Job ID = 1295378 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 11,648,528 reads read : 11,648,528 reads written : 11,648,528 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:25 11648528 reads; of these: 11648528 (100.00%) were unpaired; of these: 536088 (4.60%) aligned 0 times 8487856 (72.87%) aligned exactly 1 time 2624584 (22.53%) aligned >1 times 95.40% overall alignment rate Time searching: 00:04:25 Overall time: 00:04:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1764598 / 11112440 = 0.1588 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 13:31:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335512/SRX335512.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335512/SRX335512.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335512/SRX335512.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335512/SRX335512.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:31:46: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:31:46: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:31:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335512/SRX335512.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335512/SRX335512.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335512/SRX335512.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335512/SRX335512.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:31:46: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:31:46: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:31:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335512/SRX335512.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335512/SRX335512.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335512/SRX335512.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335512/SRX335512.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:31:46: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:31:46: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:31:55: 1000000 INFO @ Mon, 03 Jun 2019 13:31:55: 1000000 INFO @ Mon, 03 Jun 2019 13:31:57: 1000000 INFO @ Mon, 03 Jun 2019 13:32:04: 2000000 INFO @ Mon, 03 Jun 2019 13:32:04: 2000000 INFO @ Mon, 03 Jun 2019 13:32:09: 2000000 INFO @ Mon, 03 Jun 2019 13:32:14: 3000000 INFO @ Mon, 03 Jun 2019 13:32:14: 3000000 INFO @ Mon, 03 Jun 2019 13:32:20: 3000000 INFO @ Mon, 03 Jun 2019 13:32:22: 4000000 INFO @ Mon, 03 Jun 2019 13:32:23: 4000000 INFO @ Mon, 03 Jun 2019 13:32:31: 4000000 INFO @ Mon, 03 Jun 2019 13:32:31: 5000000 INFO @ Mon, 03 Jun 2019 13:32:32: 5000000 INFO @ Mon, 03 Jun 2019 13:32:40: 6000000 INFO @ Mon, 03 Jun 2019 13:32:41: 6000000 INFO @ Mon, 03 Jun 2019 13:32:41: 5000000 INFO @ Mon, 03 Jun 2019 13:32:50: 7000000 INFO @ Mon, 03 Jun 2019 13:32:51: 7000000 INFO @ Mon, 03 Jun 2019 13:32:53: 6000000 INFO @ Mon, 03 Jun 2019 13:32:59: 8000000 INFO @ Mon, 03 Jun 2019 13:33:00: 8000000 INFO @ Mon, 03 Jun 2019 13:33:04: 7000000 INFO @ Mon, 03 Jun 2019 13:33:07: 9000000 INFO @ Mon, 03 Jun 2019 13:33:09: 9000000 INFO @ Mon, 03 Jun 2019 13:33:10: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:33:10: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:33:10: #1 total tags in treatment: 9347842 INFO @ Mon, 03 Jun 2019 13:33:10: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:33:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:33:11: #1 tags after filtering in treatment: 9347842 INFO @ Mon, 03 Jun 2019 13:33:11: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:33:11: #1 finished! INFO @ Mon, 03 Jun 2019 13:33:11: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:33:11: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:33:12: #2 number of paired peaks: 46 WARNING @ Mon, 03 Jun 2019 13:33:12: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:33:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX335512/SRX335512.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335512/SRX335512.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335512/SRX335512.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335512/SRX335512.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 13:33:12: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:33:12: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:33:12: #1 total tags in treatment: 9347842 INFO @ Mon, 03 Jun 2019 13:33:12: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:33:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:33:12: #1 tags after filtering in treatment: 9347842 INFO @ Mon, 03 Jun 2019 13:33:12: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:33:12: #1 finished! INFO @ Mon, 03 Jun 2019 13:33:12: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:33:12: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:33:13: #2 number of paired peaks: 46 WARNING @ Mon, 03 Jun 2019 13:33:13: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:33:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX335512/SRX335512.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335512/SRX335512.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335512/SRX335512.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335512/SRX335512.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 13:33:16: 8000000 INFO @ Mon, 03 Jun 2019 13:33:26: 9000000 INFO @ Mon, 03 Jun 2019 13:33:29: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:33:29: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:33:29: #1 total tags in treatment: 9347842 INFO @ Mon, 03 Jun 2019 13:33:29: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:33:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:33:29: #1 tags after filtering in treatment: 9347842 INFO @ Mon, 03 Jun 2019 13:33:29: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:33:29: #1 finished! INFO @ Mon, 03 Jun 2019 13:33:29: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:33:29: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:33:30: #2 number of paired peaks: 46 WARNING @ Mon, 03 Jun 2019 13:33:30: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:33:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX335512/SRX335512.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335512/SRX335512.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335512/SRX335512.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335512/SRX335512.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。