Job ID = 1295350 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 32,960,289 reads read : 32,960,289 reads written : 32,960,289 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:33 32960289 reads; of these: 32960289 (100.00%) were unpaired; of these: 4865634 (14.76%) aligned 0 times 22672103 (68.79%) aligned exactly 1 time 5422552 (16.45%) aligned >1 times 85.24% overall alignment rate Time searching: 00:10:33 Overall time: 00:10:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 6367945 / 28094655 = 0.2267 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 13:46:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335498/SRX335498.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335498/SRX335498.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335498/SRX335498.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335498/SRX335498.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:46:22: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:46:22: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:46:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335498/SRX335498.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335498/SRX335498.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335498/SRX335498.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335498/SRX335498.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:46:22: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:46:22: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:46:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335498/SRX335498.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335498/SRX335498.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335498/SRX335498.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335498/SRX335498.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:46:22: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:46:22: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:46:31: 1000000 INFO @ Mon, 03 Jun 2019 13:46:31: 1000000 INFO @ Mon, 03 Jun 2019 13:46:32: 1000000 INFO @ Mon, 03 Jun 2019 13:46:39: 2000000 INFO @ Mon, 03 Jun 2019 13:46:40: 2000000 INFO @ Mon, 03 Jun 2019 13:46:42: 2000000 INFO @ Mon, 03 Jun 2019 13:46:48: 3000000 INFO @ Mon, 03 Jun 2019 13:46:48: 3000000 INFO @ Mon, 03 Jun 2019 13:46:52: 3000000 INFO @ Mon, 03 Jun 2019 13:46:56: 4000000 INFO @ Mon, 03 Jun 2019 13:46:57: 4000000 INFO @ Mon, 03 Jun 2019 13:47:02: 4000000 INFO @ Mon, 03 Jun 2019 13:47:04: 5000000 INFO @ Mon, 03 Jun 2019 13:47:05: 5000000 INFO @ Mon, 03 Jun 2019 13:47:11: 5000000 INFO @ Mon, 03 Jun 2019 13:47:12: 6000000 INFO @ Mon, 03 Jun 2019 13:47:13: 6000000 INFO @ Mon, 03 Jun 2019 13:47:20: 7000000 INFO @ Mon, 03 Jun 2019 13:47:21: 6000000 INFO @ Mon, 03 Jun 2019 13:47:21: 7000000 INFO @ Mon, 03 Jun 2019 13:47:28: 8000000 INFO @ Mon, 03 Jun 2019 13:47:29: 8000000 INFO @ Mon, 03 Jun 2019 13:47:30: 7000000 INFO @ Mon, 03 Jun 2019 13:47:36: 9000000 INFO @ Mon, 03 Jun 2019 13:47:37: 9000000 INFO @ Mon, 03 Jun 2019 13:47:40: 8000000 INFO @ Mon, 03 Jun 2019 13:47:44: 10000000 INFO @ Mon, 03 Jun 2019 13:47:45: 10000000 INFO @ Mon, 03 Jun 2019 13:47:49: 9000000 INFO @ Mon, 03 Jun 2019 13:47:52: 11000000 INFO @ Mon, 03 Jun 2019 13:47:53: 11000000 INFO @ Mon, 03 Jun 2019 13:47:58: 10000000 INFO @ Mon, 03 Jun 2019 13:48:00: 12000000 INFO @ Mon, 03 Jun 2019 13:48:01: 12000000 INFO @ Mon, 03 Jun 2019 13:48:08: 11000000 INFO @ Mon, 03 Jun 2019 13:48:08: 13000000 INFO @ Mon, 03 Jun 2019 13:48:09: 13000000 INFO @ Mon, 03 Jun 2019 13:48:16: 14000000 INFO @ Mon, 03 Jun 2019 13:48:17: 12000000 INFO @ Mon, 03 Jun 2019 13:48:17: 14000000 INFO @ Mon, 03 Jun 2019 13:48:24: 15000000 INFO @ Mon, 03 Jun 2019 13:48:25: 15000000 INFO @ Mon, 03 Jun 2019 13:48:26: 13000000 INFO @ Mon, 03 Jun 2019 13:48:31: 16000000 INFO @ Mon, 03 Jun 2019 13:48:32: 16000000 INFO @ Mon, 03 Jun 2019 13:48:35: 14000000 INFO @ Mon, 03 Jun 2019 13:48:39: 17000000 INFO @ Mon, 03 Jun 2019 13:48:40: 17000000 INFO @ Mon, 03 Jun 2019 13:48:44: 15000000 INFO @ Mon, 03 Jun 2019 13:48:47: 18000000 INFO @ Mon, 03 Jun 2019 13:48:48: 18000000 INFO @ Mon, 03 Jun 2019 13:48:53: 16000000 INFO @ Mon, 03 Jun 2019 13:48:55: 19000000 INFO @ Mon, 03 Jun 2019 13:48:56: 19000000 INFO @ Mon, 03 Jun 2019 13:49:03: 17000000 INFO @ Mon, 03 Jun 2019 13:49:03: 20000000 INFO @ Mon, 03 Jun 2019 13:49:05: 20000000 INFO @ Mon, 03 Jun 2019 13:49:11: 21000000 INFO @ Mon, 03 Jun 2019 13:49:12: 18000000 INFO @ Mon, 03 Jun 2019 13:49:13: 21000000 INFO @ Mon, 03 Jun 2019 13:49:17: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:49:17: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:49:17: #1 total tags in treatment: 21726710 INFO @ Mon, 03 Jun 2019 13:49:17: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:49:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:49:18: #1 tags after filtering in treatment: 21726710 INFO @ Mon, 03 Jun 2019 13:49:18: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:49:18: #1 finished! INFO @ Mon, 03 Jun 2019 13:49:18: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:49:18: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:49:19: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:49:19: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:49:19: #1 total tags in treatment: 21726710 INFO @ Mon, 03 Jun 2019 13:49:19: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:49:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:49:19: #1 tags after filtering in treatment: 21726710 INFO @ Mon, 03 Jun 2019 13:49:19: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:49:19: #1 finished! INFO @ Mon, 03 Jun 2019 13:49:19: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:49:19: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:49:19: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 13:49:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:49:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX335498/SRX335498.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335498/SRX335498.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335498/SRX335498.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335498/SRX335498.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 13:49:21: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 13:49:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:49:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX335498/SRX335498.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335498/SRX335498.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335498/SRX335498.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335498/SRX335498.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 13:49:21: 19000000 INFO @ Mon, 03 Jun 2019 13:49:31: 20000000 INFO @ Mon, 03 Jun 2019 13:49:40: 21000000 INFO @ Mon, 03 Jun 2019 13:49:47: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:49:47: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:49:47: #1 total tags in treatment: 21726710 INFO @ Mon, 03 Jun 2019 13:49:47: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:49:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:49:47: #1 tags after filtering in treatment: 21726710 INFO @ Mon, 03 Jun 2019 13:49:47: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:49:47: #1 finished! INFO @ Mon, 03 Jun 2019 13:49:47: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:49:47: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:49:49: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 13:49:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:49:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX335498/SRX335498.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335498/SRX335498.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335498/SRX335498.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335498/SRX335498.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。