Job ID = 1295365 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 35,300,097 reads read : 35,300,097 reads written : 35,300,097 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:05 35300097 reads; of these: 35300097 (100.00%) were unpaired; of these: 11346323 (32.14%) aligned 0 times 20673987 (58.57%) aligned exactly 1 time 3279787 (9.29%) aligned >1 times 67.86% overall alignment rate Time searching: 00:08:05 Overall time: 00:08:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5923296 / 23953774 = 0.2473 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 13:44:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335503/SRX335503.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335503/SRX335503.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335503/SRX335503.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335503/SRX335503.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:44:54: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:44:54: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:44:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335503/SRX335503.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335503/SRX335503.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335503/SRX335503.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335503/SRX335503.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:44:54: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:44:54: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:44:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335503/SRX335503.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335503/SRX335503.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335503/SRX335503.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335503/SRX335503.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:44:54: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:44:54: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:45:03: 1000000 INFO @ Mon, 03 Jun 2019 13:45:04: 1000000 INFO @ Mon, 03 Jun 2019 13:45:06: 1000000 INFO @ Mon, 03 Jun 2019 13:45:12: 2000000 INFO @ Mon, 03 Jun 2019 13:45:14: 2000000 INFO @ Mon, 03 Jun 2019 13:45:18: 2000000 INFO @ Mon, 03 Jun 2019 13:45:21: 3000000 INFO @ Mon, 03 Jun 2019 13:45:24: 3000000 INFO @ Mon, 03 Jun 2019 13:45:29: 3000000 INFO @ Mon, 03 Jun 2019 13:45:30: 4000000 INFO @ Mon, 03 Jun 2019 13:45:35: 4000000 INFO @ Mon, 03 Jun 2019 13:45:38: 5000000 INFO @ Mon, 03 Jun 2019 13:45:41: 4000000 INFO @ Mon, 03 Jun 2019 13:45:44: 5000000 INFO @ Mon, 03 Jun 2019 13:45:47: 6000000 INFO @ Mon, 03 Jun 2019 13:45:53: 5000000 INFO @ Mon, 03 Jun 2019 13:45:54: 6000000 INFO @ Mon, 03 Jun 2019 13:45:55: 7000000 INFO @ Mon, 03 Jun 2019 13:46:05: 8000000 INFO @ Mon, 03 Jun 2019 13:46:05: 6000000 INFO @ Mon, 03 Jun 2019 13:46:05: 7000000 INFO @ Mon, 03 Jun 2019 13:46:15: 9000000 INFO @ Mon, 03 Jun 2019 13:46:16: 8000000 INFO @ Mon, 03 Jun 2019 13:46:17: 7000000 INFO @ Mon, 03 Jun 2019 13:46:24: 10000000 INFO @ Mon, 03 Jun 2019 13:46:26: 9000000 INFO @ Mon, 03 Jun 2019 13:46:29: 8000000 INFO @ Mon, 03 Jun 2019 13:46:33: 11000000 INFO @ Mon, 03 Jun 2019 13:46:36: 10000000 INFO @ Mon, 03 Jun 2019 13:46:41: 9000000 INFO @ Mon, 03 Jun 2019 13:46:41: 12000000 INFO @ Mon, 03 Jun 2019 13:46:47: 11000000 INFO @ Mon, 03 Jun 2019 13:46:50: 13000000 INFO @ Mon, 03 Jun 2019 13:46:52: 10000000 INFO @ Mon, 03 Jun 2019 13:46:57: 12000000 INFO @ Mon, 03 Jun 2019 13:46:58: 14000000 INFO @ Mon, 03 Jun 2019 13:47:04: 11000000 INFO @ Mon, 03 Jun 2019 13:47:06: 13000000 INFO @ Mon, 03 Jun 2019 13:47:07: 15000000 INFO @ Mon, 03 Jun 2019 13:47:14: 12000000 INFO @ Mon, 03 Jun 2019 13:47:15: 16000000 INFO @ Mon, 03 Jun 2019 13:47:16: 14000000 INFO @ Mon, 03 Jun 2019 13:47:25: 17000000 INFO @ Mon, 03 Jun 2019 13:47:26: 13000000 INFO @ Mon, 03 Jun 2019 13:47:26: 15000000 INFO @ Mon, 03 Jun 2019 13:47:35: 18000000 INFO @ Mon, 03 Jun 2019 13:47:35: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:47:35: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:47:35: #1 total tags in treatment: 18030478 INFO @ Mon, 03 Jun 2019 13:47:35: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:47:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:47:36: #1 tags after filtering in treatment: 18030478 INFO @ Mon, 03 Jun 2019 13:47:36: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:47:36: #1 finished! INFO @ Mon, 03 Jun 2019 13:47:36: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:47:36: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:47:37: 16000000 INFO @ Mon, 03 Jun 2019 13:47:37: 14000000 INFO @ Mon, 03 Jun 2019 13:47:37: #2 number of paired peaks: 17 WARNING @ Mon, 03 Jun 2019 13:47:37: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:47:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX335503/SRX335503.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335503/SRX335503.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335503/SRX335503.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335503/SRX335503.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 13:47:46: 17000000 INFO @ Mon, 03 Jun 2019 13:47:48: 15000000 INFO @ Mon, 03 Jun 2019 13:47:55: 18000000 INFO @ Mon, 03 Jun 2019 13:47:56: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:47:56: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:47:56: #1 total tags in treatment: 18030478 INFO @ Mon, 03 Jun 2019 13:47:56: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:47:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:47:56: #1 tags after filtering in treatment: 18030478 INFO @ Mon, 03 Jun 2019 13:47:56: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:47:56: #1 finished! INFO @ Mon, 03 Jun 2019 13:47:56: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:47:56: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:47:58: #2 number of paired peaks: 17 WARNING @ Mon, 03 Jun 2019 13:47:58: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:47:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX335503/SRX335503.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335503/SRX335503.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335503/SRX335503.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335503/SRX335503.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 13:47:59: 16000000 INFO @ Mon, 03 Jun 2019 13:48:09: 17000000 INFO @ Mon, 03 Jun 2019 13:48:19: 18000000 INFO @ Mon, 03 Jun 2019 13:48:19: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:48:19: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:48:19: #1 total tags in treatment: 18030478 INFO @ Mon, 03 Jun 2019 13:48:19: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:48:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:48:20: #1 tags after filtering in treatment: 18030478 INFO @ Mon, 03 Jun 2019 13:48:20: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:48:20: #1 finished! INFO @ Mon, 03 Jun 2019 13:48:20: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:48:20: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:48:21: #2 number of paired peaks: 17 WARNING @ Mon, 03 Jun 2019 13:48:21: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:48:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX335503/SRX335503.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335503/SRX335503.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335503/SRX335503.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335503/SRX335503.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。