @ Mon, 03 Jun 2019 21:56:42: 22000000 INFO @ Mon, 03 Jun 2019 21:56:46: 26000000 INFO @ Mon, 03 Jun 2019 21:56:49: 28000000 INFO @ Mon, 03 Jun 2019 21:56:59: 23000000 INFO @ Mon, 03 Jun 2019 21:57:00: 27000000 INFO @ Mon, 03 Jun 2019 21:57:02: 29000000 INFO @ Mon, 03 Jun 2019 21:57:13: 28000000 INFO @ Mon, 03 Jun 2019 21:57:15: 24000000 INFO @ Mon, 03 Jun 2019 21:57:15: 30000000 INFO @ Mon, 03 Jun 2019 21:57:27: 29000000 INFO @ Mon, 03 Jun 2019 21:57:28: 31000000 INFO @ Mon, 03 Jun 2019 21:57:31: 25000000 INFO @ Mon, 03 Jun 2019 21:57:41: 30000000 INFO @ Mon, 03 Jun 2019 21:57:41: 32000000 INFO @ Mon, 03 Jun 2019 21:57:46: 26000000 INFO @ Mon, 03 Jun 2019 21:57:54: 33000000 INFO @ Mon, 03 Jun 2019 21:57:54: 31000000 INFO @ Mon, 03 Jun 2019 21:58:02: 27000000 INFO @ Mon, 03 Jun 2019 21:58:06: 34000000 INFO @ Mon, 03 Jun 2019 21:58:07: 32000000 INFO @ Mon, 03 Jun 2019 21:58:18: 28000000 INFO @ Mon, 03 Jun 2019 21:58:18: 35000000 INFO @ Mon, 03 Jun 2019 21:58:21: 33000000 INFO @ Mon, 03 Jun 2019 21:58:32: 36000000 INFO @ Mon, 03 Jun 2019 21:58:34: 29000000 INFO @ Mon, 03 Jun 2019 21:58:34: 34000000 INFO @ Mon, 03 Jun 2019 21:58:44: 37000000 INFO @ Mon, 03 Jun 2019 21:58:47: 35000000 INFO @ Mon, 03 Jun 2019 21:58:49: 30000000 INFO @ Mon, 03 Jun 2019 21:58:57: 38000000 INFO @ Mon, 03 Jun 2019 21:59:01: 36000000 INFO @ Mon, 03 Jun 2019 21:59:04: 31000000 INFO @ Mon, 03 Jun 2019 21:59:10: 39000000 INFO @ Mon, 03 Jun 2019 21:59:14: 37000000 INFO @ Mon, 03 Jun 2019 21:59:19: 32000000 INFO @ Mon, 03 Jun 2019 21:59:23: 40000000 INFO @ Mon, 03 Jun 2019 21:59:27: 38000000 INFO @ Mon, 03 Jun 2019 21:59:35: 33000000 INFO @ Mon, 03 Jun 2019 21:59:36: 41000000 INFO @ Mon, 03 Jun 2019 21:59:41: 39000000 INFO @ Mon, 03 Jun 2019 21:59:49: 42000000 INFO @ Mon, 03 Jun 2019 21:59:49: 34000000 INFO @ Mon, 03 Jun 2019 21:59:54: 40000000 INFO @ Mon, 03 Jun 2019 22:00:02: 43000000 INFO @ Mon, 03 Jun 2019 22:00:04: 35000000 INFO @ Mon, 03 Jun 2019 22:00:07: 41000000 INFO @ Mon, 03 Jun 2019 22:00:16: 44000000 INFO @ Mon, 03 Jun 2019 22:00:19: 36000000 INFO @ Mon, 03 Jun 2019 22:00:20: 42000000 INFO @ Mon, 03 Jun 2019 22:00:28: 45000000 INFO @ Mon, 03 Jun 2019 22:00:33: 43000000 INFO @ Mon, 03 Jun 2019 22:00:34: 37000000 INFO @ Mon, 03 Jun 2019 22:00:42: 46000000 INFO @ Mon, 03 Jun 2019 22:00:47: 44000000 INFO @ Mon, 03 Jun 2019 22:00:49: 38000000 INFO @ Mon, 03 Jun 2019 22:00:54: 47000000 INFO @ Mon, 03 Jun 2019 22:01:00: 45000000 INFO @ Mon, 03 Jun 2019 22:01:05: 39000000 INFO @ Mon, 03 Jun 2019 22:01:07: 48000000 INFO @ Mon, 03 Jun 2019 22:01:13: 46000000 INFO @ Mon, 03 Jun 2019 22:01:20: 40000000 INFO @ Mon, 03 Jun 2019 22:01:20: 49000000 INFO @ Mon, 03 Jun 2019 22:01:27: 47000000 INFO @ Mon, 03 Jun 2019 22:01:30: #1 tag size is determined as 119 bps INFO @ Mon, 03 Jun 2019 22:01:30: #1 tag size = 119 INFO @ Mon, 03 Jun 2019 22:01:30: #1 total tags in treatment: 18751270 INFO @ Mon, 03 Jun 2019 22:01:30: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 22:01:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 22:01:31: #1 tags after filtering in treatment: 13994066 INFO @ Mon, 03 Jun 2019 22:01:31: #1 Redundant rate of treatment: 0.25 INFO @ Mon, 03 Jun 2019 22:01:31: #1 finished! INFO @ Mon, 03 Jun 2019 22:01:31: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 22:01:31: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 22:01:32: #2 number of paired peaks: 2408 INFO @ Mon, 03 Jun 2019 22:01:32: start model_add_line... INFO @ Mon, 03 Jun 2019 22:01:33: start X-correlation... INFO @ Mon, 03 Jun 2019 22:01:33: end of X-cor INFO @ Mon, 03 Jun 2019 22:01:33: #2 finished! INFO @ Mon, 03 Jun 2019 22:01:33: #2 predicted fragment length is 126 bps INFO @ Mon, 03 Jun 2019 22:01:33: #2 alternative fragment length(s) may be 126 bps INFO @ Mon, 03 Jun 2019 22:01:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX3404056/SRX3404056.05_model.r WARNING @ Mon, 03 Jun 2019 22:01:33: #2 Since the d (126) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 22:01:33: #2 You may need to consider one of the other alternative d(s): 126 WARNING @ Mon, 03 Jun 2019 22:01:33: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 22:01:33: #3 Call peaks... INFO @ Mon, 03 Jun 2019 22:01:33: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 22:01:35: 41000000 INFO @ Mon, 03 Jun 2019 22:01:40: 48000000 INFO @ Mon, 03 Jun 2019 22:01:50: 42000000 INFO @ Mon, 03 Jun 2019 22:01:53: 49000000 INFO @ Mon, 03 Jun 2019 22:02:03: #1 tag size is determined as 119 bps INFO @ Mon, 03 Jun 2019 22:02:03: #1 tag size = 119 INFO @ Mon, 03 Jun 2019 22:02:03: #1 total tags in treatment: 18751270 INFO @ Mon, 03 Jun 2019 22:02:03: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 22:02:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 22:02:03: #1 tags after filtering in treatment: 13994066 INFO @ Mon, 03 Jun 2019 22:02:03: #1 Redundant rate of treatment: 0.25 INFO @ Mon, 03 Jun 2019 22:02:03: #1 finished! INFO @ Mon, 03 Jun 2019 22:02:03: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 22:02:03: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 22:02:05: #2 number of paired peaks: 2408 INFO @ Mon, 03 Jun 2019 22:02:05: start model_add_line... INFO @ Mon, 03 Jun 2019 22:02:05: start X-correlation... INFO @ Mon, 03 Jun 2019 22:02:05: end of X-cor INFO @ Mon, 03 Jun 2019 22:02:05: #2 finished! INFO @ Mon, 03 Jun 2019 22:02:05: #2 predicted fragment length is 126 bps INFO @ Mon, 03 Jun 2019 22:02:05: #2 alternative fragment length(s) may be 126 bps INFO @ Mon, 03 Jun 2019 22:02:05: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX3404056/SRX3404056.10_model.r WARNING @ Mon, 03 Jun 2019 22:02:05: #2 Since the d (126) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 22:02:05: #2 You may need to consider one of the other alternative d(s): 126 WARNING @ Mon, 03 Jun 2019 22:02:05: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 22:02:05: #3 Call peaks... INFO @ Mon, 03 Jun 2019 22:02:05: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 22:02:06: 43000000 INFO @ Mon, 03 Jun 2019 22:02:19: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 22:02:21: 44000000 INFO @ Mon, 03 Jun 2019 22:02:36: 45000000 INFO @ Mon, 03 Jun 2019 22:02:41: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX3404056/SRX3404056.05_peaks.xls INFO @ Mon, 03 Jun 2019 22:02:42: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX3404056/SRX3404056.05_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 22:02:42: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX3404056/SRX3404056.05_summits.bed INFO @ Mon, 03 Jun 2019 22:02:42: Done! pass1 - making usageList (15 chroms): 5 millis pass2 - checking and writing primary data (13985 records, 4 fields): 22 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 22:02:50: #3 Call peaks for each chromosome... INFO @ Mon, 03 Jun 2019 22:02:51: 46000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 03 Jun 2019 22:03:06: 47000000 INFO @ Mon, 03 Jun 2019 22:03:12: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX3404056/SRX3404056.10_peaks.xls INFO @ Mon, 03 Jun 2019 22:03:12: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX3404056/SRX3404056.10_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 22:03:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX3404056/SRX3404056.10_summits.bed INFO @ Mon, 03 Jun 2019 22:03:13: Done! pass1 - making usageList (15 chroms): 4 millis pass2 - checking and writing primary data (9661 records, 4 fields): 18 millis CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 22:03:21: 48000000 INFO @ Mon, 03 Jun 2019 22:03:37: 49000000 INFO @ Mon, 03 Jun 2019 22:03:48: #1 tag size is determined as 119 bps INFO @ Mon, 03 Jun 2019 22:03:48: #1 tag size = 119 INFO @ Mon, 03 Jun 2019 22:03:48: #1 total tags in treatment: 18751270 INFO @ Mon, 03 Jun 2019 22:03:48: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 22:03:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 22:03:49: #1 tags after filtering in treatment: 13994066 INFO @ Mon, 03 Jun 2019 22:03:49: #1 Redundant rate of treatment: 0.25 INFO @ Mon, 03 Jun 2019 22:03:49: #1 finished! INFO @ Mon, 03 Jun 2019 22:03:49: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 22:03:49: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 22:03:50: #2 number of paired peaks: 2408 INFO @ Mon, 03 Jun 2019 22:03:50: start model_add_line... INFO @ Mon, 03 Jun 2019 22:03:51: start X-correlation... INFO @ Mon, 03 Jun 2019 22:03:51: end of X-cor INFO @ Mon, 03 Jun 2019 22:03:51: #2 finished! INFO @ Mon, 03 Jun 2019 22:03:51: #2 predicted fragment length is 126 bps INFO @ Mon, 03 Jun 2019 22:03:51: #2 alternative fragment length(s) may be 126 bps INFO @ Mon, 03 Jun 2019 22:03:51: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX3404056/SRX3404056.20_model.r WARNING @ Mon, 03 Jun 2019 22:03:51: #2 Since the d (126) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Mon, 03 Jun 2019 22:03:51: #2 You may need to consider one of the other alternative d(s): 126 WARNING @ Mon, 03 Jun 2019 22:03:51: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Mon, 03 Jun 2019 22:03:51: #3 Call peaks... INFO @ Mon, 03 Jun 2019 22:03:51: #3 Pre-compute pvalue-qvalue table... INFO @ Mon, 03 Jun 2019 22:04:36: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Mon, 03 Jun 2019 22:04:58: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX3404056/SRX3404056.20_peaks.xls INFO @ Mon, 03 Jun 2019 22:04:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX3404056/SRX3404056.20_peaks.narrowPeak INFO @ Mon, 03 Jun 2019 22:04:59: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX3404056/SRX3404056.20_summits.bed INFO @ Mon, 03 Jun 2019 22:04:59: Done! pass1 - making usageList (15 chroms): 3 millis pass2 - checking and writing primary data (5338 records, 4 fields): 12 millis CompletedMACS2peakCalling