Job ID = 1295345 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 41,603,816 reads read : 41,603,816 reads written : 41,603,816 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:57 41603816 reads; of these: 41603816 (100.00%) were unpaired; of these: 5366980 (12.90%) aligned 0 times 30508124 (73.33%) aligned exactly 1 time 5728712 (13.77%) aligned >1 times 87.10% overall alignment rate Time searching: 00:11:57 Overall time: 00:11:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 9431395 / 36236836 = 0.2603 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 13:55:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335497/SRX335497.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335497/SRX335497.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335497/SRX335497.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335497/SRX335497.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:55:20: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:55:20: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:55:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335497/SRX335497.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335497/SRX335497.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335497/SRX335497.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335497/SRX335497.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:55:20: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:55:20: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:55:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX335497/SRX335497.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX335497/SRX335497.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX335497/SRX335497.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX335497/SRX335497.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 13:55:20: #1 read tag files... INFO @ Mon, 03 Jun 2019 13:55:20: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 13:55:29: 1000000 INFO @ Mon, 03 Jun 2019 13:55:29: 1000000 INFO @ Mon, 03 Jun 2019 13:55:32: 1000000 INFO @ Mon, 03 Jun 2019 13:55:37: 2000000 INFO @ Mon, 03 Jun 2019 13:55:37: 2000000 INFO @ Mon, 03 Jun 2019 13:55:42: 2000000 INFO @ Mon, 03 Jun 2019 13:55:45: 3000000 INFO @ Mon, 03 Jun 2019 13:55:45: 3000000 INFO @ Mon, 03 Jun 2019 13:55:53: 4000000 INFO @ Mon, 03 Jun 2019 13:55:53: 3000000 INFO @ Mon, 03 Jun 2019 13:55:53: 4000000 INFO @ Mon, 03 Jun 2019 13:56:01: 5000000 INFO @ Mon, 03 Jun 2019 13:56:01: 5000000 INFO @ Mon, 03 Jun 2019 13:56:03: 4000000 INFO @ Mon, 03 Jun 2019 13:56:09: 6000000 INFO @ Mon, 03 Jun 2019 13:56:09: 6000000 INFO @ Mon, 03 Jun 2019 13:56:13: 5000000 INFO @ Mon, 03 Jun 2019 13:56:16: 7000000 INFO @ Mon, 03 Jun 2019 13:56:17: 7000000 INFO @ Mon, 03 Jun 2019 13:56:23: 6000000 INFO @ Mon, 03 Jun 2019 13:56:24: 8000000 INFO @ Mon, 03 Jun 2019 13:56:25: 8000000 INFO @ Mon, 03 Jun 2019 13:56:32: 9000000 INFO @ Mon, 03 Jun 2019 13:56:32: 9000000 INFO @ Mon, 03 Jun 2019 13:56:33: 7000000 INFO @ Mon, 03 Jun 2019 13:56:40: 10000000 INFO @ Mon, 03 Jun 2019 13:56:40: 10000000 INFO @ Mon, 03 Jun 2019 13:56:43: 8000000 INFO @ Mon, 03 Jun 2019 13:56:48: 11000000 INFO @ Mon, 03 Jun 2019 13:56:49: 11000000 INFO @ Mon, 03 Jun 2019 13:56:53: 9000000 INFO @ Mon, 03 Jun 2019 13:56:56: 12000000 INFO @ Mon, 03 Jun 2019 13:56:57: 12000000 INFO @ Mon, 03 Jun 2019 13:57:03: 10000000 INFO @ Mon, 03 Jun 2019 13:57:03: 13000000 INFO @ Mon, 03 Jun 2019 13:57:04: 13000000 INFO @ Mon, 03 Jun 2019 13:57:11: 14000000 INFO @ Mon, 03 Jun 2019 13:57:12: 14000000 INFO @ Mon, 03 Jun 2019 13:57:13: 11000000 INFO @ Mon, 03 Jun 2019 13:57:19: 15000000 INFO @ Mon, 03 Jun 2019 13:57:20: 15000000 INFO @ Mon, 03 Jun 2019 13:57:23: 12000000 INFO @ Mon, 03 Jun 2019 13:57:26: 16000000 INFO @ Mon, 03 Jun 2019 13:57:27: 16000000 INFO @ Mon, 03 Jun 2019 13:57:33: 13000000 INFO @ Mon, 03 Jun 2019 13:57:34: 17000000 INFO @ Mon, 03 Jun 2019 13:57:35: 17000000 INFO @ Mon, 03 Jun 2019 13:57:42: 18000000 INFO @ Mon, 03 Jun 2019 13:57:42: 14000000 INFO @ Mon, 03 Jun 2019 13:57:43: 18000000 INFO @ Mon, 03 Jun 2019 13:57:49: 19000000 INFO @ Mon, 03 Jun 2019 13:57:50: 19000000 INFO @ Mon, 03 Jun 2019 13:57:52: 15000000 INFO @ Mon, 03 Jun 2019 13:57:57: 20000000 INFO @ Mon, 03 Jun 2019 13:57:58: 20000000 INFO @ Mon, 03 Jun 2019 13:58:02: 16000000 INFO @ Mon, 03 Jun 2019 13:58:05: 21000000 INFO @ Mon, 03 Jun 2019 13:58:06: 21000000 INFO @ Mon, 03 Jun 2019 13:58:12: 17000000 INFO @ Mon, 03 Jun 2019 13:58:12: 22000000 INFO @ Mon, 03 Jun 2019 13:58:14: 22000000 INFO @ Mon, 03 Jun 2019 13:58:20: 23000000 INFO @ Mon, 03 Jun 2019 13:58:21: 23000000 INFO @ Mon, 03 Jun 2019 13:58:21: 18000000 INFO @ Mon, 03 Jun 2019 13:58:28: 24000000 INFO @ Mon, 03 Jun 2019 13:58:29: 24000000 INFO @ Mon, 03 Jun 2019 13:58:31: 19000000 INFO @ Mon, 03 Jun 2019 13:58:36: 25000000 INFO @ Mon, 03 Jun 2019 13:58:37: 25000000 INFO @ Mon, 03 Jun 2019 13:58:41: 20000000 INFO @ Mon, 03 Jun 2019 13:58:43: 26000000 INFO @ Mon, 03 Jun 2019 13:58:44: 26000000 INFO @ Mon, 03 Jun 2019 13:58:49: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:58:49: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:58:49: #1 total tags in treatment: 26805441 INFO @ Mon, 03 Jun 2019 13:58:49: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:58:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:58:50: #1 tags after filtering in treatment: 26805441 INFO @ Mon, 03 Jun 2019 13:58:50: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:58:50: #1 finished! INFO @ Mon, 03 Jun 2019 13:58:50: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:58:50: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:58:51: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:58:51: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:58:51: #1 total tags in treatment: 26805441 INFO @ Mon, 03 Jun 2019 13:58:51: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:58:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:58:51: 21000000 INFO @ Mon, 03 Jun 2019 13:58:51: #1 tags after filtering in treatment: 26805441 INFO @ Mon, 03 Jun 2019 13:58:51: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:58:51: #1 finished! INFO @ Mon, 03 Jun 2019 13:58:51: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:58:51: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:58:52: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 13:58:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:58:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX335497/SRX335497.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335497/SRX335497.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335497/SRX335497.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335497/SRX335497.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 13:58:53: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 13:58:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:58:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX335497/SRX335497.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335497/SRX335497.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335497/SRX335497.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335497/SRX335497.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 13:59:00: 22000000 INFO @ Mon, 03 Jun 2019 13:59:10: 23000000 INFO @ Mon, 03 Jun 2019 13:59:20: 24000000 INFO @ Mon, 03 Jun 2019 13:59:29: 25000000 INFO @ Mon, 03 Jun 2019 13:59:39: 26000000 INFO @ Mon, 03 Jun 2019 13:59:46: #1 tag size is determined as 50 bps INFO @ Mon, 03 Jun 2019 13:59:46: #1 tag size = 50 INFO @ Mon, 03 Jun 2019 13:59:46: #1 total tags in treatment: 26805441 INFO @ Mon, 03 Jun 2019 13:59:46: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 13:59:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 13:59:47: #1 tags after filtering in treatment: 26805441 INFO @ Mon, 03 Jun 2019 13:59:47: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 13:59:47: #1 finished! INFO @ Mon, 03 Jun 2019 13:59:47: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 13:59:47: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 13:59:49: #2 number of paired peaks: 0 WARNING @ Mon, 03 Jun 2019 13:59:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 13:59:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX335497/SRX335497.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335497/SRX335497.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335497/SRX335497.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX335497/SRX335497.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。