Job ID = 6527947 SRX = SRX335507 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:45:44 prefetch.2.10.7: 1) Downloading 'SRR952849'... 2020-06-29T13:45:44 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:50:05 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:50:05 prefetch.2.10.7: 1) 'SRR952849' was downloaded successfully Read 28429556 spots for SRR952849/SRR952849.sra Written 28429556 spots for SRR952849/SRR952849.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:28 28429556 reads; of these: 28429556 (100.00%) were unpaired; of these: 10256839 (36.08%) aligned 0 times 13954899 (49.09%) aligned exactly 1 time 4217818 (14.84%) aligned >1 times 63.92% overall alignment rate Time searching: 00:06:28 Overall time: 00:06:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3905987 / 18172717 = 0.2149 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:08:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335507/SRX335507.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335507/SRX335507.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335507/SRX335507.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335507/SRX335507.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:08:55: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:08:55: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:09:00: 1000000 INFO @ Mon, 29 Jun 2020 23:09:06: 2000000 INFO @ Mon, 29 Jun 2020 23:09:11: 3000000 INFO @ Mon, 29 Jun 2020 23:09:16: 4000000 INFO @ Mon, 29 Jun 2020 23:09:21: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:09:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335507/SRX335507.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335507/SRX335507.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335507/SRX335507.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335507/SRX335507.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:09:25: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:09:25: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:09:27: 6000000 INFO @ Mon, 29 Jun 2020 23:09:31: 1000000 INFO @ Mon, 29 Jun 2020 23:09:32: 7000000 INFO @ Mon, 29 Jun 2020 23:09:37: 2000000 INFO @ Mon, 29 Jun 2020 23:09:38: 8000000 INFO @ Mon, 29 Jun 2020 23:09:42: 3000000 INFO @ Mon, 29 Jun 2020 23:09:44: 9000000 INFO @ Mon, 29 Jun 2020 23:09:48: 4000000 INFO @ Mon, 29 Jun 2020 23:09:50: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:09:54: 5000000 INFO @ Mon, 29 Jun 2020 23:09:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335507/SRX335507.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335507/SRX335507.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335507/SRX335507.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335507/SRX335507.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:09:55: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:09:55: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:09:56: 11000000 INFO @ Mon, 29 Jun 2020 23:10:00: 6000000 INFO @ Mon, 29 Jun 2020 23:10:01: 1000000 INFO @ Mon, 29 Jun 2020 23:10:01: 12000000 INFO @ Mon, 29 Jun 2020 23:10:06: 7000000 INFO @ Mon, 29 Jun 2020 23:10:07: 2000000 INFO @ Mon, 29 Jun 2020 23:10:07: 13000000 INFO @ Mon, 29 Jun 2020 23:10:12: 8000000 INFO @ Mon, 29 Jun 2020 23:10:13: 3000000 INFO @ Mon, 29 Jun 2020 23:10:13: 14000000 INFO @ Mon, 29 Jun 2020 23:10:15: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:10:15: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:10:15: #1 total tags in treatment: 14266730 INFO @ Mon, 29 Jun 2020 23:10:15: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:10:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:10:15: #1 tags after filtering in treatment: 14266730 INFO @ Mon, 29 Jun 2020 23:10:15: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:10:15: #1 finished! INFO @ Mon, 29 Jun 2020 23:10:15: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:10:15: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:10:16: #2 number of paired peaks: 46 WARNING @ Mon, 29 Jun 2020 23:10:16: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:10:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX335507/SRX335507.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335507/SRX335507.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335507/SRX335507.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335507/SRX335507.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:10:18: 9000000 INFO @ Mon, 29 Jun 2020 23:10:19: 4000000 INFO @ Mon, 29 Jun 2020 23:10:23: 10000000 INFO @ Mon, 29 Jun 2020 23:10:25: 5000000 INFO @ Mon, 29 Jun 2020 23:10:29: 11000000 INFO @ Mon, 29 Jun 2020 23:10:30: 6000000 INFO @ Mon, 29 Jun 2020 23:10:35: 12000000 INFO @ Mon, 29 Jun 2020 23:10:36: 7000000 INFO @ Mon, 29 Jun 2020 23:10:41: 13000000 INFO @ Mon, 29 Jun 2020 23:10:42: 8000000 INFO @ Mon, 29 Jun 2020 23:10:47: 14000000 INFO @ Mon, 29 Jun 2020 23:10:48: 9000000 INFO @ Mon, 29 Jun 2020 23:10:48: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:10:48: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:10:48: #1 total tags in treatment: 14266730 INFO @ Mon, 29 Jun 2020 23:10:48: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:10:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:10:48: #1 tags after filtering in treatment: 14266730 INFO @ Mon, 29 Jun 2020 23:10:48: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:10:48: #1 finished! INFO @ Mon, 29 Jun 2020 23:10:48: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:10:48: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:10:49: #2 number of paired peaks: 46 WARNING @ Mon, 29 Jun 2020 23:10:49: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:10:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX335507/SRX335507.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335507/SRX335507.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335507/SRX335507.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335507/SRX335507.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:10:53: 10000000 INFO @ Mon, 29 Jun 2020 23:10:59: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:11:05: 12000000 INFO @ Mon, 29 Jun 2020 23:11:11: 13000000 INFO @ Mon, 29 Jun 2020 23:11:16: 14000000 INFO @ Mon, 29 Jun 2020 23:11:18: #1 tag size is determined as 50 bps INFO @ Mon, 29 Jun 2020 23:11:18: #1 tag size = 50 INFO @ Mon, 29 Jun 2020 23:11:18: #1 total tags in treatment: 14266730 INFO @ Mon, 29 Jun 2020 23:11:18: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:11:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:11:18: #1 tags after filtering in treatment: 14266730 INFO @ Mon, 29 Jun 2020 23:11:18: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:11:18: #1 finished! INFO @ Mon, 29 Jun 2020 23:11:18: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:11:18: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:11:19: #2 number of paired peaks: 46 WARNING @ Mon, 29 Jun 2020 23:11:19: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:11:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX335507/SRX335507.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335507/SRX335507.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335507/SRX335507.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335507/SRX335507.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。