Job ID = 1295411 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 23,821,087 reads read : 23,821,087 reads written : 23,821,087 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:47 23821087 reads; of these: 23821087 (100.00%) were unpaired; of these: 538794 (2.26%) aligned 0 times 16433451 (68.99%) aligned exactly 1 time 6848842 (28.75%) aligned >1 times 97.74% overall alignment rate Time searching: 00:09:47 Overall time: 00:09:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5749341 / 23282293 = 0.2469 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 13:58:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX336285/SRX336285.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX336285/SRX336285.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX336285/SRX336285.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX336285/SRX336285.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:58:39: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:58:39: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:58:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX336285/SRX336285.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX336285/SRX336285.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX336285/SRX336285.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX336285/SRX336285.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:58:39: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:58:39: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:58:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX336285/SRX336285.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX336285/SRX336285.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX336285/SRX336285.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX336285/SRX336285.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:58:39: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:58:39: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:58:46: 1000000 INFO @ Mon, 03 Jun 2019 13:58:47: 1000000 INFO @ Mon, 03 Jun 2019 13:58:48: 1000000 INFO @ Mon, 03 Jun 2019 13:58:53: 2000000 INFO @ Mon, 03 Jun 2019 13:58:54: 2000000 INFO @ Mon, 03 Jun 2019 13:58:56: 2000000 INFO @ Mon, 03 Jun 2019 13:58:59: 3000000 INFO @ Mon, 03 Jun 2019 13:59:02: 3000000 INFO @ Mon, 03 Jun 2019 13:59:04: 3000000 INFO @ Mon, 03 Jun 2019 13:59:06: 4000000 INFO @ Mon, 03 Jun 2019 13:59:09: 4000000 INFO @ Mon, 03 Jun 2019 13:59:11: 4000000 INFO @ Mon, 03 Jun 2019 13:59:13: 5000000 INFO @ Mon, 03 Jun 2019 13:59:17: 5000000 INFO @ Mon, 03 Jun 2019 13:59:19: 5000000 INFO @ Mon, 03 Jun 2019 13:59:20: 6000000 INFO @ Mon, 03 Jun 2019 13:59:24: 6000000 INFO @ Mon, 03 Jun 2019 13:59:27: 7000000 INFO @ Mon, 03 Jun 2019 13:59:27: 6000000 INFO @ Mon, 03 Jun 2019 13:59:32: 7000000 INFO @ Mon, 03 Jun 2019 13:59:33: 8000000 INFO @ Mon, 03 Jun 2019 13:59:35: 7000000 INFO @ Mon, 03 Jun 2019 13:59:39: 8000000 INFO @ Mon, 03 Jun 2019 13:59:40: 9000000 INFO @ Mon, 03 Jun 2019 13:59:42: 8000000 INFO @ Mon, 03 Jun 2019 13:59:47: 9000000 INFO @ Mon, 03 Jun 2019 13:59:47: 10000000 INFO @ Mon, 03 Jun 2019 13:59:50: 9000000 INFO @ Mon, 03 Jun 2019 13:59:54: 11000000 INFO @ Mon, 03 Jun 2019 13:59:54: 10000000 INFO @ Mon, 03 Jun 2019 13:59:58: 10000000 INFO @ Mon, 03 Jun 2019 14:00:01: 12000000 INFO @ Mon, 03 Jun 2019 14:00:02: 11000000 INFO @ Mon, 03 Jun 2019 14:00:05: 11000000 INFO @ Mon, 03 Jun 2019 14:00:08: 13000000 INFO @ Mon, 03 Jun 2019 14:00:09: 12000000 INFO @ Mon, 03 Jun 2019 14:00:13: 12000000 INFO @ Mon, 03 Jun 2019 14:00:14: 14000000 INFO @ Mon, 03 Jun 2019 14:00:17: 13000000 INFO @ Mon, 03 Jun 2019 14:00:21: 15000000 INFO @ Mon, 03 Jun 2019 14:00:21: 13000000 INFO @ Mon, 03 Jun 2019 14:00:24: 14000000 INFO @ Mon, 03 Jun 2019 14:00:28: 16000000 INFO @ Mon, 03 Jun 2019 14:00:29: 14000000 INFO @ Mon, 03 Jun 2019 14:00:32: 15000000 INFO @ Mon, 03 Jun 2019 14:00:35: 17000000 INFO @ Mon, 03 Jun 2019 14:00:36: 15000000 INFO @ Mon, 03 Jun 2019 14:00:39: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 14:00:39: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 14:00:39: #1 total tags in treatment: 17532952 INFO @ Mon, 03 Jun 2019 14:00:39: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 14:00:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 14:00:39: #1 tags after filtering in treatment: 17532952 INFO @ Mon, 03 Jun 2019 14:00:39: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 14:00:39: #1 finished! INFO @ Mon, 03 Jun 2019 14:00:39: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 14:00:39: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 14:00:40: 16000000 INFO @ Mon, 03 Jun 2019 14:00:41: #2 number of paired peaks: 6 WARNING @ Mon, 03 Jun 2019 14:00:41: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 14:00:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX336285/SRX336285.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX336285/SRX336285.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX336285/SRX336285.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX336285/SRX336285.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 14:00:44: 16000000 INFO @ Mon, 03 Jun 2019 14:00:47: 17000000 INFO @ Mon, 03 Jun 2019 14:00:52: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 14:00:52: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 14:00:52: #1 total tags in treatment: 17532952 INFO @ Mon, 03 Jun 2019 14:00:52: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 14:00:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 14:00:52: 17000000 INFO @ Mon, 03 Jun 2019 14:00:52: #1 tags after filtering in treatment: 17532952 INFO @ Mon, 03 Jun 2019 14:00:52: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 14:00:52: #1 finished! INFO @ Mon, 03 Jun 2019 14:00:52: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 14:00:52: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 14:00:53: #2 number of paired peaks: 6 WARNING @ Mon, 03 Jun 2019 14:00:53: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 14:00:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX336285/SRX336285.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX336285/SRX336285.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX336285/SRX336285.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX336285/SRX336285.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 14:00:56: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 14:00:56: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 14:00:56: #1 total tags in treatment: 17532952 INFO @ Mon, 03 Jun 2019 14:00:56: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 14:00:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 14:00:56: #1 tags after filtering in treatment: 17532952 INFO @ Mon, 03 Jun 2019 14:00:56: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 14:00:56: #1 finished! INFO @ Mon, 03 Jun 2019 14:00:56: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 14:00:56: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 14:00:57: #2 number of paired peaks: 6 WARNING @ Mon, 03 Jun 2019 14:00:57: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 14:00:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX336285/SRX336285.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX336285/SRX336285.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX336285/SRX336285.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX336285/SRX336285.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。